Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC NC_000913 1,757,082 IS5 (–) +4 bp 17.2% coding (1385‑1388/1413 nt) pykF → pyruvate kinase I

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1397238NA (NA)7 (0.080) 7/256 NT 14.2% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1757085 41 (0.470)coding (1388/1413 nt) pykF pyruvate kinase I
* ? NC_000913 1757082 =41 (0.470)10 (0.110) 7/256 NT 19.1% coding (1385/1413 nt) pykF pyruvate kinase I
?NC_000913 = 2102943 NA (NA)noncoding (1/1195 nt) IS5 repeat region

TAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1397103‑1397238
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTTGTGTGCCAGACCGCTCTGCAGAGCCAGTTCTTTACCCAGACGGTAGAAATCATCAGTAGAAGTGATCTCTTTAACAAGCTG  <  NC_000913/1757085‑1756949
                                                                                                                                                                                                                                                                                 
TAACGCCTGAAATGGCGAAGAAACGGTCTAAATAGGCTGAGTCAAGGCATTTACGGGAGAAAAAATCGGCTCCAAAATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAG                                                                                                                                       <  2:436372/140‑1
        TAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCG                                                                                                                               >  1:103271/1‑140
                          TCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCACCA                                                                                                             <  2:178878/140‑1
                                aAGGCAGAATCAAGGCAATTACGGGAGAAAAAATCCGCTCAAACATGAAGAAATGAAAAGACAGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACC                                                                                                       <  2:1287073/139‑1
                                      GATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACT                                                                                                 <  2:78903/140‑1
                                                                     CTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTTGTGTGCCAGACCGCTCT                                                                  >  2:384827/1‑140
                                                                                    ATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTTGTGTGCCAGACCGCTCTGCAGAGCCAGTTCTT                                                   >  2:633914/1‑140
                                                                                                                      CGCTTATTCGCACCTTCCTTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTTGTGTGCCAGACCGCTCTGCAGAGCCAGTTCTTTACCCAGACGGTAGAAATCATCAGTAGAAGTGAT                 <  1:765287/140‑1
                                                                                                                                     TCCTTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTTGTGTGCCAGACCGCTCTGCAGAGCCAGTTCTTTACCCAGACGGTAGAAATCATCAGTAGAAGTGATCTCTTTAACAAGCTG  >  1:952913/1‑140
                                                                                                                                     TCCTTAGTAGTGCCGCTCGGTACCAGAGCACCAGAAACCATAACTACAACGTCACCTTTGTGTGCCAGACCGCTCTGCAGAGCCAGTTCTTTACCCAGACGGTAGAAATCATCAGTAGAAGTGATCTCTTTAACAAGCTG  >  1:223837/1‑140
                                                                                                                                                                                                                                                                                 
TAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1397103‑1397238
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGTAGTGCCGCTCGGTACCAGTGCACCAGAAACCATAACTACAACGTCACCTTTGTGTGCCAGACCGCTCTGCAGAGCCAGTTCTTTACCCAGACGGTAGAAATCATCAGTAGAAGTGATCTCTTTAACAAGCTG  <  NC_000913/1757085‑1756949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.