Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,562,459 C→A 5.1% G73C (GGC→TGC)  yjiJ ← DUF1228 family putative inner membrane MFS superfamily transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,562,4590CA 5.1% 333.7 / 2.3 95G73C (GGC→TGC) yjiJDUF1228 family putative inner membrane MFS superfamily transporter
Reads supporting (aligned to +/- strand):  ref base C (37/52);  new base A (2/4);  total (39/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCA  >  NC_000913/4562320‑4562596
                                                                                                                                           |                                                                                                                                         
cTGGCGACACCCGCCAGGACGCGAATAAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGcc                                                                                                                                           >  1:149707/1‑140 (MQ=255)
   gCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCaaa                                                                                                                                        >  1:459500/1‑140 (MQ=255)
   gCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCaaa                                                                                                                                        >  1:459501/1‑140 (MQ=255)
    cGACACCCGCCAGGTCGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCaaaa                                                                                                                                       <  2:317618/140‑1 (MQ=255)
    cGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCATCTACCCGCTCGCCAGGGCAGAAGCCAACAGATATGGGCGCAGGCGCGATGGCTGGTGTAATGCGCCaaaa                                                                                                                                       <  2:1423755/140‑1 (MQ=255)
        acCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCAAAAAGaaa                                                                                                                                   >  1:232202/1‑140 (MQ=255)
        acCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGACAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAACTGCGCAAAAAGaaa                                                                                                                                   >  1:232227/1‑140 (MQ=255)
             ccAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGc                                                                                                                              >  2:1668442/1‑140 (MQ=255)
             ccAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAAACCGCTCGCCAGGGCAGAAGCCAACAGAAGTGGGCGCAGGCGCGTTGGCTGGTGAAGTGCGCCAAACGAAAAGAGc                                                                                                                              >  2:933050/1‑140 (MQ=255)
              cAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCa                                                                                                                             <  1:1476546/140‑1 (MQ=255)
              cAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCa                                                                                                                             <  1:1476161/140‑1 (MQ=255)
                    gcgAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCTAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTgc                                                                                                                       <  1:108025/140‑1 (MQ=255)
                    gcgAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTgc                                                                                                                       <  1:1273980/140‑1 (MQ=255)
                    gcgAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTgc                                                                                                                       <  1:524526/140‑1 (MQ=255)
                    gcgAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCACTCGCCAGGGCAGAAGCCAACTGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTgc                                                                                                                       <  2:1280544/140‑1 (MQ=255)
                           aaTAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGccagccag                                                                                                                <  1:1513946/140‑1 (MQ=255)
                           aaTAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGccagccag                                                                                                                <  2:1254792/140‑1 (MQ=255)
                            aTAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGa                                                                                                               <  2:676037/140‑1 (MQ=255)
                                cacCAGACTAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGATAGCCAGGGCAGAAGCCATCAGAAATGGGCGCAGGCGCGATGGATGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATAcc                                                                                                           <  2:925801/140‑1 (MQ=255)
                                cacCAGAATAAATGGCGTCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGTCAGATGCCAACAGAAATGGGCGCAGGCGCGATGTCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATAcc                                                                                                           <  2:925774/140‑1 (MQ=255)
                                cacCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCTCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATAcc                                                                                                           <  2:926178/140‑1 (MQ=255)
                                cacCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGAAAcc                                                                                                           >  2:1332801/1‑140 (MQ=255)
                                cacCAGAATAAATGGCGGCAACCATGCCAATGCGATGATCAACAACCCGATCGCCAGGGAAGAAGCCAACAGAAATGGGCGCGGGCGCGATGGCTGGTGAAATGCGCCAAAAGACAAAAGCAGACTGCCAGCCAGATAcc                                                                                                           >  2:87702/1‑140 (MQ=255)
                                     gAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCa                                                                                                      >  2:549170/1‑140 (MQ=255)
                                             ggcggcAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATCGCAGACTGCCAGCCAGATACCCCGCATAGTTGcc                                                                                              <  1:783937/140‑1 (MQ=255)
                                              gcggcAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGGTGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCg                                                                                             <  1:703818/140‑1 (MQ=255)
                                              gcggcAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCg                                                                                             <  1:703850/140‑1 (MQ=255)
                                                ggcAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGTCAGCCAGATACCCCGCATAGTTGCCGCt                                                                                           >  1:170184/1‑140 (MQ=255)
                                                ggcAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCt                                                                                           >  1:654930/1‑140 (MQ=255)
                                                ggcAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCt                                                                                           >  2:1653894/1‑140 (MQ=255)
                                                ggcAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCt                                                                                           >  2:1313517/1‑140 (MQ=255)
                                                ggcAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCt                                                                                           <  2:735524/140‑1 (MQ=255)
                                                ggcAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCt                                                                                           >  2:899502/1‑140 (MQ=255)
                                                ggcAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCt                                                                                           >  1:1337561/1‑140 (MQ=255)
                                                ggcAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCt                                                                                           >  1:216783/1‑140 (MQ=255)
                                                ggcAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGACAGCCAGATACCCCGCATAGTTGCCGCt                                                                                           >  1:169992/1‑140 (MQ=255)
                                                  ctacattGCCATTGCGAGGATAAACAACCCTCTCGACAGTGCAGAAGACTTGAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCAATAAGAAAATTGCTGTCTGACAGCCTGATACCCCGCATAGTTGCCGCTgg                                                                                         <  2:1353480/134‑1 (MQ=255)
                                                     ccATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCaa                                                                                      <  2:1450125/140‑1 (MQ=255)
                                                      cATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAAt                                                                                     <  1:307803/140‑1 (MQ=255)
                                                      cATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAAt                                                                                     <  1:310998/140‑1 (MQ=255)
                                                              tGCGAGGATCAACAACCCGCTCGCCAGGGCCGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACgaga                                                                             >  2:454176/1‑140 (MQ=255)
                                                               gCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGagag                                                                            >  1:687740/1‑140 (MQ=255)
                                                               gCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGGGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGagag                                                                            >  1:687670/1‑140 (MQ=255)
                                                                cGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGc                                                                           >  1:1432478/1‑140 (MQ=255)
                                                                cGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGc                                                                           >  2:1504779/1‑140 (MQ=255)
                                                                        aacaacCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTaaa                                                                   <  1:1492033/140‑1 (MQ=255)
                                                                               cGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGACATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTCAATGACAAc                                                            >  1:137993/1‑140 (MQ=255)
                                                                               cGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGACAAc                                                            >  1:137722/1‑140 (MQ=255)
                                                                               cGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAAc                                                            >  2:259751/1‑140 (MQ=255)
                                                                                gCTCGCCAGTGCAGAAGCCATCAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAATATAGGAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCTCGTGAGCTGGCTAATTGAAATCg                                                           <  2:143176/140‑1 (MQ=255)
                                                                                gCTCGCCAGGTCAGAAGCCAACAGATTTGGGCGCTGGCGCGATGGTTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCTTAGTTGCCGCTGGCAATCCTCGAGAGCTGGCTAAATGAAAACg                                                           <  2:741123/140‑1 (MQ=255)
                                                                                gCTCGACAGGGCAGAAGCCAACTGAATTGGGCGCTGGAGAGATGGCTGGTGATATTCGCCAAAAGAAAATAGCAGACTGCCAGCCATATACCCCGCTTAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAATTGAAAACg                                                           <  2:143143/140‑1 (MQ=255)
                                                                                gCTCGACAGGGCAGAAGCCAACTGAAATGTGCGCAGGCGCGTTGGCTGGTGAAATGCGCATAATGTAAATATCAGACTGCCAGCCAGTTACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGATAACg                                                           <  2:487274/140‑1 (MQ=255)
                                                                                 cTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGa                                                          >  1:1379588/1‑140 (MQ=255)
                                                                                 cTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGa                                                          <  1:142224/140‑1 (MQ=255)
                                                                                       aGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTc                                                    <  2:427884/140‑1 (MQ=255)
                                                                                         ggCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCg                                                  <  1:531557/140‑1 (MQ=255)
                                                                                         ggCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCg                                                  >  2:1142025/1‑140 (MQ=255)
                                                                                              aaGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTc                                                    <  1:685558/133‑1 (MQ=255)
                                                                                              aaGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTc                                                    >  2:685558/1‑133 (MQ=255)
                                                                                              aaGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCcatc                                             >  1:1113864/1‑140 (MQ=255)
                                                                                              aaGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCcatc                                             >  2:1685780/1‑140 (MQ=255)
                                                                                              aaGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCcatc                                             >  1:1114453/1‑140 (MQ=255)
                                                                                                 ccAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATg                                          <  1:764017/140‑1 (MQ=255)
                                                                                                 ccAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATg                                          <  1:1417859/140‑1 (MQ=255)
                                                                                                  cAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGa                                         <  2:496423/140‑1 (MQ=255)
                                                                                                    aCAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCTAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACg                                       <  1:1641285/140‑1 (MQ=255)
                                                                                                    aCAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACg                                       <  1:1229083/140‑1 (MQ=255)
                                                                                                      aGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGTCTGCCAGCCAGATACCACGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACggg                                     <  2:1350992/140‑1 (MQ=255)
                                                                                                      aGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACggg                                     <  2:404265/140‑1 (MQ=255)
                                                                                                      aGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACggg                                     <  1:742901/140‑1 (MQ=255)
                                                                                                      aGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCAAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACggg                                     <  2:232202/140‑1 (MQ=255)
                                                                                                      aGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCAAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACggg                                     <  2:232227/140‑1 (MQ=255)
                                                                                                          aTGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACggg                                     <  2:1628832/136‑1 (MQ=255)
                                                                                                          aTGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACggg                                     >  1:1628832/1‑136 (MQ=255)
                                                                                                          aTGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAAc                                 >  2:767778/1‑140 (MQ=255)
                                                                                                           tGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACa                                <  1:1040608/140‑1 (MQ=255)
                                                                                                           tGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACa                                <  1:1041116/140‑1 (MQ=255)
                                                                                                           tGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACa                                <  1:1041285/140‑1 (MQ=255)
                                                                                                           tGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACa                                <  1:1220861/140‑1 (MQ=255)
                                                                                                           tGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACa                                <  1:1668442/140‑1 (MQ=255)
                                                                                                               cgcAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAgg                            >  2:1535058/1‑140 (MQ=255)
                                                                                                                gcAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGc                           >  1:614673/1‑140 (MQ=255)
                                                                                                                gcAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGc                           >  1:1020597/1‑140 (MQ=255)
                                                                                                                    gcgcGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGtat                       >  1:1652381/1‑140 (MQ=255)
                                                                                                                            gCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAg               <  1:1679369/140‑1 (MQ=255)
                                                                                                                              tGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCg             >  1:263110/1‑140 (MQ=255)
                                                                                                                                  gAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCt         <  1:1640271/140‑1 (MQ=255)
                                                                                                                                   aaaTGCGCCATAAGAATATAGCTGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTa        <  2:1088169/140‑1 (MQ=255)
                                                                                                                                   aaaTGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTa        <  2:1086752/140‑1 (MQ=255)
                                                                                                                                       gcgcCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAAcc    <  2:1330161/140‑1 (MQ=255)
                                                                                                                                       gcgcCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAAcc    <  2:213135/140‑1 (MQ=255)
                                                                                                                                        cgcCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCTCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTATAccc   <  2:1074539/140‑1 (MQ=255)
                                                                                                                                        cgcCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAAccc   <  2:1497311/140‑1 (MQ=255)
                                                                                                                                        cgcCAAAAGAAAATAGCAGACTGCCACCCAGATACCCCGCATAGTTGCCTCTGGCAATCGACGAGAGCAGTCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGGGTATAAAGGAAGCGGCGGAAAccc   >  2:6037/1‑140 (MQ=255)
                                                                                                                                         gcCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCa  <  1:104484/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGGCAGAAGCCAACAGAAATGGGCGCAGGCGCGATGGCTGGTGAAATGCGCCAAAAGAAAATAGCAGACTGCCAGCCAGATACCCCGCATAGTTGCCGCTGGCAATCCACGAGAGCTGGCTAAATGAAAACGATCCTTCCGCCATCATGACGGGCAACATAGGCGTATAAAGGAAGCGGCCTAAACCCA  >  NC_000913/4562320‑4562596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑