Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,633,657 C→T 59.4% C30Y (TGC→TAC)  yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,633,6570CT59.4% 38.7 / 80.1 64C30Y (TGC→TAC) yjjXnon‑canonical purine NTP phosphatase, ITPase/XTPase
Reads supporting (aligned to +/- strand):  ref base C (11/15);  new base T (19/19);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

TCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGC  >  NC_000913/4633519‑4633796
                                                                                                                                          |                                                                                                                                           
tCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCa                                                                                                                                            <  1:1219248/140‑1 (MQ=255)
tCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCa                                                                                                                                            <  1:238459/140‑1 (MQ=255)
       ccACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCt                                                                                                                                     >  2:1141895/1‑140 (MQ=255)
        cACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTTCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCtt                                                                                                                                    >  2:1347574/1‑140 (MQ=255)
        cACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCtt                                                                                                                                    >  2:317807/1‑140 (MQ=255)
        cACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCtt                                                                                                                                    >  2:1023324/1‑140 (MQ=255)
                   cAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGAtc                                                                                                                         <  1:122670/140‑1 (MQ=255)
                     gCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGAtctc                                                                                                                       >  2:346576/1‑140 (MQ=255)
                     gCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGAtctc                                                                                                                       >  1:213140/1‑140 (MQ=255)
                     gCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGAtctc                                                                                                                       >  1:213084/1‑140 (MQ=255)
                         ctcccaGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCCTTTCCTCACTGCCAAAGGGCAGTTCCGGTACACCGCACTCGACGGCAACGGATGCAATATGGTAGGATCCGTCGCCGAAGATATCGTGa                                                                                                                   <  2:1624426/135‑1 (MQ=255)
                         ctGGAAGTAAACGGCGGGCATTGGCTTCCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGa                                                                                                                   <  2:1649494/140‑1 (MQ=255)
                         ctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGa                                                                                                                   <  2:851467/140‑1 (MQ=255)
                         ctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGa                                                                                                                   <  2:783187/140‑1 (MQ=255)
                                tAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCt                                                                                                            >  1:1532635/1‑140 (MQ=255)
                                                     cagATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAg                                                                                       >  1:265500/3‑140 (MQ=255)
                                                          ttCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGa                                                                                  <  1:1549354/140‑1 (MQ=255)
                                                          ttCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGa                                                                                  <  1:1134770/140‑1 (MQ=255)
                                                          ttCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGa                                                                                  <  1:589042/140‑1 (MQ=255)
                                                              tGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtgg                                                                              >  2:1455971/1‑140 (MQ=255)
                                                                       gcgcgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGcac                                                                     <  2:513614/140‑1 (MQ=255)
                                                                        cgcgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGcaca                                                                    <  2:46388/140‑1 (MQ=255)
                                                                        cgcgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGcaca                                                                    <  2:46722/140‑1 (MQ=255)
                                                                          cgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGa                                                                  <  2:1057583/140‑1 (MQ=255)
                                                                          cgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGa                                                                  <  1:150756/140‑1 (MQ=255)
                                                                          cgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGa                                                                  <  1:149227/140‑1 (MQ=255)
                                                                          cgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGa                                                                  <  1:120656/140‑1 (MQ=255)
                                                                               ccTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACt                                                             <  2:1467307/140‑1 (MQ=255)
                                                                                cTCACTGCCATAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTAGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACtt                                                            <  2:989529/140‑1 (MQ=255)
                                                                                cTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCTATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACtt                                                            <  2:1216205/140‑1 (MQ=255)
                                                                                     ttccAAAGGGCAGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTAAGCGGGAATGGTGGTCGCACAGACCACTTGGTGc                                                       >  2:441378/3‑140 (MQ=255)
                                                                                     tGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGc                                                       >  2:1175110/1‑140 (MQ=255)
                                                                                        cAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATa                                                    <  2:1085466/140‑1 (MQ=255)
                                                                                          aaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAAt                                                  <  2:1271729/140‑1 (MQ=255)
                                                                                           aGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTACGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCGGTATTTTAGCGGGATTGGTGGTCGCTCAGACGACTGGGTGCATAATc                                                 >  2:291806/1‑140 (MQ=255)
                                                                                           aGGGCTGTTCCGGTACACAGCTCTTCAAGGGAACGGATGCACTAAGGCAGGATCCTTCGCGGTAGATCTCGTGACATGCCTGCAGGATGGCCGGAGTGTTAGCGGGATGGGTGGTCGCTCAGACAACTTGGTGcctgagc                                                 >  2:1429678/1‑134 (MQ=255)
                                                                                             ggCTGTTCCGGTACACCGCACTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAg                                               <  1:685662/140‑1 (MQ=255)
                                                                                               cTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCa                                             >  1:734787/1‑140 (MQ=255)
                                                                                               cTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCa                                             >  1:401209/1‑140 (MQ=255)
                                                                                                 gTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCAtt                                           >  1:817327/1‑140 (MQ=255)
                                                                                                 gTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCAtt                                           >  1:322796/1‑140 (MQ=255)
                                                                                                      ggTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCa                                      <  2:1556519/140‑1 (MQ=255)
                                                                                                        tACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGa                                    >  2:621433/1‑140 (MQ=255)
                                                                                                            ccGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAt                                >  1:1517702/1‑140 (MQ=255)
                                                                                                               ctctTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTaa                             <  2:366776/140‑1 (MQ=255)
                                                                                                               ctctTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTaa                             <  2:366552/140‑1 (MQ=255)
                                                                                                               ctctTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTaa                             >  2:1525674/1‑140 (MQ=255)
                                                                                                               ctctTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTaa                             <  2:541167/140‑1 (MQ=255)
                                                                                                               ctctCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGGATTTTAGCGGGAGTGGGGGTCGCACTGCCAACTTGGTGCATAATCAGCATTCCTCAGAAAATTaa                             >  1:1482011/1‑140 (MQ=255)
                                                                                                               ctctCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTg                                                        >  1:1176797/1‑113 (MQ=255)
                                                                                                               ctctCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTg                                                        <  2:1176797/113‑1 (MQ=255)
                                                                                                               ctctCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATa                                                    <  1:590538/117‑1 (MQ=255)
                                                                                                               ctctCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATa                                                    >  2:590538/1‑117 (MQ=255)
                                                                                                                    gACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTa                        >  1:590945/1‑140 (MQ=255)
                                                                                                                       ggCAACGGCTGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGACTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGAATAATCAGCATTACTCAGAAAATTAACGTTAcag                     <  2:631283/140‑1 (MQ=255)
                                                                                                                       ggCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcag                     <  2:1299278/140‑1 (MQ=255)
                                                                                                                       ggCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCATGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcag                     <  2:631240/140‑1 (MQ=255)
                                                                                                                            cGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGta                >  1:508262/1‑140 (MQ=255)
                                                                                                                            cGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGta                >  1:1527644/1‑140 (MQ=255)
                                                                                                                            cGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGta                <  1:835301/140‑1 (MQ=255)
                                                                                                                            cGGATGCAATATGGCAGGATACTAACACGAAGATCTCGATAAAAGACTGCAGAAACGACTGTATATTATCCTGAAAGCTGGTCTAACACACAACTAAGAGCAAAAACACCAATACTCAGAAAAAAAACGTTACAGCAGta                >  1:1674303/1‑140 (MQ=255)
                                                                                                                                tGCAATAGGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGACAATTAACGTTACAGCAGTATACg            >  2:1113320/1‑140 (MQ=255)
                                                                                                                                       tGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGaaaaaa     >  1:1189765/1‑140 (MQ=255)
                                                                                                                                         gCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAg   <  1:137216/140‑1 (MQ=255)
                                                                                                                                          cAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGc  <  1:502142/140‑1 (MQ=255)
                                                                                                                                          cAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGc  <  1:541435/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAAAGC  >  NC_000913/4633519‑4633796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: