Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 919,889 G→T 5.4% D219Y (GAT→TAT)  macA → macrolide transporter membrane fusion protein (MFP) component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913919,8890GT 5.4% 403.9 / 5.5 111D219Y (GAT→TAT) macAmacrolide transporter membrane fusion protein (MFP) component
Reads supporting (aligned to +/- strand):  ref base G (48/57);  new base T (3/3);  total (51/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 111 total aligned reads displayed.

CTCTCGATACGGCTAAAACCAATCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAGGATGTACTACCGA  >  NC_000913/919752‑920027
                                                                                                                                         |                                                                                                                                          
ctctcGATACGGCTAAAACCAATCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGat                                                                                                                                          >  2:135257/1‑140 (MQ=255)
ctctcGATACGGCTAAAACCAATCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGat                                                                                                                                          >  1:1691975/1‑140 (MQ=255)
  ctcGATACGGCTAAAACCAATCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGatat                                                                                                                                        <  2:1586298/140‑1 (MQ=255)
  ctcGATACGGCTAAAACCAATCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGatat                                                                                                                                        >  1:238376/1‑140 (MQ=255)
     gATACGGCTAAAACCACTCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAg                                                                                                                                     <  2:588132/140‑1 (MQ=255)
          ggCTAAAACCAATCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCa                                                                                                                                >  2:753085/1‑140 (MQ=255)
           gCTAAAACCAATCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCAt                                                                                                                               <  1:146799/140‑1 (MQ=255)
           gCTAAAACCAATCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCAt                                                                                                                               >  2:84833/1‑140 (MQ=255)
            cTAAAACCAATCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATg                                                                                                                              <  2:1283713/140‑1 (MQ=255)
            cTAAAACCAATCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATg                                                                                                                              <  2:1577798/140‑1 (MQ=255)
               aaaCCAATCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTg                                                                                                                           >  2:518918/1‑140 (MQ=255)
               aaaCCAATCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTg                                                                                                                           >  2:551527/1‑140 (MQ=255)
                     aTCTCGATTACACTCGCATCGTTGCCCCGATGGACGGGGAAGTCTCGCAAATCACCACTCTGCAAGGCCAGACGGTGAGTGCCGCTCAACAAGCTCCTACCATTCGCTCGCTGGCAGAGTTGTGCGCGATGCTGGTcaac                                                                                                                     >  2:1668204/1‑139 (MQ=255)
                      tctcGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTaaatg                                                                                                                    >  1:451443/1‑138 (MQ=255)
                      tctcGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAg                                                                                                                    >  1:935866/1‑140 (MQ=255)
                      tctcGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAg                                                                                                                    >  1:91722/1‑140 (MQ=255)
                        tcGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAgcg                                                                                                                  <  2:765813/140‑1 (MQ=255)
                        tcGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAgcg                                                                                                                  <  1:1046507/140‑1 (MQ=255)
                          gATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAgcgct                                                                                                                >  1:1564947/1‑139 (MQ=255)
                          gATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCa                                                                                                                >  1:313650/1‑140 (MQ=255)
                           aTTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAg                                                                                                               >  1:391255/1‑140 (MQ=255)
                                    gCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCATATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGaa                                                                                                      >  1:842813/1‑140 (MQ=255)
                                    gCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCATATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGaa                                                                                                      >  1:842866/1‑140 (MQ=255)
                                    gCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCATATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGaa                                                                                                      >  1:842731/1‑140 (MQ=255)
                                     cATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTTACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAg                                                                                                     <  2:1343257/140‑1 (MQ=255)
                                     cATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAg                                                                                                     <  1:378159/140‑1 (MQ=255)
                                     cATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAg                                                                                                     <  1:811437/140‑1 (MQ=255)
                                     cATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAg                                                                                                     <  2:1591286/140‑1 (MQ=255)
                                     cATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAg                                                                                                     <  2:95234/140‑1 (MQ=255)
                                     cATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAg                                                                                                     <  2:1588931/140‑1 (MQ=255)
                                     cATCGGTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCTCGGCCAGACGGTGAGTGCCGCTCAACACGCTCCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAg                                                                                                     <  1:378174/140‑1 (MQ=255)
                                      aTCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGAATGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCGTGCTGGTAAAAGAGCAGGTTGCTGAAGc                                                                                                    >  2:1117980/1‑140 (MQ=255)
                                         gTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGa                                                                                                 <  1:384842/140‑1 (MQ=255)
                                           tGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATg                                                                                               <  2:134614/140‑1 (MQ=255)
                                                cGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATc                                                                                          <  2:1169268/140‑1 (MQ=255)
                                                cGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATc                                                                                          <  1:1475529/140‑1 (MQ=255)
                                                cGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATc                                                                                          >  1:1165864/1‑140 (MQ=255)
                                                cGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATc                                                                                          <  2:1395134/140‑1 (MQ=255)
                                                  aTGGCCGGTGAAGTCACGCAAATCACAACACTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCa                                                                                        >  2:1279399/1‑140 (MQ=255)
                                                  aTGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTATGAAGCGGATGTAATCCa                                                                                        >  2:1039409/1‑140 (MQ=255)
                                                        ggggAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGAGTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGGTTCTGAAGCGGATGTAATCCACCTGaa                                                                                  >  2:252755/1‑140 (MQ=255)
                                                             aGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCgg                                                                             <  1:1374430/140‑1 (MQ=255)
                                                                   gCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCaaa                                                                       <  1:1265716/140‑1 (MQ=255)
                                                                       aTCACCTCTCTGCAAGGCCAGACGGTTATTGGCGCACACAAAGGACCTACCATTCTGACGCTGGCAGATATGAGCGACATGCAGGTAAAAGCGCAGGTTTCTGAAGAGGATGTAATCCACCTGAAGCCGGGGCATAAAGc                                                                   <  2:435204/140‑1 (MQ=255)
                                                                       aTCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGc                                                                   <  1:968562/140‑1 (MQ=255)
                                                                       aTCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGc                                                                   <  1:1511517/140‑1 (MQ=255)
                                                                       aTCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGc                                                                   <  2:887694/140‑1 (MQ=255)
                                                                       aTCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGc                                                                   <  1:183927/140‑1 (MQ=255)
                                                                       aTCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGc                                                                   <  2:1437058/140‑1 (MQ=255)
                                                                               tctGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTa                                                           <  1:389757/140‑1 (MQ=255)
                                                                               tctGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTa                                                           <  1:390158/140‑1 (MQ=255)
                                                                               tctGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTa                                                           <  2:187597/140‑1 (MQ=255)
                                                                               tctGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTa                                                           <  1:1470874/140‑1 (MQ=255)
                                                                               tcagCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTa                                                           <  1:1470891/137‑1 (MQ=255)
                                                                                 tGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACg                                                         >  1:1624734/1‑140 (MQ=255)
                                                                                 tGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACg                                                         >  2:751319/1‑140 (MQ=255)
                                                                                 tGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGCGCAAAAAGCCTGGTTTACg                                                         >  2:976113/1‑140 (MQ=255)
                                                                                 agcaaggcaagACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGAGGGCAGATATGAGCTCCATGCTGGTAAACGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGGCGGGGCAAAAAGCCTGGTTTACg                                                         <  2:88229/139‑1 (MQ=255)
                                                                                      ggCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCt                                                    <  2:58845/140‑1 (MQ=255)
                                                                                      ggCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCt                                                    >  2:1057313/1‑140 (MQ=255)
                                                                                      ggCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCt                                                    >  2:935613/1‑140 (MQ=255)
                                                                                         cAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTgg                                                 <  2:872383/140‑1 (MQ=255)
                                                                                           gACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCg                                               >  2:982583/1‑140 (MQ=255)
                                                                                           gACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCg                                               >  2:1158889/1‑140 (MQ=255)
                                                                                           gACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCg                                               >  2:1107288/1‑140 (MQ=255)
                                                                                           gACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCg                                               >  2:1107240/1‑140 (MQ=255)
                                                                                                tGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCa                                          <  1:449641/140‑1 (MQ=255)
                                                                                                tGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCa                                          <  1:566982/140‑1 (MQ=255)
                                                                                                  aTTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACt                                        >  1:1096594/1‑140 (MQ=255)
                                                                                                      ccGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGAcg                                    <  1:372019/140‑1 (MQ=255)
                                                                                                        gCACAACAAGCACCGAACATTCTGACGCTGGCATATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGAcgcg                                  <  2:842813/140‑1 (MQ=255)
                                                                                                        gCACAACAAGCACCGAACATTCTGACGCTGGCATATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGAcgcg                                  <  2:842731/140‑1 (MQ=255)
                                                                                                        gCACAACAAGCACCGAACATTCTGACGCTGGCATATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGAcgcg                                  <  2:842866/140‑1 (MQ=255)
                                                                                                        gCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGAcgcg                                  <  1:461082/140‑1 (MQ=255)
                                                                                                        gCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGAcgcg                                  >  1:1537956/1‑140 (MQ=255)
                                                                                                          acaacaAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCt                                >  2:121805/1‑140 (MQ=255)
                                                                                                                  cACCTAAGATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTATGAAGCGGATGTTATCCACCTGAAGCCGGGGCAAAAATCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAgggg                        <  2:899987/140‑1 (MQ=255)
                                                                                                                  cACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAgggg                        <  2:935866/140‑1 (MQ=255)
                                                                                                                    ccGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCa                      <  2:1150131/140‑1 (MQ=255)
                                                                                                                      gAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAATGCGCAGGTGTCTGAAGCGGATGTAATCCGCCTGAAGCCGGGGCAAAATGCCTGGTTGACGGTGCTTGGCGATCCACTGACGCGCTAGGGGGGGcgct                    >  2:391437/1‑137 (MQ=255)
                                                                                                                      gAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCaaa                    >  2:116212/1‑140 (MQ=255)
                                                                                                                      gAACATTCTGACGCTGGCAGATATGAGCGCCATGCTAGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCaaa                    >  1:177848/1‑140 (MQ=255)
                                                                                                                       aaCATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAAt                   <  1:1387664/140‑1 (MQ=255)
                                                                                                                       aaCATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAAt                   <  1:54754/140‑1 (MQ=255)
                                                                                                                       aaCATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAAt                   <  1:217783/140‑1 (MQ=255)
                                                                                                                          aTTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGAAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCaa                <  2:687579/140‑1 (MQ=255)
                                                                                                                            tCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAgg              >  1:12916/1‑140 (MQ=255)
                                                                                                                            tCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAgg              <  1:1203918/140‑1 (MQ=255)
                                                                                                                            tCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAgg              >  2:1248079/1‑140 (MQ=255)
                                                                                                                            tCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAgg              <  1:575962/140‑1 (MQ=255)
                                                                                                                            tCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAgg              >  2:1668179/1‑140 (MQ=255)
                                                                                                                               gACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTa                                                           <  2:724743/92‑1 (MQ=255)
                                                                                                                               gACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTa                                                           >  1:724743/1‑92 (MQ=255)
                                                                                                                               gACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAGGATg           >  2:842557/1‑140 (MQ=255)
                                                                                                                               gACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAGGATg           >  2:688198/1‑140 (MQ=255)
                                                                                                                                   cTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAGGATGtact       >  2:449476/1‑140 (MQ=255)
                                                                                                                                    tGGCAGATATGAGCGCCATGGTGGTAAAAGGGCAGGTATAAGAAGCGGATGTAATCCACGTGAAGGCGGGGCAATATGGGTGGGTTACGGTGCTTGGCGATCGACTGACGCGCTACGGGGGGGAAATCATGGATGtactg      >  2:1303347/1‑139 (MQ=255)
                                                                                                                                      gCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAGGATGTACTAcc    >  1:1398886/1‑140 (MQ=255)
                                                                                                                                        aGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAGGATGTACTACCGa  <  1:887108/140‑1 (MQ=255)
                                                                                                                                        aGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAGGATGTACTACCGa  <  1:115085/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CTCTCGATACGGCTAAAACCAATCTCGATTACACTCGCATCGTTGCCCCGATGGCCGGGGAAGTCACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAACAAGCACCGAACATTCTGACGCTGGCAGATATGAGCGCCATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGGCAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAGGATGTACTACCGA  >  NC_000913/919752‑920027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: