Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	992,071	C→A	6.0%	P484T (CCG→ACG)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	992,071	0	C	A	6.0%	373.5 / 5.6	100	P484T (CCG→ACG)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base C (40/54);  new base A (3/3);  total (43/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTACTTCCAGCCGGTGC  >  NC_000913/991941‑992209                                                                                                                                   |                                                                                                                                           cAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCa                                                                                                                                   <  2:330130/140‑1 (MQ=255)    ccAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCg                                                                                                                                <  1:885968/140‑1 (MQ=255)      aGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                                                                                                              <  1:813690/140‑1 (MQ=255)      aGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                                                                                                              <  1:498596/140‑1 (MQ=255)      aGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                                                                                                              <  1:1681278/140‑1 (MQ=255)      aGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                                                                                                              <  1:813635/140‑1 (MQ=255)      aGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                                                                                                              <  1:1210719/140‑1 (MQ=255)      aGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCAAGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                                                                                                              <  1:794310/140‑1 (MQ=255)       gTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAAc                                                                                                                             <  1:387351/140‑1 (MQ=255)       gTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAAc                                                                                                                             <  1:1276534/140‑1 (MQ=255)       gTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAAc                                                                                                                             <  1:1277050/140‑1 (MQ=255)        tCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACt                                                                                                                            >  2:1565328/1‑140 (MQ=255)        tCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACt                                                                                                                            >  2:1074399/1‑140 (MQ=255)             tACACCGATTGTGACCGTCATAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATg                                                                                                                       <  2:551887/140‑1 (MQ=255)             tACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCg                                                                                                                                >  2:77936/1‑131 (MQ=255)             tACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCg                                                                                                                                <  1:77936/131‑1 (MQ=255)             tACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATg                                                                                                                       >  2:685885/1‑140 (MQ=255)                 cagATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATaa                                                                                                                   <  1:1140183/138‑1 (MQ=255)                  cGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAAc                                                                                                                  >  2:157838/1‑140 (MQ=255)                  cGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAAc                                                                                                                  >  2:592896/1‑140 (MQ=255)                  cGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAAc                                                                                                                  >  2:592909/1‑140 (MQ=255)                  cGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAAc                                                                                                                  >  2:1034650/1‑140 (MQ=255)                  cGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAAc                                                                                                                  >  2:592936/1‑140 (MQ=255)                  cGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCAGCGAACTGTAGGATAAc                                                                                                                  >  1:225108/1‑140 (MQ=255)                   gATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACg                                                                                                                 <  1:1422217/140‑1 (MQ=255)                   gATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACg                                                                                                                 <  1:681612/140‑1 (MQ=255)                    aTTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGa                                                                                                                >  1:719450/1‑140 (MQ=255)                    aTTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGa                                                                                                                >  1:1438033/1‑140 (MQ=255)                       gtgACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAgg                                                                                                             <  2:1618100/140‑1 (MQ=255)                       gtgACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAgg                                                                                                             >  1:1496169/1‑140 (MQ=255)                       gtgACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAgg                                                                                                             <  2:1617850/140‑1 (MQ=255)                          aCCGTCAAAGACGCATACAATCCGGAAACCGAGCAGTACACCCTGACCATCAAACAGCTCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGCTCACGAAGGCaa                                                                                                          >  2:351713/1‑140 (MQ=255)                          aCCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCaa                                                                                                          >  1:267039/1‑140 (MQ=255)                          aCCGTCAAAGACGACTACAATCAGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGATGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCaa                                                                                                          >  2:1203654/1‑140 (MQ=255)                                  aGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATcc                                                                                                  <  2:688134/140‑1 (MQ=255)                                  aGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATcc                                                                                                  >  1:373431/1‑140 (MQ=255)                                       acTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTg                                                                                             >  2:1325427/1‑140 (MQ=255)                                              tCCGGAAACCGAGCAGTACACCCTGACCATCAGTCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAg                                                                                      >  1:579436/1‑140 (MQ=255)                                                 ggAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCg                                                                                   <  2:1340424/140‑1 (MQ=255)                                                 ggAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCg                                                                                   <  1:1618956/140‑1 (MQ=255)                                                  gAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCgg                                                                                  >  2:1422287/1‑140 (MQ=255)                                                  gAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCgg                                                                                  >  2:624391/1‑140 (MQ=255)                                                         aGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCg                                                                           >  1:570792/1‑140 (MQ=255)                                                                cacCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAAtt                                                                    <  1:1649425/140‑1 (MQ=255)                                                                   ccTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTACGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGAt                                                                                                    <  2:1355986/105‑1 (MQ=255)                                                                   ccTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTACGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGAt                                                                                                    >  1:71752/1‑105 (MQ=255)                                                                   ccTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTACGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGAt                                                                                                    >  1:71806/1‑105 (MQ=255)                                                                   ccTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTACGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGAt                                                                                                    <  2:71752/105‑1 (MQ=255)                                                                   ccTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTACGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGAt                                                                                                    >  1:1355986/1‑105 (MQ=255)                                                                   ccTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTACGATTGCCATCGAACTGTATGATAACGAAGGCAAAGTGAt                                                                                                    <  2:71806/105‑1 (MQ=255)                                                                     tGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTg                                                               >  1:728002/1‑140 (MQ=255)                                                                     tGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTg                                                               >  1:326075/1‑140 (MQ=255)                                                                      gACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGc                                                              >  1:1527773/1‑140 (MQ=255)                                                                      gACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGc                                                              >  1:1529754/1‑140 (MQ=255)                                                                      gACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGc                                                              >  1:1529763/1‑140 (MQ=255)                                                                       aCCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCt                                                             <  1:1466085/140‑1 (MQ=255)                                                                       aCCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCt                                                             <  1:153071/140‑1 (MQ=255)                                                                       aCCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCt                                                             <  1:1132246/140‑1 (MQ=255)                                                                       aCCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCt                                                             >  2:322685/1‑140 (MQ=255)                                                                       aCCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCt                                                             <  1:1637021/140‑1 (MQ=255)                                                                        ccATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTg                                                            >  1:887945/1‑140 (MQ=255)                                                                        ccATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTg                                                            >  1:887953/1‑140 (MQ=255)                                                                        ccATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTg                                                            >  1:780514/1‑140 (MQ=255)                                                                        ccATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTg                                                            >  1:1558121/1‑140 (MQ=255)                                                                        ccATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTg                                                            >  1:1124550/1‑140 (MQ=255)                                                                           tCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCg                                                                                                                                          >  2:784164/1‑59 (MQ=255)                                                                           tCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCg                                                                                                                                          <  1:784164/59‑1 (MQ=255)                                                                            cagccagcGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACg                                                        >  2:129573/1‑140 (MQ=255)                                                                             agccagcGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGt                                                       >  2:262367/1‑140 (MQ=255)                                                                               ccagcGCACGCCAGCCACCCCGGATCACGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTAGCAGAAAGGCGGTCAACCGGTGAATTCAGTGCTGAACGTCa                                                     >  2:1434082/1‑140 (MQ=255)                                                                                cagcGCACGCCAGCCACGCCGGGTCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCAc                                                    <  1:1151620/140‑1 (MQ=255)                                                                                cagcGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCAc                                                    <  1:1151581/140‑1 (MQ=255)                                                                                cagcGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCAc                                                    <  1:1151646/140‑1 (MQ=255)                                                                                     cACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAgg                                               <  2:1297786/140‑1 (MQ=255)                                                                                     cACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAgg                                               <  2:786325/140‑1 (MQ=255)                                                                                     cACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAgg                                               <  1:1591607/140‑1 (MQ=255)                                                                                     cACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAgg                                               <  1:287144/140‑1 (MQ=255)                                                                                     cACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAgg                                               <  2:162525/140‑1 (MQ=255)                                                                                        gccagccaCGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCgg                                            >  2:1497790/1‑140 (MQ=255)                                                                                         ccagccaCGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATcc                                                                                                  <  1:485004/85‑1 (MQ=255)                                                                                         ccagccaCGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATcc                                                                                                  >  2:485004/1‑85 (MQ=255)                                                                                          cagccaCGCCGGATCAGGCAGAAAACCAGCCGCTGCATATTCCGTTGGCCATCGAACTCTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGAGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGaa                                          <  2:199067/140‑1 (MQ=255)                                                                                          cagccaCGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGaa                                          <  1:1184941/140‑1 (MQ=255)                                                                                          cagccaCGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGaa                                          <  1:1185036/140‑1 (MQ=255)                                                                                            gccaCGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACa                                        <  1:1429188/140‑1 (MQ=255)                                                                                                cGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGAcc                                    >  1:37960/1‑140 (MQ=255)                                                                                                 gCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCt                                   <  2:429698/140‑1 (MQ=255)                                                                                                            cAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGAt                        <  1:1033494/140‑1 (MQ=255)                                                                                                            cAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGAt                        <  1:703364/140‑1 (MQ=255)                                                                                                             aGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATa                       <  1:979129/140‑1 (MQ=255)                                                                                                             aGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTAATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATa                       <  1:889850/140‑1 (MQ=255)                                                                                                             aGAAAAACAGCCGCTGCATATACCGTTTGCCAACGAACTGTATGATAACCAAGCCAAAGTCATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATACCGTGCTGAACGACACTCAGGCGGAACAGACCTTTGTCATTGATa                       <  2:729239/140‑1 (MQ=255)                                                                                                                 aaaCAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGt                   <  1:1478087/140‑1 (MQ=255)                                                                                                                  aaCAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTc                  <  2:559739/140‑1 (MQ=255)                                                                                                                      gCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTACt              <  2:693982/140‑1 (MQ=255)                                                                                                                             cATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGAGCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGACCGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTACTTCCAGcc       <  2:480315/140‑1 (MQ=255)                                                                                                                             cATAGTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTACTTCCAGcc       <  2:1331003/140‑1 (MQ=255)                                                                                                                                atTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTACTTCCAGCCGGt    <  2:1212862/140‑1 (MQ=255)                                                                                                                                atTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTACTTCCAGCCGGt    <  1:298816/140‑1 (MQ=255)                                                                                                                                  tCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTACTTCCAGCCGGTGc  <  1:188068/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                           CAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCCGGTGAATTCCGTGCTGAACGTCACTCAGGCGGAACAGACCTTTGTCTTTGATAATGTCTACTTCCAGCCGGTGC  >  NC_000913/991941‑992209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑