Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,028,117 C→A 5.5% Q58K (CAG→AAG)  yccU → putative CoA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,028,1170CA 5.5% 255.0 / 2.3 73Q58K (CAG→AAG) yccUputative CoA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (37/32);  new base A (2/2);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

CACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAACTCT  >  NC_000913/1027981‑1028238
                                                                                                                                        |                                                                                                                         
cACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGa                                                                                                                        <  1:1581454/140‑1 (MQ=255)
cACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGa                                                                                                                        <  1:106443/140‑1 (MQ=255)
cACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGa                                                                                                                        >  2:414823/1‑140 (MQ=255)
cACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGa                                                                                                                        <  1:1527423/140‑1 (MQ=255)
cACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGa                                                                                                                        <  1:1510447/140‑1 (MQ=255)
cACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGa                                                                                                                        <  1:1100762/140‑1 (MQ=255)
 aCTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGaa                                                                                                                       >  2:998677/1‑140 (MQ=255)
 aCTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGaa                                                                                                                       >  2:158970/1‑140 (MQ=255)
 aCTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGaa                                                                                                                       <  1:1564133/140‑1 (MQ=255)
 aCTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGaa                                                                                                                       >  2:996027/1‑140 (MQ=255)
       acCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTa                                                                                                                 >  2:83252/1‑140 (MQ=255)
       acCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTa                                                                                                                 >  2:83319/1‑140 (MQ=255)
           ttGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGc                                                                                                             >  1:360996/1‑140 (MQ=255)
           ttGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGc                                                                                                             >  1:361042/1‑140 (MQ=255)
           ttGCGCTGGTGGGGGCAAGCGACAAAACCGATCGTCCCAGCTATCGCTTGATGAAATATCTACTCGACGAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGc                                                                                                             >  2:1350287/1‑140 (MQ=255)
           ttGCGCGGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCCGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGc                                                                                                             >  2:608036/1‑140 (MQ=255)
            tGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGTCa                                                                                                            >  1:1652765/1‑140 (MQ=255)
             gcgcTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCAc                                                                                                           >  2:1476736/1‑140 (MQ=255)
              ctcTGGTGGGGGCACGCGACAAACCCGATCGTCCCAGCTATCGCGAGATGAAATCTCTACTCGACCAGGGGTCCCACCTCATCCCGGTTTCGCCAATATTGGCCGGCAAACCGCAGCTGGGCCATACTGGGTCTGGCACg                                                                                                          <  2:1412779/138‑1 (MQ=255)
                   gtggGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGc                                                                                                     >  2:1158569/1‑140 (MQ=255)
                   gtggGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGc                                                                                                     >  2:372365/1‑140 (MQ=255)
                   gtggGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGc                                                                                                     >  2:851098/1‑140 (MQ=255)
                   gtggGGGCAAGCGACAAACCCGATCGTCACAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCAAGAAGGGGTATGGCACGCTGGc                                                                                                     >  1:198266/1‑140 (MQ=255)
                   gtggGGGCAAGCGACAAACCCGATCGTCACAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCAAGAAGGGGTATGGCACGCTGGc                                                                                                     >  1:198261/1‑140 (MQ=255)
                          cAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGtt                                                                                              <  1:944344/140‑1 (MQ=255)
                                    aCCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAg                                                                                    <  1:490317/140‑1 (MQ=255)
                                        gATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGa                                                                                <  2:637887/140‑1 (MQ=255)
                                         aTCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGat                                                                               >  1:361798/1‑140 (MQ=255)
                                         aTCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGat                                                                               >  1:364566/1‑140 (MQ=255)
                                         aTCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGat                                                                               <  2:31236/140‑1 (MQ=255)
                                         aTCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCAGTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGat                                                                               <  2:1145891/140‑1 (MQ=255)
                                          tCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGata                                                                              <  2:511579/140‑1 (MQ=255)
                                          tCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGata                                                                              <  2:511572/140‑1 (MQ=255)
                                              cccAGCTATCGCGTGATGAAATATCGCCTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGACAAAGTCGATATGGt                                                                          <  2:666799/140‑1 (MQ=255)
                                               ccAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTc                                                                         >  1:1425545/1‑140 (MQ=255)
                                                cAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCg                                                                        >  1:549558/1‑140 (MQ=255)
                                                     aTCGCGTGATGACATATCTACTCGACCAGGGGAATCTCGTCATTGCGGCTTCGCCAAAAGTTGCCGGCAATACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCTGACGTACCGGTAAAAGTCGATAGGGTCGATGtt                                                                   <  2:482457/140‑1 (MQ=255)
                                                       cgcgTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGtttt                                                                 >  1:785869/1‑140 (MQ=255)
                                                       cgcgTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGtttt                                                                 >  1:69070/1‑140 (MQ=255)
                                                           tgatgaAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGt                                                             <  1:805024/140‑1 (MQ=255)
                                                                    atCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCAAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGaa                                                    <  2:198266/140‑1 (MQ=255)
                                                                    atCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCAAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGaa                                                    <  2:198261/140‑1 (MQ=255)
                                                                     tCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAg                                                   <  2:360996/140‑1 (MQ=255)
                                                                     tCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAg                                                   <  2:361042/140‑1 (MQ=255)
                                                                     tCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTAGATGTTTTCCGTAATTCAGAAg                                                   <  2:1309240/140‑1 (MQ=255)
                                                                     tCTACACGACCAGGGGTATCTCGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCATACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAg                                                   <  2:1674245/140‑1 (MQ=255)
                                                                            gACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTCTGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCggcgtg                                            >  1:185932/1‑140 (MQ=255)
                                                                            gACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCggcgtg                                            >  1:185874/1‑140 (MQ=255)
                                                                             aCCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCggcgtgg                                           >  1:597692/1‑140 (MQ=255)
                                                                              ccAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGGCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAATGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCggcgtggg                                          >  2:119321/1‑140 (MQ=255)
                                                                                       tCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGACAATGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGcac                                 >  1:201475/1‑140 (MQ=255)
                                                                                       tCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGcac                                 >  1:407065/1‑140 (MQ=255)
                                                                                        cACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGcaca                                <  2:613106/140‑1 (MQ=255)
                                                                                        cACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGcaca                                >  2:312167/1‑140 (MQ=255)
                                                                                        cACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTAAGAAGCGGCGTGGGGCGTGGcaca                                <  2:1526894/140‑1 (MQ=255)
                                                                                          cGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAgg                              <  1:414823/140‑1 (MQ=255)
                                                                                           gTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGa                             <  1:89376/140‑1 (MQ=255)
                                                                                           gTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGa                             <  1:1513704/140‑1 (MQ=255)
                                                                                           gTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGa                             <  2:1013131/140‑1 (MQ=255)
                                                                                            tCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGTTATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGaa                            >  2:1333783/1‑140 (MQ=255)
                                                                                            tCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGaa                            >  2:1336770/1‑140 (MQ=255)
                                                                                            tCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGaa                            >  1:1557072/1‑140 (MQ=255)
                                                                                            tCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGATTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGaa                            >  2:1141248/1‑140 (MQ=255)
                                                                                             cATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAg                           >  2:1505233/1‑140 (MQ=255)
                                                                                             cATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAg                           >  2:438539/1‑140 (MQ=255)
                                                                                             cATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAg                           >  2:797108/1‑140 (MQ=255)
                                                                                                    ctatCGACAAAAGTTGCCGGCAAAACGCTTATTGTCCATAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGc                    >  1:1398278/4‑140 (MQ=255)
                                                                                                     tttCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTAGTGcc                   >  1:1072611/1‑140 (MQ=255)
                                                                                                          ccAAAAGTTGCCGGGAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTgg              <  2:1336850/140‑1 (MQ=255)
                                                                                                                gTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGaa        <  2:1337982/140‑1 (MQ=255)
                                                                                                                  tGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGaaaa      <  2:1047084/140‑1 (MQ=255)
                                                                                                                  tGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGaaaa      <  2:389465/140‑1 (MQ=255)
                                                                                                                      ggCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAActct  <  1:1476736/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                         
CACTCACACCATTGCGCTGGTGGGGGCAAGCGACAAACCCGATCGTCCCAGCTATCGCGTGATGAAATATCTACTCGACCAGGGGTATCACGTCATTCCGGTTTCGCCAAAAGTTGCCGGCAAAACGCTGCTGGGCCAGAAGGGGTATGGCACGCTGGCAGACGTTCCGGAAAAAGTCGATATGGTCGATGTTTTCCGTAATTCAGAAGCGGCGTGGGGCGTGGCACAGGAAGCTATTGCCATTGGCGCGAAAACTCT  >  NC_000913/1027981‑1028238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: