Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 57,081 G→T 6.0% A10D (GCC→GAC)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091357,0810GT 6.0% 343.5 / 6.7 100A10D (GCC→GAC) lptDLPS assembly OM complex LptDE, beta‑barrel component
Reads supporting (aligned to +/- strand):  ref base G (50/44);  new base T (3/3);  total (53/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 105 total aligned reads displayed.

TTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAG  >  NC_000913/56946‑57218
                                                                                                                                       |                                                                                                                                         
ttGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAg                                                                                                                                       <  2:259255/140‑1 (MQ=255)
ttGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTGGGCACGCCCAACATGCTCTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAg                                                                                                                                       <  2:1821/140‑1 (MQ=255)
   aTAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGgag                                                                                                                                    >  2:98446/1‑140 (MQ=255)
   aTAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGgag                                                                                                                                    >  1:581732/1‑140 (MQ=255)
   aTAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGgag                                                                                                                                    >  1:581728/1‑140 (MQ=255)
     aGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGagag                                                                                                                                  <  2:1153894/140‑1 (MQ=255)
        cACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTgg                                                                                                                               >  1:1545306/1‑140 (MQ=255)
        cACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTgg                                                                                                                               >  1:1545692/1‑140 (MQ=255)
        cACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTgg                                                                                                                               >  2:649850/1‑140 (MQ=255)
        cACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTgg                                                                                                                               >  1:596847/1‑140 (MQ=255)
        cACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTgg                                                                                                                               >  1:1292330/1‑140 (MQ=255)
          cGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTgggg                                                                                                                             >  2:560398/1‑140 (MQ=255)
           gggTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGa                                                                                                                            >  2:1645317/1‑140 (MQ=255)
              tAAGTCACTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACTTTCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATAc                                                                                                                         <  2:932933/140‑1 (MQ=255)
                aGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGt                                                                                                                       >  2:596815/1‑140 (MQ=255)
                     ttGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGtttttt                                                                                                                  <  1:931159/140‑1 (MQ=255)
                     ttGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGtttttt                                                                                                                  <  1:931116/140‑1 (MQ=255)
                            cGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGt                                                                                                           >  1:494078/1‑140 (MQ=255)
                            cGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGt                                                                                                           >  1:812468/1‑140 (MQ=255)
                            cGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGt                                                                                                           >  1:226146/1‑140 (MQ=255)
                            cGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGt                                                                                                           >  1:1449572/1‑140 (MQ=255)
                             gCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGtt                                                                                                          >  1:433765/1‑140 (MQ=255)
                              cccAGTACCAGAGGACGGACATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCTGCTGCCAGTCCCAGTTGACTATAAAGGGCGGAGGCAATCAAGGAGGCCAGGAGAGAGGGGAAACGTTTTTTCATACGtag                                                                                                         >  2:1359942/1‑138 (MQ=255)
                               ccTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGa                                                                                                        <  2:1216829/140‑1 (MQ=255)
                                    aCCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTa                                                                                                   <  2:722279/140‑1 (MQ=255)
                                    aCCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTa                                                                                                   <  2:618110/140‑1 (MQ=255)
                                        gagGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGt                                                                                               >  2:235881/1‑140 (MQ=255)
                                         agGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGtt                                                                                              >  1:528152/1‑140 (MQ=255)
                                           gACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTcc                                                                                            <  1:1421735/140‑1 (MQ=255)
                                             cGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGTCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGa                                                                                                        >  1:167000/1‑126 (MQ=255)
                                             cGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGTCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGa                                                                                                        <  2:164015/126‑1 (MQ=255)
                                             cGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGTCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGa                                                                                                        >  1:166818/1‑126 (MQ=255)
                                             cGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGTCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGa                                                                                                        <  2:167000/126‑1 (MQ=255)
                                             cGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGTCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGa                                                                                                        <  2:166818/126‑1 (MQ=255)
                                             cGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGTCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGa                                                                                                        >  1:164015/1‑126 (MQ=255)
                                             cGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCcat                                                                                          >  1:976547/1‑140 (MQ=255)
                                             cGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCcat                                                                                          >  1:1027849/1‑140 (MQ=255)
                                             cGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCcat                                                                                          >  1:959247/1‑140 (MQ=255)
                                             cGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCcat                                                                                          >  1:1581033/1‑140 (MQ=255)
                                             cGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCcat                                                                                          >  1:645118/1‑140 (MQ=255)
                                             cGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCcat                                                                                          >  1:597059/1‑140 (MQ=255)
                                             cGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCAGTTGACTATAACGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCcat                                                                                          <  2:237751/140‑1 (MQ=255)
                                             cGGTCATAGAGTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCAAGTCCCTGTTGACTATAAAGGGAGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATAGGTTGATTTTATTGTTCcat                                                                                          <  2:237536/140‑1 (MQ=255)
                                                tCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCcatcat                                                                                       >  1:57737/1‑140 (MQ=255)
                                                tCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCcatcat                                                                                       >  2:543113/1‑140 (MQ=255)
                                                tCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGATTTTTCATACGTTGATTTTATTGTTCcatcat                                                                                       <  2:35857/140‑1 (MQ=255)
                                                        tGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACGATAAAGGGCGGTGGCAATCCTGGTGGCCCGGAGAGTGGGGATACGTGTGTTCATACGGTGATTTTATTGTTCCATCATCGGTAACg                                                                               >  2:755448/1‑140 (MQ=255)
                                                         ggCACGCCCAACGTGCACGGGGTGGCGCGGTCGGCTGCCAGTCCCTGTTGACTATCAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTGTTTTCATACGTTGATTTTATTGTTCCATCATCGGTATCGt                                                                              >  2:715669/1‑140 (MQ=255)
                                                         ggCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATTCGTTTGTTCATACGTTGATTTTATTGTTCCATCATCGGGAACGt                                                                              >  2:1186991/1‑140 (MQ=255)
                                                          gCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGtt                                                                             >  1:468939/1‑140 (MQ=255)
                                                              gCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGTGTGGGGATACGTTTTTTCATAAGGTGATTTTATTGTACCATCATCGGTAACGTTgcgc                                                                         >  2:1590652/1‑140 (MQ=255)
                                                                ccAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGt                                                                       >  2:1149096/1‑140 (MQ=255)
                                                                    cATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACa                                                                   <  1:1442769/140‑1 (MQ=255)
                                                                    cATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACa                                                                   <  1:1442761/140‑1 (MQ=255)
                                                                          cTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGt                                                             <  2:1229407/140‑1 (MQ=255)
                                                                          cTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGt                                                             <  2:1229586/140‑1 (MQ=255)
                                                                          cTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGt                                                             >  2:483953/1‑140 (MQ=255)
                                                                          cTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGt                                                             >  1:887238/1‑140 (MQ=255)
                                                                            gtgAGGCGAGGTCGTCTGCCAGTCCCTTTTGACTATAAAGGGAGGTGTCAATCATGGTGGCCAGTAGAGTGGGGATAATTTTTTTCATAAGTTGAATTTATTGTTCAATCATCGGTAACGTTGCGCGTGACAACCGGACa                                                           <  2:1622576/140‑1 (MQ=255)
                                                                             tgAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCag                                                          >  1:326875/1‑140 (MQ=255)
                                                                             tgAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCag                                                          >  1:924964/1‑140 (MQ=255)
                                                                              gAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCaga                                                         >  2:921099/1‑140 (MQ=255)
                                                                               aGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGTTAGTGGGGATACGTTTGTTCTTACGGTGAGTTGATTGTTCCTTCATCGGTAACGTTGCGCGTGACATTCGGTCagag                                                        >  2:1665552/1‑140 (MQ=255)
                                                                               aGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCagag                                                        >  1:171309/1‑140 (MQ=255)
                                                                                     ggTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGagag                                                                                                                                  <  1:1563617/60‑1 (MQ=255)
                                                                                     ggTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGagag                                                                                                                                  >  2:1563617/1‑60 (MQ=255)
                                                                                       tCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGGGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGt                                                >  2:1611473/1‑140 (MQ=255)
                                                                                        cGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTa                                               <  2:468939/140‑1 (MQ=255)
                                                                                                 gTCCCTGTTGACTATAAAGGGCGGTGGCAAACATGGTGGCCAGGAGAGTGGCGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGAACTCGTCAtc                                      >  2:139415/1‑140 (MQ=255)
                                                                                                  tCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGTGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCAtct                                     <  2:876651/140‑1 (MQ=255)
                                                                                                  tCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCAtct                                     <  2:700162/140‑1 (MQ=255)
                                                                                                  tCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCAtct                                     <  2:1289162/140‑1 (MQ=255)
                                                                                                  tCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCAtct                                     <  2:170086/140‑1 (MQ=255)
                                                                                                  tCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGGTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCAtct                                     <  2:1368187/140‑1 (MQ=255)
                                                                                                    ccTGTTGACTATAAAGGGCGGTGGCAATAATGGAGGCCAGGAGAGTGGGGATAAGTTATTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCAtctca                                   >  2:585191/1‑139 (MQ=255)
                                                                                                        ttGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGc                               <  1:317464/140‑1 (MQ=255)
                                                                                                        ttGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGc                               <  1:1109594/140‑1 (MQ=255)
                                                                                                        ttGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGc                               <  2:1083705/140‑1 (MQ=255)
                                                                                                         tGACTATATAGGGCTGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTACCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTAATCTCTACGCt                              <  2:244547/140‑1 (MQ=255)
                                                                                                         tGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGTGTGGGGATACGTTTTTTCATACTTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTTCTCGTCATCTCTCCGCt                              <  2:244528/140‑1 (MQ=255)
                                                                                                         tGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCt                              <  1:1598865/140‑1 (MQ=255)
                                                                                                         tGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCt                              <  2:962993/140‑1 (MQ=255)
                                                                                                         tGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCt                              <  1:1168027/140‑1 (MQ=255)
                                                                                                         tGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCt                              <  1:923577/140‑1 (MQ=255)
                                                                                                         tGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCt                              <  1:689492/140‑1 (MQ=255)
                                                                                                         tGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCt                              <  2:663796/140‑1 (MQ=255)
                                                                                                         tGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCt                              <  1:361834/140‑1 (MQ=255)
                                                                                                         tGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCt                              <  1:689477/140‑1 (MQ=255)
                                                                                                         tGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCt                              <  2:555074/140‑1 (MQ=255)
                                                                                                         tGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCt                              <  2:502529/140‑1 (MQ=255)
                                                                                                         tGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCt                              <  1:595203/140‑1 (MQ=255)
                                                                                                         cgACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCt                              <  1:1682331/139‑1 (MQ=255)
                                                                                                             tataAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATAAGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAgtg                          >  1:21863/1‑140 (MQ=255)
                                                                                                               taAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGtt                        >  2:1381464/1‑140 (MQ=255)
                                                                                                                aaaGGGCGGTGGCAAACATGGTGGCCAGGACAGGGGGGATACGTTTTTTCATACCTTGAAATAATTGTACCAACATCGGACACGTTGCGCGAGACAAACGGTCAGAGACTCACGACCTCGTCAACTCTACGCTCGTGATa                       >  2:1662452/1‑140 (MQ=255)
                                                                                                                       ggTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGt                >  1:622129/1‑140 (MQ=255)
                                                                                                                                  tggtggCCATGAGAGTGTGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTTCGCGTGACAAACTGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGt     <  2:1434313/140‑1 (MQ=255)
                                                                                                                                     tggCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTTACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAg  <  1:98446/140‑1 (MQ=255)
                                                                                                                                     tggCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAg  <  1:618970/140‑1 (MQ=255)
                                                                                                                                     tggCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAg  <  2:1601654/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                         
TTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGAGGCGAGGTCGGCTGCCAGTCCCTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAG  >  NC_000913/56946‑57218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: