BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,049,827	T→G	7.5%	intergenic (‑83/‑6)	gfcA ← / → insA	O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,049,827	0	T	G	7.5%	278.4 / 14.7	107	intergenic (‑83/‑6)	gfcA/insA	O‑antigen capsule production threonine‑rich inner membrane protein/IS1 repressor TnpA
Reads supporting (aligned to +/- strand): ref base T (58/41); new base G (6/2); total (64/43)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

Only 100 of 110 total aligned reads displayed.

CGCAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTA  >  NC_000913/1049688‑1049966

cGCAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGtt                                                                                                                                             <  1:544096/140‑1 (MQ=255)

  cAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGGtct                                                                                                                                           >  2:1482911/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:1648996/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  1:700961/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:1010974/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:1018661/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:104800/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:1325332/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:45526/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:469052/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  1:1224429/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  1:1224393/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  2:469061/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  1:337914/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCt                                                                                                                                  >  2:937720/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCt                                                                                                                                  >  2:1059357/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCt                                                                                                                                  >  2:881633/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCt                                                                                                                                  >  2:881233/1‑140 (MQ=255)

           tcgtcAACATGAAGGCCATAAGGAATGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATGTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCt                                                                                                                                  >  1:245502/1‑140 (MQ=255)

           acaacAAAATGAACGACATAAGGATTGCAGAAAGATTTTTATACAAAACTATTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCt                                                                                                                                  >  1:1591388/5‑140 (MQ=18)

            cgtcAACTTGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATATCTTTTCCTTTATTCATCGCTTGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCtt                                                                                                                                 <  2:881943/140‑1 (MQ=255)

            cgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCtt                                                                                                                                 <  2:597020/140‑1 (MQ=255)

            cgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCtt                                                                                                                                 >  1:873817/1‑140 (MQ=255)

            cgtcAACATGAAGGCCATAAGGAGAGCAGAAAGCTTGTGTTTCTTAACTTTTCCTTTATTCATCGCATGGTCAATACGGGTGATGCTGCCAACTTATTGATTTAGTGTATGATGGTGTTTTTGAGGTTGTCCAGTGGCtt                                                                                                                                 <  2:882026/140‑1 (MQ=37)

               cAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTg                                                                                                                              <  2:876601/140‑1 (MQ=255)

               cAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTg                                                                                                                              <  1:1506640/140‑1 (MQ=255)

                   aagAAGGCCATAACGATTGCAGTCAGCTTGTGTTTCATAACTATTCCTATATTCATCGCATGGACAATACTGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTACTGTTTc                                                                                                                          <  1:1653466/138‑1 (MQ=25)

                        ggCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCa                                                                                                                     <  2:837203/140‑1 (MQ=255)

                          ccATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGc                                                                                                                   <  2:1542514/140‑1 (MQ=255)

                             tAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGt                                                                                                                <  1:1297469/140‑1 (MQ=255)

                             tAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCAAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGt                                                                                                                >  2:767078/1‑140 (MQ=255)

                                   ttGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCcctcc                                                                                                          >  1:1444495/1‑140 (MQ=255)

                                   ttGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGGTTCTATCAGCTGTCcctcc                                                                                                          >  2:16578/1‑140 (MQ=255)

                                    tGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCcctcct                                                                                                         >  2:312959/1‑140 (MQ=255)

                                    tGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCcctcct                                                                                                         >  2:1131499/1‑140 (MQ=255)

                                    tGCAGAAAGCTTGTGTTGCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCcctcct                                                                                                         >  1:81158/1‑140 (MQ=255)

                                           aGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGc                                                                                                  >  1:1638011/1‑140 (MQ=255)

                                           aGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGc                                                                                                  >  1:455978/1‑140 (MQ=255)

                                           aGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGc                                                                                                  >  1:453880/1‑140 (MQ=255)

                                           aGCTTGTGTTTCAGAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGc                                                                                                  >  1:438619/1‑140 (MQ=255)

                                              ttGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACATTACGGGTGTTGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTAc                                                                                               <  2:1423024/140‑1 (MQ=255)

                                              ttGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTAc                                                                                               <  2:1354577/140‑1 (MQ=255)

                                              ttGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTAc                                                                                               <  1:45526/140‑1 (MQ=255)

                                              ttGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTAc                                                                                               <  2:1324584/140‑1 (MQ=255)

                                              ttGTGTATCATAACGTGTCCTTTATTCACCGCATGGACAAACCGGGTGATGCTGCCAACTTACAGATTTAGTGTATGATGGTGATTTTGAGGGTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTAc                                                                                               <  2:1673173/140‑1 (MQ=17)

                                                gtgtTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTg                                                                                             >  2:1556876/1‑140 (MQ=255)

                                                gtgtTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTg                                                                                             >  2:405447/1‑140 (MQ=255)

                                                   tttCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACg                                                                                          <  1:644539/140‑1 (MQ=255)

                                                   tttCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACg                                                                                          <  1:643777/140‑1 (MQ=255)

                                                   tttCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGAATTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACg                                                                                          <  1:194678/140‑1 (MQ=255)

                                                   tttCATAACTTTTCCTTTATACATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACg                                                                                          <  1:194833/140‑1 (MQ=255)

                                                      cATAACTTTTCCTTTATTCATCGCCTGGACTATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACgggg                                                                                       <  2:1630744/140‑1 (MQ=255)

                                                       aTAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGggt                                                                                      >  2:413203/1‑140 (MQ=255)

                                                        tAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGggtg                                                                                     <  2:680589/140‑1 (MQ=255)

                                                             tttCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCg                                                                                >  2:719230/1‑140 (MQ=255)

                                                              ttCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGt                                                                               >  1:1251299/1‑140 (MQ=255)

                                                               tCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTa                                                                              <  2:1591388/140‑1 (MQ=255)

                                                                 cTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAAc                                                                            <  1:1059357/140‑1 (MQ=255)

                                                                      ttCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCaa                                                                       >  2:22295/1‑140 (MQ=255)

                                                                      atCATCGAATCCACAATACGGGTGATGCTGCCAACTTACAGATTTAGTGTATGATGGTGTTTTTGAGTTTCTCCAGTGGCTTCTGTTTAGATCAGCTGTCCCTCCTGTTCAGCAACTGACGGGGTGGTTCGTAACGGCat                                                                       >  1:448654/2‑139 (MQ=14)

                                                                           cGCATGGACAATACGTGAGATGCTGCACACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTCGCTACTGGTTCTGTCAGCTTTCCCTCCTGTTCAGCTACTGACGGGGAGGTGCGTAACTGCAAAAGCa                                                                  >  2:847838/1‑140 (MQ=14)

                                                                           cGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCATCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGAAAAAGCa                                                                  >  2:1369406/1‑140 (MQ=255)

                                                                           cGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCa                                                                  >  1:23218/1‑140 (MQ=255)

                                                                           cGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCa                                                                  >  1:99837/1‑140 (MQ=255)

                                                                           cGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCa                                                                  >  1:1140216/1‑140 (MQ=255)

                                                                           cGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCa                                                                  >  2:1283029/1‑140 (MQ=255)

                                                                              aTGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTg                                                               <  2:700961/140‑1 (MQ=255)

                                                                                 gACAATTCGGGTTATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCAGTGCCg                                                            <  1:933575/140‑1 (MQ=255)

                                                                                 gACAATTCGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCg                                                            <  1:933390/140‑1 (MQ=37)

                                                                                     atttcggTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACa                                                        <  1:1308784/135‑1 (MQ=0)

                                                                                         cggTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAg                                                    >  1:623853/2‑140 (MQ=14)

                                                                                           gTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAgcg                                                  >  1:325094/1‑140 (MQ=32)

                                                                                            tGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAgcgc                                                 <  2:1465843/140‑1 (MQ=32)

                                                                                            tGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGATATTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAgcgc                                                 <  2:1300487/140‑1 (MQ=17)

                                                                                             gATGCTGCCAACTAACTGATTTAGTGTATGATGGAGATTTAGAGCTTCTCCAGTGGCTACAGATTCTAACAGCTGACCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCt                                                <  2:491991/140‑1 (MQ=11)

                                                                                                 ctgcCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTAtct                                            <  2:380371/140‑1 (MQ=32)

                                                                                                        cttacttaTTTTGTGTATGTTTGTGTTTTTGAGGTTCTCCAGTGGATTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGTTGGTGCGTATCGGCAAAAGCACTGCCGGACATCTGCGCTATCTCTGctct                                     <  1:1676383/140‑1 (MQ=11)

                                                                                                           aCTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCAc                                  <  1:1407017/140‑1 (MQ=32)

                                                                                                           aCTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCAc                                  <  1:1458765/140‑1 (MQ=32)

                                                                                                           aCTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCAc                                  <  1:1022871/140‑1 (MQ=32)

                                                                                                             tGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTg                                <  2:777354/140‑1 (MQ=32)

                                                                                                                tttAGTGTATGATGGTGTTTTTGTGGTTCTCAAGTGGCTTCTGTTTCTATCAGCTGTCCATCATGTTAAGCTTCTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCg                             <  2:1603748/140‑1 (MQ=11)

                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCg                                                            >  2:371283/1‑106 (MQ=32)

                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCg                                                            <  1:371283/106‑1 (MQ=32)

                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  2:968309/1‑140 (MQ=32)

                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  2:500421/1‑140 (MQ=32)

                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  2:391872/1‑140 (MQ=32)

                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  2:753273/1‑140 (MQ=32)

                                                                                                                   aGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaa                          >  1:1521658/1‑140 (MQ=32)

                                                                                                                     tgtATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaaaa                        >  1:269106/1‑140 (MQ=32)

                                                                                                                     ggtATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATAATCTGTCCCTCCTGTTAAGCTACTTACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTaaaa                        >  1:164696/2‑140 (MQ=255)

                                                                                                                               tgtTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACt              >  2:1258266/1‑140 (MQ=32)

                                                                                                                               tgtTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACt              >  1:1021742/1‑140 (MQ=32)

                                                                                                                               tgtTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACAGCCGTAAAACAAGGCAACt              >  1:1190652/1‑140 (MQ=17)

                                                                                                                                 tttttGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGc            <  2:873535/140‑1 (MQ=32)

                                                                                                                                 tttttGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGc            <  2:81633/140‑1 (MQ=32)

                                                                                                                                   tttGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTTCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACAAGGCAACTGCAg          >  1:207035/1‑140 (MQ=17)

                                                                                                                                     tGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGtt        <  2:865131/140‑1 (MQ=32)

                                                                                                                                        ggTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCAc     >  2:968681/1‑140 (MQ=32)

                                                                                                                                           tctcCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTa  >  1:506886/1‑140 (MQ=11)

CGCAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTA  >  NC_000913/1049688‑1049966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`