| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,227,332 | G→A | 12.0% | Y47Y (TAC→TAT) | pliG ← | periplasmic inhibitor of g‑type lysozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,227,332 | 0 | G | A | 12.0% | 145.8 / 10.8 | 50 | Y47Y (TAC→TAT) | pliG | periplasmic inhibitor of g‑type lysozyme |
| Reads supporting (aligned to +/- strand): ref base G (22/22); new base A (3/3); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
AGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTT > NC_000913/1227195‑1227469 | aGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTatcgtaatcgta < 2:1435586/140‑1 (MQ=255)aGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTatcgtaatcgta < 2:114076/140‑1 (MQ=255) atatCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTAttt < 1:893872/140‑1 (MQ=255) cTGGATAATTCAACTGAATCATCGATTCGTGGCCCGAAGAGGTAAGTATCAGCGCGTTCATTAGAGATACTCTCATGCACTTTCTGTCGTTTTTTGGCATAGTAGGTATATGTATCGTAATCGTATCCCTTTATTTCGcc > 2:441226/1‑140 (MQ=255) tGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCg < 1:1212213/140‑1 (MQ=255) tAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGt > 2:558133/1‑140 (MQ=255) tAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGt > 2:558117/1‑140 (MQ=255) tAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGt > 2:557473/1‑140 (MQ=255) tAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCACTTTATTTCGCCGGAGt > 2:940871/1‑140 (MQ=255) aaCTGAATCATCGATTCCTGGCCCGAAGAGGTACGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCATTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAg < 2:65514/140‑1 (MQ=255) aaCTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTATTCGTATCCCTTTATTTCGCCGGAGTACTGAg < 1:1501709/140‑1 (MQ=255) aaCTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAg < 1:405392/140‑1 (MQ=255) aaCTGAATCATCGAGTCCTGGCCCGAAGAGGTAAGTATCAGCGCCGTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAg < 2:529271/140‑1 (MQ=255) cTGAATCATCGATTCCTGGCCCGCAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAgcg < 2:959223/140‑1 (MQ=255) cTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAgcg < 1:783322/140‑1 (MQ=255) tcGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATg > 2:326494/1‑140 (MQ=255) aGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGa < 1:634554/140‑1 (MQ=255) tATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACa < 1:1017188/140‑1 (MQ=255) gcgcCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCtt > 2:1194735/1‑140 (MQ=255) gcgcCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCtt > 2:1207804/1‑140 (MQ=255) gcgcCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCtt > 2:1194796/1‑140 (MQ=255) ccTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTAcc < 2:349072/140‑1 (MQ=255) ttCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCg > 2:514997/1‑140 (MQ=255) ttCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCg < 2:269870/140‑1 (MQ=255) ttCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCg < 2:269755/140‑1 (MQ=255) ttCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCg > 2:141909/1‑140 (MQ=255) agagATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGCATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCaa > 1:247911/1‑140 (MQ=255) aTACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGATTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACAATGACATTCTTACCCGCAGCAAAGcg > 2:1291711/1‑139 (MQ=255) aTACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCt > 2:129022/1‑140 (MQ=255) aCTCACATGCACTTTCTGACCTTTTTTGGCATTGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCg > 2:834769/1‑140 (MQ=255) aCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCg < 1:1019670/140‑1 (MQ=255) aCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCg > 1:1335638/1‑140 (MQ=255) aCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCg < 1:1454592/140‑1 (MQ=255) aCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCg < 1:1456675/140‑1 (MQ=255) aCTCACATGCACTTTATGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCg < 2:264497/140‑1 (MQ=255) caTGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAg < 2:747751/140‑1 (MQ=255) aTGCACTTTCTGAGCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGtggtgg > 1:480344/1‑140 (MQ=255) aTGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAgg > 1:1500690/1‑140 (MQ=255) cACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTa > 2:41145/1‑140 (MQ=255) tCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGt > 1:1148007/1‑140 (MQ=255) ccTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAag < 1:362545/140‑1 (MQ=255) tttttGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACACGCAGCAAAGATCTTGGAGGTTAGCAGTGCAagcag > 2:750997/1‑140 (MQ=255) ggCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATa < 1:81640/140‑1 (MQ=255) gAAGGTATATGTATCGTAATCATATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCg > 1:673501/1‑107 (MQ=255) gAAGGTATATGTATCGTAATCATATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCg > 1:673502/1‑107 (MQ=255) gAAGGTATATGTATCGTAATCATATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCg < 2:673529/107‑1 (MQ=255) gAAGGTATATGTATCGTAATCATATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCg < 2:673502/107‑1 (MQ=255) gAAGGTATATGTATCGTAATCATATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCg < 2:673501/107‑1 (MQ=255) gAAGGTATATGTATCGTAATCATATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCg > 1:673529/1‑107 (MQ=255) tataTGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTg < 2:492955/140‑1 (MQ=255) atatGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGATATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGa < 1:441226/140‑1 (MQ=255) tATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGctc > 1:1100302/1‑140 (MQ=255) cgtaatcgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGAGATTCTTACCCGCAGCAAAGCTCGTGGAGGTTTGCTGTGCAAGCAGCTATACAGCCTTCCTGATGGTGTTg > 1:1442024/1‑140 (MQ=255) cgtaatcgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTg > 1:1139668/1‑140 (MQ=255) cgtaatcgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTg > 2:766493/1‑140 (MQ=255) cgtaatcgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTg > 2:217603/1‑140 (MQ=255) cgtaatcgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGAAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTg > 1:1139658/1‑140 (MQ=255) gtaatcgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTgt > 1:832943/1‑139 (MQ=255) gtaatcgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGa > 1:784403/1‑140 (MQ=255) gtaatcgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGa > 1:1033997/1‑140 (MQ=255) gtaatcgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGa > 1:392697/1‑140 (MQ=255) tcgtaTCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGAtttt > 2:244147/1‑140 (MQ=255) | AGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTT > NC_000913/1227195‑1227469 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |