Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,270,543 T→A 5.8% intergenic (+83/+206) rdlC → / ← chaA sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,270,5430TA 5.8% 349.1 / 6.5 117intergenic (+83/+206)rdlC/chaAsRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (61/48);  new base A (6/2);  total (67/50)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

AGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTA  >  NC_000913/1270404‑1270679
                                                                                                                                            |                                                                                                                                        
aGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGt                                                                                                                                          <  1:780717/140‑1 (MQ=40)
           cGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATc                                                                                                                               >  1:1075950/1‑140 (MQ=255)
                tgttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCACCCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCGTGGTGTGGATACAACGTCGCAATTCATcatcac                                                                                                                          >  2:1597165/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCATCGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:1214471/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATTCTACGTCGCAATTCATcttcact                                                                                                                         >  2:1040326/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCATTTCATcttgact                                                                                                                         >  2:1665050/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:726349/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:192186/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:577764/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:726503/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:840902/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:1217797/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:194698/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:53679/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:362317/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:857735/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:1313967/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:1554500/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                         >  2:336635/1‑140 (MQ=255)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATcttcact                                                                                                                         >  2:642689/1‑140 (MQ=14)
                 gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATcttcact                                                                                                                         >  1:432582/1‑140 (MQ=14)
                  ttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcactt                                                                                                                        >  1:172940/1‑140 (MQ=255)
                     tCAGGTTTTACCTATCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACAGTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCAt                                                                                                                     >  2:1005803/1‑140 (MQ=255)
                     tCAGGGTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGCTAACGCCGGCTTGGTGTGGATACGACGTCGCACATCATCTTCACTTCAt                                                                                                                     >  2:632197/1‑140 (MQ=14)
                       aGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATcc                                                                                                                   >  1:329089/1‑140 (MQ=255)
                         gTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCt                                                                                                                 <  2:358208/140‑1 (MQ=255)
                          ttttACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTg                                                                                                                >  2:1639216/1‑140 (MQ=255)
                                   tcAACGTGCGGGTGTTTTCTCTTTCCAGCAACCAATGCCACCATGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTg                                                                                                       >  1:1619580/1‑140 (MQ=255)
                                   tcAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTg                                                                                                       >  1:727739/1‑140 (MQ=39)
                                     aaCGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCa                                                                                                     <  1:289940/140‑1 (MQ=39)
                                     aaCGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCa                                                                                                     <  2:1252091/140‑1 (MQ=39)
                                     aaCGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCa                                                                                                     <  1:1639216/140‑1 (MQ=39)
                                          gCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGaa                                                                                                >  2:1365114/1‑140 (MQ=39)
                                           cGGGGGTTTTGTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGaa                                                                                                <  2:557741/140‑1 (MQ=0)
                                           cGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGaa                                                                                                >  2:980713/1‑140 (MQ=0)
                                             ggggTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag                                                                                             <  2:359981/140‑1 (MQ=39)
                                             ggggTGTTCTCGTGCCCGCACCCATTGCCACCCGGGATAACGCCCCGCAACAGTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag                                                                                             <  2:1049082/140‑1 (MQ=14)
                                                          tCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACTCCGGCATGGTGGGGATACTACGTCGCAATTCATCTTCAAATCATCCCTGTAATGTTTGCAATGAAGAGTACCTTCCGGCtt                                                                                >  2:1043336/1‑140 (MQ=14)
                                                          tCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGa                                                                                                 >  2:980919/1‑123 (MQ=40)
                                                          tCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGa                                                                                                 <  1:980919/123‑1 (MQ=40)
                                                                aaCCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAAc                                                                          >  2:304862/1‑140 (MQ=255)
                                                                aaCCAAGGCCACACGGGTTAATGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAAc                                                                          <  1:280238/140‑1 (MQ=14)
                                                                 aCCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAAt                                                                                                   <  2:448244/114‑1 (MQ=40)
                                                                 aCCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAAt                                                                                                   >  1:448244/1‑114 (MQ=40)
                                                                  ccAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAg                                                                        >  1:847970/1‑140 (MQ=255)
                                                                          accaGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCa                                                                <  1:707645/140‑1 (MQ=255)
                                                                            caGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGt                                                              >  1:912510/1‑140 (MQ=255)
                                                                             tgggATAACGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTg                                                             <  1:1179643/139‑1 (MQ=255)
                                                                             aGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATACCGGCTTTTCAACAGCTGTTGCAGTg                                                             <  2:1330598/140‑1 (MQ=255)
                                                                              gggATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTgg                                                            >  2:31863/1‑140 (MQ=255)
                                                                                  tAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTc                                                        >  1:242040/1‑140 (MQ=255)
                                                                                     aGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTg                                                     <  1:1342998/140‑1 (MQ=255)
                                                                                     aGCACCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCAGCTTCAATTCATCCCTGACTGGTTTGCAGTGACGAGGTCAGTCCGGCGTTTCCGCAGCTGGTGCGGTGGTTTCggg                                                     >  2:266179/1‑138 (MQ=14)
                                                                                        ccccGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCa                                                   <  1:738426/140‑1 (MQ=255)
                                                                                        ccccGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCa                                                   <  1:518291/140‑1 (MQ=255)
                                                                                        ccccGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCa                                                   <  2:313062/140‑1 (MQ=255)
                                                                                        ccccGCAACATTGCGCCTCACCGGATAACGCCGGCATGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCa                                                   <  2:899471/140‑1 (MQ=255)
                                                                                          ccGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTg                                                     <  1:1343077/136‑1 (MQ=21)
                                                                                          ccGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTg                                                     >  2:1343077/1‑136 (MQ=21)
                                                                                          ccGCAACATTGCGCCTCACCGGATAACTCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTg                                                     <  1:1343111/136‑1 (MQ=255)
                                                                                           cGCAACATTGCGCCTCACCGGATAACGCCTGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAggg                                                >  2:788847/1‑140 (MQ=255)
                                                                                           cGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATAGTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGTGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAggg                                                >  2:1195097/1‑140 (MQ=255)
                                                                                           cGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAGATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAggg                                                >  1:98556/1‑140 (MQ=255)
                                                                                           cGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCACTGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAggg                                                >  2:1087284/1‑140 (MQ=255)
                                                                                           cGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAggg                                                >  2:1238282/1‑140 (MQ=255)
                                                                                           cGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAggg                                                >  1:301965/1‑140 (MQ=255)
                                                                                             ctaCATTGCGCCTCACCGGATAACTCCGGCTTGGTGTGGATACTAAGTCGCTATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGATGTGTTCATTCCGGGTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGgtg                                              <  2:1654366/138‑1 (MQ=14)
                                                                                                  ttGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTa                                         >  2:1621935/1‑140 (MQ=255)
                                                                                                  ttGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTa                                         >  1:1211194/1‑140 (MQ=255)
                                                                                                  ttGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACAACTTCGCGACTCTTCTTCACTTCGTCGCTGTGTTGTTTGCAATGAGGTGTACATTACGGGTTTTAATAAGCTGTTGGTGTTGTTTGGGGAAGGGggtggtt                                         >  2:1626125/1‑139 (MQ=9)
                                                                                                       ccTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCGTGCAGGGTGTGGTACAGGc                                    >  2:931968/1‑140 (MQ=255)
                                                                                                        cTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCt                                   >  1:210003/1‑140 (MQ=255)
                                                                                                                 tAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAATTTTTTGCAATGATTAGTAATTTCCGGCTTTTCTACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCt                          <  1:886432/140‑1 (MQ=21)
                                                                                                                 tAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCt                          <  1:726349/140‑1 (MQ=255)
                                                                                                                  aaCGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTg                         >  1:794924/1‑140 (MQ=255)
                                                                                                                  aaCGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTg                         >  1:636930/1‑140 (MQ=255)
                                                                                                                    cGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGAt                       >  1:793047/1‑140 (MQ=255)
                                                                                                                         gCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACg                  >  1:944470/1‑140 (MQ=255)
                                                                                                                         gCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGGTTTTCAACAGCTGTTGCAGTCTTTTCGTGATGGATGTGGTAGAGGCTACCAACTCTGATTAAct                  >  1:961570/1‑139 (MQ=14)
                                                                                                                         gCTTGGTGTGGATACTACGACGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCAGGATTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCACGCAAATCTGATTAACg                  >  1:961263/1‑140 (MQ=18)
                                                                                                                          cTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGa                 <  2:207415/140‑1 (MQ=255)
                                                                                                                           ttGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGAc                <  2:1615409/140‑1 (MQ=255)
                                                                                                                           ttGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAACTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGAc                <  1:548258/140‑1 (MQ=255)
                                                                                                                           ttGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGAc                <  1:192186/140‑1 (MQ=255)
                                                                                                                           ttGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGAc                <  1:1021462/140‑1 (MQ=255)
                                                                                                                           ttGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGAc                <  2:288514/140‑1 (MQ=255)
                                                                                                                           ttGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGAc                <  2:1438808/140‑1 (MQ=255)
                                                                                                                            tGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGa                                                                                                 <  1:1547487/58‑1 (MQ=40)
                                                                                                                            tGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGAcc               <  1:1678232/140‑1 (MQ=255)
                                                                                                                              gtgtGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAAc                                                                          <  2:1567445/79‑1 (MQ=39)
                                                                                                                                gtgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTc           <  1:980713/140‑1 (MQ=255)
                                                                                                                                   gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCa                                                   <  2:587366/97‑1 (MQ=255)
                                                                                                                                   gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCa                                                   >  1:587366/1‑97 (MQ=255)
                                                                                                                                    aTACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAgg       <  1:1300909/140‑1 (MQ=255)
                                                                                                                                    aTACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAgg       <  1:1234072/140‑1 (MQ=255)
                                                                                                                                    aTACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAgg       <  2:1686695/140‑1 (MQ=255)
                                                                                                                                    aTACGACGTCGCAATACATCAACACTTCAACCCTGAAATGTTTGCAATGAAGAGTACCTTCCGGCTTATCAACCGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAgg       <  2:1686849/140‑1 (MQ=37)
                                                                                                                                        tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCTGgtagt   >  1:670081/1‑140 (MQ=255)
                                                                                                                                        tacGTCGCAATTCATCTTCAATTCATCGCTGAAATGTTTGAAATGAAGAGTAAATTCCGGGTTTTCAACCGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACTGGCTCGCAATTCTTATTAACTACCTGTCCAGgtagt   >  1:850751/1‑140 (MQ=255)
                                                                                                                                         acGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGgtagta  <  1:879124/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                        
AGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTA  >  NC_000913/1270404‑1270679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: