Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,270,545 G→T 6.5% intergenic (+85/+204) rdlC → / ← chaA sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,270,5450GT 6.5% 387.3 / 8.0 121intergenic (+85/+204)rdlC/chaAsRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (66/46);  new base T (5/4);  total (71/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

TTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAG  >  NC_000913/1270407‑1270684
                                                                                                                                           |                                                                                                                                           
ttAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGc                                                                                                                                            <  2:453818/140‑1 (MQ=255)
        cGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATc                                                                                                                                    >  1:1075950/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCATCGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                              >  2:1214471/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATTCTACGTCGCAATTCATcttcact                                                                                                                              >  2:1040326/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCATTTCATcttgact                                                                                                                              >  2:1665050/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                              >  2:1217797/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                              >  2:192186/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                              >  2:194698/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                              >  2:335405/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                              >  2:1554500/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                              >  2:1313967/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                              >  2:362317/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                              >  2:577764/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                              >  2:726349/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                              >  2:726503/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                              >  2:840902/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact                                                                                                                              >  2:857588/1‑140 (MQ=255)
              gttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATcttcact                                                                                                                              >  1:432582/1‑140 (MQ=14)
               ttgtCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcactt                                                                                                                             >  1:172940/1‑140 (MQ=255)
                  tCAGGTTTTACCTATCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACAGTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCAt                                                                                                                          >  2:1005803/1‑140 (MQ=255)
                  tCAGGGTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGCTAACGCCGGCTTGGTGTGGATACGACGTCGCACATCATCTTCACTTCAt                                                                                                                          >  2:632197/1‑140 (MQ=14)
                    aGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATcc                                                                                                                        >  1:329089/1‑140 (MQ=255)
                      gTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCt                                                                                                                      <  2:254857/140‑1 (MQ=255)
                      gTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCt                                                                                                                      <  2:358208/140‑1 (MQ=255)
                                tcAACGTGCGGGTGTTTTCTCTTTCCAGCAACCAATGCCACCATGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTg                                                                                                            >  1:1619580/1‑140 (MQ=255)
                                tcAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTg                                                                                                            >  1:727739/1‑140 (MQ=39)
                                  aaCGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCa                                                                                                          <  2:1252091/140‑1 (MQ=39)
                                  aaCGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCa                                                                                                          <  1:1639216/140‑1 (MQ=39)
                                       gCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGaa                                                                                                     >  2:1365114/1‑140 (MQ=39)
                                        cGGGGGTTTTGTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGaa                                                                                                     <  2:557741/140‑1 (MQ=0)
                                        cGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGaa                                                                                                     >  2:980713/1‑140 (MQ=0)
                                          ggggTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag                                                                                                  <  2:359981/140‑1 (MQ=39)
                                          ggggTGTTCTCGTGCCCGCACCCATTGCCACCCGGGATAACGCCCCGCAACAGTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag                                                                                                  <  2:1049082/140‑1 (MQ=14)
                                                       tCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACTCCGGCATGGTGGGGATACTACGTCGCAATTCATCTTCAAATCATCCCTGTAATGTTTGCAATGAAGAGTACCTTCCGGCtt                                                                                     >  2:1043336/1‑140 (MQ=14)
                                                       tCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGa                                                                                                      >  2:980919/1‑123 (MQ=40)
                                                       tCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGa                                                                                                      <  1:980919/123‑1 (MQ=40)
                                                             aaCCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAAc                                                                               >  2:304862/1‑140 (MQ=255)
                                                             aaCCAAGGCCACACGGGTTAATGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAAc                                                                               <  1:280238/140‑1 (MQ=14)
                                                              aCCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAAt                                                                                                        <  2:448244/114‑1 (MQ=40)
                                                               ccAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAg                                                                             >  1:847970/1‑140 (MQ=255)
                                                                       accaGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCa                                                                     <  1:707645/140‑1 (MQ=255)
                                                                         caGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGt                                                                   >  1:912510/1‑140 (MQ=255)
                                                                          tgggATAACGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTg                                                                  <  1:1179643/139‑1 (MQ=255)
                                                                          aGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATACCGGCTTTTCAACAGCTGTTGCAGTg                                                                  <  2:1330598/140‑1 (MQ=255)
                                                                           gggATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTgg                                                                 >  2:31863/1‑140 (MQ=255)
                                                                               tAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTc                                                             >  1:242040/1‑140 (MQ=255)
                                                                                aaaGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGt                                                           >  1:983467/1‑140 (MQ=255)
                                                                                  aGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTg                                                          <  1:1342998/140‑1 (MQ=255)
                                                                                  aGCACCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCAGCTTCAATTCATCCCTGACTGGTTTGCAGTGACGAGGTCAGTCCGGCGTTTCCGCAGCTGGTGCGGTGGTTTCggg                                                          >  2:266179/1‑138 (MQ=14)
                                                                                     ccccGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCa                                                        <  2:313062/140‑1 (MQ=255)
                                                                                     ccccGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCa                                                        <  1:518291/140‑1 (MQ=255)
                                                                                     ccccGCAACATTGCGCCTCACCGGATAACGCCGGCATGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCa                                                        <  2:899471/140‑1 (MQ=255)
                                                                                       ccGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTg                                                          <  1:1343077/136‑1 (MQ=21)
                                                                                       ccGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTg                                                          >  2:1343077/1‑136 (MQ=21)
                                                                                       ccGCAACATTGCGCCTCACCGGATAACTCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTg                                                          <  1:1343111/136‑1 (MQ=255)
                                                                                        cGCAACATTGCGCCTCACCGGATAACGCCTGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAggg                                                     >  2:788847/1‑140 (MQ=255)
                                                                                        cGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATAGTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGTGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAggg                                                     >  2:1195097/1‑140 (MQ=255)
                                                                                        cGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCACTGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAggg                                                     >  2:1087284/1‑140 (MQ=255)
                                                                                        cGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAggg                                                     >  2:1238282/1‑140 (MQ=255)
                                                                                        cGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAggg                                                     >  1:301965/1‑140 (MQ=255)
                                                                                          ctaCATTGCGCCTCACCGGATAACTCCGGCTTGGTGTGGATACTAAGTCGCTATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGATGTGTTCATTCCGGGTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGgtg                                                   <  2:1654366/138‑1 (MQ=14)
                                                                                               ttGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTa                                              >  1:1211194/1‑140 (MQ=255)
                                                                                               ttGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTa                                              >  2:1621935/1‑140 (MQ=255)
                                                                                               ttGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACAACTTCGCGACTCTTCTTCACTTCGTCGCTGTGTTGTTTGCAATGAGGTGTACATTACGGGTTTTAATAAGCTGTTGGTGTTGTTTGGGGAAGGGggtggtt                                              >  2:1626125/1‑139 (MQ=9)
                                                                                                    ccTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCGTGCAGGGTGTGGTACAGGc                                         >  2:931968/1‑140 (MQ=255)
                                                                                                     cTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCt                                        >  1:210003/1‑140 (MQ=255)
                                                                                                     cTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCt                                        >  1:209974/1‑140 (MQ=255)
                                                                                                       cACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCg                                      >  1:815118/1‑140 (MQ=255)
                                                                                                            gATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGTAAGGTTTGCCAGGACGAGTACAATCCGGCTGTTCAACAGCGGTTGGAGTGGTGTCGGGCTGGGGGTGGGAGAGGCTCGctctt                                 >  2:1679034/1‑136 (MQ=17)
                                                                                                              tAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAATTTTTTGCAATGATTAGTAATTTCCGGCTTTTCTACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCt                               <  1:886432/140‑1 (MQ=21)
                                                                                                              tAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCt                               <  1:726349/140‑1 (MQ=255)
                                                                                                              tAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCt                               <  1:726503/140‑1 (MQ=255)
                                                                                                               aaCGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTg                              >  1:794924/1‑140 (MQ=255)
                                                                                                               aaCGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTg                              >  1:636930/1‑140 (MQ=255)
                                                                                                                 cGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGAt                            >  1:793047/1‑140 (MQ=255)
                                                                                                                      gCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGGTTTTCAACAGCTGTTGCAGTCTTTTCGTGATGGATGTGGTAGAGGCTACCAACTCTGATTAAct                       >  1:961570/1‑139 (MQ=14)
                                                                                                                      gCTTGGTGTGGATACTACGACGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCAGGATTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCACGCAAATCTGATTAACg                       >  1:961263/1‑140 (MQ=18)
                                                                                                                       cTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGa                      <  2:207415/140‑1 (MQ=255)
                                                                                                                       cTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGa                      <  1:931968/140‑1 (MQ=255)
                                                                                                                        ttGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGAc                     <  2:1615409/140‑1 (MQ=255)
                                                                                                                        ttGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAACTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGAc                     <  1:548258/140‑1 (MQ=255)
                                                                                                                        ttGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGAc                     <  1:1021462/140‑1 (MQ=255)
                                                                                                                        ttGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGAc                     <  1:304862/140‑1 (MQ=255)
                                                                                                                        ttGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGAc                     <  2:1438808/140‑1 (MQ=255)
                                                                                                                        ttGGTGTGGATACTACGTCGCAATTCATCGTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGAGCAGGGTGTGGTACAGGCTCGCAATTCAGATTAACGAc                     <  2:1615479/140‑1 (MQ=255)
                                                                                                                         tGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGa                                                                                                      <  1:1547487/58‑1 (MQ=40)
                                                                                                                         tGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGAcc                    <  1:1678232/140‑1 (MQ=255)
                                                                                                                           gtgtGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAAc                                                                               <  2:1567445/79‑1 (MQ=39)
                                                                                                                             gtgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTc                <  1:980713/140‑1 (MQ=255)
                                                                                                                                gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCa                                                        <  2:587366/97‑1 (MQ=255)
                                                                                                                                gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCa                                                        >  1:587366/1‑97 (MQ=255)
                                                                                                                                 aTACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAgg            <  1:1300909/140‑1 (MQ=255)
                                                                                                                                 aTACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAgg            <  1:1234072/140‑1 (MQ=255)
                                                                                                                                 aTACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAgg            <  1:584773/140‑1 (MQ=255)
                                                                                                                                 aTACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAgg            <  2:1686695/140‑1 (MQ=255)
                                                                                                                                      acGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGgtagta       <  1:879124/140‑1 (MQ=255)
                                                                                                                                          cGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATgaa   >  1:237836/1‑140 (MQ=255)
                                                                                                                                          cGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATgaa   >  2:141543/1‑140 (MQ=255)
                                                                                                                                          cGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATgaa   >  1:845603/1‑140 (MQ=255)
                                                                                                                                           gCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATgaag  <  2:1619580/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAG  >  NC_000913/1270407‑1270684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: