Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,331,561 G→A 5.4% D172N (GAC→AAC)  topA → DNA topoisomerase I, omega subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,331,5610GA 5.4% 257.4 / 3.9 74D172N (GAC→AAC) topADNA topoisomerase I, omega subunit
Reads supporting (aligned to +/- strand):  ref base G (35/35);  new base A (2/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

TGATTGGGGGTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAA  >  NC_000913/1331424‑1331698
                                                                                                                                         |                                                                                                                                         
tGATTGGGGGTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGAc                                                                                                                                         >  2:1104473/1‑140 (MQ=255)
 gATTGGGGGTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGAcc                                                                                                                                        <  1:1614752/140‑1 (MQ=255)
   ttGGGGGTGATGATGCGCGCTATCGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCACCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAAGGCCCAGCAGGCGCGTCGCTTTATGGACcgc                                                                                                                                      <  1:896423/140‑1 (MQ=255)
    tGGGGGTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACcgcg                                                                                                                                     <  2:1171174/140‑1 (MQ=255)
      ggggTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCgtg                                                                                                                                   <  1:522231/140‑1 (MQ=255)
      ggggTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCgtg                                                                                                                                   <  1:1500245/140‑1 (MQ=255)
      ggggTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCgtg                                                                                                                                   <  1:1453485/140‑1 (MQ=255)
        ggTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACACACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCgtggt                                                                                                                                 <  2:944413/140‑1 (MQ=255)
        ggTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCgtggt                                                                                                                                 <  2:1503051/140‑1 (MQ=255)
        ggTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCgtggt                                                                                                                                 <  1:891916/140‑1 (MQ=255)
        ggTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCgtggt                                                                                                                                 <  2:520866/140‑1 (MQ=255)
        ggTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCgtggt                                                                                                                                 <  1:434908/140‑1 (MQ=255)
        ggTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCgtggt                                                                                                                                 <  1:432116/140‑1 (MQ=255)
        ggTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCgtggt                                                                                                                                 <  1:1421687/140‑1 (MQ=255)
        ggTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCgtggt                                                                                                                                 <  1:1373598/140‑1 (MQ=255)
        ggTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAAGGCCTACAAACGCGATCCGCCAGGCATTTAACACACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCgtggg                                                                                                                                 <  2:168428/140‑2 (MQ=255)
         gTGATGATGCGAGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCgtggtg                                                                                                                                <  1:17046/140‑1 (MQ=255)
          tgatgaTGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGAACCGCgtggtg                                                                                                                                >  1:1115678/1‑139 (MQ=255)
          tgatgaTGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGAACCGCgtggtg                                                                                                                                >  1:1115753/1‑139 (MQ=255)
          tgatgaTGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGAACCGCgtggtg                                                                                                                                <  2:1115678/139‑1 (MQ=255)
          tgatgaTGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGAACCGCgtggtg                                                                                                                                <  2:1115753/139‑1 (MQ=255)
             tgatgCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGt                                                                                                                            <  2:1083080/140‑1 (MQ=255)
                 gcgcgcTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTatat                                                                                                                        >  1:392656/1‑140 (MQ=255)
                 gcgcgcTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTatat                                                                                                                        >  1:614519/1‑140 (MQ=255)
                      cTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGttt                                                                                                                   <  2:774554/140‑1 (MQ=255)
                           gCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTcgccg                                                                                                              <  2:696521/140‑1 (MQ=255)
                              gAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCgctg                                                                                                           >  1:1332506/1‑140 (MQ=255)
                               aGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGTCATTTAACAAACCGGGTGAGCTGAAAATTGATCGTTTTAATGCCCAGCAGGCGCGTAGCTATATGGACCGCGTGGTGGGGTATAAGGTTTCGCCgctgc                                                                                                          >  2:1427462/1‑140 (MQ=255)
                               aGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCgctgc                                                                                                          >  2:1112001/1‑140 (MQ=255)
                               aGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCgctgc                                                                                                          >  1:1267359/1‑140 (MQ=255)
                               aGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCgctgc                                                                                                          >  2:1112036/1‑140 (MQ=255)
                                 tggtgTTTAACGAAATTACTAAACACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTa                                                                                                        <  1:1109271/140‑1 (MQ=255)
                                 tggtgTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTa                                                                                                        >  1:321150/1‑140 (MQ=255)
                                 tggtgTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTa                                                                                                        <  1:1109282/140‑1 (MQ=255)
                                 tggtgTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTa                                                                                                        >  1:68443/1‑140 (MQ=255)
                                 tggtgTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTa                                                                                                        >  1:68442/1‑140 (MQ=255)
                                 tggtgTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTa                                                                                                        <  2:582067/140‑1 (MQ=255)
                                   gtgtTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATg                                                                                                      >  1:178700/1‑140 (MQ=255)
                                     gtTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGTATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGa                                                                                                    <  2:1686671/140‑1 (MQ=255)
                                      tttAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATAAGGTTTCGCCGCTGCTATGGaa                                                                                                   >  2:279159/1‑140 (MQ=255)
                                            gAACTTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGACTATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCGTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGAt                                                                                             <  2:572789/140‑1 (MQ=255)
                                            gAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGAt                                                                                             <  1:1032712/140‑1 (MQ=255)
                                            gAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGAt                                                                                             <  2:1356277/140‑1 (MQ=255)
                                                        aaCGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGAACGTGTTAATGCCCAGCAGCCGCGTCGCTATATGGACCGCGTGGTGGGGAATATGGTTACGCCGCTGCTAAGGAAAAAGATCGCTCGAGGCCt                                                                                 >  2:812504/1‑140 (MQ=255)
                                                              aTCCGCCAGTCATTTAACAAACCGGGTGAGCTGATTATTGATCGTGTTAATGCCCATCATGCGCGTCTATTTATGGACCTCGTTGTGGGTTATATTTTTTCGCCGCTGCTATGTAAAAAGATCGCTCGTGGCCTGTATGc                                                                           <  2:1080993/140‑1 (MQ=255)
                                                              aTCCGCCAGGCATTTAACACACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGc                                                                           <  1:297207/140‑1 (MQ=255)
                                                              aTCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATAGTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGGTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGc                                                                           <  1:297249/140‑1 (MQ=255)
                                                                ccGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCg                                                                         <  2:653716/140‑1 (MQ=255)
                                                                        cATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGtgtg                                                                 <  1:1407604/140‑1 (MQ=255)
                                                                                 aaCCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTg                                                        >  2:1225337/1‑140 (MQ=255)
                                                                                 aaCCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTg                                                        >  2:800961/1‑140 (MQ=255)
                                                                                 aaCCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTg                                                        >  2:1542485/1‑140 (MQ=255)
                                                                                 aaCCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGACGGTg                                                        >  2:1225285/1‑140 (MQ=255)
                                                                                  aCCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTgg                                                       >  1:970917/1‑140 (MQ=255)
                                                                                     gggTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCgg                                                    >  1:329052/1‑140 (MQ=255)
                                                                                                      aTCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgag                                   >  2:99353/1‑140 (MQ=255)
                                                                                                        cGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcg                                 >  1:1021941/1‑140 (MQ=255)
                                                                                                        cGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcg                                 >  1:1022628/1‑140 (MQ=255)
                                                                                                         gtgtTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCTTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGTCGGTTCGCCTGGTGGTCgagcgt                                >  2:116026/1‑140 (MQ=255)
                                                                                                         gtgtTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCTGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgt                                >  2:116028/1‑140 (MQ=255)
                                                                                                         gtgtTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgt                                >  2:325853/1‑140 (MQ=255)
                                                                                                         gtgtTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgt                                >  1:1689827/1‑140 (MQ=255)
                                                                                                         gtgtTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgt                                >  2:800969/1‑140 (MQ=255)
                                                                                                         gtgtTAATGACCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgt                                >  2:904063/1‑140 (MQ=255)
                                                                                                           gtTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCgagcgtga                              >  1:737351/1‑140 (MQ=255)
                                                                                                                tGCCCAGCAGGAGCGTCTCTTTATGGACCTCGTGCTTGGGTATATGGTTTCGCCGCTGCTAGGGAAAAAGAAGGCTCGTGGCCTGACTTCCGGTCGTGTGCAGTCGGTGGCGGTTAGCCTGTTGGTAGagcgtgatcgtg                         <  2:432882/140‑1 (MQ=255)
                                                                                                                  cccAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGaa                       >  1:1557896/1‑140 (MQ=255)
                                                                                                                      gcagGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTa                   >  2:1429895/1‑140 (MQ=255)
                                                                                                                      gcagGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTa                   >  1:660901/1‑140 (MQ=255)
                                                                                                                      gcagGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTa                   >  1:197065/1‑140 (MQ=255)
                                                                                                                             cgTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGGGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGtt            <  1:142459/140‑1 (MQ=255)
                                                                                                                                      aTGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGa   >  2:1249916/1‑140 (MQ=255)
                                                                                                                                       tGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGaa  <  1:51525/140‑1 (MQ=255)
                                                                                                                                       tGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGaa  <  1:790650/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TGATTGGGGGTGATGATGCGCGCTATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGCCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTTCGCCTGGTGGTCGAGCGTGAGCGTGAAATTAAAGCGTTCGTGCCGGAA  >  NC_000913/1331424‑1331698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: