Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,513,266 G→T 6.5% V150V (GTG→GTT ydcT → putative ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,513,2660GT 6.5% 244.3 / 2.7 69V150V (GTG→GTTydcTputative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (36/28);  new base T (3/2);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCG  >  NC_000913/1513131‑1513405
                                                                                                                                       |                                                                                                                                           
cGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGtgaatg                                                                                                                                         >  1:890080/1‑140 (MQ=255)
cGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGtgaatg                                                                                                                                         >  1:1188462/1‑140 (MQ=255)
  tGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACCACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGtgaatgaa                                                                                                                                       >  1:1313383/1‑140 (MQ=255)
  tGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCCTTGGtgaatgaa                                                                                                                                       >  1:1313402/1‑140 (MQ=255)
  tGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTGTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGtgaatgaa                                                                                                                                       >  1:1313401/1‑140 (MQ=255)
         aaaGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTTAATGAACcgcgcg                                                                                                                                >  2:600845/1‑140 (MQ=255)
         aaaGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTTAATGAACcgcgcg                                                                                                                                >  2:600765/1‑140 (MQ=255)
         aaaGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTTAATGAACcgcgcg                                                                                                                                >  2:601077/1‑140 (MQ=255)
         aaaGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACcgcgcg                                                                                                                                <  2:638667/140‑1 (MQ=255)
         aaaGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACcgcgcg                                                                                                                                >  1:720021/1‑140 (MQ=255)
         aaaGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACcgcgcg                                                                                                                                >  1:954446/1‑140 (MQ=255)
         aaaGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACcgcgcg                                                                                                                                >  1:719744/1‑140 (MQ=255)
           agcagcGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTa                                                                                                                              <  1:1481604/140‑1 (MQ=255)
           agcagcGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTa                                                                                                                              <  2:382003/140‑1 (MQ=255)
           agcagcGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTa                                                                                                                              <  1:1482160/140‑1 (MQ=255)
           agcagcGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTa                                                                                                                              <  2:902016/140‑1 (MQ=255)
            gcagcGGCACGCTATGGCGCAAGTGGCGCTGGAGATAGTGGCGTTGGGGTTTGTACTTCATCGTAAACCGTCACAACTTTATGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCTTTGGTGTATGAACCGCGCGTAt                                                                                                                             <  1:1587518/140‑1 (MQ=255)
             cagcGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTAtt                                                                                                                            >  2:1186690/1‑140 (MQ=255)
                cGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCt                                                                                                                         <  1:917752/140‑1 (MQ=255)
                   cACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGtt                                                                                                                      >  1:201642/1‑140 (MQ=255)
                   cACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGtt                                                                                                                      >  1:201655/1‑140 (MQ=255)
                   cACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGtt                                                                                                                      >  1:605673/1‑140 (MQ=255)
                          tGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGaa                                                                                                               >  1:820817/1‑140 (MQ=255)
                                                aGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGAtctcg                                                                                         >  2:185377/1‑139 (MQ=255)
                                                  tGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCaaa                                                                                       <  1:1670177/140‑1 (MQ=255)
                                                     cGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTg                                                                                    >  1:1246658/1‑140 (MQ=255)
                                                     cGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTg                                                                                    >  1:905868/1‑140 (MQ=255)
                                                           ggTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAg                                                                              <  1:1057432/140‑1 (MQ=255)
                                                               tGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGa                                                                          <  1:600593/140‑1 (MQ=255)
                                                               tGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTAGCGCCAGAGCTTTGGTGAATGATCCGCGCGTATTGCTGTTGGATGAACCGCGCGGCGCCCTGGATCTCAAGTTGCGTGAGCAgt                                                                          >  2:783326/1‑139 (MQ=255)
                                                                gTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGAt                                                                         <  1:369735/140‑1 (MQ=255)
                                                                gTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGAt                                                                         <  1:58937/140‑1 (MQ=255)
                                                                gTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGAt                                                                         <  1:59443/140‑1 (MQ=255)
                                                                gTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGAt                                                                         <  1:1369361/140‑1 (MQ=255)
                                                                gTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGAt                                                                         <  1:745696/140‑1 (MQ=255)
                                                                 tACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATg                                                                        >  1:815136/1‑140 (MQ=255)
                                                                 tACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATg                                                                        >  1:1127070/1‑140 (MQ=255)
                                                                 tACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATg                                                                        >  1:1330488/1‑140 (MQ=255)
                                                                    aTCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAg                                                                     >  2:1229833/1‑140 (MQ=255)
                                                                    aTCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAg                                                                     >  2:1466739/1‑140 (MQ=255)
                                                                     tCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGc                                                                    >  2:930370/1‑140 (MQ=255)
                                                                      cAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCt                                                                   >  1:866331/1‑140 (MQ=255)
                                                                      cAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCt                                                                   >  2:1641568/1‑140 (MQ=255)
                                                                      cAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCt                                                                   >  2:1369108/1‑140 (MQ=255)
                                                                         cGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGa                                                                >  1:564263/1‑140 (MQ=255)
                                                                               ccGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGaa                                                          <  2:668081/140‑1 (MQ=255)
                                                                               ccGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGaa                                                          <  1:225868/140‑1 (MQ=255)
                                                                                 gTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGaaaa                                                        <  2:506753/140‑1 (MQ=255)
                                                                                 gTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGaaaa                                                        <  2:1613306/140‑1 (MQ=255)
                                                                                 gTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGaaaa                                                        <  2:1446570/140‑1 (MQ=255)
                                                                                  tCACGACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCTTATTGCTGTTGGATGAACCGCTCGGCTCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGaaaaa                                                       >  2:988130/1‑140 (MQ=255)
                                                                                  tCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGaaaaa                                                       >  2:1098782/1‑140 (MQ=255)
                                                                                   cacaACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGaaaaaa                                                      >  2:678972/1‑140 (MQ=255)
                                                                                     caACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACt                                                    >  2:1038973/1‑140 (MQ=255)
                                                                                      aaCTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTg                                                   >  1:1472704/1‑140 (MQ=255)
                                                                                         tttCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCaa                                                <  1:322503/140‑1 (MQ=255)
                                                                                         tttCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCaa                                                <  1:322382/140‑1 (MQ=255)
                                                                                               gtggtCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGtct                                          <  2:508978/140‑1 (MQ=255)
                                                                                                 ggtCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGtctct                                        <  1:1533525/140‑1 (MQ=255)
                                                                                                     aGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGt                                    >  1:153232/1‑140 (MQ=255)
                                                                                                      tcgcCTGCGGGTTGCTATCGCCAGAGCATTGGTTAATGATCCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGTATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAATCTGCTACAGTCTCTCGGTa                                   <  1:434909/139‑1 (MQ=255)
                                                                                                      gcgcCAGCGGGTTGCTATCGCCAGAGCATTGGTTAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTa                                   <  1:434885/140‑1 (MQ=255)
                                                                                                       cgcCAGCGGGTTGCTAACGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTCGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAAAAGTCTCACgcaat                                  >  2:1394656/1‑136 (MQ=255)
                                                                                                             cGGGTTGCTATCGCCAGAGCATTGGTGAAGGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACttt                            >  1:297137/1‑140 (MQ=255)
                                                                                                                gTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTAt                         <  1:1518208/140‑1 (MQ=255)
                                                                                                                        cGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGTTGCAGCTGGAACTGAAAAATCTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTa                 <  2:201655/140‑1 (MQ=255)
                                                                                                                        cGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTa                 <  2:201642/140‑1 (MQ=255)
                                                                                                                        cGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTa                 <  2:605673/140‑1 (MQ=255)
                                                                                                                          ccAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTAcc               >  2:193701/1‑140 (MQ=255)
                                                                                                                              agCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACg           >  1:398796/1‑140 (MQ=255)
                                                                                                                                   tGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAg      >  2:162255/1‑140 (MQ=255)
                                                                                                                                   tGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAg      >  2:1375171/1‑140 (MQ=255)
                                                                                                                                   tGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAg      >  1:1275510/1‑140 (MQ=255)
                                                                                                                                   tGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAg      >  1:1275402/1‑140 (MQ=255)
                                                                                                                                       gaatgaaCCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGCTCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCg  <  2:1508865/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                           
CGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGGATCTCAAATTGCGTGAGCAGATGCAGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCG  >  NC_000913/1513131‑1513405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: