Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,590,848	C→A	6.0%	intergenic (‑312/+6)	yneL ← / ← hipA	pseudogene, AraC family/inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,590,848	0	C	A	6.0%	226.9 / 2.5	67	intergenic (‑312/+6)	yneL/hipA	pseudogene, AraC family/inactivating GltX kinase facilitating persister formation; toxin of HipAB TA pair; autokinase
Reads supporting (aligned to +/- strand):  ref base C (41/22);  new base A (2/2);  total (43/24)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

TAACGACTACCAGAAAAGCACTTCGCATCACTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACGTTATCCAGTGCTGCTGGAATC  >  NC_000913/1590710‑1590983                                                                                                                                           |                                                                                                                                        tAACGACTACCAGAAAAGCACTTCGCATCACTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCa                                                                                                                                        >  2:1020478/1‑140 (MQ=255)        tACCAGAAAAGCACTTCGCATCACTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCa                                                                                                                                 >  2:1082960/1‑140 (MQ=255)          ccAGAAAAGCACTTCGCATCACTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACt                                                                                                                               <  1:1049383/140‑1 (MQ=255)               aaaGCACTTCGCATCACTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTtacta                                                                                                                          <  2:67757/140‑1 (MQ=255)               aaaGCACTTCGCATCACTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTtacta                                                                                                                          <  2:700804/140‑1 (MQ=255)                  gCACTTCGCATCACTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCg                                                                                                                       >  2:908212/1‑140 (MQ=255)                       tCGCATCACTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATtc                                                                                                                  >  2:1312235/1‑140 (MQ=255)                       tCGCATCACTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGAATtc                                                                                                                  >  2:1312267/1‑140 (MQ=255)                        cGCATCACTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATtct                                                                                                                 <  2:386775/140‑1 (MQ=255)                             cACTCAGACATGATTTAACCTAATATACATTAAGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGAATGTCATCCTCATTAACAATGAGCAAACCCCATATCTCACTTACAACCGTATTCTCGGCt                                                                                                            >  2:875253/1‑140 (MQ=255)                             cACTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCACATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCt                                                                                                            >  2:838528/1‑140 (MQ=255)                              aCTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCtt                                                                                                           <  1:1175457/140‑1 (MQ=255)                              aCTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCtt                                                                                                           <  1:635313/140‑1 (MQ=255)                                tCAGACATGATTTAACATAATATACATTAAGCGCACCAAAATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCAAGCACATTAACAATTACCAAACCCCAGATCTCAAATACAACCGAATTCTCGTCTTaa                                                                                                         >  2:848343/1‑140 (MQ=255)                                       tGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAAAAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCa                                                                                                  >  2:484591/1‑140 (MQ=255)                                         aTTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATg                                                                                                >  1:1280735/1‑140 (MQ=255)                                         aTTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCAATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATg                                                                                                >  1:308669/1‑140 (MQ=255)                                         aTTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCAATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATg                                                                                                >  1:308776/1‑140 (MQ=255)                                          tttAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTTTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGc                                                                                               >  1:1348034/1‑140 (MQ=255)                                          tttAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGc                                                                                               <  1:295494/140‑1 (MQ=255)                                          tttAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCACGGCTTAACCGTCCATGc                                                                                               >  1:1349020/1‑140 (MQ=255)                                            tAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCAATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCaa                                                                                             <  2:308776/140‑1 (MQ=255)                                            tAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCAATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCaa                                                                                             <  2:308669/140‑1 (MQ=255)                                              aCATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATTCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATACACGGCTTAACCGTCCATGCAAcc                                                                                           >  2:1614610/1‑140 (MQ=255)                                                    tataCATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAaca                                                                                     >  1:194024/1‑140 (MQ=255)                                                    tataCATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAaca                                                                                     >  1:321498/1‑140 (MQ=255)                                                           tATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCt                                                                              >  2:163623/1‑140 (MQ=255)                                                             tGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATTCTCATTAACAATGACCAAACCCCATATCTAACTTACTACCGAATTCTCGGCTTAACCCTCCAAGCAACCTCAACAAAATGCatt                                                                            >  2:1215383/1‑140 (MQ=255)                                                             tGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATAACTCACTTACTACCGTAATCTCGGCTTAACCGTCCATGCAACCACAAAACATTGCttt                                                                            >  2:1215083/1‑140 (MQ=255)                                                              gcgcACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGTTCATGATTGTCATTCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCACGGCTTAACCGACCATGCAACCTCAACACATTGCTTTc                                                                           >  2:435461/1‑140 (MQ=255)                                                              gcgcACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTc                                                                           >  2:541756/1‑140 (MQ=255)                                                              gcgcACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTc                                                                           >  2:541702/1‑140 (MQ=255)                                                               cgcACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCa                                                                          >  1:1227203/1‑140 (MQ=255)                                                               cgcACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACAACCGTATTCTCGGCTTAACCGTCCATGCAAACACAACACAATGCTTTCa                                                                          >  1:1094838/1‑140 (MQ=255)                                                               cgcACCAATATAAACCAAGGAAGAATCCAGTCGTATGCGGTCAAGATAGTAAAGCTAATTAACAATGAACAAACCACATATCTCACATACTACCGTATTCTCGGCTTAACCGTCCATGCAACCGCAAAACATTGCTTTCa                                                                          >  1:698807/1‑140 (MQ=255)                                                                  aCCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACt                                                                       >  1:207194/1‑140 (MQ=255)                                                                     aaTATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTTTCATGCTCATTAAAAATGACCAAACCCCATAGCTCACTTACTACCTATTTCTAGGATGAAACGTCGGTGCAACCTCATCACATTGCTTTCTACTGcc                                                                    >  2:52542/1‑140 (MQ=255)                                                                       tataAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGt                                                                  >  1:1601595/1‑140 (MQ=255)                                                                            aacAAGGAAGAATCCAGTCGTTGGCGGTCATGAATGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGAATTCTCGGCTTAAACGTCCATGAAACCTAAACACATTTCTTACAACTGCCGTcacca                                                             >  2:1172732/3‑140 (MQ=255)                                                                              cAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACg                                                           >  1:793387/1‑140 (MQ=255)                                                                                aGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGtt                                                         >  2:1072148/1‑140 (MQ=255)                                                                                 ggAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTtc                                                        >  1:490583/1‑140 (MQ=255)                                                                                             tCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCt                                            >  1:1533118/1‑140 (MQ=255)                                                                                               gTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGt                                          <  2:1620499/140‑1 (MQ=255)                                                                                               gTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGt                                          <  2:17177/140‑1 (MQ=255)                                                                                                             ttGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCt                            <  1:1231111/140‑1 (MQ=255)                                                                                                             ttGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCt                            <  2:216581/140‑1 (MQ=255)                                                                                                                tCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCa                         >  1:639397/1‑140 (MQ=255)                                                                                                                  aTGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACg                       <  1:18139/140‑1 (MQ=255)                                                                                                                  aTGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACg                       <  1:1625312/140‑1 (MQ=255)                                                                                                                  aTGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACg                       <  1:1625254/140‑1 (MQ=255)                                                                                                                  aTGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACg                       <  1:632445/140‑1 (MQ=255)                                                                                                                  aTGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACg                       <  2:415806/140‑1 (MQ=255)                                                                                                                  aTGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACg                       <  2:352078/140‑1 (MQ=255)                                                                                                                  aTGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACg                       <  1:786776/140‑1 (MQ=255)                                                                                                                     cTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACGTTa                    >  2:517751/1‑140 (MQ=255)                                                                                                                     cTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACGTTa                    >  2:517770/1‑140 (MQ=255)                                                                                                                        aTTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACGTTATcc                 >  2:142000/1‑140 (MQ=255)                                                                                                                               aTGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACGTTATCCAGtgctg          >  2:1542420/1‑140 (MQ=255)                                                                                                                               aTGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACGTTATCCAGtgctg          >  2:578186/1‑140 (MQ=255)                                                                                                                                tGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGt                                          >  2:1682469/1‑107 (MQ=255)                                                                                                                                tGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGt                                          <  1:1682469/107‑1 (MQ=255)                                                                                                                                tGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACGTTATCCAGtgctgc         <  2:1267200/140‑1 (MQ=255)                                                                                                                                tGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACGTTATCCAGtgctgc         <  2:717741/140‑1 (MQ=255)                                                                                                                                tGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACGTTATCCAGtgctgc         <  2:1267488/140‑1 (MQ=255)                                                                                                                                tGACCAAACCCCAGATCTCACTTACTACCGTATTCTCGGCTTAACCGACCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACGTTATCCAGtgctgc         >  2:413606/1‑140 (MQ=255)                                                                                                                                       aCCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACGTTATCCAGTGCTGCTGGAATc  >  2:1361887/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                        TAACGACTACCAGAAAAGCACTTCGCATCACTCAGACATGATTTAACATAATATACATTATGCGCACCAATATAAACCAAGGAAGAATCCAGTCGTTGGCGGTCATGATTGTCATGCTCATTAACAATGACCAAACCCCATATCTCACTTACTACCGTATTCTCGGCTTAACCGTCCATGCAACCTCAACACATTGCTTTCAACTGCCGTCACCACGTTCTCCGGAAAATCTGTCGGTAATGAAGTCTTCACGTTATCCAGTGCTGCTGGAATC  >  NC_000913/1590710‑1590983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑