Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,748,497 C→A 7.7% P456H (CCT→CAT)  ydhS → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,748,4970CA 7.7% 197.1 / 3.0 59P456H (CCT→CAT) ydhSputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (27/27);  new base A (2/3);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCT  >  NC_000913/1748361‑1748633
                                                                                                                                        |                                                                                                                                        
gCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTgg                                                                                                                                       >  2:776142/1‑140 (MQ=255)
      ggTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTAcg                                                                                                                                 <  2:341999/140‑1 (MQ=255)
             aCTACAACCTGGTAATTAATGAGTCGCGCACCGTCCTGAAATCGGATGACAACTGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACTACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCATGGGATACGCGAGCaa                                                                                                                          <  2:850689/140‑1 (MQ=255)
             aCTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCaa                                                                                                                          <  2:1522315/140‑1 (MQ=255)
             aCTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCaa                                                                                                                          <  2:446757/140‑1 (MQ=255)
                aCAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTa                                                                                                                       >  2:987601/1‑140 (MQ=255)
                aCAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTa                                                                                                                       >  2:987562/1‑140 (MQ=255)
                      tGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACATCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCATGGGCTACGCGAGCAATTACAGaaa                                                                                                                 >  1:473012/1‑140 (MQ=255)
                      tGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGaaa                                                                                                                 >  2:1269398/1‑140 (MQ=255)
                      tGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCATGGGCTACGCGAGCAATTACAGaaa                                                                                                                 >  1:473039/1‑140 (MQ=255)
                         taaTTACTGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACa                                                                                                              <  2:1649594/139‑1 (MQ=255)
                           cttAATGAGTCGCGCACCGTCCTGTAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTTAAGTGAAAGATATCCCTTTCCCTGGTCTTCGCGAGCAATTACTGAAAACAgg                                                                                                            <  2:744812/139‑1 (MQ=255)
                           cgtattGAGGCGCGCATCGTCCTGAACCCGGCAGACTACAGCTACTGGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTTACGTGAATGATTTCCCTTTCCCTGGGCTACGCGAGCTATTTCAGAAAACCgg                                                                                                            <  2:931401/135‑1 (MQ=255)
                           aTTAATGAGTCGCGCACCGTCCTGAAACCGGAAGACAACAGCGACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAgg                                                                                                            <  2:1339936/140‑1 (MQ=255)
                           aTTAATGAGTCGCGCACCGTCCTGAAAACGTAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACTACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAgg                                                                                                            <  2:249355/140‑1 (MQ=255)
                                 gAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGa                                                                                                      <  1:761314/140‑1 (MQ=255)
                                 gAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGATTGACGTTATTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGa                                                                                                      <  1:1059899/140‑1 (MQ=255)
                                              tCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGtt                                                                                         >  2:367204/1‑140 (MQ=255)
                                              tCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGtt                                                                                         >  2:529439/1‑140 (MQ=255)
                                              tCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGtt                                                                                         >  2:1559597/1‑140 (MQ=255)
                                                  gAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCg                                                                                     <  1:1485141/140‑1 (MQ=255)
                                                  gAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCg                                                                                     <  1:1485239/140‑1 (MQ=255)
                                                  gAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCg                                                                                     <  2:433031/140‑1 (MQ=255)
                                                  gAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCg                                                                                     <  1:1269398/140‑1 (MQ=255)
                                                  gAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCg                                                                                     <  1:477875/140‑1 (MQ=255)
                                                  gAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCg                                                                                     <  1:1148462/140‑1 (MQ=255)
                                                         gAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAATGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATta                                                                              <  2:1438501/140‑1 (MQ=255)
                                                         gAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATta                                                                              <  2:1075355/140‑1 (MQ=255)
                                                          aaGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGTACAACGTCCGCTTAAAGTTAAAGATATCCCTTTCCCTGGGCTAAGCGAGCAATTACATAAAAAAGTGGATGAAATACCTGAAGTTGGCGAAGATtat                                                                             >  2:728847/1‑140 (MQ=255)
                                                                 caGCTACTCGTTTGACGTGTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGAGAAAGATATCCCTTTCCCTGTGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTAc                                                                      >  1:932695/1‑140 (MQ=255)
                                                                    cTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGc                                                                   >  1:920506/1‑140 (MQ=255)
                                                                       cTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAAc                                                                >  1:456274/1‑140 (MQ=255)
                                                                        tCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACTCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAAcc                                                               >  1:1460691/1‑140 (MQ=255)
                                                                                    tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCg                                                   >  2:62031/1‑140 (MQ=255)
                                                                                    tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCg                                                   >  2:62029/1‑140 (MQ=255)
                                                                                    tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCg                                                   <  2:346685/140‑1 (MQ=255)
                                                                                            tGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGcgcg                                           <  1:723423/140‑1 (MQ=255)
                                                                                              cccGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCATGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTa                                         <  2:473039/140‑1 (MQ=255)
                                                                                              cccGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCATGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTa                                         <  2:473012/140‑1 (MQ=255)
                                                                                                  cgGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGt                                     >  2:826895/1‑140 (MQ=255)
                                                                                                      cAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCgg                                 <  1:693569/140‑1 (MQ=255)
                                                                                                         cGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGgcgc                              >  1:1425950/1‑140 (MQ=255)
                                                                                                               cTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGcc                        >  1:287431/1‑140 (MQ=255)
                                                                                                                    aGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGt                   <  1:1003381/140‑1 (MQ=255)
                                                                                                                    aGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGt                   >  2:875782/1‑140 (MQ=255)
                                                                                                                    aGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGt                   >  2:1003381/1‑140 (MQ=255)
                                                                                                                        aaaGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGAt               >  1:161168/1‑140 (MQ=255)
                                                                                                                        aaaGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGAt               >  1:1674156/1‑140 (MQ=255)
                                                                                                                         aaGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATg              <  1:367204/140‑1 (MQ=255)
                                                                                                                         aaGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATg              <  1:468469/140‑1 (MQ=255)
                                                                                                                              atCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTTGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGa         >  1:969339/1‑140 (MQ=255)
                                                                                                                              atCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTTGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGa         >  1:969197/1‑140 (MQ=255)
                                                                                                                              atCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGa         >  1:1489036/1‑140 (MQ=255)
                                                                                                                              atCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGa         >  2:1596292/1‑140 (MQ=255)
                                                                                                                              atCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGa         >  2:1186836/1‑140 (MQ=255)
                                                                                                                               tCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGAc        <  2:1249805/140‑1 (MQ=255)
                                                                                                                               tCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGAc        <  2:103214/140‑1 (MQ=255)
                                                                                                                               tCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGAc        <  2:103128/140‑1 (MQ=255)
                                                                                                                                     tCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCt  >  1:382313/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAGCCT  >  NC_000913/1748361‑1748633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑