Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,763,608 C→A 10.4% V134V (GTG→GTT sufB ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,763,6080CA10.4% 326.5 / 15.2 95V134V (GTG→GTTsufBcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base C (49/35);  new base A (4/6);  total (54/41)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGTAGTAG  >  NC_000913/1763473‑1763743
                                                                                                                                       |                                                                                                                                       
cAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACgg                                                                                                                                     >  2:1358554/1‑140 (MQ=255)
        ggTGATCGTGGATCGCCTCACCAAAGGAACCGACAAAAATTCCCTGCTCCGCCAGGTCGTCGCGATAAGGACTGGGAAGCCAATCTGAGACCAAAAAGGCCTCCACCGCCTCCTCTTGGCCATCCCGAACTGGTACggcg                                                                                                                             >  2:1659388/1‑137 (MQ=255)
        ggTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGccc                                                                                                                             >  1:1231073/1‑140 (MQ=255)
        ggTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGccc                                                                                                                             >  1:247581/1‑140 (MQ=255)
        ggTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGAACGGGAACGccc                                                                                                                             >  1:401590/1‑140 (MQ=255)
        ggTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGACAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGccc                                                                                                                             >  1:389990/1‑140 (MQ=255)
          tGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCaa                                                                                                                           <  2:261532/140‑1 (MQ=255)
          tGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCaa                                                                                                                           <  1:983664/140‑1 (MQ=255)
            aTCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACt                                                                                                                         >  1:1678469/1‑140 (MQ=255)
             tCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTg                                                                                                                        >  1:826925/1‑140 (MQ=255)
             tCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTg                                                                                                                        >  1:90780/1‑140 (MQ=255)
             tCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTg                                                                                                                        >  1:65776/1‑140 (MQ=255)
                  gATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCaa                                                                                                                   >  2:781759/1‑140 (MQ=255)
                     cGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAAcg                                                                                                                <  1:1450/140‑1 (MQ=255)
                       ccTCACCTAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAAcgcc                                                                                                              <  2:749741/140‑1 (MQ=255)
                       ccTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAAcgcc                                                                                                              >  1:349759/1‑140 (MQ=255)
                         tCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAAcgccgc                                                                                                            <  1:351739/140‑1 (MQ=255)
                           aCCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCt                                                                                                          >  2:133757/1‑140 (MQ=255)
                           aCCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCt                                                                                                          >  1:1224273/1‑140 (MQ=255)
                           aCCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTATTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCACAAACGCCGCCt                                                                                                          >  2:629305/1‑140 (MQ=255)
                                  gAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACctc                                                                                                   >  1:1671956/1‑140 (MQ=255)
                                  gAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACctc                                                                                                   >  1:1564676/1‑140 (MQ=255)
                                     cAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTTGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACTCCGCCTCCACCTCttt                                                                                                >  1:1017170/1‑140 (MQ=255)
                                     cAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCttt                                                                                                <  2:1208948/140‑1 (MQ=255)
                                     cAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCttt                                                                                                >  2:1230404/1‑140 (MQ=255)
                                     cAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCttt                                                                                                >  1:1017577/1‑140 (MQ=255)
                                     cAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAGAAGTAGTGGCAACCGAATCTGTGTCGAAATTGGCCGCCACCGCCACCTCTTTGCGTTCGCGTCCGGGAACGCCCAACTGCTCAATCGCCGCCACCACctctgt                                                                                                >  2:90102/1‑138 (MQ=255)
                                     cAGAAAATAATTCCCTGCTCCGCCAGTTGTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCATCTGCTCAAACGCCGCCTCCACctctgt                                                                                                >  2:1691655/1‑138 (MQ=255)
                                          aataatTCCCTGCTCCGCCAGTTTTTCGCTATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCCACACCTATTTGCCTTCACGCACGGTAACGCCCAACTGCTCAAACGAAGCCACCACATCTTTACTTa                                                                                           >  1:360109/1‑140 (MQ=255)
                                          aataatTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTa                                                                                           >  1:319865/1‑140 (MQ=255)
                                                  ccTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTTAGTCGAAAATGGCATCCAACGCCACATCTTTGCCATCCAGCACGGGTACGCCCTACTGCTCAAACGCCGCCTCCACGTCTTGACTGAAAAAGGct                                                                                   >  2:1633368/1‑139 (MQ=255)
                                                    tGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGtt                                                                                 >  1:368530/1‑140 (MQ=255)
                                                    tGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGtt                                                                                 >  1:369008/1‑140 (MQ=255)
                                                      cTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTcg                                                                               <  1:117673/140‑1 (MQ=255)
                                                           ccAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCa                                                                          >  2:848503/1‑140 (MQ=255)
                                                           ccAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCa                                                                          <  2:1406144/140‑1 (MQ=255)
                                                           ccAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCa                                                                          <  1:1650926/140‑1 (MQ=255)
                                                           ccAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCa                                                                          <  1:1018699/140‑1 (MQ=255)
                                                           ccAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCa                                                                          <  1:170601/140‑1 (MQ=255)
                                                           ccAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTAGAAAATGGCATCCACCGCCACCTCATTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCa                                                                          <  2:751433/140‑1 (MQ=255)
                                                           ccAGTTATTCGCGCTAAGTAGTGGCAACCGAACCTGAGTCGAAAATGGCATCCACCGCCCCCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCACTTTACTTAAAAAGGCGTTCGCGCCa                                                                          <  2:751518/140‑1 (MQ=255)
                                                                            gtagtGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGcc                                                         <  2:858661/140‑1 (MQ=255)
                                                                            gtagtGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGcc                                                         <  2:1496631/140‑1 (MQ=255)
                                                                                   cAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCa                                                  <  1:121238/140‑1 (MQ=255)
                                                                                     aCCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGa                                                <  1:177621/140‑1 (MQ=255)
                                                                                     aCCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGAACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGa                                                >  2:709559/1‑140 (MQ=255)
                                                                                       cGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAg                                                 >  2:906311/1‑137 (MQ=255)
                                                                                       cGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAg                                                 <  1:906311/137‑1 (MQ=255)
                                                                                       cGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGAcg                                              >  2:1333758/1‑140 (MQ=255)
                                                                                       cGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGAcg                                              >  2:901644/1‑140 (MQ=255)
                                                                                       cGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCACACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGAcg                                              >  2:873958/1‑140 (MQ=255)
                                                                                             tGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGAACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAg                                        <  2:1508686/140‑1 (MQ=255)
                                                                                                 tCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTgtc                                    >  1:805436/1‑140 (MQ=255)
                                                                                                 tCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTgtc                                    >  1:806652/1‑140 (MQ=255)
                                                                                                 tCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTgtc                                    >  1:162597/1‑140 (MQ=255)
                                                                                                 tCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCGTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTgtc                                    >  1:1391102/1‑140 (MQ=255)
                                                                                                 tCGAAAATGGCATCCACCGCCACCTCTTAGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTgtc                                    >  1:1322797/1‑140 (MQ=255)
                                                                                                    aaaaTGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTgtcgtc                                 >  1:357527/1‑140 (MQ=255)
                                                                                                    aaaaTGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTgtcgtc                                 >  1:507048/1‑140 (MQ=255)
                                                                                                     aaaTGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAACCGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTca                                <  2:151144/140‑1 (MQ=255)
                                                                                                         ggCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAAt                            >  1:1537716/1‑140 (MQ=255)
                                                                                                         ggCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAAt                            >  1:1240866/1‑140 (MQ=255)
                                                                                                               cACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGc                      >  2:1034230/1‑140 (MQ=255)
                                                                                                               cACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGc                      >  2:635112/1‑140 (MQ=255)
                                                                                                               cACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGc                      >  2:354913/1‑140 (MQ=255)
                                                                                                                   gCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCATACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACTCGCTATTGTCGTCACAATTACCGCACGa                  <  2:223120/140‑1 (MQ=255)
                                                                                                                   gCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGa                  <  1:1485400/140‑1 (MQ=255)
                                                                                                                   gCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGa                  <  1:203598/140‑1 (MQ=255)
                                                                                                                        ctctTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTg             <  2:1478906/140‑1 (MQ=255)
                                                                                                                        ctctTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTg             <  2:65776/140‑1 (MQ=255)
                                                                                                                        ctctTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTg             <  2:90780/140‑1 (MQ=255)
                                                                                                                        ctctTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTg             <  2:753877/140‑1 (MQ=255)
                                                                                                                        ctctTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTg             <  2:826925/140‑1 (MQ=255)
                                                                                                                        ctctTTGCCTTCCCGAACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTg             <  1:1110747/140‑1 (MQ=255)
                                                                                                                         tctTTGCCTTCCCGCACGGGAACGCCCAACTGCTCACACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGc            <  2:360792/140‑1 (MQ=255)
                                                                                                                          ctTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCt           >  2:978310/1‑140 (MQ=255)
                                                                                                                                 ttCCCGCACGGGAACGCCCAACTGCTCACACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGagtagt    <  2:1668316/140‑1 (MQ=255)
                                                                                                                                 ttCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAATGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGagtagt    <  2:1668313/140‑1 (MQ=255)
                                                                                                                                 ttCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGagtagt    <  1:404144/140‑1 (MQ=255)
                                                                                                                                 ttCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGagtagt    <  1:1358554/140‑1 (MQ=255)
                                                                                                                                 ttCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGagtagt    <  1:142433/140‑1 (MQ=255)
                                                                                                                                 ttCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGagtagt    <  1:142544/140‑1 (MQ=255)
                                                                                                                                 ttCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGagtagt    <  1:765598/140‑1 (MQ=255)
                                                                                                                                 ttCCCGAACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGagtagt    <  2:250521/140‑1 (MQ=255)
                                                                                                                                 ttCCCGAACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGagtagt    <  1:663798/140‑1 (MQ=255)
                                                                                                                                 ttCCCGAACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGagtagt    <  2:905954/140‑1 (MQ=255)
                                                                                                                                 ttCCCGAACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGagtagt    <  1:1284880/140‑1 (MQ=255)
                                                                                                                                  tCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAgtagta   >  1:63463/1‑140 (MQ=255)
                                                                                                                                  tCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAgtagta   >  1:619992/1‑140 (MQ=255)
                                                                                                                                  tCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAgtagta   >  1:58151/1‑140 (MQ=255)
                                                                                                                                  tCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAgtagta   >  1:392791/1‑140 (MQ=255)
                                                                                                                                  tCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAgtagta   >  1:392657/1‑140 (MQ=255)
                                                                                                                                  tCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAgtagta   >  1:391687/1‑140 (MQ=255)
                                                                                                                                  tCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAgtagta   >  1:1569233/1‑140 (MQ=255)
                                                                                                                                   cccGAACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGtagtag  >  1:839758/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTGCCTTCCCGCACGGGAACGCCCAACTGCTCAAACGCCGCCTCCACCTCTTTACTTAAAAAGGCGTTCGCGCCAGTTTGCTGCACCGCGCCAGGTTCAGACGCGCAAGTGTCGTCACAATTACCGCACGATGGTGCTGAGTAGTAG  >  NC_000913/1763473‑1763743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: