| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,873,568 | C→A | 7.5% | intergenic (+37/‑6) | yeaJ → / → yeaK | putative diguanylate cyclase/aminoacyl‑tRNA editing domain protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,873,568 | 0 | C | A | 7.5% | 175.5 / 3.0 | 53 | intergenic (+37/‑6) | yeaJ/yeaK | putative diguanylate cyclase/aminoacyl‑tRNA editing domain protein |
| Reads supporting (aligned to +/- strand): ref base C (28/21); new base A (2/2); total (30/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01 | |||||||||||
CGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCAC > NC_000913/1873429‑1873707 | cGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGc < 1:857483/140‑1 (MQ=255) tGAAAGTAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGGAGAACAAAACCAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGaa < 1:1619713/140‑1 (MQ=255) gaaagaaaACGATACGTTACATGTAGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCCGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGaaa < 2:1353879/140‑1 (MQ=255) gaaagaaaACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGtaa < 2:961860/140‑1 (MQ=255) gaaagaaaACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGaaa < 2:831401/140‑1 (MQ=255) aCGATACGTTACATGATGCGGATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTACATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCATGAGCGTGAAATGACTGAAATGGCTaaa < 2:1319295/140‑1 (MQ=255) gTTACATGAAGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACCAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAAAGACTGAAATGGCTAAAGGAAGCg > 2:790026/1‑140 (MQ=255) ttACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGt < 2:227145/140‑1 (MQ=255) aCATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGa < 1:1317131/140‑1 (MQ=255) cGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAg > 2:1286378/1‑140 (MQ=255) tataAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCg > 2:579503/1‑140 (MQ=255) taAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGt > 1:558373/1‑140 (MQ=255) gCTTCCGACGAGCGTTAATATCTCAATAAGCAGAACAAAAACAGCCGATCAAGATAACCTTCACTGGCTGTTTGCTTGTAATCTAAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCAACAGCGGTTAAt < 2:1322127/140‑1 (MQ=255) ttCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCg > 2:455803/1‑140 (MQ=255) ccGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCt > 2:727214/1‑140 (MQ=255) ccGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCt > 1:998029/1‑140 (MQ=255) ccGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCt > 1:643404/1‑140 (MQ=255) ccGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCt > 1:1652906/1‑140 (MQ=255) ccGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCt > 1:176716/1‑140 (MQ=255) ccGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCt > 1:642093/1‑140 (MQ=255) ccGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCt > 1:621049/1‑140 (MQ=255) ccGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCt > 1:557626/1‑140 (MQ=255) agggTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGATATGACTGAATTGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTttatta < 2:1203031/137‑1 (MQ=255) aGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGTAGCGTGACGCATCAGCGGTTAATCGCTttatta < 2:1478742/140‑1 (MQ=255) aGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTttatta < 1:736782/140‑1 (MQ=255) gtgtataTGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATca < 2:614387/137‑1 (MQ=255) atatGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACaag < 2:668497/140‑1 (MQ=255) tatGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACaaga < 2:754046/140‑1 (MQ=255) tatGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACaaga < 2:754018/140‑1 (MQ=255) tCAATAAGCAGAACAAAAACAGCCGTTCATGATACCGTGCGGGGGGGGGTTGCTGGGCCTCTCTGGAGCGGGACCTGACGGCAATGGCGATAGGCAGCGGGACGCATCCGCGGTTAAACGCGTTCGTTTCCCACGAAggg > 2:1560938/1‑139 (MQ=255) cAATAAGCAGAACAAAAACAGCCGTTAATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGAGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTg > 2:895561/1‑140 (MQ=255) cAATAAGCAGAACAAAAACAGCCGTTAATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGAGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTg > 2:895380/1‑140 (MQ=255) gAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTcc > 2:1070600/1‑140 (MQ=255) aaCAAAAACTGCCGTTCATGATAACCTTCTGTGGGTGTTTGCTTGTAATCTCAGGTGCGTGAAATGTCTGAAATGGCTAATGGAAGCGTGACGCATCAGCGGTTAGAAGCTTTTATCTCACAATAAGGTGCTGACGTCcg > 2:1658806/1‑140 (MQ=255) aaCAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGTAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCcg > 2:1658768/1‑140 (MQ=255) aaCAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCcg > 1:1458262/1‑140 (MQ=255) aaCAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCcg > 1:731313/1‑140 (MQ=255) gCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTAcc > 1:604106/1‑140 (MQ=255) gCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTAcc > 2:1504132/1‑140 (MQ=255) gCCGTTCATGATAACATTCTGTGGGTGGTTGCGTGTAATCTCAGGAGCGTGACATGACTGAAATGGCTGATGGACGCGTGACGCATCAGCGGTTAATCGCTGTATTTTCACAAGAAGTTGCTGACTTCCGCGTCGTTAcc > 2:825148/1‑140 (MQ=255) tCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGa < 2:734526/140‑1 (MQ=255) aTGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAg < 2:1316907/140‑1 (MQ=255) cTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCggtaggt < 1:636536/140‑1 (MQ=255) cTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCggtaggt < 1:1682712/140‑1 (MQ=255) tgtgGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAAt > 1:497685/1‑140 (MQ=255) ggTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAAtgtg > 2:1282836/1‑140 (MQ=255) gttTGCTTGTAATCTCAGGAGAGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGc < 1:895561/140‑1 (MQ=255) gttTGCTTGTAATCTCAGGAGAGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGc < 1:895380/140‑1 (MQ=255) ttGTATTCTCAGGAGCGTGAAATGACTGAAATGGCAAAAGGATGCGTGACGCATCAGCGGTTAATCGCTTTATTCTCACTAGAAGGTGCTCACGTCCGCGTCGTTAACCATGAAGCGGTAGGTAAATGTGCAGCGGTGTc < 2:1251895/140‑1 (MQ=255) gTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAg > 1:878037/1‑140 (MQ=255) gTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAg > 1:877996/1‑140 (MQ=255) cAGGAGCGTGTAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCg > 1:212552/1‑140 (MQ=255) aGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGt > 1:789986/1‑140 (MQ=255) gCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGCAGGTGCTGACGTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCa < 1:707514/140‑1 (MQ=255) cGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCAc < 2:189529/140‑1 (MQ=255) cGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCAc < 2:187139/140‑1 (MQ=255) cGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCAc < 1:1368072/140‑1 (MQ=255) | CGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCAC > NC_000913/1873429‑1873707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |