Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,905,012 C→T 7.5% T71I (ACA→ATA)  yobD → UPF0266 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,905,0120CT 7.5% 272.1 / 9.5 80T71I (ACA→ATA) yobDUPF0266 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (37/37);  new base T (3/3);  total (40/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

ATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTC  >  NC_000913/1904873‑1905150
                                                                                                                                           |                                                                                                                                          
atcatGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGAc                                                                                                                                            >  1:690873/1‑140 (MQ=255)
 tcatGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACa                                                                                                                                           <  1:1440496/140‑1 (MQ=255)
 tcatGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACa                                                                                                                                           <  2:1435379/140‑1 (MQ=255)
 tcatGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACa                                                                                                                                           <  1:195767/140‑1 (MQ=255)
 tcatGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACa                                                                                                                                           <  2:197110/140‑1 (MQ=255)
 tcatGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACa                                                                                                                                           <  2:200606/140‑1 (MQ=255)
 tcatGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACa                                                                                                                                           <  2:439536/140‑1 (MQ=255)
 tcatGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACa                                                                                                                                           <  1:1163379/140‑1 (MQ=255)
 tcatGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACa                                                                                                                                           <  1:1142192/140‑1 (MQ=255)
 tcatGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACa                                                                                                                                           <  2:929011/140‑1 (MQ=255)
 tcatGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCATTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACa                                                                                                                                           <  2:202573/140‑1 (MQ=255)
     gCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGt                                                                                                                                       >  1:963229/1‑140 (MQ=255)
       cccTCCGTAAAGGCCCCACCCTGATGGCAATTCCATAGCTCCGGAGTGCACCAAACCATACCGATATCTACTTCGGATTGATTGACATTAATATCTATAACAACGTCACGAAACAATGTGCGAAAATAAAGACATGGtta                                                                                                                                     >  2:227056/1‑140 (MQ=255)
       cccGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGtta                                                                                                                                     >  1:1577795/1‑140 (MQ=255)
       cccGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGtta                                                                                                                                     >  1:996971/1‑140 (MQ=255)
       cccGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGtta                                                                                                                                     >  2:1497039/1‑140 (MQ=255)
          gccgTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGttatta                                                                                                                                  >  2:669927/1‑140 (MQ=255)
            cgTAACGGCCCCACCCTGCTGGCTATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAg                                                                                                                                <  1:535170/140‑1 (MQ=255)
                aCGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCa                                                                                                                            <  1:415923/140‑1 (MQ=255)
                aCGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCa                                                                                                                            <  2:727232/140‑1 (MQ=255)
                aCGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCa                                                                                                                            <  2:963229/140‑1 (MQ=255)
                      ccACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGct                                                                                                                      >  2:1998/1‑140 (MQ=255)
                            tgctgGCTATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACTTGGTTATTAAGCGCACTGGCTCTGATg                                                                                                                <  2:1555732/140‑1 (MQ=255)
                            tgctgGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATg                                                                                                                >  2:1385709/1‑140 (MQ=255)
                              ctgGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATggg                                                                                                              <  2:878277/140‑1 (MQ=255)
                              ctgGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATggg                                                                                                              >  2:1176295/1‑140 (MQ=255)
                              ctgGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATggg                                                                                                              >  2:364089/1‑140 (MQ=255)
                                ggCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGtt                                                                                                            <  2:1577795/140‑1 (MQ=255)
                                ggCAATTCCTTTGCTCCGGCGTGCTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGtt                                                                                                            <  2:959344/140‑1 (MQ=255)
                                ggCAATGCCCTTGCACCGGCGTGGTCGCCGCGCTAGCGTTAGCGACGTCGGATTGATTGGCAGTCTTATCTATACCAACGGCCCGAATCAAGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCACTGATGGGtt                                                                                                            <  2:1615422/140‑1 (MQ=255)
                                   aaTTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTAGTAAGCGCACTGGCTCTGATGGGttttt                                                                                                         >  2:1384796/1‑140 (MQ=255)
                                    aTTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTACGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTta                                                                                                        >  1:1531238/1‑140 (MQ=255)
                                      tCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCTTGGTGCGTTAATAACGACATGGTTATTAAGCGCTCTGGCTCTGATGGGTTTTtata                                                                                                      >  1:169910/1‑140 (MQ=255)
                                        cTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTtatata                                                                                                    >  1:451958/1‑140 (MQ=255)
                                        cTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTtatata                                                                                                    >  1:452607/1‑140 (MQ=255)
                                          ttGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATAtt                                                                                                  <  1:1115877/140‑1 (MQ=255)
                                           tGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTc                                                                                                 >  1:225232/1‑140 (MQ=255)
                                                cGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGAt                                                                                            >  2:1082288/1‑140 (MQ=255)
                                                cGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGAt                                                                                            >  2:1083449/1‑140 (MQ=255)
                                                  gCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATcc                                                                                          >  2:1460653/1‑140 (MQ=255)
                                                               gATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGat                                                                             <  2:527966/140‑1 (MQ=255)
                                                               gATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGat                                                                             <  2:528259/140‑1 (MQ=255)
                                                               gATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGat                                                                             <  2:528896/140‑1 (MQ=255)
                                                                     gTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCtt                                                                       >  1:1088744/1‑140 (MQ=255)
                                                                      ttATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCttt                                                                      >  1:815747/1‑140 (MQ=255)
                                                                        aTCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTTGATCCGCGTTCCGAAGATCATCTTTaa                                                                    >  2:724247/1‑140 (MQ=255)
                                                                         tCTTCGTCGGATTGATTGTCCTTCTTATCTATAACAACGGCACGAATCCTGGTGCGTTCATAACGACCGGCGACTGCAGCGCACTCGCTCTGAGGGGAGTTTCTATAATCTGGAACCGCGATCCGACGCACATCtaaaaa                                                                   >  2:1689080/1‑135 (MQ=255)
                                                                             cgtcgGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACaaa                                                               <  1:1362475/140‑1 (MQ=255)
                                                                             cgtcgGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACaaa                                                               <  1:1173971/140‑1 (MQ=255)
                                                                                   attgagtgTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGtt                                                         >  2:1563496/1‑140 (MQ=255)
                                                                                     tgattgTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGtttt                                                       >  2:463188/1‑140 (MQ=255)
                                                                                     tgattgTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGtttt                                                       >  2:53548/1‑140 (MQ=255)
                                                                                     tgattgTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGtttt                                                       >  2:53490/1‑140 (MQ=255)
                                                                                     tgattgTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGtttt                                                       >  2:204991/1‑140 (MQ=255)
                                                                                                 ttATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAAt                                           >  2:1590762/1‑140 (MQ=255)
                                                                                                   aTCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCCATGt                                         >  2:38719/1‑140 (MQ=255)
                                                                                                   aTCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGt                                         >  2:38023/1‑140 (MQ=255)
                                                                                                   aTCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGt                                         <  1:984783/140‑1 (MQ=255)
                                                                                                   aTCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGt                                         <  1:463102/140‑1 (MQ=255)
                                                                                                      tataACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTg                                      >  2:11260/1‑140 (MQ=255)
                                                                                                        taACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGa                                    >  1:51560/1‑140 (MQ=255)
                                                                                                        taACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGa                                    <  2:1069952/140‑1 (MQ=255)
                                                                                                        taACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGa                                    <  1:1497039/140‑1 (MQ=255)
                                                                                                           caacGTCACGAATCATGGTGCGTTAATAACGATATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGa                                    >  1:356722/1‑137 (MQ=255)
                                                                                                           caacGTCACGAATCATGGTGCGTTAATAACGATATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGa                                    >  1:356922/1‑137 (MQ=255)
                                                                                                           caacGTCACGAATCATGGTGCGTTAATAACGATATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGa                                    >  1:356928/1‑137 (MQ=255)
                                                                                                           caacGTCACGAATCATGGTGCGTTAATAACGATATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGa                                    <  2:356722/137‑1 (MQ=255)
                                                                                                           caacGTCACGAATCATGGTGCGTTAATAACGATATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGa                                    <  2:356922/137‑1 (MQ=255)
                                                                                                           caacGTCACGAATCATGGTGCGTTAATAACGATATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGa                                    <  2:356928/137‑1 (MQ=255)
                                                                                                              cGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTGTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGATTGAat                              >  2:1072142/1‑140 (MQ=255)
                                                                                                                  aCGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCTATGTCTGGATTGAATATAg                          <  1:977360/140‑1 (MQ=255)
                                                                                                                  aCGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAg                          <  2:344234/140‑1 (MQ=255)
                                                                                                                  aCGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAg                          <  1:105930/140‑1 (MQ=255)
                                                                                                                         aTGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATc                   >  1:1585211/1‑140 (MQ=255)
                                                                                                                          tGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCa                  <  1:457114/140‑1 (MQ=255)
                                                                                                                                 ttAATAACGACAGGGTGCTTAAGCGCACTGGCTCTGTGGGGTTTTTATATCTTCTGGATCCGCGTTCCGAAGATCATCTTTCAACAACAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGAt           >  2:373371/1‑140 (MQ=255)
                                                                                                                                    ataaCGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGaa        <  1:1385709/140‑1 (MQ=255)
                                                                                                                                     taaCGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAAc       <  2:1401201/140‑1 (MQ=255)
                                                                                                                                     taaCGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAAc       <  2:1402801/140‑1 (MQ=255)
                                                                                                                                          aCATTGTTATTAAGCGCACTGGCTCTTATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTc  >  2:628675/1‑140 (MQ=255)
                                                                                                                                          aCATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTc  <  1:1143376/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
ATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATATATTCTGGATCCGCGTTCCGAAGATCATCTTTAAACAAAAAGGTTTTTTCTTCGCCAATGTCTGGATTGAATATAGCCGAATCAAAGCGATGAACTTGTC  >  NC_000913/1904873‑1905150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: