Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,939,460 A→G 9.1% V245A (GTA→GCA)  lpxM ← myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,939,4600AG 9.1% 131.8 / 5.1 44V245A (GTA→GCA) lpxMmyristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (16/24);  new base G (2/2);  total (18/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

GGACCAACAAAAATCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCT  >  NC_000913/1939322‑1939595
                                                                                                                                          |                                                                                                                                       
ggACCAACAAAAATCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTAc                                                                                                                                        >  1:1131754/1‑140 (MQ=255)
ggACCAACAAAAATCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTAc                                                                                                                                        >  1:1242526/1‑140 (MQ=255)
ggACCAACAAAAATCTCGACTACTTCATTCATCCGACGCGCAATCGTATGAACATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCTTCTTTCCATCATAAATCGGAAACAGCGGTAc                                                                                                                                        >  2:764910/1‑140 (MQ=255)
        aaaaaTCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAAcgcgcg                                                                                                                                <  1:138910/140‑1 (MQ=255)
          aaaTCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCa                                                                                                                              <  1:189340/140‑1 (MQ=255)
          aaaTCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCa                                                                                                                              <  1:603814/140‑1 (MQ=255)
            aTCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCAATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACg                                                                                                                            >  2:761162/1‑140 (MQ=255)
                      cttcATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTGCAACGCGCGCACGGCACACTTtc                                                                                                                  >  1:1142299/1‑140 (MQ=255)
                      cttcATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTGCAACGCGCGCACGGCACACTTtc                                                                                                                  >  1:1144126/1‑140 (MQ=255)
                                     gcgcAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATc                                                                                                   <  2:1617709/140‑1 (MQ=255)
                                                tGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGt                                                                                        <  2:867274/140‑1 (MQ=255)
                                                tGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGt                                                                                        >  1:148855/1‑140 (MQ=255)
                                                tGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGt                                                                                        <  1:1510963/140‑1 (MQ=255)
                                                     atcCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCtt                                                                                   >  2:1158000/1‑140 (MQ=255)
                                                     atcCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCtt                                                                                   >  2:1157742/1‑140 (MQ=255)
                                                                    atcatcCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGa                                                                    >  2:900666/1‑140 (MQ=255)
                                                                    atcatcCATCGGTGGGCGCACCTGAATCGTCAGACGAAGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGTCCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGa                                                                    >  2:1080630/1‑140 (MQ=255)
                                                                    atcatcCATCGGTGGGCGCACCTGAATCGACAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGGACAACGCGCGCACGGCACACTTGCTTCAAACGACCAATCGCGGGCTACGTCGCGTAATAGGTGGCAAAGa                                                                    >  2:1688680/1‑140 (MQ=255)
                                                                     tcatcCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGaa                                                                   >  2:1404082/1‑140 (MQ=255)
                                                                      catccatcGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGaaa                                                                  <  2:1473577/140‑1 (MQ=255)
                                                                        tccatcGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATc                                                                >  1:1298689/1‑140 (MQ=255)
                                                                                  ggCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCg                                                      <  1:900666/140‑1 (MQ=255)
                                                                                    cgcACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTGCAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCt                                                    <  2:1142299/140‑1 (MQ=255)
                                                                                    cgcACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTGCAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCt                                                    <  2:1144126/140‑1 (MQ=255)
                                                                                    cgcACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCt                                                    <  2:1214135/140‑1 (MQ=255)
                                                                                    cgcACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCt                                                    <  2:1376388/140‑1 (MQ=255)
                                                                                                   aGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGGACAACGCGCGCACGGCACACTTTCATCTAACGACCAATCGCGGGCAGCGTCGGTTTATTGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGACagg                                     >  2:24141/1‑138 (MQ=255)
                                                                                                         tGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTg                               >  2:649626/1‑140 (MQ=255)
                                                                                                         tGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTg                               >  2:1434561/1‑140 (MQ=255)
                                                                                                         tGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTg                               >  2:649613/1‑140 (MQ=255)
                                                                                                           cGTCTTGCCATCATAAATTGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGAt                             <  2:510585/140‑1 (MQ=255)
                                                                                                           cGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGAt                             <  2:509205/140‑1 (MQ=255)
                                                                                                                tGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGt                        <  2:715850/140‑1 (MQ=255)
                                                                                                                tGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGt                        <  1:1212541/140‑1 (MQ=255)
                                                                                                                       atAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAaataat                 <  2:161552/140‑1 (MQ=255)
                                                                                                                       atAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAaataat                 <  1:11458/140‑1 (MQ=255)
                                                                                                                            tCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATccc            <  1:1448898/140‑1 (MQ=255)
                                                                                                                               gAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCACTCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAg         <  2:1521788/140‑1 (MQ=255)
                                                                                                                               gAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAg         <  1:584642/140‑1 (MQ=255)
                                                                                                                               gAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAg         <  1:1074432/140‑1 (MQ=255)
                                                                                                                                  aCAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTAc      <  1:683777/140‑1 (MQ=255)
                                                                                                                                   cAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTAcc     <  1:1306177/140‑1 (MQ=255)
                                                                                                                                      cGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCt  <  1:271102/140‑1 (MQ=255)
                                                                                                                                      cGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCt  <  1:715265/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
GGACCAACAAAAATCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCT  >  NC_000913/1939322‑1939595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: