Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,939,460 | A→G | 9.1% | V245A (GTA→GCA) | lpxM ← | myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,939,460 | 0 | A | G | 9.1% | 131.8 / 5.1 | 44 | V245A (GTA→GCA) | lpxM | myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase |
Reads supporting (aligned to +/- strand): ref base A (16/24); new base G (2/2); total (18/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 |
GGACCAACAAAAATCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCT > NC_000913/1939322‑1939595 | ggACCAACAAAAATCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTAc > 1:1131754/1‑140 (MQ=255) ggACCAACAAAAATCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTAc > 1:1242526/1‑140 (MQ=255) ggACCAACAAAAATCTCGACTACTTCATTCATCCGACGCGCAATCGTATGAACATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCTTCTTTCCATCATAAATCGGAAACAGCGGTAc > 2:764910/1‑140 (MQ=255) aaaaaTCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAAcgcgcg < 1:138910/140‑1 (MQ=255) aaaTCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCa < 1:189340/140‑1 (MQ=255) aaaTCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCa < 1:603814/140‑1 (MQ=255) aTCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCAATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACg > 2:761162/1‑140 (MQ=255) cttcATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTGCAACGCGCGCACGGCACACTTtc > 1:1142299/1‑140 (MQ=255) cttcATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTGCAACGCGCGCACGGCACACTTtc > 1:1144126/1‑140 (MQ=255) gcgcAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATc < 2:1617709/140‑1 (MQ=255) tGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGt < 2:867274/140‑1 (MQ=255) tGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGt > 1:148855/1‑140 (MQ=255) tGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGt < 1:1510963/140‑1 (MQ=255) atcCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCtt > 2:1158000/1‑140 (MQ=255) atcCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCtt > 2:1157742/1‑140 (MQ=255) atcatcCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGa > 2:900666/1‑140 (MQ=255) atcatcCATCGGTGGGCGCACCTGAATCGTCAGACGAAGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGTCCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGa > 2:1080630/1‑140 (MQ=255) atcatcCATCGGTGGGCGCACCTGAATCGACAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGGACAACGCGCGCACGGCACACTTGCTTCAAACGACCAATCGCGGGCTACGTCGCGTAATAGGTGGCAAAGa > 2:1688680/1‑140 (MQ=255) tcatcCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGaa > 2:1404082/1‑140 (MQ=255) catccatcGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGaaa < 2:1473577/140‑1 (MQ=255) tccatcGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATc > 1:1298689/1‑140 (MQ=255) ggCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCg < 1:900666/140‑1 (MQ=255) cgcACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTGCAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCt < 2:1142299/140‑1 (MQ=255) cgcACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTGCAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCt < 2:1144126/140‑1 (MQ=255) cgcACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCt < 2:1214135/140‑1 (MQ=255) cgcACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCt < 2:1376388/140‑1 (MQ=255) aGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGGACAACGCGCGCACGGCACACTTTCATCTAACGACCAATCGCGGGCAGCGTCGGTTTATTGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGACagg > 2:24141/1‑138 (MQ=255) tGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTg > 2:649626/1‑140 (MQ=255) tGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTg > 2:1434561/1‑140 (MQ=255) tGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTg > 2:649613/1‑140 (MQ=255) cGTCTTGCCATCATAAATTGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGAt < 2:510585/140‑1 (MQ=255) cGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGAt < 2:509205/140‑1 (MQ=255) tGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGt < 2:715850/140‑1 (MQ=255) tGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGt < 1:1212541/140‑1 (MQ=255) atAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAaataat < 2:161552/140‑1 (MQ=255) atAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAaataat < 1:11458/140‑1 (MQ=255) tCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATccc < 1:1448898/140‑1 (MQ=255) gAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCACTCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAg < 2:1521788/140‑1 (MQ=255) gAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAg < 1:584642/140‑1 (MQ=255) gAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAg < 1:1074432/140‑1 (MQ=255) aCAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTAc < 1:683777/140‑1 (MQ=255) cAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTAcc < 1:1306177/140‑1 (MQ=255) cGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCt < 1:271102/140‑1 (MQ=255) cGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCt < 1:715265/140‑1 (MQ=255) | GGACCAACAAAAATCTCGACTTCTTCATTCATCCGCCGCGCAATCGTATGATCATCCGCCTCTAACAGATCATCCATCGGTGGGCGCACCTGAATCGTCAGACGATGCGTCTTGCCATCATAAATCGGAAACAGCGGTACAACGCGCGCACGGCACACTTTCATCAAACGACCAATCGCGGGCAACGTCGCTTTATAGGTGGCAAAGAAATCCACAAATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCT > NC_000913/1939322‑1939595 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |