Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,976,145 C→A 5.0% M37I (ATG→ATT motB ← protein that enables flagellar motor rotation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,976,1450CA 5.0% 288.4 / 2.3 79M37I (ATG→ATTmotBprotein that enables flagellar motor rotation
Reads supporting (aligned to +/- strand):  ref base C (41/34);  new base A (2/2);  total (43/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

TGGGCTTTCACTATTAGAAATGCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCG  >  NC_000913/1976010‑1976283
                                                                                                                                        |                                                                                                                                          
tGGGCTTTCTCTATTACAAATGCGATCGCCGCCCGTAACCGCAGTAGCCAGTGGAGTCCGGAAGTTCTCCTCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCg                                                                                                                                        <  2:848867/140‑1 (MQ=255)
tGGGCTTTCACTATTAGAAATGCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCg                                                                                                                                        <  1:366797/140‑1 (MQ=255)
tGGGCTTTCACTATTAGAAATGCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCg                                                                                                                                        <  1:366910/140‑1 (MQ=255)
tGGGCTTTCACTATTAGAAATGCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCg                                                                                                                                        >  1:1289890/1‑140 (MQ=255)
tGGGCTTTCACTATTAGAAATGCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCg                                                                                                                                        <  1:742390/140‑1 (MQ=255)
tGGGCTTTCACTATTAGAAATGCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCg                                                                                                                                        >  1:732469/1‑140 (MQ=255)
  ggCTTTCACTATTAGAAATGCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCa                                                                                                                                      >  2:1600773/1‑140 (MQ=255)
           tATTAGAAATGCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAg                                                                                                                             <  1:801587/140‑1 (MQ=255)
                     gCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAg                                                                                                                   <  1:749875/140‑1 (MQ=255)
                     gCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAg                                                                                                                   >  1:1602552/1‑140 (MQ=255)
                     gCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAg                                                                                                                   >  1:52043/1‑140 (MQ=255)
                     gCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAg                                                                                                                   >  1:555451/1‑140 (MQ=255)
                     gCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAg                                                                                                                   <  1:1061247/140‑1 (MQ=255)
                     gCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAg                                                                                                                   >  1:1034513/1‑140 (MQ=255)
                       gATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACTTCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCa                                                                                                                 <  2:907467/140‑1 (MQ=255)
                                   tAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGAt                                                                                                     >  2:1134903/1‑140 (MQ=255)
                                   tAACCGCAGTCGCCAGTGGAGACCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGAt                                                                                                     >  2:970952/1‑140 (MQ=255)
                                     aCCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATcc                                                                                                   <  1:975746/140‑1 (MQ=255)
                                         cAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCCTCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCAtgt                                                                                               >  2:1400503/1‑140 (MQ=255)
                                          aGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCAc                                                                                                        <  1:1268524/130‑1 (MQ=255)
                                          aGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCAc                                                                                                        >  2:1268524/1‑130 (MQ=255)
                                            tCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGTTCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGtgct                                                                                            >  2:1628522/1‑140 (MQ=255)
                                            tCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGtgct                                                                                            >  2:1628664/1‑140 (MQ=255)
                                            tCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGtgct                                                                                            <  2:30918/140‑1 (MQ=255)
                                              gCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGtgctgc                                                                                          >  1:855506/1‑140 (MQ=255)
                                                  gTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCg                                                                                      <  1:1476255/140‑1 (MQ=255)
                                                  gTGGAGTCCGGAAGTACTACGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCAATAATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCg                                                                                      >  2:1393128/1‑140 (MQ=255)
                                                  gTGGAGTCCGGAAGTACTACGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCAATAATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCAGGTGCTGCCCCg                                                                                      >  2:1393201/1‑140 (MQ=255)
                                                   tGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGt                                                                                     >  2:476456/1‑140 (MQ=255)
                                                   tGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGt                                                                                     >  2:476851/1‑140 (MQ=255)
                                                                 aCTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTg                                                                       <  2:264233/140‑1 (MQ=255)
                                                                  cTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGc                                                                      >  2:401255/1‑140 (MQ=255)
                                                                  cTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGc                                                                      >  2:936525/1‑140 (MQ=255)
                                                                  cTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGc                                                                      >  1:285361/1‑140 (MQ=255)
                                                                  cTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGc                                                                      >  2:498962/1‑140 (MQ=255)
                                                                  cTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTGTTGGCTTTGc                                                                      >  1:79329/1‑140 (MQ=255)
                                                                  cTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGACCCGTGGCTTTTGGCTTTGc                                                                      >  2:1362047/1‑140 (MQ=255)
                                                                  cTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCAACAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTACACGATCCCTGTGCAGACACGTGGCTTTTGCCTATGc                                                                      >  1:1587329/1‑140 (MQ=255)
                                                                    ccGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGcgt                                                                    <  2:918997/140‑1 (MQ=255)
                                                                          tCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCCTCATCGCAGTCATAAAGTCGGCATAAGCACTCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCGTTGCGTCGTTTg                                                              >  2:1407725/1‑140 (MQ=255)
                                                                          tCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTg                                                              >  2:1041756/1‑140 (MQ=255)
                                                                          tCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTg                                                              >  2:869941/1‑140 (MQ=255)
                                                                          tCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTg                                                              >  2:244682/1‑140 (MQ=255)
                                                                          tCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTg                                                              >  1:304821/1‑140 (MQ=255)
                                                                          tCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTg                                                              >  1:304719/1‑140 (MQ=255)
                                                                           cTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTga                                                             <  2:1506706/140‑1 (MQ=255)
                                                                            tGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCAATAATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTgac                                                            <  1:1393128/140‑1 (MQ=255)
                                                                            tGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCAATAATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTgac                                                            <  1:1393201/140‑1 (MQ=255)
                                                                               aTCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTgacgac                                                         >  2:290157/1‑140 (MQ=255)
                                                                               aTCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTgacgac                                                         >  2:921501/1‑140 (MQ=255)
                                                                                 cAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACaa                                                       >  1:16503/1‑140 (MQ=255)
                                                                                 cAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACaa                                                       >  1:16343/1‑140 (MQ=255)
                                                                                    ctctTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACaataa                                                    >  1:567381/1‑140 (MQ=255)
                                                                                        tttGGGCTGGAGTTGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTTCGTCGTTTGACGACAATAATCgg                                                <  2:420564/140‑1 (MQ=255)
                                                                                          tGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATACGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGAt                                              <  1:350201/140‑1 (MQ=255)
                                                                                          tGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGAt                                              <  2:1256683/140‑1 (MQ=255)
                                                                                          tGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGAt                                              <  1:1243076/140‑1 (MQ=255)
                                                                                          tGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGAt                                              <  1:1243124/140‑1 (MQ=255)
                                                                                          tGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGAt                                              <  1:826196/140‑1 (MQ=255)
                                                                                          tGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGAt                                              <  1:1683108/140‑1 (MQ=255)
                                                                                          agggCTGGAGACGGACATCAGCAACATCACCACAAACAAGGCCATCATCGCTGTCATAAAGACGGCATAAGCAAACTCCCACGAACCAAGTGCTGCCCCGTGGCATTGGGCTTTGCGTCGTTTGACGACAATAATCCGAt                                              <  2:166835/139‑1 (MQ=255)
                                                                                              cTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATgcgc                                          >  2:313918/1‑140 (MQ=255)
                                                                                               tggagatggagaTCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCGCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCt                                         <  2:1367475/140‑1 (MQ=255)
                                                                                               tggagatggagaTCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAAACGGATGCGCt                                         <  2:1594042/140‑1 (MQ=255)
                                                                                                 gagatggagatCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTg                                       >  2:71773/1‑140 (MQ=255)
                                                                                                 gagatggagatCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTg                                       >  2:1477779/1‑140 (MQ=255)
                                                                                                         gatCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCa                               <  1:1134775/140‑1 (MQ=255)
                                                                                                           tCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATg                             >  2:1357174/1‑140 (MQ=255)
                                                                                                            cAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGc                            <  1:593606/140‑1 (MQ=255)
                                                                                                            cAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGc                            >  1:919879/1‑140 (MQ=255)
                                                                                                              gCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTATGGCTTTGCGTCGTTTGACGACAATCATCGTTTGCGCTTGATTATTCATGAtt                          >  2:161184/1‑140 (MQ=255)
                                                                                                                 acaTCACCAGATAAAAGGCCCACATCGCAGTCATAAACACGGCATAAGCAATCTACCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGTTGCGCTTGATTCTTAATGCTTCCt                       <  1:915157/140‑1 (MQ=255)
                                                                                                                 acaTCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCTCGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGTCGACATTAATAGTATGATATTGATTCTTCATGCTTcat                       <  1:1153348/140‑3 (MQ=255)
                                                                                                                 acaTCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCt                       <  1:763851/140‑1 (MQ=255)
                                                                                                                 acaTCAACAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCt                       <  2:1121947/140‑1 (MQ=255)
                                                                                                                    tCACCAGAAAACAGGCCCTCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATACTCGGATGCGCTTGATTCTTCATGCTTCCTCgg                    <  1:101672/140‑1 (MQ=255)
                                                                                                                     cACCAGAAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTCTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCgg                    <  1:1060892/140‑1 (MQ=255)
                                                                                                                         aGAAAAAATGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTgtc               <  2:920844/140‑1 (MQ=255)
                                                                                                                                   gCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCtgttgt      >  1:1364716/1‑140 (MQ=255)
                                                                                                                                      atcatcGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTTGCTTTTGGCTTTGCGTCGTTTGACGACAATTATCGGATGCGCTTTATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGc   <  2:1151124/140‑1 (MQ=255)
                                                                                                                                      atcatcGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGc   <  1:1673007/140‑1 (MQ=255)
                                                                                                                                       tcatcGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCg  >  1:316371/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
TGGGCTTTCACTATTAGAAATGCGATCGCCGCCCGTAACCGCAGTCGCCAGTGGAGTCCGGAAGTACTCCGCAATCTGAATCAGCTCTTTTGGGCTGGAGATGGAGATCAGCCACATCACCAGAAAAAAGGCCATCATCGCAGTCATAAAGTCGGCATAAGCAATCTTCCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCG  >  NC_000913/1976010‑1976283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: