Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,075,388 G→T 5.1% E204* (GAA→TAA)  yeeR → CP4‑44 prophage; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,075,3880GT 5.1% 355.1 / 4.4 97E204* (GAA→TAA) yeeRCP4‑44 prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base G (46/45);  new base T (2/3);  total (49/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

TACGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAGGGAATTT  >  NC_000913/2075253‑2075522
                                                                                                                                       |                                                                                                                                      
tACGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGaaca                                                                                                                                    >  1:1166345/1‑140 (MQ=255)
  cGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGaacaac                                                                                                                                  >  1:560692/1‑140 (MQ=255)
  cGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGaacaac                                                                                                                                  <  2:769500/140‑1 (MQ=255)
  cGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGaacaac                                                                                                                                  <  1:345282/140‑1 (MQ=255)
  cGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGaacaac                                                                                                                                  <  2:769501/140‑1 (MQ=255)
  cGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGaacaac                                                                                                                                  >  1:978679/1‑140 (MQ=255)
  cGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGaacaac                                                                                                                                  >  1:1399742/1‑140 (MQ=255)
  cGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGaacaac                                                                                                                                  <  2:626137/140‑1 (MQ=255)
  cGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGaacaac                                                                                                                                  >  1:465542/1‑140 (MQ=255)
  cGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGaacaac                                                                                                                                  >  1:679019/1‑140 (MQ=255)
   gATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAAcc                                                                                                                                 >  1:1179010/1‑140 (MQ=255)
    atatTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCg                                                                                                                                <  2:1014968/140‑1 (MQ=255)
    atatTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCg                                                                                                                                <  1:1116836/140‑1 (MQ=255)
    atatTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCg                                                                                                                                <  1:1116752/140‑1 (MQ=255)
    atatTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCg                                                                                                                                <  2:1061483/140‑1 (MQ=255)
     tatTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCgg                                                                                                                               >  1:1124782/1‑140 (MQ=255)
        ttCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGc                                                                                                                            >  1:584061/1‑140 (MQ=255)
          cACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTc                                                                                                                          >  1:1083926/1‑140 (MQ=255)
          cACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTc                                                                                                                          >  1:1083921/1‑140 (MQ=255)
          cACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAACAACAACCGGTGCTc                                                                                                                          >  2:600473/1‑140 (MQ=255)
             cGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAATAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAAc                                                                                                                       >  2:1427963/1‑140 (MQ=255)
             cGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCAGTGCCATCAGCCAGACACTGGAAGAACAACCGGTTCTCAAc                                                                                                                       >  2:1427927/1‑140 (MQ=255)
               tCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAg                                                                                                                     >  2:1589177/1‑140 (MQ=255)
               tCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAg                                                                                                                     <  1:530840/140‑1 (MQ=255)
               tCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAg                                                                                                                     <  1:530848/140‑1 (MQ=255)
               tCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAg                                                                                                                     <  1:1491348/140‑1 (MQ=255)
               tCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAACCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAg                                                                                                                     >  1:1601770/1‑140 (MQ=255)
                cACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGt                                                                                                                    <  2:1501902/140‑1 (MQ=255)
                 aCTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCTTTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTa                                                                                                                   <  2:783417/140‑1 (MQ=255)
                 aCTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTa                                                                                                                   <  2:841325/140‑1 (MQ=255)
                 aCTGAGGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCAGTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGtc                                                                                                                   >  2:1641414/1‑139 (MQ=255)
                  cTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTaa                                                                                                                  >  2:310885/1‑140 (MQ=255)
                    gATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAAt                                                                                                                <  2:1210816/140‑1 (MQ=255)
                         ggtggGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTgg                                                                                                           <  2:1280402/140‑1 (MQ=255)
                          gtggGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGc                                                                                                          >  1:302489/1‑140 (MQ=255)
                           tggGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCTGTGCTCAACAGTAAATCGTGGCt                                                                                                         <  1:1200481/140‑1 (MQ=255)
                                  ggTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGt                                                                                                  >  1:47807/1‑140 (MQ=255)
                                     tgttgtACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTg                                                                                               >  2:126529/1‑140 (MQ=255)
                                     tgttgtACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGAGGCTGACCAGTTTg                                                                                               >  2:123352/1‑140 (MQ=255)
                                        tgtACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCaa                                                                                            <  2:95655/140‑1 (MQ=255)
                                          tACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCaaaa                                                                                          <  2:1166345/140‑1 (MQ=255)
                                          tACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCaaaa                                                                                          <  1:310885/140‑1 (MQ=255)
                                          gaCTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGGGGCTGACCAGTTTGCaaaa                                                                                          <  2:1478376/139‑1 (MQ=255)
                                             tggctggcTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGa                                                                                       >  1:1632638/1‑140 (MQ=255)
                                               gctggctgCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGAtt                                                                                     >  1:57146/1‑140 (MQ=255)
                                               gctggctgCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGAtt                                                                                     >  2:122511/1‑140 (MQ=255)
                                                ctggcttCAATAGTGACGCAGCTATGGGCGGCCAGCCAGATCCAGGCGCAGAAAAAACTGACCTGTGCCAACAGCCAGACACTGGAAGAACAACCGGTGCACAACACTAAATCGTGGCTGACCAGATTGCAAAACGATTa                                                                                    <  1:1300036/140‑1 (MQ=255)
                                                 tggctgCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTAc                                                                                   >  1:454368/1‑140 (MQ=255)
                                                  ggctgCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACa                                                                                  <  1:1589177/140‑1 (MQ=255)
                                                          tAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAATAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCt                                                                          <  1:1040592/140‑1 (MQ=255)
                                                          tAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCt                                                                          <  1:957103/140‑1 (MQ=255)
                                                          tAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCt                                                                          <  1:539749/140‑1 (MQ=255)
                                                          tAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCt                                                                          <  1:539826/140‑1 (MQ=255)
                                                            gTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGa                                                                        <  2:584061/140‑1 (MQ=255)
                                                               aCGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTc                                                                     >  2:1451426/1‑140 (MQ=255)
                                                               aCGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTc                                                                     <  2:679019/140‑1 (MQ=255)
                                                               aCGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTc                                                                     <  2:560692/140‑1 (MQ=255)
                                                                cGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCa                                                                    <  2:1625232/140‑1 (MQ=255)
                                                                 gctgctATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAATCGATTACAGCCTTCCTGACTCAc                                                                   >  2:1439573/1‑140 (MQ=255)
                                                                 gctgctATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCAc                                                                   >  1:999623/1‑140 (MQ=255)
                                                                 gctgctATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCAc                                                                   >  1:1071164/1‑140 (MQ=255)
                                                                 gctgctATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCAc                                                                   >  1:1267618/1‑140 (MQ=255)
                                                                        tGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGa                                                            <  2:1221467/140‑1 (MQ=255)
                                                                         gggCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGTCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAg                                                           <  2:845974/140‑1 (MQ=255)
                                                                              gccagccaGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCAt                                                      <  1:954891/140‑1 (MQ=255)
                                                                               ccagccagATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGaacaac                                                                                                                                  >  2:1096490/1‑63 (MQ=255)
                                                                               ccagccagATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGaacaac                                                                                                                                  <  1:1096490/63‑1 (MQ=255)
                                                                               ccagccagATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAATCGATTACAGCCTTCCTGACTCACTGACGGTGCGCATc                                                     >  2:1431260/1‑140 (MQ=255)
                                                                               ccagccagATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATc                                                     >  1:831758/1‑140 (MQ=255)
                                                                               ccagccagATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATc                                                     >  2:63109/1‑140 (MQ=255)
                                                                               ccagccagATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATc                                                     >  1:1666008/1‑140 (MQ=255)
                                                                               ccagccagATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATc                                                     >  1:1666007/1‑140 (MQ=255)
                                                                                     agctCCAGGCGCTGAAATAACGGACCTGGGCCATCAGCCAGACACTGGAAGCACAACCGGTGCTCAACAGTAAATCGTGGCTGACCCGTTTGCAACACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTg                                               <  2:645301/137‑1 (MQ=255)
                                                                                            ggCGCTTAAAAATCTGTCCTGTGCCATCAGCCAGACACTGTAAGAACAACCGGTGCTCAACAGTAATTCGTGGCTGACCAGTTTGCTAAACGATTACAGCATTCCTTACTCACTGACGGTGCGCTTCTGGCTGACGCTCa                                        <  2:1633119/140‑1 (MQ=255)
                                                                                             gcgcTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCAt                                       >  1:367511/1‑140 (MQ=255)
                                                                                                 tGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAATAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTtct                                   >  1:492322/1‑140 (MQ=255)
                                                                                                 tGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAATAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTtct                                   >  1:1580655/1‑140 (MQ=255)
                                                                                                 tGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTtct                                   >  1:302898/1‑140 (MQ=255)
                                                                                                         cTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGAGATTTTTGCAACACGATTCAAGCCTTCCTGACTCTCTGACTTAGCGAATCTGGCTGACGCTCATTTCTCaaatgat                           <  2:256684/140‑6 (MQ=255)
                                                                                                           gACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCCGTTTGCAAAACGATTACAGCCGTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGCtta                         >  2:1274166/1‑139 (MQ=255)
                                                                                                           gACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGAttt                         >  1:754295/1‑140 (MQ=255)
                                                                                                           gACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGAttt                         >  1:870035/1‑140 (MQ=255)
                                                                                                                 tgCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCggg                   <  1:374892/140‑1 (MQ=255)
                                                                                                                 tgCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCggg                   <  1:254229/140‑1 (MQ=255)
                                                                                                                  gCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTACATCGTGGCTGACCAGTTTGCACAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCgggg                  <  2:951928/140‑1 (MQ=255)
                                                                                                                  gCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCgggg                  <  2:910815/140‑1 (MQ=255)
                                                                                                                      tCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGagag              <  1:543072/140‑1 (MQ=255)
                                                                                                                        aGCCAGACACTGGGAGAACAACCGGTGCTCAACTGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCAGCTGGCTGACGCGGATTTGTCATCGGATTGCGGGGGGAGAGCt            >  2:397039/1‑140 (MQ=255)
                                                                                                                        aGCCAGACACTGGAAGAACAACCGGTGCTCAAAAGTAAATCGTGGCTGCCCAGTTTGCAAATCGATTACAGCCTTCCTGACTCACTGTCGGTGCGCAACTGGCTGACGCTCTGTTCTCAACGGATTTGCCGGGGAGAGCt            >  2:397490/1‑140 (MQ=255)
                                                                                                                           cAGACACTGGAATAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAg         <  2:492322/140‑1 (MQ=255)
                                                                                                                           cAGACACTGGAATAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAg         <  2:1580655/140‑1 (MQ=255)
                                                                                                                           cAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAg         <  2:302898/140‑1 (MQ=255)
                                                                                                                           cAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAg         <  2:1576048/140‑1 (MQ=255)
                                                                                                                           cAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAg         >  1:924357/1‑140 (MQ=255)
                                                                                                                              acacTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAGGGa      >  2:683531/1‑140 (MQ=255)
                                                                                                                               cacTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAGGGaa     <  2:1316476/140‑1 (MQ=255)
                                                                                                                                  tGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAGGGAAttt  >  2:466809/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TACGATATTTCACCGTCACTGATGTGGTGGGGCGGGTTGTTGTACTGGCTGGCTGCATTAGTGACGCTGCTATGGGCGGCCAGCCAGATCCAGGCGCTGAAAAAACTGACCTGTGCCATCAGCCAGACACTGGAAGAACAACCGGTGCTCAACAGTAAATCGTGGCTGACCAGTTTGCAAAACGATTACAGCCTTCCTGACTCACTGACGGAGCGCATCTGGCTGACGCTCATTTCTCAACGGATTTCCCGGGGAGAGCTGAGGGAATTT  >  NC_000913/2075253‑2075522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: