Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,205,701	G→T	7.7%	E3* (GAG→TAG)	yehM →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,205,701	0	G	T	7.7%	268.8 / 7.3	78	E3* (GAG→TAG)	yehM	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (48/24);  new base T (3/3);  total (51/27)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCGGGTAGACGAACTGTTTTT  >  NC_000913/2205564‑2205840                                                                                                                                          |                                                                                                                                            cATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTAGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATgagcgag                                                                                                                                           <  1:1455682/140‑1 (MQ=255) cATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTAGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATgagcgag                                                                                                                                           <  1:1454462/140‑1 (MQ=255)                     aagaTCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcggcatccg                                                                                                                       <  1:192180/140‑1 (MQ=255)                     aagaTCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcggcatccg                                                                                                                       <  2:269429/140‑1 (MQ=255)                     aagaTCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcggcatccg                                                                                                                       <  2:855524/140‑1 (MQ=255)                      agaTCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcggcatccgg                                                                                                                      <  1:1423706/140‑1 (MQ=255)                      agaTCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcggcatccgg                                                                                                                      <  1:659593/140‑1 (MQ=255)                          cgcgcgcgTGTGCGCCGTTAGTTCGAACAGCGTGTCGCGACTCATAAAGTAGCTCACTGGCTGGCTTATTTTCTAGCCCGCCACGGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTcggcatccggcatc                                                                                                                  <  2:1661310/140‑1 (MQ=255)                            cgcgcgTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGcattat                                                                                                                >  1:858609/1‑140 (MQ=255)                                      cgcCGTTACTTCGTACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCAGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCt                                                                                                      >  1:1136302/1‑140 (MQ=255)                                      cgcCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCt                                                                                                      >  1:1101519/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGCGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  2:790673/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCTAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  2:514922/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCTAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  2:514933/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCTAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  2:514942/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  1:99769/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  2:265854/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  2:13368/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  1:1081800/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  1:177930/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  2:950831/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  1:1187631/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  1:1335627/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  1:111798/1‑140 (MQ=255)                                           ttACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGGTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGcc                                                                                                 >  1:93739/1‑140 (MQ=255)                                             aCTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTCATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCg                                                                                               >  2:508881/1‑140 (MQ=255)                                               ttCGAACAGCGTGTCGCCACACATACAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGc                                                                                             <  1:1675060/140‑1 (MQ=255)                                                tCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCCTGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGcg                                                                                            >  2:811913/1‑139 (MQ=255)                                                tCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCt                                                                                            >  2:1266141/1‑140 (MQ=255)                                                tCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCt                                                                                            >  2:1266216/1‑140 (MQ=255)                                                   aaCAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCAGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGt                                                                                         >  1:1114737/1‑140 (MQ=255)                                                        cGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAAt                                                                                    >  1:1628183/1‑140 (MQ=255)                                                        cGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAAt                                                                                    >  1:1629968/1‑140 (MQ=255)                                                        cGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAAt                                                                                    >  2:285275/1‑140 (MQ=255)                                                           gtCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCAACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGt                                                                                 <  1:280917/140‑1 (MQ=255)                                                            tCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGtt                                                                                <  2:456252/140‑1 (MQ=255)                                                            gcGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGtt                                                                                <  2:752275/139‑1 (MQ=255)                                                                  cacaTAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGGAATCGTTAATCGa                                                                          >  2:1665055/1‑140 (MQ=255)                                                                  cacaTAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGa                                                                          >  2:794000/1‑140 (MQ=255)                                                                  cacaTAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGa                                                                          >  1:136190/1‑140 (MQ=255)                                                                  cacaTAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGa                                                                          >  2:1663918/1‑140 (MQ=255)                                                                           aagCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCg                                                                 <  2:1253471/140‑1 (MQ=255)                                                                             gCTCCCTGGCAGGCGAACAATCAACCCCGCCACCGCCTGCCGTGAGGAAAGATGCCTGCGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGc                                                               <  2:495670/140‑1 (MQ=255)                                                                             gCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGc                                                               >  1:888620/1‑140 (MQ=255)                                                                             gCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGc                                                               >  1:419552/1‑140 (MQ=255)                                                                             gCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGc                                                               <  1:246811/140‑1 (MQ=255)                                                                             gCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGc                                                               >  1:1083604/1‑140 (MQ=255)                                                                                 aCTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACg                                                           >  2:1648244/1‑140 (MQ=255)                                                                                  cTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGa                                                          <  1:1483187/140‑1 (MQ=255)                                                                                   tGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGAt                                                         >  2:58283/1‑140 (MQ=255)                                                                                   tGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGAt                                                         >  2:96209/1‑140 (MQ=255)                                                                                   tGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGAt                                                         >  1:1559451/1‑140 (MQ=255)                                                                                     gCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATTAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATAc                                                       >  2:911877/1‑140 (MQ=255)                                                                                     gCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATAc                                                       >  2:1184089/1‑140 (MQ=255)                                                                                     gCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATAc                                                       >  2:1184574/1‑140 (MQ=255)                                                                                        tgCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACgtg                                                    <  1:121464/139‑1 (MQ=255)                                                                                        ggCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACgtg                                                    <  1:121262/140‑1 (MQ=255)                                                                                        ggCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACgtg                                                    <  2:663267/140‑1 (MQ=255)                                                                                         gCTTATTATCACGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGTGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCAGGATACgtgt                                                   <  2:760602/140‑1 (MQ=255)                                                                                         gCTTATTATCAAGCCCGCCTCCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACgtgt                                                   <  2:1369424/140‑1 (MQ=255)                                                                                               tatCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGTTAGGTGttgattg                                             >  2:305239/1‑140 (MQ=255)                                                                                                   aaGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCTAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAgg                                         <  1:514942/140‑1 (MQ=255)                                                                                                   aaGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCTAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAgg                                         <  1:514933/140‑1 (MQ=255)                                                                                                   aaGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCTAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAgg                                         <  1:514922/140‑1 (MQ=255)                                                                                                   aaGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACTAGTTGATTGAAgg                                         >  1:1653707/1‑140 (MQ=255)                                                                                                   aaGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAgg                                         >  1:1653845/1‑140 (MQ=255)                                                                                                     gCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGcc                                       >  2:240676/1‑140 (MQ=255)                                                                                                     gCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGcc                                       >  2:240684/1‑140 (MQ=255)                                                                                                        cGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCgg                                    >  2:104770/1‑140 (MQ=255)                                                                                                          ccACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCt                                  >  2:232691/1‑140 (MQ=255)                                                                                                          ccACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCt                                  >  2:1552956/1‑140 (MQ=255)                                                                                                                ccTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGAtttt                            >  2:155459/1‑140 (MQ=255)                                                                                                                ccTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGAtttt                            >  2:155434/1‑140 (MQ=255)                                                                                                                     cGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGa                       <  1:529819/140‑1 (MQ=255)                                                                                                                     cGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGa                       <  1:1332585/140‑1 (MQ=255)                                                                                                                     cGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGa                       <  1:1332385/140‑1 (MQ=255)                                                                                                                        gAGGAAAGATGCATGAGCGATCCGTTTATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCg                    <  2:1528049/140‑1 (MQ=255)                                                                                                                              aGAAGCGTGAGCGAGCCGTTAATTGTCGGCAACCGGCTTCATTGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGTTACGTGAAGATTGAAGGCCCGGCTCATTTTAATGACCGGGTAGa              >  2:1676922/1‑140 (MQ=255)                                                                                                                                  gCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCGGGTAGACGaa          <  1:385638/140‑1 (MQ=255)                                                                                                                                     tGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCGGGTAGACGAACTg       >  1:352721/1‑140 (MQ=255)                                                                                                                                         cgagcCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCAGGCTGGTGAAATCGTTAATCGAAATCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCGGGTAGACGAACTGtttt   >  1:618469/1‑140 (MQ=255)                                                                                                                                         cgagcCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCAGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCGGGTAGACGAACTGtttt   >  1:1052927/1‑140 (MQ=255)                                                                                                                                          gagcCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCGGGTAGACGAACTGttttt  <  2:608870/140‑1 (MQ=255)                                                                                                                                          gagcCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCGGGTAGACGAACTGttttt  <  2:609584/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                            CATCGTCAAAGATAACGAAGAAGATCGCGCGCGTCTGCGCCGTTACTTCGAACAGCGTGTCGCCACACATAAAGAAGCTCACTGGCAGGCTTATTATCAAGCCCGCCACCGCCTGCCGTGAGGAAAGATGCATGAGCGAGCCGTTAATTGTCGGCATCCGGCATCATAGTCCGGCCTGCGCCCGGCTGGTGAAATCGTTAATCGAAAGCCAGCGGCCACGATACGTGTTGATTGAAGGCCCGGCTGATTTTAATGACCGGGTAGACGAACTGTTTTT  >  NC_000913/2205564‑2205840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑