Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,525,571 C→A 6.7% P149T (CCG→ACG)  yfeN → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,5710CA 6.7% 309.0 / 5.9 89P149T (CCG→ACG) yfeNputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (27/56);  new base A (3/3);  total (30/59)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TGGGTGACACCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAG  >  NC_000913/2525437‑2525705
                                                                                                                                      |                                                                                                                                      
tGGGTGACACCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTc                                                                                                                                   <  2:1637343/140‑1 (MQ=255)
  ggTGACACCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCtt                                                                                                                                 >  1:690483/1‑140 (MQ=255)
  ggTGACACCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCtt                                                                                                                                 >  2:869081/1‑140 (MQ=255)
  ggTGACACCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCtt                                                                                                                                 >  1:979300/1‑140 (MQ=255)
  ggTGACACCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAAACGTTCtt                                                                                                                                 >  2:942449/1‑140 (MQ=255)
  ggTGACACCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAAACGTTCtt                                                                                                                                 >  2:942441/1‑140 (MQ=255)
  ggTGACACCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAAACGTTCtt                                                                                                                                 >  2:942241/1‑140 (MQ=255)
      acacCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCt                                                                                                                             <  1:1530959/140‑1 (MQ=255)
      acacCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCt                                                                                                                             <  2:1624843/140‑1 (MQ=255)
      acacCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCt                                                                                                                             <  2:1624645/140‑1 (MQ=255)
      acacCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCt                                                                                                                             <  1:918905/140‑1 (MQ=255)
      acacCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCt                                                                                                                             <  1:1009379/140‑1 (MQ=255)
      acacCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCt                                                                                                                             <  1:253044/140‑1 (MQ=255)
      acacCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCt                                                                                                                             <  1:1347671/140‑1 (MQ=255)
      acacCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCt                                                                                                                             <  1:1347591/140‑1 (MQ=255)
      acacCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCt                                                                                                                             <  1:1320983/140‑1 (MQ=255)
      acacCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCt                                                                                                                             <  1:1228913/140‑1 (MQ=255)
      acacCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCt                                                                                                                             <  1:1230798/140‑1 (MQ=255)
       cacCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAATCCGTTCTTTGCTa                                                                                                                            >  2:77012/1‑140 (MQ=255)
        acCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTaa                                                                                                                           <  2:1164608/140‑1 (MQ=255)
        acCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTaa                                                                                                                           <  2:101266/140‑1 (MQ=255)
         ccGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAg                                                                                                                          <  1:439054/140‑1 (MQ=255)
         ccGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAg                                                                                                                          <  1:440644/140‑1 (MQ=255)
           ggATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCg                                                                                                                        <  1:1216819/140‑1 (MQ=255)
           ggATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCg                                                                                                                        <  1:1216967/140‑1 (MQ=255)
            gATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGt                                                                                                                       >  2:1305603/1‑140 (MQ=255)
                tAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTtata                                                                                                                   <  1:1424159/140‑1 (MQ=255)
                  aTCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACa                                                                                                                 >  2:1142818/1‑140 (MQ=255)
                        tcTTGCATGCGTATGGCACCTACGGTTATGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCATTGCTAAGCGTTATACAGATCaa                                                                                                           <  1:700415/139‑1 (MQ=255)
                        aCTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCaa                                                                                                           <  2:1017295/140‑1 (MQ=255)
                        aCTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAAACGTTCTTTGCTAAGCGTTATACAGATCaa                                                                                                           <  1:942241/140‑1 (MQ=255)
                        aCTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAAACGTTCTTTGCTAAGCGTTATACAGATCaa                                                                                                           <  1:942441/140‑1 (MQ=255)
                        aCTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAAACGTTCTTTGCTAAGCGTTATACAGATCaa                                                                                                           <  1:942449/140‑1 (MQ=255)
                         cTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCaaa                                                                                                          >  1:1385625/1‑140 (MQ=255)
                           tGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAAcc                                                                                                        <  2:1179601/140‑1 (MQ=255)
                           tGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAAcc                                                                                                        <  2:1127749/140‑1 (MQ=255)
                                 cGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTac                                                                                                  >  2:634987/1‑140 (MQ=255)
                                  gTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTaca                                                                                                 <  1:1079730/140‑1 (MQ=255)
                                             aCGGTTCTGCGAATCGCTTGAATTTCCACGACGATATGTTCCTGAACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAATCGTTATACAGCTCAAACCTATAAAACAGGCGACAAc                                                                                      >  2:1238110/1‑140 (MQ=255)
                                             aCGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACAGGCGACAAc                                                                                      >  2:341680/1‑140 (MQ=255)
                                                   cTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTAt                                                                                >  2:1389963/1‑140 (MQ=255)
                                                   cTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTAt                                                                                >  2:1389901/1‑140 (MQ=255)
                                                    tGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATg                                                                               >  1:941576/1‑140 (MQ=255)
                                                      cGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTc                                                                             <  2:574802/140‑1 (MQ=255)
                                                      cGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTACACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTc                                                                             <  2:40729/140‑1 (MQ=255)
                                                        aaTCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGc                                                                           >  1:457857/1‑140 (MQ=255)
                                                           cgcgTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCgg                                                                        <  1:635099/140‑1 (MQ=255)
                                                                gAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTggg                                                                   >  1:1313439/1‑140 (MQ=255)
                                                                   tttCCACGTCGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTg                                                                <  2:707848/140‑1 (MQ=255)
                                                                   tttCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTg                                                                <  2:767133/140‑1 (MQ=255)
                                                                   tttCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTg                                                                <  1:1389963/140‑1 (MQ=255)
                                                                   tttCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTg                                                                <  1:1389901/140‑1 (MQ=255)
                                                                   ttgccACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGATCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTg                                                                <  1:536687/137‑1 (MQ=255)
                                                                          gacgaTATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTa                                                         <  2:526710/140‑1 (MQ=255)
                                                                          gacgaTATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTa                                                         <  2:289642/140‑1 (MQ=255)
                                                                          gacgaTATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTa                                                         <  1:94842/140‑1 (MQ=255)
                                                                          gacgaTATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTa                                                         <  1:121126/140‑1 (MQ=255)
                                                                          gacgaTATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTa                                                         <  1:799826/140‑1 (MQ=255)
                                                                          gacgaTATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTa                                                         <  1:72898/140‑1 (MQ=255)
                                                                          gacgaTATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTa                                                         <  1:477311/140‑1 (MQ=255)
                                                                          gacgaTATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTa                                                         <  1:670869/140‑1 (MQ=255)
                                                                          gacgaTATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTa                                                         <  1:670833/140‑1 (MQ=255)
                                                                          gacgaTATGTTCCACAACGCTATCGGCAACAATTTCACCGCCACCGGTTGGAGGTTCAAACCGTACTTTGCAAACCGTTATACAGATCAAACCAATAACACTCCCCACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTa                                                         <  2:1541910/140‑1 (MQ=255)
                                                                               tatGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACt                                                    <  1:1272765/140‑1 (MQ=255)
                                                                               tatGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACt                                                    <  2:724108/140‑1 (MQ=255)
                                                                                         tACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTggg                                          >  1:1021436/1‑140 (MQ=255)
                                                                                         tACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTggg                                          <  2:765163/140‑1 (MQ=255)
                                                                                         tACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTggg                                          <  2:331552/140‑1 (MQ=255)
                                                                                            ggTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAg                                       >  2:1125294/1‑140 (MQ=255)
                                                                                            ggTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAg                                       >  2:1125178/1‑140 (MQ=255)
                                                                                              tATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCg                                     <  1:754571/140‑1 (MQ=255)
                                                                                                tCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGGCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCgag                                   >  2:130084/1‑140 (MQ=255)
                                                                                                tCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCgag                                   >  2:179550/1‑140 (MQ=255)
                                                                                                   gCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGaaa                                >  1:1307462/1‑140 (MQ=255)
                                                                                                       cAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACTACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCa                            <  2:1687535/140‑1 (MQ=255)
                                                                                                        aaTTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCAc                           >  1:1625588/1‑140 (MQ=255)
                                                                                                        aaTTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCGATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTctc                           >  1:1625479/1‑138 (MQ=255)
                                                                                                          tttCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCActc                         <  2:1307671/140‑1 (MQ=255)
                                                                                                          tttCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCActc                         >  1:595389/1‑140 (MQ=255)
                                                                                                              aCCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTAc                     >  2:423203/1‑140 (MQ=255)
                                                                                                              aCCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTAc                     >  2:401959/1‑140 (MQ=255)
                                                                                                                      cGGTTGGTGGTTCACACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGa             <  2:1627505/140‑1 (MQ=255)
                                                                                                                      cGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACTACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGa             <  2:1044819/140‑1 (MQ=255)
                                                                                                                      cGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGa             <  1:1654277/140‑1 (MQ=255)
                                                                                                                      cGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGa             >  2:348756/1‑140 (MQ=255)
                                                                                                                       ggTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGaa            <  2:910915/140‑1 (MQ=255)
                                                                                                                             tggtTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAacgagta      <  1:584651/140‑1 (MQ=255)
                                                                                                                              ggtTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAacgagtac     <  1:469274/140‑1 (MQ=255)
                                                                                                                                 tCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAacgagtacgag  <  1:990710/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
TGGGTGACACCGGATTTAATCTCTACTTGCATGCGTATGGCACCTACGGTTCTGCGAATCGCGTGAATTTCCACGACGATATGTTCCTGTACGGTATCGGCTACAATTTCACCGGCAGCGGTTGGTGGTTCAAACCGTTCTTTGCTAAGCGTTATACAGATCAAACCTATTACACTGGCGACAACGGCTATGTCGCCGGCTGGGTTGCAGGTTACAACTTTATGCTGGGCAGCGAGAAATTCACTCTTACCAACTGGAACGAGTACGAG  >  NC_000913/2525437‑2525705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: