Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,076,880 C→A 5.2% D193Y (GAT→TAT)  yggP ← putative Zn‑binding dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,076,8800CA 5.2% 426.1 / 6.3 116D193Y (GAT→TAT) yggPputative Zn‑binding dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (54/56);  new base A (3/3);  total (57/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 116 total aligned reads displayed.

TTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCAC  >  NC_000913/3076744‑3077013
                                                                                                                                        |                                                                                                                                     
ttCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGTCGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGAt                                                                                                                                    >  1:1640084/1‑140 (MQ=255)
ttCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGAt                                                                                                                                    >  2:341601/1‑140 (MQ=255)
ttCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGAt                                                                                                                                    >  1:1639985/1‑140 (MQ=255)
ttCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGAt                                                                                                                                    >  1:1551024/1‑140 (MQ=255)
ttCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGAt                                                                                                                                    >  2:341622/1‑140 (MQ=255)
   acaTACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAgc                                                                                                                                 >  2:739577/1‑140 (MQ=255)
   acaTACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAgc                                                                                                                                 >  2:739064/1‑140 (MQ=255)
   acaTACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAgc                                                                                                                                 >  2:47315/1‑140 (MQ=255)
        cacCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCaa                                                                                                                            >  1:487822/1‑140 (MQ=255)
             gCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGccc                                                                                                                       >  2:1525138/1‑140 (MQ=255)
             gCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGccc                                                                                                                       >  2:348790/1‑140 (MQ=255)
              cTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCa                                                                                                                      >  2:1476945/1‑140 (MQ=255)
              cTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGAAGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAAAGCCCa                                                                                                                      >  2:1476702/1‑140 (MQ=255)
               tCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCAt                                                                                                                     <  1:1600700/140‑1 (MQ=255)
                   tGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCTATAGCTCCAATGCCCATCGGa                                                                                                                 >  2:1289483/1‑140 (MQ=255)
                   tGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGa                                                                                                                 >  2:1060697/1‑140 (MQ=255)
                   tGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAAAGCCCATCGGa                                                                                                                 >  2:1289513/1‑140 (MQ=255)
                   tGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATAGATAGCGCCAATGCCCATCGGa                                                                                                                 >  2:1421030/1‑140 (MQ=255)
                   tGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATAGATAGCGCCAATGCCCATCGGa                                                                                                                 >  2:469675/1‑140 (MQ=255)
                   tGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATAGATAGCGCCAATGCCCATCGGa                                                                                                                 >  2:1420727/1‑140 (MQ=255)
                       tttACTGGCCGCCAGTTCCCCCGGCAGCAGCTTCTGTACCTGCGCCAGTCGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTg                                                                                                             <  2:1406887/140‑1 (MQ=255)
                        ttACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGc                                                                                                            >  1:630633/1‑140 (MQ=255)
                        ttACTGGCCGCCAGTTCCACCGGCAGCAGCTACTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCAACACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAAAGCCCATCGGACCTGc                                                                                                            >  2:792619/1‑140 (MQ=255)
                         tACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGca                                                                                                           <  1:1307255/140‑1 (MQ=255)
                         tACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGca                                                                                                           <  2:118132/140‑1 (MQ=255)
                         tACTGGCCGCCAGGTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCGATCGGACCTGca                                                                                                           <  1:1035260/140‑1 (MQ=255)
                            tGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCacac                                                                                                        <  1:1029866/140‑1 (MQ=255)
                              gccgccAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGc                                                                                                      >  2:538383/1‑140 (MQ=255)
                                      ttCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGATTGTCGTCGATATCGACCACCCCCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCGGTg                                                                                              <  2:903400/140‑1 (MQ=255)
                                             ggCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTTTGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATAtt                                                                                       >  2:1660690/1‑140 (MQ=255)
                                             ggCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATAtt                                                                                       <  1:110311/140‑1 (MQ=255)
                                             ggCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATAtt                                                                                       >  2:1416186/1‑140 (MQ=255)
                                                   agcTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTgccgcc                                                                                 <  1:1288928/140‑1 (MQ=255)
                                                          gTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTg                                                                          >  1:1593273/1‑140 (MQ=255)
                                                              cTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGc                                                                      <  1:365036/140‑1 (MQ=255)
                                                               tGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGcc                                                                     >  2:538697/1‑140 (MQ=255)
                                                               tGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGcc                                                                     >  2:841971/1‑140 (MQ=255)
                                                               tGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGcc                                                                     >  2:226629/1‑140 (MQ=255)
                                                               tGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGcc                                                                     >  2:1262580/1‑140 (MQ=255)
                                                               tGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGAACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGcc                                                                     >  2:475946/1‑140 (MQ=255)
                                                               tGCGCCAGACGTTTGTCGTCGATAACTACCACCACCACCCGCGACGGTAGTATGCAGCCGTTAATGGCCTAATCGATAGAGCCAAGGCCCATCGGACCTGCACACGCCAGCAGTGCACTATTGCCGCCAGGTTTGACGcc                                                                     >  2:17845/1‑140 (MQ=255)
                                                                   ccAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATg                                                                 <  1:833852/140‑1 (MQ=255)
                                                                      gACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGCTAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGa                                                              <  2:92723/140‑1 (MQ=255)
                                                                      gACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGa                                                              <  1:968975/140‑1 (MQ=255)
                                                                      gACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGa                                                              <  2:1644467/140‑1 (MQ=255)
                                                                      gACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGa                                                              <  1:968667/140‑1 (MQ=255)
                                                                      gACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGa                                                              <  1:845832/140‑1 (MQ=255)
                                                                      gACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGa                                                              <  1:1438295/140‑1 (MQ=255)
                                                                      gACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGa                                                              <  1:838159/140‑1 (MQ=255)
                                                                      gACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGa                                                              <  1:1081951/140‑1 (MQ=255)
                                                                        cGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATg                                                            <  1:966974/140‑1 (MQ=255)
                                                                        cGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATg                                                            <  1:572698/140‑1 (MQ=255)
                                                                        cGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATg                                                            <  1:572588/140‑1 (MQ=255)
                                                                        cGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATAGTGCCGCCAGGTTTGACGCCCATGCGATg                                                            <  1:577927/140‑1 (MQ=255)
                                                                        cGTTTGTCGTCGATATCGACCACATCCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATg                                                            <  1:1640836/140‑1 (MQ=255)
                                                                          tttGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCt                                                          <  1:585520/140‑1 (MQ=255)
                                                                          cttctcgtcgAAATCGACCACCACCACCCGCGACGGTTGAATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCACATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCt                                                          <  2:1251755/136‑1 (MQ=255)
                                                                            tGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCa                                                        >  1:412728/1‑140 (MQ=255)
                                                                            tGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCa                                                        >  2:784263/1‑140 (MQ=255)
                                                                            tGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCa                                                        >  2:358654/1‑140 (MQ=255)
                                                                            tGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCa                                                        <  1:1273501/140‑1 (MQ=255)
                                                                            tGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCa                                                        >  2:1403360/1‑140 (MQ=255)
                                                                              tcgtcgATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATa                                                      <  1:507715/140‑1 (MQ=255)
                                                                              tcgtcgATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATa                                                      <  1:803865/140‑1 (MQ=255)
                                                                                  cgATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCGATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACa                                                  <  1:1389145/140‑1 (MQ=255)
                                                                                   gATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACat                                                 <  2:303241/140‑1 (MQ=255)
                                                                                         gACCACCACCCCCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                           <  1:1344214/140‑1 (MQ=255)
                                                                                         gACCACCACCCCCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                           <  2:644963/140‑1 (MQ=255)
                                                                                         gACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                           <  1:711904/140‑1 (MQ=255)
                                                                                         gACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                           <  1:4630/140‑1 (MQ=255)
                                                                                         gACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                           <  1:720427/140‑1 (MQ=255)
                                                                                         gACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                           <  1:1140811/140‑1 (MQ=255)
                                                                                         gACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCg                                           <  1:7641/140‑1 (MQ=255)
                                                                                          accaccaccaccCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCgt                                          <  1:170679/140‑1 (MQ=255)
                                                                                               caccaccCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGgtgt                                     >  1:539263/1‑140 (MQ=255)
                                                                                               caccaccCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGgtgt                                     >  2:676055/1‑140 (MQ=255)
                                                                                                    cccGCGACGGTTGTATGCCGCCGTTAAAGGCGAAATCGATAGCGCCAATGCCCATCGGACCTGCCCACGCCGGCAGTGCAATATAGCCGCCCGGTTTGACGCCCAAGCGATGCTCATAAACATATTGCGTGGGGTGATaa                                >  2:990104/1‑140 (MQ=255)
                                                                                                     ccGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAAt                               >  2:1342769/1‑140 (MQ=255)
                                                                                                        cgACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCTGGTTTGACGCCCATGCGATGCTCATAAACAGATTGCGTGGGGTGATAATTgg                            >  2:1685076/1‑140 (MQ=255)
                                                                                                          aaggTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCa                          <  2:837914/138‑1 (MQ=255)
                                                                                                          aCGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCa                          <  1:316555/140‑1 (MQ=255)
                                                                                                                  aTGCCGCCGTTCATGGCGTTATAGATCGCGCCAATGCCCATCGGACCGGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGc                  <  1:469675/140‑1 (MQ=255)
                                                                                                                  aTGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGc                  <  1:987857/140‑1 (MQ=255)
                                                                                                                  aTGCCGCCGTTAATGGCGTAATAGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGc                  <  1:1421030/140‑1 (MQ=255)
                                                                                                                  aTGCCGCCGTTAATGGCGTAATAGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGc                  <  1:1420727/140‑1 (MQ=255)
                                                                                                                    gccgccGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCAc                <  1:1170478/140‑1 (MQ=255)
                                                                                                                       gccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaa             <  2:1093034/140‑1 (MQ=255)
                                                                                                                        ccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaat            >  1:1111329/1‑140 (MQ=255)
                                                                                                                        ccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaat            >  1:1357582/1‑140 (MQ=255)
                                                                                                                        ccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaat            >  2:825595/1‑140 (MQ=255)
                                                                                                                        ccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaat            >  1:6616/1‑140 (MQ=255)
                                                                                                                        ccgTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCaat            >  2:1238419/1‑140 (MQ=255)
                                                                                                                            ttaTGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATg        <  1:978364/138‑1 (MQ=255)
                                                                                                                            tAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATg        <  1:1262580/140‑1 (MQ=255)
                                                                                                                            tAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATg        <  1:474858/140‑1 (MQ=255)
                                                                                                                            tAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATg        <  1:978390/140‑1 (MQ=255)
                                                                                                                             aaTGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgc       <  1:64531/140‑1 (MQ=255)
                                                                                                                              aTGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATgca      <  1:1337785/140‑1 (MQ=255)
                                                                                                                                  cGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCTGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACAGATTGCGTGGTGTGATCATTGGCATGATAAGCACCAATAATGCAGcac  >  2:291497/1‑140 (MQ=255)
                                                                                                                                  cGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGcac  >  2:528175/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TTCACATACACCAGCTCAATGCCTTTACTGGCCGCCAGTTCCACCGGCAGCAGCTTCTGTACCTGCGCCAGACGTTTGTCGTCGATATCGACCACCACCACCCGCGACGGTTGTATGCCGCCGTTAATGGCGTAATCGATAGCGCCAATGCCCATCGGACCTGCACACGCCAGCAGTGCAATATTGCCGCCAGGTTTGACGCCCATGCGATGCTCATAAACATATTGCGTGGTGTGATAATTGGCATGATAAGCACCAATAATGCAGCAC  >  NC_000913/3076744‑3077013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: