BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	423,539	T→G	13.0%	L341R (CTG→CGG)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	423,539	0	T	G	13.0%	224.8 / 5.6	104	L341R (CTG→CGG)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand): ref base T (41/47); new base G (2/14); total (43/61)
Fisher's exact test for biased strand distribution p-value = 1.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATG  >  NC_000913/423403‑423673

gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTggg                                                                                                                                     >  2:1443859/1‑140 (MQ=255)

gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTggg                                                                                                                                     >  1:322291/1‑140 (MQ=255)

gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTggg                                                                                                                                     >  2:1630240/1‑140 (MQ=255)

gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTggg                                                                                                                                     >  1:1351336/1‑140 (MQ=255)

     cTTGCCGATGCTGTATTATCAGGCGGAAAGTCTGGTGAATTAAATTTATCGCGGGGAAGACATTATGGACCGCCACTGGCTGAAAGCGCCGTGTAATTTGGAGGTCGGGCGGCTGTATGTGGTCAATATGCTGGGGGAgg                                                                                                                                <  2:378641/140‑1 (MQ=255)

     cTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAgg                                                                                                                                >  1:1124933/1‑140 (MQ=255)

     cTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAgg                                                                                                                                >  2:915678/1‑140 (MQ=255)

         ccGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCggg                                                                                                                            >  1:658775/1‑140 (MQ=255)

             agatgttttATCTGTGGTAATGTCTGGTGAATGAAATTTATCGCGGGTATGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGATTTTGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTggg                                                                                                                        <  2:1472499/133‑1 (MQ=255)

             aGCTGGATTATCTTTCGGAAAGTCTGGTGATTGAATTTTATCGCGGGGATGACTGTATTGTCCGCCACTGGCTTAAAGCGCCGTGGATTATGGACGGCTGGCGGCTGGATGTGTTGCATATGCTGGGGGAGGCGGGTggg                                                                                                                        <  2:273634/140‑1 (MQ=255)

                tGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgcg                                                                                                                     <  1:232619/140‑1 (MQ=255)

                  gtttATCTGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAACGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGGGGGTGGGgcgcg                                                                                                                   <  2:1023205/138‑1 (MQ=255)

                  gcttATCAGTCGGAAAGTCTGGTGAATTATATTTTTCGCGGGGAAGACAGTTTTGTCCGCCTCTGGCTGATAGCGCCGTGGTATATGTACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgcgcg                                                                                                                   <  2:46266/138‑1 (MQ=255)

                  gATTATCTGTCGTAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACTGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATTTGCTGGGGGAGGCGGGTGGGgcgcg                                                                                                                   <  1:1145/140‑1 (MQ=255)

                  gATTATCTGTCGGAATGTGTGGTGATTTAATTTTATCGGGGGGACGACAGTTTTGTCGGCCACTGGCTGAAAGGGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgcgcg                                                                                                                   <  1:22028/140‑1 (MQ=255)

                  gATTATCAGTCGTAAAGTCTGGTGAATGAAATTTATCGCGGGTAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgcgcg                                                                                                                   <  2:1460241/140‑1 (MQ=255)

                  gATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgcgcg                                                                                                                   <  2:1166842/140‑1 (MQ=255)

                  gATTAACAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGACGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGTTGTGGGGCATATGCTGGGGGAGGCGGGTGGGgcgcg                                                                                                                   <  2:1023322/140‑1 (MQ=255)

                  cataaTCAGTCGGAAAGTCTGGTGATTGAAATTTATCGCGGGGAAGACTTTTTTGTCCGCCCCTGGGTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGGGCATATGGGGGGGGAGGCGGGTGGGgcgcg                                                                                                                   <  2:1583083/136‑1 (MQ=255)

                     tATCAGTCGGAAAGTCTGGTTAATGAAATTTATCGCGGGGAAGAAAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGTAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaa                                                                                                                <  2:309323/140‑1 (MQ=255)

                     tATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAATACATTATTGTCCGCCACTGGTTGAAAGCGCCGTGGAATATGGAGGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGGGGGTGGGGCGCGCaa                                                                                                                <  2:309252/140‑1 (MQ=255)

                       tccgTCGGAAAGTCTGGTGAATGAATTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATAGGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGCGGCGGGTGGGGCGCGCaata                                                                                                              <  1:1455387/137‑1 (MQ=255)

                       taaGTCGTAAAGTCTGGTTAATTAAATTTATCGGGGGGAAGAAAGTATTGTCCGCAACTGGCTCAAAGCGCCTTGTAATATGGACGGCTGGCGGCTGGATGTGGTGAATATGCTGGGGTTGGGGGGTGGGGCGCGCaata                                                                                                              <  2:1251470/138‑1 (MQ=255)

                       tCAGTGGGAAAGTCTGGTGAATGAAATTTTTCGCGGGGAAGACTGTTTTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaata                                                                                                              <  2:90114/140‑1 (MQ=255)

                       tCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCtata                                                                                                              >  2:1683411/1‑140 (MQ=255)

                         aGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaataat                                                                                                            >  2:1549557/1‑140 (MQ=255)

                            cGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAATACAGTATGGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGCCGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGGGGGTGGGGCGCGCAATAATATg                                                                                                         <  1:1532066/140‑1 (MQ=255)

                            cGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCACTAATATg                                                                                                         >  2:137589/1‑140 (MQ=255)

                            cGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATg                                                                                                         >  1:112516/1‑140 (MQ=255)

                              gaacgacTGGTGACTGACATTTATCGCGGGGACGACAGCATTGTCCGCCAGGGGCTGAACGCGCCGTGGATAATGGCCGCCTGGCGGCTGGATGTGGTGCATATGCTGGGGGCGGCGGGTGGGGCGCGCAATAATATgca                                                                                                       <  2:216423/134‑1 (MQ=255)

                              gAATGTCTGGTGAATTAAATTTATCGCGGGTAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgca                                                                                                       <  2:216361/140‑1 (MQ=255)

                                 tgTCTGGTTATTTAATTTTATCGCGGGGAAGACTTTTTTGTCCGCAACTGGCTGAAAGCGCCGTGGATTATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgcagca                                                                                                    <  2:847428/139‑1 (MQ=255)

                                 tgTCTGGTGAATGAAATTTATCGGGGGGATGCCAGTATTGTCCGCCACTGGCTGAAAGCGCCGGGGAATATGGACGGCGGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATgcagca                                                                                                    <  2:1245136/139‑1 (MQ=255)

                                 aGTCTGTTGAATGAATTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGATAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgcagca                                                                                                    <  2:874729/140‑1 (MQ=255)

                                 aGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgcagca                                                                                                    <  2:872625/140‑1 (MQ=255)

                                     tGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGtt                                                                                                >  2:732018/1‑140 (MQ=255)

                                       gTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGc                                                                                              >  2:942405/1‑140 (MQ=255)

                                                    aTCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGaa                                                                                 >  1:908954/1‑140 (MQ=255)

                                                        cgGGGATGACAGTTTTGTCCGCCAGTGGCTGAAAGCGCGGTGGAATTTGGACGGCTGGCGGCTGGATGTGGTGCATTTGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcgg                                                                             <  2:1324333/140‑1 (MQ=255)

                                                         ggggAAGACTGTTTTGTCCGCCACTGGCTGAAAGCGCCGTGGATTATGGAGGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcggc                                                                            <  2:944270/140‑1 (MQ=255)

                                                         ggggAAGACAGTATTGTCCGCCACGGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcggc                                                                            <  2:944188/140‑1 (MQ=255)

                                                                  tgTATTGTCCGCCAGTGGCTGACTGCGCCGTGGCAGTTGGCCGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAAc                                                                   <  2:774713/139‑1 (MQ=255)

                                                                    ttttGTCCGCAACTGGCTGATAGCGCCGTGGAATATGGAGGGCTGGCGGGTGGATGTGGTGCATATGCTGGGGGTGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAAccc                                                                 <  2:1202533/138‑1 (MQ=255)

                                                                    tATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATTTGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAAccc                                                                 <  2:1519011/140‑1 (MQ=255)

                                                                       tGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGc                                                              >  2:946171/1‑140 (MQ=255)

                                                                       tGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGc                                                              >  2:946672/1‑140 (MQ=255)

                                                                         tCCGCCACTGGCTTAAAGGGCAGTGGAATATGGACGGCTGGGGGCTGGATGTGGTGAATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCGGGGATCACCGAAGCGGCGAAAGAAACCCAGCCg                                                            <  2:1214105/140‑1 (MQ=255)

                                                                         tCCGCCACTGGCTGACTGCGCCGTGGTCTATGGCCGGCTGGCGGATGGATGTGGGGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGTAAGAAACCCAGCCg                                                            <  2:778985/140‑1 (MQ=255)

                                                                         tCCGCCACTGGCTGAACGCGCGGTGGAATTTGGCCGGCTGGCGGCTGGTTGTGGTGCATATGCTGGGGGTGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCg                                                            <  2:1519350/140‑1 (MQ=255)

                                                                         tCCGCCACTGGCTGAACGCGCCGTGGAATATGGACGGCTGGGGGCTGGATGTGGTGCATTTGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCg                                                            <  2:1213892/140‑1 (MQ=255)

                                                                         tCCGCCACTGGCTGAAAGCGCCGTGTAATATGGACGGCTGGCGGCTGGATGTGGTGAATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCg                                                            <  1:2553/140‑1 (MQ=255)

                                                                         tCCGCCACTGGCTGAAAGCGCCGTGGAATTTGGCCGGCTGGCGGCTGGATGTGGGGCATATGATGGGGGTGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCg                                                            <  2:769760/140‑1 (MQ=255)

                                                                         tCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCg                                                            >  1:1480275/1‑140 (MQ=255)

                                                                         tCCGCCACTGGCTGAAAGCGCCGTGGAATAGGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCg                                                            <  1:852424/140‑1 (MQ=255)

                                                                           cGCCCCTGGCTGAAAGCGCCGTGGAATTTGGAGGGCTGGCGGTTGGATGTGGTGCATTTGCTGGGGGAGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGa                                                          <  2:544191/140‑1 (MQ=255)

                                                                           cGCCACTGGCTGAACGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGa                                                          <  2:1016382/140‑1 (MQ=255)

                                                                           cGCCACTGGCTGAAAGCGCCGTGTAATATGGCCGGCTGGGGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGa                                                          <  1:1331966/140‑1 (MQ=255)

                                                                           cGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGa                                                          >  1:399351/1‑140 (MQ=255)

                                                                           cGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGa                                                          <  2:656191/140‑1 (MQ=255)

                                                                            gCCACTGGCTGAACGCGCCGTGTAATTTGTCGGGGTGGCGGCTGGTTGTGGTGCTTATGGTGGGGGTGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGaa                                                         <  1:675500/140‑1 (MQ=255)

                                                                            gCCACGGGCTGAATGGGCCGTGTAATATGGAGGGCTGGCGGCTGGATTTGGTGCATTTGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGaa                                                         <  1:765147/140‑1 (MQ=255)

                                                                              cACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCCTATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGc                                                       >  2:796995/1‑140 (MQ=255)

                                                                              cACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGc                                                       >  2:852435/1‑140 (MQ=255)

                                                                              cACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGc                                                       >  1:1141503/1‑140 (MQ=255)

                                                                              cACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGc                                                       >  2:648464/1‑140 (MQ=255)

                                                                              cACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGc                                                       >  2:638856/1‑140 (MQ=255)

                                                                              cACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGc                                                       >  2:638800/1‑140 (MQ=255)

                                                                              cACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGc                                                       >  2:1302263/1‑140 (MQ=255)

                                                                              cACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAAGATGCCGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGc                                                       >  2:1300077/1‑140 (MQ=255)

                                                                              cACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGAGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGc                                                       >  1:279041/1‑140 (MQ=255)

                                                                                   gCTGAAAGCGCCTTGGAATATGGACGGCTGGCGGCTGGAGTTGGTGCATATGGTGGGGGAGGCGGGTGGGGCGCGCAATAATCTGCAGAACGTTGCCGGGATCACCGAAGCGGCGAACGAAACCCAGCCGGAAGCGtata                                                  >  2:761951/1‑140 (MQ=255)

                                                                                   gCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGtata                                                  >  1:957650/1‑140 (MQ=255)

                                                                                   gCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGtata                                                  >  1:1665475/1‑140 (MQ=255)

                                                                                   gCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGACGCGGCGAAAGAAACCCAGCCGGAAGCGtata                                                  >  2:183925/1‑140 (MQ=255)

                                                                                   gCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGtaaa                                                  >  2:1635944/1‑138 (MQ=255)

                                                                                             ccGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGa                                        >  1:593684/1‑140 (MQ=255)

                                                                                                           gggttggcggctggATGTGGTGATTTTGGTGGGGGTGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATg                          <  1:1683411/136‑1 (MQ=255)

                                                                                                             gctggcggctgtATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCa                        <  1:90337/140‑1 (MQ=255)

                                                                                                             gctggcggctggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCa                        >  2:1025175/1‑140 (MQ=255)

                                                                                                               tggcggctgtTTGTGGTGCATATGCGGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACg                      <  1:850084/140‑1 (MQ=255)

                                                                                                               tggcggctggATGTGGTGCAAATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACg                      <  1:893035/140‑1 (MQ=255)

                                                                                                               tggcggctggAAGTGGTGCATATGCGGGGGGAGGCGCGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTAGGCGAACTTTTTGGCGATGCACg                      <  1:850121/140‑1 (MQ=255)

                                                                                                               gggcggctggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACg                      <  2:1282752/139‑1 (MQ=255)

                                                                                                               gggcggctggATGTGGTGCATATGCGGGGGGCGGCGGGGGGGGCGCGCAATAATCTGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACg                      <  1:539032/139‑1 (MQ=255)

                                                                                                                 gcggctggATGTGGTGCATTTGCGGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTACCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGc                    <  2:427725/140‑1 (MQ=255)

                                                                                                                 gcggctggATGTGGTGCATCTGCTGGGGGAGGCGGGTGGGGCGCGCACTAATATGCAGCACGTTACCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGc                    <  2:427694/140‑1 (MQ=255)

                                                                                                                 gcggctggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGc                    >  2:1308741/1‑140 (MQ=255)

                                                                                                                   ggctggTTGTGGTGCATTTGCGGGGGGTGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCaa                  <  1:1546586/140‑1 (MQ=255)

                                                                                                                   ggctggATGTGGTGAATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCaa                  <  1:1508774/140‑1 (MQ=255)

                                                                                                                    gctggATGTGGTGCATATGCGGGGGGAGGCGGGTGGCGCGCGCAAAAATACGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCCGCCGGAAGCGTATATTGACGGCGAACATTTTGGCGATGCACGGCAAt                 <  2:1485671/140‑1 (MQ=255)

                                                                                                                    gatggATGTGGTGAATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCTCCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATCTTGTCGGCGAACATTTTGGCGATGCACGGCAAt                 <  2:360134/138‑1 (MQ=255)

                                                                                                                        gATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGtt             >  1:1174414/1‑140 (MQ=255)

                                                                                                                              gtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGc       >  1:202722/1‑140 (MQ=255)

                                                                                                                              gtgCATATGCGGGGGGTGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGc       <  2:728791/140‑1 (MQ=255)

                                                                                                                              gtgCATATGCGGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGc       <  1:817451/140‑1 (MQ=255)

                                                                                                                               tgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGcc      >  2:399619/1‑140 (MQ=255)

                                                                                                                                  atatGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAt   >  2:362355/1‑140 (MQ=255)

                                                                                                                                  atatGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAt   >  2:1116219/1‑140 (MQ=255)

                                                                                                                                   tatGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCATTGGTTACAGGCCGAtg  <  2:920203/140‑1 (MQ=255)

                                                                                                                                   tatGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAtg  >  2:554627/1‑140 (MQ=255)

GCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATG  >  NC_000913/423403‑423673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`