Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,241,102 C→T 5.5% L387L (CTG→TTG)  sstT → sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,241,1020CT 5.5% 258.2 / 3.9 73L387L (CTG→TTG) sstTsodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base C (35/34);  new base T (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGG  >  NC_000913/3240978‑3241240
                                                                                                                            |                                                                                                                                          
gggggggTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTg                                                                                                                             <  1:831076/140‑1 (MQ=255)
    gggTCTCTGCTGCTGATTCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGt                                                                                                                         >  2:1274752/1‑140 (MQ=255)
    gggTCTCTGCTGCTGATTCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGt                                                                                                                         >  2:1274810/1‑140 (MQ=255)
      gTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGc                                                                                                                       >  1:212540/1‑140 (MQ=255)
      gTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGc                                                                                                                       >  1:212782/1‑140 (MQ=255)
         tctGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGt                                                                                                                    >  1:816686/1‑140 (MQ=255)
          ctgctgctgATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGtt                                                                                                                   <  2:316158/140‑1 (MQ=255)
                   aTCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTg                                                                                                                        >  2:1191436/1‑126 (MQ=255)
                   aTCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTg                                                                                                                        >  2:1191484/1‑126 (MQ=255)
                   aTCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTg                                                                                                                        <  1:1191436/126‑1 (MQ=255)
                   aTCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTg                                                                                                                        <  1:1191484/126‑1 (MQ=255)
                    tCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCACCTGACGTGCTGTTCACTGCGGCa                                                                                                         <  2:150421/140‑1 (MQ=255)
                    tCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCa                                                                                                         <  2:150420/140‑1 (MQ=255)
                    tCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCa                                                                                                         <  1:426387/140‑1 (MQ=255)
                     cccACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAg                                                                                                        >  2:1030744/1‑140 (MQ=255)
                              cTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAgg                                                                                               <  1:433653/140‑1 (MQ=255)
                               tGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGc                                                                                              >  1:775786/1‑140 (MQ=255)
                                      tggtCGGTATGTCGAACGTTATCGCCTTGCAGGTGGTTGCCGTCGGCTGTCTCATCGGCGTATTGCAGGACGCTTGCGTAACCGCGCTGAACTCTTCAGCTGACGTGCTGTTCACTGCGGAGGCTTGCCAGGCCGAAGAc                                                                                       >  2:322345/4‑140 (MQ=255)
                                      tGTTCGGTATTTCGCCCGATATCGCCAGGCAGGTGGTTGCCGTCGGCTGTATCAGCGGCGTATTGCAGGACTCTTGCGACACCGCGCTGTACTCTTCAACTGACGTGCTGTTCACGGCGGCCGCTTGCCAGGCCGAAGAc                                                                                       >  2:322309/1‑140 (MQ=255)
                                      tGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCGGTCGTCTTTTTCATCGGCGTATTGAAGGTCTCTTGCGAAACAGCGCTGAAGTCTTCAAGTGACGTGCTGTTCTCTGCGGCAGCTTGCGAGGCggacgag                                                                                       >  2:1495296/1‑139 (MQ=255)
                                      tGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCGGGACTCTTGCGTAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCGTGCCAGGCAgacgac                                                                                       >  2:171839/1‑140 (MQ=255)
                                      tGTTCGGTATTTCGAACGATAGCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGCACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGAc                                                                                       >  2:850809/1‑140 (MQ=255)
                                           ggTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCATCTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAATACGATCg                                                                                  >  1:262733/1‑140 (MQ=255)
                                           ggTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCg                                                                                  >  1:94298/1‑140 (MQ=255)
                                            gTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGt                                                                                 <  1:456585/140‑1 (MQ=255)
                                                 tCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGc                                                                            <  1:387288/140‑1 (MQ=255)
                                                 tCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGc                                                                            <  1:389716/140‑1 (MQ=255)
                                                 tCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGc                                                                            <  1:147977/140‑1 (MQ=255)
                                                     aCGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAAt                                                                        >  2:1432525/1‑140 (MQ=255)
                                                      cGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATa                                                                       <  2:1353405/140‑1 (MQ=255)
                                                           tCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGcc                                                                  <  1:1145419/140‑1 (MQ=255)
                                                           tCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGcc                                                                  <  1:420605/140‑1 (MQ=255)
                                                              ccATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTg                                                               <  2:770482/140‑1 (MQ=255)
                                                               cATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGc                                                              <  2:942882/140‑1 (MQ=255)
                                                               cATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGc                                                              <  1:355216/140‑1 (MQ=255)
                                                                 tGCATGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGt                                                            >  1:560980/1‑140 (MQ=255)
                                                                 tGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGTTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGt                                                            >  1:5753/1‑140 (MQ=255)
                                                                 tGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGTTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGt                                                            >  1:5735/1‑140 (MQ=255)
                                                                      gtggtTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGACACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGAGGCAGCTTTCCCGGCAGAAGACGATCGTCTGGCAAAGAGCGCCCTGCGaaatta                                                       >  2:899885/1‑140 (MQ=255)
                                                                      gtggtTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGtaatta                                                       >  2:899924/1‑140 (MQ=255)
                                                                       tggtTGCCGTCGGCTTTATCATCGGCGTATTGCAGGTCTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGtaattaa                                                      <  2:1044855/140‑1 (MQ=255)
                                                                       tggtTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGTTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGtaattaa                                                      <  2:5753/140‑1 (MQ=255)
                                                                       tggtTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGTTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGtaattaa                                                      <  2:5735/140‑1 (MQ=255)
                                                                       tggtTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGtaattaa                                                      <  1:1575732/140‑1 (MQ=255)
                                                                       tggcTGCCCTCGGCATTACCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGtaattaa                                                      <  2:87736/140‑1 (MQ=255)
                                                                       aggtTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGtaattaa                                                      <  2:267849/139‑1 (MQ=255)
                                                                               gTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTaa                                              >  1:118805/1‑140 (MQ=255)
                                                                                tCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAAc                                             >  2:367585/1‑140 (MQ=255)
                                                                                tCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAAc                                             >  1:567045/1‑140 (MQ=255)
                                                                                     tttATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCtt                                        <  2:623007/140‑1 (MQ=255)
                                                                                     tttATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCtt                                        <  2:690265/140‑1 (MQ=255)
                                                                                       tATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTc                                      >  2:1060847/1‑140 (MQ=255)
                                                                                              ggCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGTAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTAcg                               >  2:317247/1‑140 (MQ=255)
                                                                                              ggCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGTAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTAcg                               >  2:317541/1‑140 (MQ=255)
                                                                                              ggCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAATACGATCGTCTGGCAAATATCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTAcg                               >  2:1026550/1‑140 (MQ=255)
                                                                                              ggCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTAcg                               >  2:1026728/1‑140 (MQ=255)
                                                                                              ggCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTAcg                               >  2:407069/1‑140 (MQ=255)
                                                                                              ggCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTAcg                               >  2:1026665/1‑140 (MQ=255)
                                                                                                  tATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTAcggcgg                           <  2:933863/140‑1 (MQ=255)
                                                                                                  tATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTAcggcgg                           <  2:933505/140‑1 (MQ=255)
                                                                                                   aTTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGa                          >  1:146896/1‑140 (MQ=255)
                                                                                                         ggACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGt                    >  1:409237/1‑140 (MQ=255)
                                                                                                          gACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGtt                   >  2:867833/1‑140 (MQ=255)
                                                                                                              ctTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGTCGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTtct               <  1:912322/140‑1 (MQ=255)
                                                                                                              ctTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTtct               <  1:1154890/140‑1 (MQ=255)
                                                                                                              ctTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTtct               <  2:723711/140‑1 (MQ=255)
                                                                                                              ctTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTtct               <  2:122088/140‑1 (MQ=255)
                                                                                                               ttGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTtctc              >  1:1508373/1‑140 (MQ=255)
                                                                                                                    aaaCAGCGCTGAACTCTTAAAATTAAATTCAGATCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACtt         >  2:1403900/1‑140 (MQ=255)
                                                                                                                      aCCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTa       <  2:802850/140‑1 (MQ=255)
                                                                                                                      aCCGCGCTGAACTCTTCAACTGACGAGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGATTAACCACTATCGTCTACGGCGGAAGGGGTTTTCTCAACTTTa       <  2:613193/140‑1 (MQ=255)
                                                                                                                        cgcgCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTaaa     <  2:906235/140‑1 (MQ=255)
                                                                                                                        cgcgCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTaaa     <  1:634188/140‑1 (MQ=255)
                                                                                                                           gcTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCATATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACgg  <  1:565963/140‑1 (MQ=255)
                                                                                                                            |                                                                                                                                          
GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGG  >  NC_000913/3240978‑3241240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: