Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,279,147 G→T 5.1% L78F (TTG→TTT kbaZ → tagatose 6‑phosphate aldolase 1, kbaZ subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,279,1470GT 5.1% 286.3 / 2.2 78L78F (TTG→TTTkbaZtagatose 6‑phosphate aldolase 1, kbaZ subunit
Reads supporting (aligned to +/- strand):  ref base G (27/47);  new base T (2/2);  total (29/49)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

CTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGAT  >  NC_000913/3279008‑3279274
                                                                                                                                           |                                                                                                                               
cTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTGATACCGGAATGACGCCCGCCGATTTTCGCGGCTGTGTTTGTCAGCTCGCCGACTCGTTg                                                                                                                                 >  2:421108/1‑140 (MQ=255)
   cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAAt                                                                                                                              >  2:1214527/1‑140 (MQ=255)
   cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAAt                                                                                                                              >  1:1198964/1‑140 (MQ=255)
   cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAAt                                                                                                                              >  1:638845/1‑140 (MQ=255)
             cGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAgg                                                                                                                    >  1:1687282/1‑140 (MQ=255)
              gCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGa                                                                                                                   <  1:871194/140‑1 (MQ=255)
              gCCACTGCAAACCAAACGCCCTTACTCATTGAAGCAACCTCCAAACACGTAGACCAGTTCGCCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCACGa                                                                                                                   <  2:792329/140‑1 (MQ=255)
                cAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATg                                                                                                                 >  1:817677/1‑140 (MQ=255)
                  gTGCAAACCAATCGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTGTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCg                                                                                                               >  2:1175176/1‑140 (MQ=255)
                  gTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCg                                                                                                               >  1:1252156/1‑140 (MQ=255)
                  gTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCg                                                                                                               >  1:961713/1‑140 (MQ=255)
                   tGCAAACCAAACGCCGTTAATGATTGAAGCAAACTCCAATCAGTTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTAAGCGGCTTAGTTTGTCAGCACGCCGACTCGATGAATTTCCCGCAGGATGCGt                                                                                                              >  2:287002/1‑140 (MQ=255)
                   tGCAAACCAAACGCCGATACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGCTGCGt                                                                                                              >  2:287230/1‑140 (MQ=255)
                     caaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTg                                                                                                            <  1:883307/140‑1 (MQ=255)
                     caaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTg                                                                                                            <  1:883314/140‑1 (MQ=255)
                        accaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGAtt                                                                                                         >  1:1476891/1‑140 (MQ=255)
                        accaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGAtt                                                                                                         >  1:1624613/1‑140 (MQ=255)
                        accaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGAtt                                                                                                         <  2:1465656/140‑1 (MQ=255)
                        accaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGAtt                                                                                                         >  2:1525276/1‑140 (MQ=255)
                          caaacGCCGGTACTGATTGAAGCAACCTCCAAGCAGCTAGACCAGTTCGGCGGTGATACCGGACTGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCGGGATGCCTGGATTCt                                                                                                       >  2:1219686/1‑140 (MQ=255)
                                  gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGgtggtg                                                                                               <  2:320104/140‑1 (MQ=255)
                                  gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGgtggtg                                                                                               <  2:323174/140‑1 (MQ=255)
                                  cttACTGATTGAATCAACCAACAACAAGTAAGACCTGTTCGTCGGTTATACCGGCCAGACTCCCGCCGATTTTCGCGGATATGTTTGACAGCTCGCCGACTCGTTGAATTTCCCGCACGATGCGATGATTCTGGgtggtg                                                                                               <  2:1644444/139‑1 (MQ=255)
                                    tacagcctGAAGCAACCACCAATCAGGTAGACCAGTTCGGCGCTTATACCGGTATGACGCCCGCAGTTTTTCGCGGCTTTGTTTGTCAGATCGCCGAATCGTTGAATTTCCCGCAGGATTCGTTGATTCTGGGTGGTGAc                                                                                             <  1:1656350/133‑1 (MQ=255)
                                    tACTGATTGAAGCTAGCTCCAATCAGGTAGACCATTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAAGTTCCCGCAGGATGCGTTGATTCTGGGTGGTGAc                                                                                             <  2:381880/140‑1 (MQ=255)
                                    tACTGATTGAAGCAACCTCCCTTCAGGTAGACCAGTTCGGCGGTGATACCGGACTGACGCCCGCCGAATTGCGCGGCTTTGTTTGTCAGCTCGCCGACTCCTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGAc                                                                                             <  2:381933/140‑1 (MQ=255)
                                    tACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTTAATTTCCCGCAGTATGCGTTGATTCTGGGTGGTGAc                                                                                             >  2:1316254/1‑140 (MQ=255)
                                    tACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTTAATTTCCCGCAGTATGCGTTGATTCTGGGTGGTGAc                                                                                             >  2:659380/1‑140 (MQ=255)
                                    tACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACACGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGAc                                                                                             <  2:1514792/140‑1 (MQ=255)
                                        gATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATc                                                                                         <  1:955622/140‑1 (MQ=255)
                                         aTTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATGCTGGGTGGTGACCATCt                                                                                        >  1:257734/1‑140 (MQ=255)
                                           tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTgg                                                                                      >  2:302393/1‑140 (MQ=255)
                                           tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTgg                                                                                      >  1:515033/1‑140 (MQ=255)
                                                     tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCg                                                                            <  1:1011753/140‑1 (MQ=255)
                                                            aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCaa                                                                     <  1:1014336/140‑1 (MQ=255)
                                                            aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCaa                                                                     <  1:1521983/140‑1 (MQ=255)
                                                            aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTATGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCaa                                                                     <  1:526144/140‑1 (MQ=255)
                                                                     aGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAActgcccg                                                            <  1:1367911/140‑7 (MQ=255)
                                                                      gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccgg                                                           <  1:1083393/140‑1 (MQ=255)
                                                                      gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccgg                                                           <  1:232653/140‑1 (MQ=255)
                                                                      gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGAGTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccgg                                                           >  2:241794/1‑140 (MQ=255)
                                                                      cgtCGGCGGGTCGACCGGCATGACGCCCGCCGCTTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccgg                                                           <  1:1083516/138‑1 (MQ=255)
                                                                       ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccggc                                                          <  1:36117/140‑1 (MQ=255)
                                                                       ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccggc                                                          <  1:36159/140‑1 (MQ=255)
                                                                       ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccggc                                                          <  1:36139/140‑1 (MQ=255)
                                                                       ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccggc                                                          <  1:36125/140‑1 (MQ=255)
                                                                       ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccggc                                                          <  1:1312801/140‑1 (MQ=255)
                                                                       ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGGGGTGTCCATCTGGGGCCAAACCGCTGGCAAAACCTgccggc                                                          >  1:1687913/1‑140 (MQ=255)
                                                                        tCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccggcc                                                         >  1:1169620/1‑140 (MQ=255)
                                                                            cgtttATAACGGAAAGACGCCCGCAGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCACGCAGGATGCGTTGTTTCTGGGTGGTGACCATCTGGGGCCAATCCGCTGGCAAAACCTGCCGGCCGCTc                                                     <  2:368246/137‑1 (MQ=255)
                                                                            cggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTc                                                     <  1:951114/140‑1 (MQ=255)
                                                                               ttATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAgg                                                  >  2:1278876/1‑140 (MQ=255)
                                                                                 ataCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCa                                                <  1:1534180/140‑1 (MQ=255)
                                                                                 ataCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCa                                                >  1:296211/1‑140 (MQ=255)
                                                                                 ataCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCa                                                <  1:755584/140‑1 (MQ=255)
                                                                                 ataCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCa                                                <  1:745835/140‑1 (MQ=255)
                                                                                 ataCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCa                                                <  1:744904/140‑1 (MQ=255)
                                                                                  taCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCaa                                               <  2:415652/140‑1 (MQ=255)
                                                                                  taCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCaa                                               <  2:1184158/140‑1 (MQ=255)
                                                                                  taCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCaa                                               <  2:1181565/140‑1 (MQ=255)
                                                                                      ggAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGc                                           <  1:837574/140‑1 (MQ=255)
                                                                                      ggAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGc                                           <  2:1070042/140‑1 (MQ=255)
                                                                                         aTGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCaa                                        >  1:607328/1‑140 (MQ=255)
                                                                                         aTGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCaa                                        >  1:607206/1‑140 (MQ=255)
                                                                                             cgcccgccGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGcc                                    >  1:756141/1‑140 (MQ=255)
                                                                                              gcccgccGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTTAATTTCCCGCAGTATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCg                                   <  1:1316254/140‑1 (MQ=255)
                                                                                              gcccgccGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTTAATTTCCCGCAGTATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCg                                   <  1:659380/140‑1 (MQ=255)
                                                                                                    cgATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGAtgatt                             <  2:1102984/140‑1 (MQ=255)
                                                                                                    cgATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGAtgatt                             <  2:1103484/140‑1 (MQ=255)
                                                                                                    cgATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGAtgatt                             <  2:163615/140‑1 (MQ=255)
                                                                                                    cgATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGAtgatt                             <  2:718900/140‑1 (MQ=255)
                                                                                                    cgATTTTCGCGGCTTTGTTTGACAGCTCGCCGACACTATGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGACATACCGCTGGCAAAACCTGCCGGCCGCTCTGCCAATGGCCAATGCCGAtgatt                             <  2:1500786/140‑1 (MQ=255)
                                                                                                         ttCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGAtgatttgatt                        <  1:1104149/140‑1 (MQ=255)
                                                                                                         ttCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGAtgatttgatt                        <  2:696522/140‑1 (MQ=255)
                                                                                                                 tttgtttgTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTa                <  2:690649/140‑1 (MQ=255)
                                                                                                                 cttgtttgtCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCTTAGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACATGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTa                <  2:377719/139‑1 (MQ=255)
                                                                                                                 attgtttgtCAGCTCGCCGACTCGTTGAATTTCCCTCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGTGCCAAACCTCTGGCAAAACCTGCCGGCCGATAAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCtt                <  2:377743/139‑2 (MQ=255)
                                                                                                                   tgtttgtCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACg              >  2:1578553/1‑140 (MQ=255)
                                                                                                                               cGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGAt  >  1:1153349/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                               
CTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGAT  >  NC_000913/3279008‑3279274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: