Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,279,160 G→T 5.2% D83Y (GAT→TAT)  kbaZ → tagatose 6‑phosphate aldolase 1, kbaZ subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,279,1600GT 5.2% 286.7 / 2.2 77D83Y (GAT→TAT) kbaZtagatose 6‑phosphate aldolase 1, kbaZ subunit
Reads supporting (aligned to +/- strand):  ref base G (24/49);  new base T (2/2);  total (26/51)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

CGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATT  >  NC_000913/3279021‑3279295
                                                                                                                                           |                                                                                                                                       
cGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAgg                                                                                                                                         >  1:1687282/1‑140 (MQ=255)
 gCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGa                                                                                                                                        <  1:871194/140‑1 (MQ=255)
 gCCACTGCAAACCAAACGCCCTTACTCATTGAAGCAACCTCCAAACACGTAGACCAGTTCGCCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCACGa                                                                                                                                        <  2:792329/140‑1 (MQ=255)
   cAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATg                                                                                                                                      >  1:817677/1‑140 (MQ=255)
     gTGCAAACCAATCGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTGTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCg                                                                                                                                    >  2:1175176/1‑140 (MQ=255)
     gTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCg                                                                                                                                    >  1:1252156/1‑140 (MQ=255)
     gTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCg                                                                                                                                    >  1:961713/1‑140 (MQ=255)
      tGCAAACCAAACGCCGTTAATGATTGAAGCAAACTCCAATCAGTTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTAAGCGGCTTAGTTTGTCAGCACGCCGACTCGATGAATTTCCCGCAGGATGCGt                                                                                                                                   >  2:287002/1‑140 (MQ=255)
      tGCAAACCAAACGCCGATACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGCTGCGt                                                                                                                                   >  2:287230/1‑140 (MQ=255)
        caaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTg                                                                                                                                 <  1:883314/140‑1 (MQ=255)
        caaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTg                                                                                                                                 <  1:883307/140‑1 (MQ=255)
           accaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGAtt                                                                                                                              >  1:1476891/1‑140 (MQ=255)
           accaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGAtt                                                                                                                              >  1:1624613/1‑140 (MQ=255)
           accaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGAtt                                                                                                                              >  2:1525276/1‑140 (MQ=255)
           accaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGAtt                                                                                                                              <  2:1465656/140‑1 (MQ=255)
             caaacGCCGGTACTGATTGAAGCAACCTCCAAGCAGCTAGACCAGTTCGGCGGTGATACCGGACTGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCGGGATGCCTGGATTCt                                                                                                                            >  2:1219686/1‑140 (MQ=255)
                     gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGgtggtg                                                                                                                    <  2:320104/140‑1 (MQ=255)
                     gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGgtggtg                                                                                                                    <  2:323174/140‑1 (MQ=255)
                     cttACTGATTGAATCAACCAACAACAAGTAAGACCTGTTCGTCGGTTATACCGGCCAGACTCCCGCCGATTTTCGCGGATATGTTTGACAGCTCGCCGACTCGTTGAATTTCCCGCACGATGCGATGATTCTGGgtggtg                                                                                                                    <  2:1644444/139‑1 (MQ=255)
                       tacagcctGAAGCAACCACCAATCAGGTAGACCAGTTCGGCGCTTATACCGGTATGACGCCCGCAGTTTTTCGCGGCTTTGTTTGTCAGATCGCCGAATCGTTGAATTTCCCGCAGGATTCGTTGATTCTGGGTGGTGAc                                                                                                                  <  1:1656350/133‑1 (MQ=255)
                       tACTGATTGAAGCTAGCTCCAATCAGGTAGACCATTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAAGTTCCCGCAGGATGCGTTGATTCTGGGTGGTGAc                                                                                                                  <  2:381880/140‑1 (MQ=255)
                       tACTGATTGAAGCAACCTCCCTTCAGGTAGACCAGTTCGGCGGTGATACCGGACTGACGCCCGCCGAATTGCGCGGCTTTGTTTGTCAGCTCGCCGACTCCTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGAc                                                                                                                  <  2:381933/140‑1 (MQ=255)
                       tACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTTAATTTCCCGCAGTATGCGTTGATTCTGGGTGGTGAc                                                                                                                  >  2:1316254/1‑140 (MQ=255)
                       tACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTTAATTTCCCGCAGTATGCGTTGATTCTGGGTGGTGAc                                                                                                                  >  2:659380/1‑140 (MQ=255)
                       tACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACACGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGAc                                                                                                                  <  2:1514792/140‑1 (MQ=255)
                           gATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATc                                                                                                              <  1:955622/140‑1 (MQ=255)
                            aTTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATGCTGGGTGGTGACCATCt                                                                                                             >  1:257734/1‑140 (MQ=255)
                              tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTgg                                                                                                           >  2:302393/1‑140 (MQ=255)
                              tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTgg                                                                                                           >  1:515033/1‑140 (MQ=255)
                                        tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCg                                                                                                 <  1:1011753/140‑1 (MQ=255)
                                               aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCaa                                                                                          <  1:1014336/140‑1 (MQ=255)
                                               aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCaa                                                                                          <  1:1521983/140‑1 (MQ=255)
                                               aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTATGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCaa                                                                                          <  1:526144/140‑1 (MQ=255)
                                                        aGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAActgcccg                                                                                 <  1:1367911/140‑7 (MQ=255)
                                                         gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccgg                                                                                <  1:1083393/140‑1 (MQ=255)
                                                         gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccgg                                                                                <  1:232653/140‑1 (MQ=255)
                                                         gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGAGTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccgg                                                                                >  2:241794/1‑140 (MQ=255)
                                                         cgtCGGCGGGTCGACCGGCATGACGCCCGCCGCTTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccgg                                                                                <  1:1083516/138‑1 (MQ=255)
                                                          ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccggc                                                                               <  1:1312801/140‑1 (MQ=255)
                                                          ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccggc                                                                               <  1:36117/140‑1 (MQ=255)
                                                          ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccggc                                                                               <  1:36159/140‑1 (MQ=255)
                                                          ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccggc                                                                               <  1:36125/140‑1 (MQ=255)
                                                          ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccggc                                                                               <  1:36139/140‑1 (MQ=255)
                                                          ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGGGGTGTCCATCTGGGGCCAAACCGCTGGCAAAACCTgccggc                                                                               >  1:1687913/1‑140 (MQ=255)
                                                           tCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTgccggcc                                                                              >  1:1169620/1‑140 (MQ=255)
                                                               cgtttATAACGGAAAGACGCCCGCAGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCACGCAGGATGCGTTGTTTCTGGGTGGTGACCATCTGGGGCCAATCCGCTGGCAAAACCTGCCGGCCGCTc                                                                          <  2:368246/137‑1 (MQ=255)
                                                               cggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTc                                                                          <  1:951114/140‑1 (MQ=255)
                                                                  ttATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAgg                                                                       >  2:1278876/1‑140 (MQ=255)
                                                                    ataCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCa                                                                     <  1:755584/140‑1 (MQ=255)
                                                                    ataCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCa                                                                     <  1:744904/140‑1 (MQ=255)
                                                                    ataCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCa                                                                     <  1:1534180/140‑1 (MQ=255)
                                                                    ataCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCa                                                                     <  1:745835/140‑1 (MQ=255)
                                                                    ataCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCa                                                                     >  1:296211/1‑140 (MQ=255)
                                                                     taCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCaa                                                                    <  2:415652/140‑1 (MQ=255)
                                                                     taCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCaa                                                                    <  2:1184158/140‑1 (MQ=255)
                                                                     taCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCaa                                                                    <  2:1181565/140‑1 (MQ=255)
                                                                         ggAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGc                                                                <  2:1070042/140‑1 (MQ=255)
                                                                         ggAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGc                                                                <  1:837574/140‑1 (MQ=255)
                                                                            aTGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCaa                                                             >  1:607206/1‑140 (MQ=255)
                                                                            aTGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCaa                                                             >  1:607328/1‑140 (MQ=255)
                                                                                cgcccgccGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGcc                                                         >  1:756141/1‑140 (MQ=255)
                                                                                 gcccgccGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTTAATTTCCCGCAGTATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCg                                                        <  1:1316254/140‑1 (MQ=255)
                                                                                 gcccgccGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTTAATTTCCCGCAGTATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCg                                                        <  1:659380/140‑1 (MQ=255)
                                                                                       cgATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGAtgatt                                                  <  2:1103484/140‑1 (MQ=255)
                                                                                       cgATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGAtgatt                                                  <  2:163615/140‑1 (MQ=255)
                                                                                       cgATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGAtgatt                                                  <  2:718900/140‑1 (MQ=255)
                                                                                       cgATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGAtgatt                                                  <  2:1102984/140‑1 (MQ=255)
                                                                                       cgATTTTCGCGGCTTTGTTTGACAGCTCGCCGACACTATGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGACATACCGCTGGCAAAACCTGCCGGCCGCTCTGCCAATGGCCAATGCCGAtgatt                                                  <  2:1500786/140‑1 (MQ=255)
                                                                                            ttCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGAtgatttgatt                                             <  1:1104149/140‑1 (MQ=255)
                                                                                            ttCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGAtgatttgatt                                             <  2:696522/140‑1 (MQ=255)
                                                                                                    tttgtttgTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTa                                     <  2:690649/140‑1 (MQ=255)
                                                                                                    cttgtttgtCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCTTAGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACATGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTa                                     <  2:377719/139‑1 (MQ=255)
                                                                                                    attgtttgtCAGCTCGCCGACTCGTTGAATTTCCCTCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGTGCCAAACCTCTGGCAAAACCTGCCGGCCGATAAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCtt                                     <  2:377743/139‑2 (MQ=255)
                                                                                                      tgtttgtCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACg                                   >  2:1578553/1‑140 (MQ=255)
                                                                                                                  cGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGAt                       >  1:1153349/1‑140 (MQ=255)
                                                                                                                                     ccGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGa    >  2:532147/1‑140 (MQ=255)
                                                                                                                                      cGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGAt   <  2:45447/140‑1 (MQ=255)
                                                                                                                                       gCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGAtt  <  2:708017/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
CGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATT  >  NC_000913/3279021‑3279295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: