Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,298,414 G→T 5.0% R145S (CGC→AGC)  yraR ← putative nucleoside‑diphosphate‑sugar epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,298,4140GT 5.0% 294.7 / 2.3 79R145S (CGC→AGC) yraRputative nucleoside‑diphosphate‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base G (30/45);  new base T (2/2);  total (32/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

ATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGTCGCCGC  >  NC_000913/3298275‑3298553
                                                                                                                                           |                                                                                                                                           
aTCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCg                                                                                                                                             >  2:342662/1‑140 (MQ=255)
    aCTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCa                                                                                                                                         <  2:852107/140‑1 (MQ=255)
     ctctGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCaa                                                                                                                                        >  2:1635652/1‑140 (MQ=255)
     ctctGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCaa                                                                                                                                        >  2:240137/1‑140 (MQ=255)
     ctctGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCaa                                                                                                                                        >  2:803197/1‑140 (MQ=255)
     ctctGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGCTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCaa                                                                                                                                        >  2:475303/1‑140 (MQ=255)
         gCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGt                                                                                                                                    <  2:591048/140‑1 (MQ=255)
               aTGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAAttt                                                                                                                              >  2:575897/1‑140 (MQ=255)
                   cccGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGc                                                                                                                          <  1:714379/140‑1 (MQ=255)
                       cgcCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGt                                                                                                                      >  1:1357645/1‑140 (MQ=255)
                        gcCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTGCATCCGCTGTTTGCGACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGtt                                                                                                                     >  1:1374528/1‑140 (MQ=255)
                            cGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTgg                                                                                                                 <  2:237957/140‑1 (MQ=255)
                                tctcGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGa                                                                                                             >  1:981454/1‑140 (MQ=255)
                                  tcGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGAtt                                                                                                           >  1:711299/1‑140 (MQ=255)
                                    gCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAACAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTaa                                                                                                         <  1:277419/140‑1 (MQ=255)
                                    gCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTaa                                                                                                         <  1:1360770/140‑1 (MQ=255)
                                    gCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTaa                                                                                                         <  1:1363538/140‑1 (MQ=255)
                                      aTCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATg                                                                                                       <  1:1325683/140‑1 (MQ=255)
                                      aTCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATg                                                                                                       <  1:1325715/140‑1 (MQ=255)
                                      aTCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATg                                                                                                       <  2:516545/140‑1 (MQ=255)
                                         aaTGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGctt                                                                                                    >  2:940149/1‑140 (MQ=255)
                                                ttCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCAt                                                                                             >  1:1575439/1‑140 (MQ=255)
                                                        aCCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCtt                                                                                     >  1:1394530/1‑140 (MQ=255)
                                                        aCCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCtt                                                                                     >  1:465974/1‑140 (MQ=255)
                                                           tGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGa                                                                                  <  2:697535/140‑1 (MQ=255)
                                                           tGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGa                                                                                  <  2:1423262/140‑1 (MQ=255)
                                                           tGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGa                                                                                  <  2:1009517/140‑1 (MQ=255)
                                                           tGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGa                                                                                  <  1:1262497/140‑1 (MQ=255)
                                                             gTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACTGGCTAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGAcg                                                                                >  1:68333/1‑140 (MQ=255)
                                                             gTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACTGGCTAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGAcg                                                                                >  1:68297/1‑140 (MQ=255)
                                                             gTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGAcg                                                                                <  2:949071/140‑1 (MQ=255)
                                                             gTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGAcg                                                                                <  1:1618379/140‑1 (MQ=255)
                                                              tAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGAcgc                                                                               <  1:1100638/140‑1 (MQ=255)
                                                                   aaCGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTta                                                                          <  2:711299/140‑1 (MQ=255)
                                                                   aaCGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTAAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTta                                                                          <  1:593586/140‑1 (MQ=255)
                                                                   aaCGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGATACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTta                                                                          <  2:182288/140‑1 (MQ=255)
                                                                           aGCGGCGCAAAAAGCGTTTCTTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGaaaaa                                                                  <  2:40808/140‑1 (MQ=255)
                                                                              ggcgCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACgg                                                               <  2:420895/140‑1 (MQ=255)
                                                                              ggcgCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACgg                                                               <  1:10133/140‑1 (MQ=255)
                                                                                 gcAAAAAGCGTTACGTTCTTCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCATTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGa                                                            <  1:853663/140‑1 (MQ=255)
                                                                                 caaaaaaGCTTTTCTTTCATCCGCTTTTTGCTACGAACGCCCAGAAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTACTACCATCTCCCCTTTGACGCGGTTATACAAAAACGGCGa                                                            <  1:853610/138‑1 (MQ=255)
                                                                                  cAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGGTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAATCGGCGAg                                                           >  1:1044565/1‑140 (MQ=255)
                                                                                  cAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAg                                                           >  1:1614813/1‑140 (MQ=255)
                                                                                  cAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAg                                                           >  1:1614915/1‑140 (MQ=255)
                                                                                  cAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAg                                                           >  1:548221/1‑140 (MQ=255)
                                                                                    aaaaGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTg                                                         <  2:792195/140‑1 (MQ=255)
                                                                                                       gCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCa                                      <  2:1147221/140‑1 (MQ=255)
                                                                                                        cTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCAc                                     <  1:1102101/140‑1 (MQ=255)
                                                                                                        cTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGAAATGGTCAATTTCGGCCTGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCAc                                     <  1:1129198/140‑1 (MQ=255)
                                                                                                         tGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACt                                    <  1:1069266/140‑1 (MQ=255)
                                                                                                          gTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTg                                   <  1:1563466/140‑1 (MQ=255)
                                                                                                              gCTACGATCGCCCAGTAACATCGACTGGCTAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTa                               <  2:68297/140‑1 (MQ=255)
                                                                                                              gCTACGATCGCCCAGTAACATCGACTGGCTAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTa                               <  2:68333/140‑1 (MQ=255)
                                                                                                              gCTACGATCGCCCAGTAACATCGACGGGCGTGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTa                               <  1:1445641/140‑1 (MQ=255)
                                                                                                              gCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTa                               <  1:906556/140‑1 (MQ=255)
                                                                                                              gCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTa                               <  2:1160059/140‑1 (MQ=255)
                                                                                                              gCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTa                               <  2:1357645/140‑1 (MQ=255)
                                                                                                                tACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCTGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTAcc                             <  2:1007696/140‑1 (MQ=255)
                                                                                                                tACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTAcc                             <  1:1109330/140‑1 (MQ=255)
                                                                                                                tACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTAcc                             <  1:687515/140‑1 (MQ=255)
                                                                                                                tACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAAGTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTAcc                             <  1:495663/140‑1 (MQ=255)
                                                                                                                     tCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACat                        >  1:778683/1‑140 (MQ=255)
                                                                                                                     tCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGATTCTTCAATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGAACCCATCGCACTGACTACCAACat                        >  2:1148969/1‑140 (MQ=255)
                                                                                                                     tCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGAGTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACat                        >  2:708221/1‑140 (MQ=255)
                                                                                                                         ccAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATAtgc                    <  2:450721/140‑1 (MQ=255)
                                                                                                                                 aTCGACGGGCGAGCAATGGTCAATTTCGGCCATTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGcc            <  1:66037/140‑1 (MQ=255)
                                                                                                                                 aTCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGcc            <  1:66365/140‑1 (MQ=255)
                                                                                                                                   cGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCa          >  2:1265884/1‑140 (MQ=255)
                                                                                                                                   cGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCa          >  2:597724/1‑140 (MQ=255)
                                                                                                                                     aCGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCGTTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGt        >  1:729812/1‑140 (MQ=255)
                                                                                                                                     aCGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGt        >  1:729838/1‑140 (MQ=255)
                                                                                                                                     aCGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGt        >  1:750649/1‑140 (MQ=255)
                                                                                                                                     aCGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGt        >  1:442595/1‑140 (MQ=255)
                                                                                                                                     aCGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGt        >  1:1424217/1‑140 (MQ=255)
                                                                                                                                     aCGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGt        >  1:1424076/1‑140 (MQ=255)
                                                                                                                                     aCGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGt        >  1:1423584/1‑140 (MQ=255)
                                                                                                                                     aCGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGt        >  1:1139505/1‑140 (MQ=255)
                                                                                                                                          cGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACCTATGCTGCGCGCCCAGTcgccg   <  2:973377/140‑1 (MQ=255)
                                                                                                                                          cGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGTcgccg   <  2:564589/140‑1 (MQ=255)
                                                                                                                                          cGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGTcgccg   <  2:564574/140‑1 (MQ=255)
                                                                                                                                           gAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGTcgccgc  >  2:666483/1‑140 (MQ=255)
                                                                                                                                           gAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGTcgccgc  <  1:1142410/140‑1 (MQ=255)
                                                                                                                                           gAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGTcgccgc  <  1:1138787/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ATCGACTCTGCCAGCATGACCCGCGCCACGTCTCTCGCATCAATGGATTTCCAGTTACCTGGTAACAAACGGAACAGCGGCGCAAAAAGCGTTTCGTTCATCCGCTGTTTGCTACGATCGCCCAGTAACATCGACGGGCGAGCAATGGTCAATTTCGGCCAGTTCTGGGCGATTAATGCTTCTTCCATCTCCCCTTTGACGCGGTTATAGAAAAACGGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGTCGCCGC  >  NC_000913/3298275‑3298553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: