Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,392,880	G→A	5.1%	R1127C (CGC→TGC)	yhdP ←	DUF3971‑AsmA2 domains protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,392,880	0	G	A	5.1%	276.1 / 3.8	78	R1127C (CGC→TGC)	yhdP	DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base G (49/25);  new base A (2/2);  total (51/27)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

ACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCGTAATCCACATTAA  >  NC_000913/3392741‑3393017                                                                                                                                            |                                                                                                                                          accaGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAgcg                                                                                                                                           <  1:1419803/140‑1 (MQ=255) accaGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAgcg                                                                                                                                           <  1:134395/140‑1 (MQ=255)  ccaGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGATGCTAATGGAGTCAAAATAGATCCCTTCGCCAAAAGTGTCTCTGAAATCTAAAGGCAGCTTTCGCATCAGGGCATCTACGCTCAATAAgcgc                                                                                                                                          <  1:784093/140‑1 (MQ=255)        tgtCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCCAAACGCAGCTGACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAAt                                                                                                                                    >  1:511870/1‑140 (MQ=255)        tgtCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAAt                                                                                                                                    >  1:1521128/1‑140 (MQ=255)          tcgtcgGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTg                                                                                                                                  >  1:295200/1‑140 (MQ=255)          tcgtcgGAGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGACTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATTAGCGCAGCACTTg                                                                                                                                  >  2:74841/1‑140 (MQ=255)           cgtcgGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGc                                                                                                                                 >  2:1354329/1‑140 (MQ=255)                   tgCATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATg                                                                                                                         >  1:1483043/1‑140 (MQ=255)                   tgCATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATg                                                                                                                         >  1:1482953/1‑140 (MQ=255)                     cATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTc                                                                                                                       <  2:259540/140‑1 (MQ=255)                     cATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTc                                                                                                                       <  2:1271657/140‑1 (MQ=255)                           gCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTAt                                                                                                                 >  1:1614098/1‑140 (MQ=255)                             cGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTg                                                                                                               >  2:234860/1‑140 (MQ=255)                               tCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGAt                                                                                                             >  1:1616956/1‑140 (MQ=255)                               tCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGAt                                                                                                             >  2:1582364/1‑140 (MQ=255)                               tCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGAt                                                                                                             >  2:1311376/1‑140 (MQ=255)                               tCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGAt                                                                                                             >  1:590948/1‑140 (MQ=255)                                    aaTCCACGCGGTGCTGCGAATGGAGTCAAAAGAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCgg                                                                                                        <  1:844747/140‑1 (MQ=255)                                         aCGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAAtt                                                                                                   >  2:837827/1‑140 (MQ=255)                                          cgcgGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAAttt                                                                                                  >  2:1577518/1‑140 (MQ=255)                                          cgcgGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAAttt                                                                                                  >  1:988465/1‑140 (MQ=255)                                          cgcgGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAAttt                                                                                                  >  1:504886/1‑140 (MQ=255)                                          cgcgGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAAttt                                                                                                  >  2:1048514/1‑140 (MQ=255)                                          cgcgGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGATCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAAttt                                                                                                  >  1:749685/1‑140 (MQ=255)                                          cgcgGTGCAGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAAttt                                                                                                  <  1:732066/140‑1 (MQ=255)                                          cgcgGTGCAGCGAATGGAGTCAAAATAGAACCCTACGCCAAAAGTGTCTCTGAACTCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAAttt                                                                                                  <  1:513247/140‑1 (MQ=255)                                                       aTGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAg                                                                                     <  2:1380801/140‑1 (MQ=255)                                                         ggAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGtt                                                                                   <  2:1360363/140‑1 (MQ=255)                                                         ggAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGtt                                                                                   >  1:976252/1‑140 (MQ=255)                                                         ggAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGtt                                                                                   >  1:1376096/1‑140 (MQ=255)                                                         ggAGTCAAAATAGAACCCTTCGACAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATATACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGtt                                                                                   <  1:1311376/140‑1 (MQ=255)                                                                     gAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTatgaatga                                                                       <  2:1194485/140‑1 (MQ=255)                                                                     gAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTatgaatga                                                                       >  1:588907/1‑140 (MQ=255)                                                                     gAACCCTTCGCAAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTatgaatga                                                                       <  2:333830/140‑1 (MQ=255)                                                                       aCCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAatgatg                                                                     <  2:425752/140‑1 (MQ=255)                                                                           ttCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCAt                                                                 >  1:1231758/1‑140 (MQ=255)                                                                           ttCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCAt                                                                 >  2:433324/1‑140 (MQ=255)                                                                           ttCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCAt                                                                 >  1:1111538/1‑140 (MQ=255)                                                                           ttCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCAt                                                                 >  1:1110015/1‑140 (MQ=255)                                                                           ttCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCAt                                                                 >  2:161269/1‑140 (MQ=255)                                                                           ttCGCCAAAAGTGTCTCTGAAATCAAAAAGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGAAATTGCCCGGCATGTACGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATTCCAt                                                                 >  2:978922/1‑140 (MQ=255)                                                                                cAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAAc                                                            <  1:1186267/140‑1 (MQ=255)                                                                                 aaaaGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACg                                                           <  2:1471587/140‑1 (MQ=255)                                                                                 aaaaGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACg                                                           <  2:1469789/140‑1 (MQ=255)                                                                                    aGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCg                                                        <  1:803987/140‑1 (MQ=255)                                                                                        tctcTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTc                                                    >  1:1330079/1‑140 (MQ=255)                                                                                        tctcTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTc                                                    >  1:1395165/1‑140 (MQ=255)                                                                                        tctcTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTc                                                    >  2:645559/1‑140 (MQ=255)                                                                                        tctcTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCAGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTc                                                    >  1:363055/1‑140 (MQ=255)                                                                                        tctcTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCACAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTc                                                    <  1:417787/140‑1 (MQ=255)                                                                                            tGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCt                                                <  2:1231758/140‑1 (MQ=255)                                                                                                      aCGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAgg                                      <  1:1204630/140‑1 (MQ=255)                                                                                                      aCGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAgg                                      <  1:275906/140‑1 (MQ=255)                                                                                                      aCGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAgg                                      <  1:274625/140‑1 (MQ=255)                                                                                                           gCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCt                                 <  1:1377554/140‑1 (MQ=255)                                                                                                                cGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTgcg                            <  2:501340/140‑1 (MQ=255)                                                                                                                 gCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTgcgc                           <  1:620542/140‑1 (MQ=255)                                                                                                                   aTCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCa                         <  1:1565940/140‑1 (MQ=255)                                                                                                                      aGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAgtg                      >  2:484098/1‑140 (MQ=255)                                                                                                                      aGGGCATCTACGCTCAATAAGCGCAGCAATTGACCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGAATGAATGATGCCATTCAACGTCGCTACATCTGGCTGCCAGGGTGCTTTGCGCCAgtt                      >  2:1486691/1‑139 (MQ=255)                                                                                                                        ggCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTa                    <  2:767223/140‑1 (MQ=255)                                                                                                                            tCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATc                >  1:1081515/1‑140 (MQ=255)                                                                                                                            tCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATc                >  1:512802/1‑140 (MQ=255)                                                                                                                            tCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATc                >  1:513031/1‑140 (MQ=255)                                                                                                                             cTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGTGCCAGTGTAAATCg               >  1:1177377/1‑140 (MQ=255)                                                                                                                             cTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCg               >  1:1617501/1‑140 (MQ=255)                                                                                                                             cTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCg               >  2:1377913/1‑140 (MQ=255)                                                                                                                             cTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCg               >  1:262962/1‑140 (MQ=255)                                                                                                                             cTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCg               >  1:1318687/1‑140 (MQ=255)                                                                                                                             cTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCg               >  1:918330/1‑140 (MQ=255)                                                                                                                             cTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCg               >  1:918308/1‑140 (MQ=255)                                                                                                                                     aaTAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCTTCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCGTAATCcac       >  2:1177571/1‑140 (MQ=255)                                                                                                                                     aaTAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCGTAATCcac       >  2:71437/1‑140 (MQ=255)                                                                                                                                     aaTAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCGTAATCcac       >  2:613373/1‑140 (MQ=255)                                                                                                                                     aaTAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCAGTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCGTAGTCcac       >  1:874532/1‑140 (MQ=255)                                                                                                                                     aaTAAGCACAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTa                    >  1:524967/1‑127 (MQ=255)                                                                                                                                     aaTAAGCACAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTa                    >  1:524456/1‑127 (MQ=255)                                                                                                                                     aaTAAGCACAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTa                    <  2:524967/127‑1 (MQ=255)                                                                                                                                     aaTAAGCACAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTa                    <  2:524456/127‑1 (MQ=255)                                                                                                                                       tAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCGTAATCCACAt     >  1:384525/1‑140 (MQ=255)                                                                                                                                          gcgcAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCGTAATCCACATTaa  >  1:1088461/1‑140 (MQ=255)                                                                                                                                          gcgcAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCGTAATCCACATTaa  >  2:1549125/1‑140 (MQ=255)                                                                                                                                          gcgcAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCGTAATCCACATTaa  >  1:1088186/1‑140 (MQ=255)                                                                                                                                          gcgcAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCAGTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCGTAATCCACAATaa  >  2:152715/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                          ACCAGCGTGTCGTCGGTGTGCATAACGCCGTCTTTAATCCACGCGGTGCTGCGAATGGAGTCAAAATAGAACCCTTCGCCAAAAGTGTCTCTGAAATCAAAACGCAGCTTACGCATCAGGGCATCTACGCTCAATAAGCGCAGCAATTGCCCGGCATGTCCGGTATTGATTTCGGTAATTTCGCCTTTACCCAGTTGAGTATGAATGATGCCATTCAACGTCGCTTCATCTGGCTGCCAGGGTGCTTTGCGCCAGTGTAAATCGTAATCCACATTAA  >  NC_000913/3392741‑3393017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑