Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,437,445	G→A	14.3%	A310T (GCC→ACC)	trkA →	NAD‑binding component of TrK potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,437,445	0	G	A	14.3%	199.5 / 21.9	70	A310T (GCC→ACC)	trkA	NAD‑binding component of TrK potassium transporter
Reads supporting (aligned to +/- strand):  ref base G (28/32);  new base A (3/7);  total (31/39)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGCAACT  >  NC_000913/3437307‑3437579                                                                                                                                           |                                                                                                                                       cagcagCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGc                                                                                                                                       <  1:68236/140‑1 (MQ=255) cagcagCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGc                                                                                                                                       >  1:404355/1‑140 (MQ=255) cagcagCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGc                                                                                                                                       <  2:466350/140‑1 (MQ=255)    cagcGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCaa                                                                                                                                    >  2:677977/1‑140 (MQ=255)    cagcGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCaa                                                                                                                                    >  2:651095/1‑140 (MQ=255)      gcgcgcTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAata                                                                                                                                  >  1:153112/1‑140 (MQ=255)      gcgcgcTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAata                                                                                                                                  >  1:153385/1‑140 (MQ=255)      gcgcgcTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAata                                                                                                                                  >  1:153425/1‑140 (MQ=255)       cgcgcTGCCGAACTGGCGGAAAAGTTACTTAATACGATCGTCTTTTTTGGTGATGCGTCGGTTCAAGTACTACTGGCCGAAGTACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACTTTGACGAGGCCagttt                                                                                                                                 >  2:1064444/1‑136 (MQ=255)       cgcgcTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAATTACATATCGATCAAGATGGTCTGGTTATTGCCGTCACCCACGATGCCGTGGCCTatat                                                                                                                                 >  2:42757/1‑140 (MQ=255)         cgcTGCCGAACTGGCGGAAAAGTTACAGAATGCGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGACGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCa                                                                                                                               >  1:1392711/1‑140 (MQ=255)         cgcTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCa                                                                                                                               >  1:1392768/1‑140 (MQ=255)               cGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCg                                                                                                                         <  1:752082/140‑1 (MQ=255)               cGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCg                                                                                                                         <  1:1472405/140‑1 (MQ=255)               cGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCg                                                                                                                         <  1:1472363/140‑1 (MQ=255)               cGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATATGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCg                                                                                                                         <  1:1463991/140‑1 (MQ=255)                      gCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATg                                                                                                                    >  2:1439239/1‑138 (MQ=255)                      gCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATg                                                                                                                    <  1:1439239/138‑1 (MQ=255)                        ggAAAAGTTTCAGAATACGCTCGTCTTATTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAAAGATGACGAGGCCAAACTCCTGTCCGCAATGCTTg                                                                                                                <  2:1327353/140‑1 (MQ=255)                          aaaaGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGcc                                                                                                              <  1:1492920/140‑1 (MQ=255)                          aaaaGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGcc                                                                                                              <  1:900988/140‑1 (MQ=255)                          aaaaGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGcc                                                                                                              <  1:1426147/140‑1 (MQ=255)                          aaaaGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGcc                                                                                                              <  1:900531/140‑1 (MQ=255)                          aaaaGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGcc                                                                                                              <  1:1425540/140‑1 (MQ=255)                                    gAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATgg                                                                                                    >  1:679358/1‑140 (MQ=255)                                         cGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCg                                                                                               >  2:224158/1‑140 (MQ=255)                                               tCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGaaaatg                                                                                         >  1:1021132/1‑138 (MQ=255)                                                   ttttGGTGCTGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGa                                                                                     <  2:1594401/140‑1 (MQ=255)                                                   ttttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGa                                                                                     <  2:1464405/140‑1 (MQ=255)                                                   ttttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGa                                                                                     <  2:1579532/140‑1 (MQ=255)                                                   ttttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGa                                                                                     <  2:1064024/140‑1 (MQ=255)                                                   ttttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGa                                                                                     <  2:1317189/140‑1 (MQ=255)                                                   ttttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGa                                                                                     <  2:298728/140‑1 (MQ=255)                                                   ttttGGAGATGCGTCGGAACAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGTAAAAGGTGa                                                                                     <  1:796951/140‑1 (MQ=255)                                                    tttGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGAt                                                                                    <  2:118988/140‑1 (MQ=255)                                                        gTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTa                                                                                >  2:368979/1‑140 (MQ=255)                                                         tGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTAt                                                                               <  1:1511697/140‑1 (MQ=255)                                                              cGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATc                                                                          <  1:1305662/140‑1 (MQ=255)                                                              cGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATc                                                                          <  2:1179221/140‑1 (MQ=255)                                                              cGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATc                                                                          <  1:1305643/140‑1 (MQ=255)                                                                        aGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTcgcg                                                                <  1:1531695/140‑1 (MQ=255)                                                                         gAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCgcgc                                                               <  1:818411/140‑1 (MQ=255)                                                                          aaCTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCt                                                              <  1:1264799/140‑1 (MQ=255)                                                                          aaCTACTGGCCGAAGAACATATCGATCAAGTTGATCTGATTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCt                                                              <  1:1266055/140‑1 (MQ=255)                                                                              actGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAtg                                                          >  2:1159828/1‑140 (MQ=255)                                                                              actGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAtg                                                          >  2:1044632/1‑140 (MQ=255)                                                                              actGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAtg                                                          <  2:447648/140‑1 (MQ=255)                                                                              actGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGACACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTAtg                                                          <  2:792656/140‑1 (MQ=255)                                                                                 ggCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATTTACGCCATTCTTGCCAAACGTATGGTTGCGAATATGGTGATTGTATTGATCCAGCGTCGCGCTTATGTgg                                                       >  2:1631051/1‑140 (MQ=255)                                                                                    cGAAGAACATCTCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAACAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATc                                                    <  1:1111209/140‑1 (MQ=255)                                                                                    cGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATc                                                    <  1:1389152/140‑1 (MQ=255)                                                                                    cGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATc                                                    <  2:404007/140‑1 (MQ=255)                                                                                            atatCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTGATGTGGATCTGGTTctg                                            >  1:198537/1‑138 (MQ=255)                                                                                                cGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTCTGGGTGCGAAAAAGGTCATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGa                                        <  2:234088/140‑1 (MQ=255)                                                                                                cGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAggggt                                        <  2:862478/140‑2 (MQ=255)                                                                                                  aTCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGc                                      >  2:718267/1‑140 (MQ=255)                                                                                                   tCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCg                                     >  1:614511/1‑140 (MQ=255)                                                                                                   tCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCg                                     >  1:1544573/1‑140 (MQ=255)                                                                                                   tCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTGATGTGGATCTGGTTCAGGGGAGCg                                     >  2:643726/1‑140 (MQ=255)                                                                                                    cAAGTTGATCTGTTTATTGCCGTCTCCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGt                                    <  2:1081089/140‑1 (MQ=255)                                                                                                        ttGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATc                                >  2:1509752/1‑140 (MQ=255)                                                                                                        ttGATCTGTTTATTGCCGTCACCAACGATGACGAGACCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATc                                >  2:1318804/1‑140 (MQ=255)                                                                                                               gTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTg                         >  2:1127963/1‑140 (MQ=255)                                                                                                                 ttATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCg                       >  1:587094/1‑140 (MQ=255)                                                                                                                          tCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTcacca              >  1:131336/1‑140 (MQ=255)                                                                                                                          tCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTcacca              >  1:1270645/1‑140 (MQ=255)                                                                                                                          tCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGAGATTGCGATTTcacca              >  1:131069/1‑140 (MQ=255)                                                                                                                                  gATGACGAGGCCAATATCATGTACGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGc      <  2:380189/140‑1 (MQ=255)                                                                                                                                     gACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGCAAc   <  2:1108904/140‑1 (MQ=255)                                                                                                                                      aCGAGGACAAAATCATGTCAGCCATGCTTGCCAAACGTATGTGTGCGAAAAAGGTGATGGTAATTATCCAGCGTCGCGCATAAGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGAATTCACCACAACAAGCAACt  >  1:968213/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                       CAGCAGCGCGCTGCCGAACTGGCGGAAAAGTTACAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTACTGGCCGAAGAACATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGTATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTTATCGATATTGCGATTTCACCACAACAAGCAACT  >  NC_000913/3437307‑3437579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑