Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,543,101 C→A 5.3% intergenic (+137/‑66) feoC → / → yhgA putative DNA‑binding transcriptional regulator/transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,543,1010CA 5.3% 301.4 / 2.3 83intergenic (+137/‑66)feoC/yhgAputative DNA‑binding transcriptional regulator/transposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base C (36/42);  new base A (2/3);  total (38/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTTACTCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACA  >  NC_000913/3542964‑3543233
                                                                                                                                         |                                                                                                                                    
aCCTTACTCCCTCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGc                                                                                                                                    <  2:1689486/140‑1 (MQ=255)
aCCTTACTCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGc                                                                                                                                    <  1:1573635/140‑1 (MQ=255)
  cTTACTCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTa                                                                                                                                  <  1:793817/140‑1 (MQ=255)
     aCTCCATCGCCTGTTTTGGAAAGCCCTGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATa                                                                                                                               >  2:8704/1‑140 (MQ=255)
     aCTCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATa                                                                                                                               >  2:1409264/1‑140 (MQ=255)
     aCTCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATa                                                                                                                               >  2:1408964/1‑140 (MQ=255)
     aCTCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATa                                                                                                                               >  2:992474/1‑140 (MQ=255)
     aCTCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATa                                                                                                                               >  2:1101320/1‑140 (MQ=255)
     aCTCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATa                                                                                                                               >  1:1333213/1‑140 (MQ=255)
     aCTCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATa                                                                                                                               >  2:408159/1‑140 (MQ=255)
     aCTCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATa                                                                                                                               >  2:897792/1‑140 (MQ=255)
     aCTCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATa                                                                                                                               >  2:513990/1‑140 (MQ=255)
     aCTCCATCGCCTGATTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCAGAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCAACTCGCAGGGATGAAAACTCGCTAATa                                                                                                                               >  2:1253560/1‑140 (MQ=255)
      cTCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATac                                                                                                                              >  1:394482/1‑140 (MQ=255)
       tCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTTCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATaca                                                                                                                             <  2:932757/140‑1 (MQ=255)
       tCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATaca                                                                                                                             <  1:638267/140‑1 (MQ=255)
       tCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATaca                                                                                                                             <  2:735855/140‑1 (MQ=255)
       tCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATaca                                                                                                                             <  1:781549/140‑1 (MQ=255)
       tCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATaca                                                                                                                             <  1:1044691/140‑1 (MQ=255)
       tCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATaca                                                                                                                             <  2:932534/140‑1 (MQ=255)
       tCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATaca                                                                                                                             <  1:1133355/140‑1 (MQ=255)
       tCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATaca                                                                                                                             <  1:1567388/140‑1 (MQ=255)
       tCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATaca                                                                                                                             <  1:1567292/140‑1 (MQ=255)
        ccATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATacac                                                                                                                            <  1:341977/140‑1 (MQ=255)
           tCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAgg                                                                                                                         >  1:442469/1‑140 (MQ=255)
           tCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAgg                                                                                                                         >  1:1095612/1‑140 (MQ=255)
           tCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAgg                                                                                                                         >  1:1095691/1‑140 (MQ=255)
               cTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGtgtg                                                                                                                     <  1:1672422/140‑1 (MQ=255)
                 gTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGa                                                                                                                   <  2:235337/140‑1 (MQ=255)
                    ttGGAAAGCCCGGTCTGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTg                                                                                                                <  1:596258/140‑1 (MQ=255)
                    ttGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTg                                                                                                                <  2:295385/140‑1 (MQ=255)
                    ttGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTg                                                                                                                <  1:804685/140‑1 (MQ=255)
                    ttGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTg                                                                                                                <  1:1525665/140‑1 (MQ=255)
                        aaaGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGgcgc                                                                                                            <  2:962578/140‑1 (MQ=255)
                            cccGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAATCTCGCTAATACACAGGTGTGGAGTGGCGCGTag                                                                                                        >  1:717096/1‑140 (MQ=255)
                            cccGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTag                                                                                                        >  1:848923/1‑140 (MQ=255)
                            cccGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTag                                                                                                        >  2:212204/1‑140 (MQ=255)
                                       tCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCATCTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCAt                                                                                             <  2:778280/140‑1 (MQ=255)
                                       tCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTAGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCAt                                                                                             >  1:1413649/1‑140 (MQ=255)
                                       tCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTAGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCAt                                                                                             >  1:1412672/1‑140 (MQ=255)
                                        cTGCATCCGGGCTTTTTTGCTTGCGGCTTTACATATAATTGCTTCTCTTGCAGCTCGTCGTAAGTTCCTTTGATAGCTTATCGCTGGGATGTAAACTCGCTAATCCACAGGTGTGGTGTGGCGAGTAGTGTCGCGGCAtt                                                                                            <  2:1665279/140‑1 (MQ=255)
                                         tGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTc                                                                                           >  1:537835/1‑140 (MQ=255)
                                          gCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCa                                                                                          >  1:54743/1‑140 (MQ=255)
                                          gCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCa                                                                                          >  1:504621/1‑140 (MQ=255)
                                              ccGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGAGTTAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAaaca                                                                                      <  1:446899/140‑1 (MQ=255)
                                               cGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAaacaa                                                                                     <  2:1516991/140‑1 (MQ=255)
                                               cGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAaacaa                                                                                     <  2:1516987/140‑1 (MQ=255)
                                               cGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAaacaa                                                                                     <  1:757470/140‑1 (MQ=255)
                                               cGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAaacaa                                                                                     <  1:211307/140‑1 (MQ=255)
                                               cGGGCTTTTTTGCGTGCGGCTTTCAATAAAAATGCAACTCTTGCAGCACGGCGTTAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAaacaa                                                                                     <  2:31157/140‑1 (MQ=255)
                                               cGGGCTTTTTTGCGTGAGGCTTTCCATAATAATGCATCTCTTGCAGCACGGCTTCAGTTCGTTTGAAAGCCTCTCGCAGGGATGTAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTTGTGTCGCGGCATTCaaaaaa                                                                                     <  2:100728/140‑1 (MQ=255)
                                                gggCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAaacaac                                                                                    >  1:343242/1‑140 (MQ=255)
                                                    ttttttGCGTGCGGCTTTCCATAAAAATGCAAATCTTGCATCACGGCGTTAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGt                                                                                <  2:844976/140‑1 (MQ=255)
                                                          gcgtgcgGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGa                                                                          <  2:998977/140‑1 (MQ=255)
                                                              gcgGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGc                                                                      >  1:1685830/1‑140 (MQ=255)
                                                                 gCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTAGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCAt                                                                   <  2:1413649/140‑1 (MQ=255)
                                                                 gCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTAGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCAt                                                                   <  2:1412672/140‑1 (MQ=255)
                                                                         tAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCaaaa                                                           <  1:774117/140‑1 (MQ=255)
                                                                            aaaTGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGc                                                        >  2:62282/1‑140 (MQ=255)
                                                                             aaTGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCa                                                       <  2:1342430/140‑1 (MQ=255)
                                                                             aaTGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCa                                                       <  1:1282263/140‑1 (MQ=255)
                                                                             aaTGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCa                                                       <  1:1482895/140‑1 (MQ=255)
                                                                             aaTGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCa                                                       <  1:785720/140‑1 (MQ=255)
                                                                               tGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCaga                                                     >  2:807505/1‑140 (MQ=255)
                                                                                 cAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGt                                                   >  1:1537493/1‑140 (MQ=255)
                                                                                       ttGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCAcc                                             <  1:1510282/140‑1 (MQ=255)
                                                                                            gcaCGGCGTAAGTTCCTTTGAAAGCAACTCGCAGGGATGTAAACTCGCTAATACACAGGTGAGGAGAGGCGCGTATAGTAGAGGCATTAAAACAACAGGTGACGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCcaca                                        >  2:990830/1‑140 (MQ=255)
                                                                                              aCGGCTTTAGTTGCTTTGAAAGCAACTCTGCGGGTTGAAATCTCGCTATAACTCAGGTGTGGTGTGGCGCGTATAGTCGCGGCATTCAAACTCCAGGTGAAGTATCGCCATGAGCAAAAAGCAGAGTTCCACCCCACACg                                      <  2:1585669/140‑1 (MQ=255)
                                                                                              aCGGCGTAAGTTCCTTTGAATGCATCTCGCTGGGATTATATCTAGCTAATACACAGGTTTGGTGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACg                                      <  2:542246/140‑1 (MQ=255)
                                                                                              aCGGCGTAAGTTCCTTTGAAATCTTCTCGCAGGGTTGAAATCTCGCTAATACACAGGTTTGGAGTGGCGCGTAGAGTCGCGGAATTCAAACAACAGGGTAAGGAACGCCATGAGCAAAAAGCTGAGTTCCACCCCACACg                                      <  2:1585572/140‑1 (MQ=255)
                                                                                                  cGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATaca                                                                                                                             <  2:858710/49‑1 (MQ=255)
                                                                                                  cGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATaca                                                                                                                             >  1:858710/1‑49 (MQ=255)
                                                                                                            tttGAAGGCATCTCGCAGGGATGAAAAATCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAAAAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCaaa                        >  2:938683/1‑140 (MQ=255)
                                                                                                            tttGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCaaa                        >  1:474490/1‑140 (MQ=255)
                                                                                                            tttGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCaaa                        >  1:1111578/1‑140 (MQ=255)
                                                                                                                 aaGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCtt                   >  1:1423822/1‑140 (MQ=255)
                                                                                                                 aaGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCtt                   >  2:1148936/1‑140 (MQ=255)
                                                                                                                  aGCATCTCGCAGGGATGAAAACTCGCTAATACTCAGGTGTGGAGTGGCGCGTTGTGTCGCGGCATTCAATCAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGAGCTGTTCGAACTCttg                  >  2:1679417/1‑139 (MQ=255)
                                                                                                                  aGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCttt                  >  1:45586/1‑140 (MQ=255)
                                                                                                                  aGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCttt                  >  2:448497/1‑140 (MQ=255)
                                                                                                                    cATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCttttt                >  1:649484/1‑140 (MQ=255)
                                                                                                                           cAGTGATGAAATCTCGCTAATACACAGGTGTGGAGTGGCGCGTATAGTCGCGGAATTGATCAAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGTTGCGCTGTTCAAACTCTTTTTAAGCCaa         <  1:1049671/140‑1 (MQ=255)
                                                                                                                                  gAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGaca  <  1:992474/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                    
ACCTTACTCCATCGCCTGTTTTGGAAAGCCCGGTATGCGTCTGCATCCGGGCTTTTTTGCGTGCGGCTTTCCATAAAAATGCAACTCTTGCAGCACGGCGTAAGTTCCTTTGAAAGCATCTCGCAGGGATGAAAACTCGCTAATACACAGGTGTGGAGTGGCGCGTAGAGTCGCGGCATTCAAACAACAGGTGAAGGAACGCCATGAGCAAAAAGCAGAGTTCCACCCCACACGATGCGCTGTTCAAACTCTTTTTACGCCAACCGGACA  >  NC_000913/3542964‑3543233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: