Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,892,094 C→A 7.6% F160L (TTC→TTA mdtL → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,892,0940CA 7.6% 268.9 / 7.2 79F160L (TTC→TTAmdtLmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (38/34);  new base A (3/3);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

TGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCC  >  NC_000913/3891961‑3892233
                                                                                                                                     |                                                                                                                                           
tGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCg                                                                                                                                       <  2:390675/140‑1 (MQ=255)
tGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCg                                                                                                                                       <  2:195539/140‑1 (MQ=255)
 gcgcGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGa                                                                                                                                      <  2:1310107/140‑1 (MQ=255)
           cTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAatgat                                                                                                                            <  2:70442/140‑1 (MQ=255)
              gatgatCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATggg                                                                                                                         <  1:708479/140‑1 (MQ=255)
              gatgatCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATggg                                                                                                                         <  2:101910/140‑1 (MQ=255)
              gatgatCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATggg                                                                                                                         <  1:707237/140‑1 (MQ=255)
                 gatCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCAt                                                                                                                      <  1:371812/140‑1 (MQ=255)
                 gatCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCAt                                                                                                                      <  1:371246/140‑1 (MQ=255)
                   tCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTTTTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATcg                                                                                                                    <  1:1603565/140‑1 (MQ=255)
                   tCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATcg                                                                                                                    <  2:1374021/140‑1 (MQ=255)
                   tCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATcg                                                                                                                    <  2:1294813/140‑1 (MQ=255)
                   tCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATcg                                                                                                                    <  1:684772/140‑1 (MQ=255)
                   tCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATcg                                                                                                                    <  1:1603131/140‑1 (MQ=255)
                   tCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATcg                                                                                                                    <  1:136377/140‑1 (MQ=255)
                   tCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATcg                                                                                                                    <  2:1055270/140‑1 (MQ=255)
                   tCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATcg                                                                                                                    <  1:1173214/140‑1 (MQ=255)
                   tCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGAGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATcg                                                                                                                    <  2:1475910/140‑1 (MQ=255)
                    cGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATcgc                                                                                                                   <  1:194556/140‑1 (MQ=255)
                    cGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTATGGGCGATGGCAATGATGGGCATcgc                                                                                                                   >  1:929851/1‑140 (MQ=255)
                    cGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTATGGGCGATGGCAATGATGGGCATcgc                                                                                                                   >  1:929755/1‑140 (MQ=255)
                    cGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTATGGGCGATGGCAATGATGGGCATcgc                                                                                                                   >  1:926413/1‑140 (MQ=255)
                             gCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGAt                                                                                                          >  2:353917/1‑140 (MQ=255)
                              cTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGAtg                                                                                                         <  1:1507555/140‑1 (MQ=255)
                               tAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGAtgt                                                                                                        <  2:1186595/140‑1 (MQ=255)
                               tAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGAtgt                                                                                                        <  2:1186659/140‑1 (MQ=255)
                               tAAAGTGCTGTCAATACACAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGAAGGGCATCGCGGTACTGAtgt                                                                                                        <  2:1428405/140‑1 (MQ=255)
                                      cTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCttt                                                                                                 >  1:416939/1‑140 (MQ=255)
                                      cTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCttt                                                                                                 >  1:827440/1‑140 (MQ=255)
                                      cTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCttt                                                                                                 >  1:553434/1‑140 (MQ=255)
                                      cTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCttt                                                                                                 >  1:820775/1‑140 (MQ=255)
                                      cTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCttt                                                                                                 >  1:819611/1‑140 (MQ=255)
                                       tGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTg                                                                                                >  2:933933/1‑140 (MQ=255)
                                       tGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTg                                                                                                >  2:933893/1‑140 (MQ=255)
                                              aCTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTAttt                                                                                         >  1:923649/1‑140 (MQ=255)
                                                   aCGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaaaa                                                                                    >  2:452776/1‑140 (MQ=255)
                                                    cGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAg                                                                                   >  2:926182/1‑140 (MQ=255)
                                                    cGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAg                                                                                   >  2:357434/1‑140 (MQ=255)
                                                       tATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGaaa                                                                                >  1:160709/1‑140 (MQ=255)
                                                        aTTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAAc                                                                               >  1:814613/1‑140 (MQ=255)
                                                              tGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGccc                                                                         >  1:679674/1‑140 (MQ=255)
                                                              tGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGccc                                                                         >  1:190396/1‑140 (MQ=255)
                                                               gCATCCTTCCGGTGTTAGCGCCAGGGCTCGCACAGCAGCGTATGCTGAAATTCCCGTGCCAGAGTCAGTACTCGGCGCTGGCACTCCTGGGCATCGCGGTACTGGGGGGGGCTGTGTTGATTTTAAAAGACCCGCGCCCa                                                                        >  2:361463/1‑140 (MQ=255)
                                                                catcatTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAg                                                                       >  2:313391/1‑140 (MQ=255)
                                                                 atcatTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGc                                                                      >  1:1642718/1‑140 (MQ=255)
                                                                 atcatTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGc                                                                      >  1:1516683/1‑140 (MQ=255)
                                                                 atcatTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGc                                                                      >  2:307608/1‑140 (MQ=255)
                                                                        cGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGccccc                                                               >  2:1186049/1‑140 (MQ=255)
                                                                         ggTGTTAGCGCCTGTGCTCGGATATCTGATTATGCTTTATTTTCGGTGGTTGAGTCTGTTGTGGGGGTTGGGTATTATGGTCATCGCGGTACTGATGTTGTCTTTGTTTTTTTTAAAAGAAACGCGCCCATCGGCCCCCg                                                              <  2:1300266/140‑1 (MQ=255)
                                                                         ggTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCg                                                              <  2:1267012/140‑1 (MQ=255)
                                                                         ggTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCg                                                              <  2:350583/140‑1 (MQ=255)
                                                                         ggTGTTAGCGCCAGTGCTCGGACATCTGATAATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCg                                                              <  2:1300920/140‑1 (MQ=255)
                                                                          gtgtTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGc                                                             >  1:566968/1‑140 (MQ=255)
                                                                               aGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCtt                                                        >  2:1054623/1‑140 (MQ=255)
                                                                               aGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCtt                                                        >  1:1564764/1‑140 (MQ=255)
                                                                               aGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCtt                                                        >  1:517958/1‑140 (MQ=255)
                                                                               aGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCtg                                                        >  2:1054598/1‑139 (MQ=255)
                                                                               aGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGGCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCtt                                                        >  1:518036/1‑140 (MQ=255)
                                                                               aGCGCAAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTAATGATGTTGTCTTTGATTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCta                                                        >  1:1526040/1‑139 (MQ=255)
                                                                                                 tCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGaaa                                      >  1:1523787/1‑140 (MQ=255)
                                                                                                 tCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGaaa                                      >  1:1135608/1‑140 (MQ=255)
                                                                                                 tCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGaaa                                      >  1:340719/1‑140 (MQ=255)
                                                                                                       tATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCg                                >  1:1560964/1‑140 (MQ=255)
                                                                                                        aTGCTTAAATTCCCGTGGCAGTGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGTTGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAATATAGCGa                               >  1:1167941/1‑140 (MQ=255)
                                                                                                          gCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGCTGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGt                             >  2:197640/1‑140 (MQ=255)
                                                                                                          gCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGt                             >  1:527065/1‑140 (MQ=255)
                                                                                                                   cccGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCCTCTCGGTACTGATGTTGGCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGAGAGTCgctgctga                    >  2:557847/1‑138 (MQ=255)
                                                                                                                   cccGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAATCCACGAGAAAATAGCGAGTCGCTGCTTa                    >  2:557770/1‑140 (MQ=255)
                                                                                                                         gCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGtt              <  2:416939/140‑1 (MQ=255)
                                                                                                                         gCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGtt              <  2:493034/140‑1 (MQ=255)
                                                                                                                         gCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGtt              <  1:262368/140‑1 (MQ=255)
                                                                                                                         gCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGtt              <  2:160709/140‑1 (MQ=255)
                                                                                                                         gCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGtt              <  2:1470012/140‑1 (MQ=255)
                                                                                                                              gTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTc         <  2:527065/140‑1 (MQ=255)
                                                                                                                                cTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCt       <  2:1429005/140‑1 (MQ=255)
                                                                                                                                cTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCt       <  2:832144/140‑1 (MQ=255)
                                                                                                                                cTGTTATGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCt       <  2:926413/140‑1 (MQ=255)
                                                                                                                                cTGTTATGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCt       <  2:929755/140‑1 (MQ=255)
                                                                                                                                cTGTTATGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCt       <  2:929851/140‑1 (MQ=255)
                                                                                                                                     cTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGcc  <  1:1606763/140‑1 (MQ=255)
                                                                                                                                     cTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGcc  <  1:275841/140‑1 (MQ=255)
                                                                                                                                     cTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGcc  <  1:307608/140‑1 (MQ=255)
                                                                                                                                     cTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGcc  <  2:1154584/140‑1 (MQ=255)
                                                                                                                                     cTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGcc  <  2:1154703/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                           
TGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCC  >  NC_000913/3891961‑3892233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: