Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,233,642	G→A	13.6%	intergenic (‑409/‑116)	lysC ← / → pgi	lysine‑sensitive aspartokinase 3/glucosephosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,233,642	0	G	A	13.6%	248.2 / 27.8	89	intergenic (‑409/‑116)	lysC/pgi	lysine‑sensitive aspartokinase 3/glucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (48/28);  new base A (6/6);  total (55/34)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

CGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACG  >  NC_000913/4233504‑4233778                                                                                                                                           |                                                                                                                                         cgcgCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTgc                                                                                                                                         >  2:1224719/1‑140 (MQ=255) cgcgCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTgc                                                                                                                                         >  2:660254/1‑140 (MQ=255) cgcgCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTgc                                                                                                                                         >  1:1348660/1‑140 (MQ=255) cgcgCCTCTTATGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTgc                                                                                                                                         >  2:1384126/1‑140 (MQ=255)  gcgcCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTATTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTgcg                                                                                                                                        >  1:1536708/1‑140 (MQ=255)     ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     <  1:918020/140‑1 (MQ=255)     ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     <  2:421345/140‑1 (MQ=255)     ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     <  2:316906/140‑1 (MQ=255)     ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     <  1:1580653/140‑1 (MQ=255)     ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     <  2:1338733/140‑1 (MQ=255)       tcttctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAg                                                                                                                                   <  2:1593767/140‑1 (MQ=255)        cttctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAgg                                                                                                                                  >  2:1529412/1‑140 (MQ=255)          tctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGt                                                                                                                                <  1:1144006/140‑1 (MQ=255)          tctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGt                                                                                                                                <  2:1035047/140‑1 (MQ=255)          tctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGt                                                                                                                                <  1:1143727/140‑1 (MQ=255)           ctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGtt                                                                                                                               >  1:1276816/1‑140 (MQ=255)           ctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGtt                                                                                                                               >  1:1276428/1‑140 (MQ=255)           ctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGtt                                                                                                                               >  1:1276397/1‑140 (MQ=255)             ggCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTa                                                                                                                             <  1:111376/140‑1 (MQ=255)              gCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTac                                                                                                                            >  1:31509/1‑140 (MQ=255)              gCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTGTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTac                                                                                                                            >  1:1272131/1‑140 (MQ=255)               ccTGCGCTAGCGCAGGTAGTACAATTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTTATAAATGCTTCACTGCGCTAAGGGTTTaca                                                                                                                           <  1:585594/140‑1 (MQ=255)                 tGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCTTGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACt                                                                                                                         <  1:659122/140‑1 (MQ=255)                 tGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACt                                                                                                                         <  2:1585433/140‑1 (MQ=255)                 tGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACt                                                                                                                         <  1:1017273/140‑1 (MQ=255)                 tGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACt                                                                                                                         <  1:1612474/140‑1 (MQ=255)                 tGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACt                                                                                                                         <  1:832386/140‑1 (MQ=255)                   cgcTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATTCTTCACTGCGCTAAGGGTTTACACTCa                                                                                                                       >  2:728064/1‑140 (MQ=255)                   cgcTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCa                                                                                                                       >  2:345730/1‑140 (MQ=255)                   cgcTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCa                                                                                                                       >  2:751576/1‑140 (MQ=255)                       aGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACAt                                                                                                                   <  2:1330291/140‑1 (MQ=255)                             ggTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTTGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACTCAACATTACGCt                                                                                                             >  1:82381/1‑140 (MQ=255)                             ggTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCt                                                                                                             >  1:868409/1‑140 (MQ=255)                             ggTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCt                                                                                                             >  2:802417/1‑140 (MQ=255)                             ggTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCt                                                                                                             >  1:348885/1‑140 (MQ=255)                             ggTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCt                                                                                                             >  2:1280465/1‑140 (MQ=255)                               tagtaCATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTaa                                                                                                           <  1:1683222/140‑1 (MQ=255)                                   aCATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGAAGTTCAGCTAATAAATGCTTCACTGAGCTAAGGGTTTACACTCAACAATACGCTAACGGc                                                                                                       >  2:1678468/1‑140 (MQ=255)                                    cATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     >  1:772981/1‑109 (MQ=255)                                    cATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     <  2:772981/109‑1 (MQ=255)                                        tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTaa                                                                                                  >  1:579433/1‑140 (MQ=255)                                        tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTaa                                                                                                  >  1:1189774/1‑140 (MQ=255)                                        tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTaa                                                                                                  >  1:1189739/1‑140 (MQ=255)                                        tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTaa                                                                                                  >  1:1418258/1‑140 (MQ=255)                                        tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTaa                                                                                                  >  2:1078427/1‑140 (MQ=255)                                        tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTaa                                                                                                  >  1:225081/1‑140 (MQ=255)                                            aataaaGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAAcc                                                                                              >  2:1140197/1‑140 (MQ=255)                                               aaaGGGTGAGCGGGGCGGTTGTCAACTATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAATCCATc                                                                                           <  2:877851/140‑1 (MQ=255)                                               aaaGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCTGATTTTTCATCCACTCCTTGCGGTCAGTAGTTCAGCTAATAAATGCTTCTCTACGCTAAGTGTTTACACTCAACTTTACGCTTACGGCACTACAACCATc                                                                                           <  2:244900/140‑1 (MQ=255)                                                 cgggTGAGCCGGGCGGATGTCAACGATTGGGTCATGCGGAATTTTCATCCACTACTGGCGGTCACTATTTCAGCTAATAAATGCTTCACAGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAAACATcac                                                                                         >  2:566307/2‑140 (MQ=255)                                                 aGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATcac                                                                                         >  1:1131175/1‑140 (MQ=255)                                                 aGGGTGAGCGGGGAGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGGTTACACTCAACAGTACGCTAACGGCACTAAAAACATcac                                                                                         >  2:566312/1‑140 (MQ=255)                                                   ggTGAGCGGGGCGGTTGTCAACGTTGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTATCGGCACTAAATCCATCACAt                                                                                       <  1:697437/140‑1 (MQ=255)                                                       aGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACAttttt                                                                                   >  1:787493/1‑140 (MQ=255)                                                       aGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACAttttt                                                                                   >  1:1662549/1‑140 (MQ=255)                                                       aGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACAttttt                                                                                   >  1:1633576/1‑140 (MQ=255)                                                             gCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGa                                                                             >  1:1573812/1‑140 (MQ=255)                                                              cGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGAc                                                                            <  1:451342/140‑1 (MQ=255)                                                                      aaCGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGTCGCTa                                                                    >  1:786611/1‑140 (MQ=255)                                                                        cGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACa                                                                  <  2:1470577/140‑1 (MQ=255)                                                                        cGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACa                                                                  <  2:1470477/140‑1 (MQ=255)                                                                           tGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGTGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACAATTTTCTGTGACTGGCGCTACAATc                                                               >  2:1531818/1‑140 (MQ=255)                                                                                   tGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAg                                                       >  1:762645/1‑140 (MQ=255)                                                                                   tGCGGATTTTTCATCCACTCCTGGCGGTAAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACAATACGCTAACGGCACTAAAACCATCACATTTATCTGTGACTGGAGCTACAATCTTCCAAAg                                                       >  2:413823/1‑140 (MQ=255)                                                                                          ttttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAAt                                                >  1:749703/1‑140 (MQ=255)                                                                                          ttttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAAt                                                >  1:519675/1‑140 (MQ=255)                                                                                              cATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACA‑TTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCa                                           >  2:762032/1‑140 (MQ=255)                                                                                                   aCTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAAt                                       <  1:1118951/140‑1 (MQ=255)                                                                                                   aCTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAAt                                       <  1:1604550/140‑1 (MQ=255)                                                                                                    cTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATc                                      >  1:1100956/1‑140 (MQ=255)                                                                                                      ccTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCag                                    <  1:370319/140‑1 (MQ=255)                                                                                                               cAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCATAGTCACAATTCTCAAAATCAGAAGAGTAtt                           >  1:1362760/1‑140 (MQ=255)                                                                                                               cAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTAtt                           >  2:809961/1‑140 (MQ=255)                                                                                                               cAGTAGTTCAGCAAATAAATCCTTCACTTCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACAAAAACCATCACAATTTTCAGTCACTGGCGCTACAAACAACCAAAGACAAAAATCTCAAAATCAGAAtattatg                           >  2:598238/1‑139 (MQ=255)                                                                                                                            aataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAAc              >  2:669849/1‑140 (MQ=255)                                                                                                                            aataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAAc              >  2:1098973/1‑140 (MQ=255)                                                                                                                            aataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAAc              >  2:1098977/1‑140 (MQ=255)                                                                                                                              taaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACAt            <  2:1536708/140‑1 (MQ=255)                                                                                                                              taaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACAt            <  1:1232727/140‑1 (MQ=255)                                                                                                                              taaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACAt            <  2:1647059/140‑1 (MQ=255)                                                                                                                                   gCTTCACTACGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATc       <  2:1027238/140‑1 (MQ=255)                                                                                                                                    cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATcc      >  2:346173/1‑140 (MQ=255)                                                                                                                                    cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATcc      >  1:411209/1‑140 (MQ=255)                                                                                                                                    cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATcc      >  2:346484/1‑140 (MQ=255)                                                                                                                                     ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCa     <  2:1661615/140‑1 (MQ=255)                                                                                                                                       cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:199520/1‑140 (MQ=255)                                                                                                                                       cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:140328/1‑140 (MQ=255)                                                                                                                                       cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:1639646/1‑140 (MQ=255)                                                                                                                                       cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:1639656/1‑140 (MQ=255)                                                                                                                                       cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:479469/1‑140 (MQ=255)                                                                                                                                       cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:1126070/1‑140 (MQ=255)                                                                                                                                       cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:199506/1‑140 (MQ=255)                                                                                                                                       cACTGCGCTAAGGGTTTACACTCAACATAACGCTAACGGCACTAAAACCATCACATTATTCTGTGACTGGCGATACAATCTTCCAAAGTCACAATTCTCAAAAACAGAAGAGTATTGCTAATGAAAAACAACAATCCAAc   >  1:1656942/1‑140 (MQ=255)                                                                                                                                        aCTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCAACACAATATACTGTGACTGGCGCTACAAACTACCAAACTCACAATTCTCAAAATCAGAACAGTATTGCTAATGAAAAACAACAATCCAACg  <  2:448547/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         CGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACG  >  NC_000913/4233504‑4233778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑