Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,244,074 C→A 7.5% L334F (TTG→TTT malF ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,244,0740CA 7.5% 232.1 / 5.1 67L334F (TTG→TTTmalFmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (29/33);  new base A (3/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

CAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGC  >  NC_000913/4243939‑4244212
                                                                                                                                       |                                                                                                                                          
cagccagGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGa                                                                                                                                        <  1:1398417/140‑1 (MQ=255)
  gccagGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGAtt                                                                                                                                      >  2:885690/1‑140 (MQ=255)
  gccagGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCATTGAAAATCAAGAtt                                                                                                                                      >  2:886059/1‑140 (MQ=255)
     agGTATTGACGATAATTAGCTTCGTGCGGTCGGTGGTCGGATCGCTGAACCAGGCTGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAAAAACCCTTTGAAAATCAAGATTGaa                                                                                                                                   <  1:1063456/140‑1 (MQ=255)
     agGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGaa                                                                                                                                   <  1:158868/140‑1 (MQ=255)
       gTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAAt                                                                                                                                 >  2:578245/1‑140 (MQ=255)
        tATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAAtt                                                                                                                                >  1:1013731/1‑139 (MQ=255)
        tATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATg                                                                                                                                >  1:1012081/1‑140 (MQ=255)
        gaTTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATg                                                                                                                                <  1:1689331/139‑1 (MQ=255)
             aCGAAAATTAGCATCGTGCGGGCGGTTGTATTATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTATGAAAATCAAGAATTAAAAGAActa                                                                                                                           >  1:336674/1‑138 (MQ=255)
                 tAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGc                                                                                                                       >  2:1317069/1‑140 (MQ=255)
                 tAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGc                                                                                                                       >  2:1105649/1‑140 (MQ=255)
                 tAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGc                                                                                                                       >  2:1102714/1‑140 (MQ=255)
                  aaTTAGCATCGTGCGGGCGGTGGTCGGATCGCTGACCCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCCCCGCAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCa                                                                                                                      <  2:731564/140‑1 (MQ=255)
                  aaTTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAACTCAAGATTGACATGAACGATGGCa                                                                                                                      <  2:1204145/140‑1 (MQ=255)
                       gCATCGTGCGGGCGGTGGTCGGATCTCTGACCCCGGCGGGCTTCACGCCAAACCGCGAGCTCAACATCATGTTGCTTTCACCGAAGCTCTGGTTATACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACcgcg                                                                                                                 <  2:1380144/140‑1 (MQ=255)
                        cATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGt                                                                                                                <  2:789640/140‑1 (MQ=255)
                             tGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGc                                                                                                           >  2:238036/1‑140 (MQ=255)
                             tGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGc                                                                                                           >  2:238023/1‑140 (MQ=255)
                             tGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGc                                                                                                           >  1:807139/1‑140 (MQ=255)
                             tGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGc                                                                                                           >  2:1176972/1‑140 (MQ=255)
                             tGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGc                                                                                                           >  1:1238394/1‑140 (MQ=255)
                             tGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATAAAGATTGAAATGAACGATGGCACCGCGTAGGGc                                                                                                           >  1:1420975/1‑140 (MQ=255)
                                     gtggtCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCCCCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATcag                                                                                                   <  2:1379743/140‑1 (MQ=255)
                                     gtggtCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATcag                                                                                                   <  2:1380010/140‑1 (MQ=255)
                                         tCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAgg                                                                                               <  1:1185465/140‑1 (MQ=255)
                                         tCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAgg                                                                                               <  2:540709/140‑1 (MQ=255)
                                         tCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAgg                                                                                               <  1:442751/140‑1 (MQ=255)
                                         tCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAgg                                                                                               <  2:1406338/140‑1 (MQ=255)
                                                   gtaacAGGCGGGCTTCACGCCAAACACCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGATATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGa                                                                                     <  2:233874/136‑1 (MQ=255)
                                                   gAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCACAATCATCTTGATTTCACCTAAGCTCTGGTTTAACAACCCTTTGAAAATCAACATTGAAATGAACGATGGCACCGCGTAGGGCATAATCAGCAGGACGCGATAGa                                                                                     <  2:233868/140‑1 (MQ=255)
                                                       cAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGAACGc                                                                                 >  1:15595/1‑140 (MQ=255)
                                                              gCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCcg                                                                          >  1:1018291/1‑140 (MQ=255)
                                                              gCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCcg                                                                          >  1:205665/1‑140 (MQ=255)
                                                                ttCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCcgcg                                                                        <  1:700139/140‑1 (MQ=255)
                                                                ttCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCcgcg                                                                        <  1:808896/140‑1 (MQ=255)
                                                                  cACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCa                                                                      <  2:1012081/140‑1 (MQ=255)
                                                                  cACGCCAAACAGCGCGCACAACAACATGTTGATTTCACCGAAGCTCTGGATAAACAACCCATTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCa                                                                      <  2:1013731/140‑1 (MQ=255)
                                                                   aCGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCaa                                                                     >  1:1492520/1‑140 (MQ=255)
                                                                       cAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCt                                                                 <  2:362357/140‑1 (MQ=255)
                                                                               gcgcTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTg                                                         <  2:362705/140‑1 (MQ=255)
                                                                                 gcTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCTCTGCa                                                       <  1:591108/140‑1 (MQ=255)
                                                                                      aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGa                                                  <  1:1264054/140‑1 (MQ=255)
                                                                                      aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGa                                                  <  1:238023/140‑1 (MQ=255)
                                                                                      aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGa                                                  <  2:906246/140‑1 (MQ=255)
                                                                                      aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGa                                                  <  1:238036/140‑1 (MQ=255)
                                                                                      aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGa                                                  <  1:677623/140‑1 (MQ=255)
                                                                                           atGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGc                                             <  1:1149511/140‑1 (MQ=255)
                                                                                             gTTGATATCAACGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCa                                           >  2:454005/1‑140 (MQ=255)
                                                                                               tGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGa                                         <  2:1010270/140‑1 (MQ=255)
                                                                                               tGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGa                                         >  1:369979/1‑140 (MQ=255)
                                                                                               tGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGa                                         <  2:663035/140‑1 (MQ=255)
                                                                                                 aTTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAAc                                       >  1:390988/1‑140 (MQ=255)
                                                                                                 aTTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATAAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTcc                                                              >  1:176117/1‑117 (MQ=255)
                                                                                                 aTTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATAAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTcc                                                              >  1:176144/1‑117 (MQ=255)
                                                                                                 aTTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATAAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTcc                                                              <  2:176117/117‑1 (MQ=255)
                                                                                                 aTTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATAAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTcc                                                              <  2:176144/117‑1 (MQ=255)
                                                                                                   ttCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCa                                     >  2:1265638/1‑140 (MQ=255)
                                                                                                           aGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAGCGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGAcc                             >  1:1686353/1‑140 (MQ=255)
                                                                                                           aGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGAcc                             >  1:233093/1‑140 (MQ=255)
                                                                                                             ctctGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGc                           <  1:992135/140‑1 (MQ=255)
                                                                                                               ctGGTTAAACAACCCTTTGAACATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCa                         <  1:645533/140‑1 (MQ=255)
                                                                                                                       acaacCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTaaa                 >  1:389116/1‑140 (MQ=255)
                                                                                                                       acaacCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTaaa                 >  1:121225/1‑140 (MQ=255)
                                                                                                                            ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGAc            >  1:1131647/1‑140 (MQ=255)
                                                                                                                                tGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTg        >  1:781945/1‑140 (MQ=255)
                                                                                                                                      tCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGc  >  2:337076/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                          
CAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGC  >  NC_000913/4243939‑4244212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: