Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,348,756 C→A 5.5% intergenic (‑12/+559) dcuB ← / ← dcuR C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor/response regulator in two‑component regulatory system with DcuS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,348,7560CA 5.5% 258.2 / 2.5 72intergenic (‑12/+559)dcuB/dcuRC4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor/response regulator in two‑component regulatory system with DcuS
Reads supporting (aligned to +/- strand):  ref base C (28/40);  new base A (2/2);  total (30/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

TGAAGGTGGAAGACGAAGACCAGAATGACCAGACCGATACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAGTGAATATTGGCTGATT  >  NC_000913/4348617‑4348895
                                                                                                                                           |                                                                                                                                           
tGAAGGTGGAAGACGAAGACCAGAATGACCAGACCGATACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTc                                                                                                                                             >  2:388294/1‑140 (MQ=255)
            acgaagacCAGAATGACCAGACCGATACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTa                                                                                                                                 >  1:1204885/1‑140 (MQ=255)
                        aTGACCAGACCGATACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTg                                                                                                                     <  2:472056/140‑1 (MQ=255)
                        aTGACCAGACCGATACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTg                                                                                                                     <  2:474237/140‑1 (MQ=255)
                        aTGACCAGACCGATACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTg                                                                                                                     <  2:1177162/140‑1 (MQ=255)
                        aTGACCAGACCGATACAGCCTAATAACCCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGAAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTg                                                                                                                     <  1:558625/140‑1 (MQ=255)
                          gACCAGACCGATACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTTAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCt                                                                                                                   <  1:1860/140‑1 (MQ=255)
                          gACCAGACCGATACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCt                                                                                                                   <  1:363365/140‑1 (MQ=255)
                                  cGATACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGAt                                                                                                           <  1:11612/140‑1 (MQ=255)
                                  cGATACCGCCTAATAAACCCAGCGCGATACCACCCTTACTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGAGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGAt                                                                                                           <  2:379823/140‑1 (MQ=255)
                                    aTACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTCAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATca                                                                                                         >  2:1056638/1‑140 (MQ=255)
                                    aTACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATca                                                                                                         <  1:974086/140‑1 (MQ=255)
                                    aTACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATca                                                                                                         <  1:697695/140‑1 (MQ=255)
                                    aTACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATca                                                                                                         <  1:588423/140‑1 (MQ=255)
                                         gCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACACTCGCGTTAATCCTATTTAAATTTTTGCTGAATAGATCACAGTc                                                                                                    <  2:222294/140‑1 (MQ=255)
                                          ccTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCa                                                                                                   >  2:1245058/1‑140 (MQ=255)
                                              ataaACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGtt                                                                                               <  1:1569582/140‑1 (MQ=255)
                                              ataaACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGtt                                                                                               <  2:915339/140‑1 (MQ=255)
                                              ataaACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGtt                                                                                               <  2:14513/140‑1 (MQ=255)
                                               taaACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTc                                                                                              >  1:345453/1‑140 (MQ=255)
                                                aaaCCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCt                                                                                             <  2:147199/140‑1 (MQ=255)
                                                  aCCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGt                                                                                           <  2:1127529/140‑1 (MQ=255)
                                                  aCCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGt                                                                                           <  2:1055711/140‑1 (MQ=255)
                                                     cAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTAGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGtttt                                                                                        >  2:1052315/1‑140 (MQ=255)
                                                     cAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTAGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGtttt                                                                                        >  2:1051751/1‑140 (MQ=255)
                                                      aGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTg                                                                                       >  2:39284/1‑140 (MQ=255)
                                                      aGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTg                                                                                       >  2:39379/1‑140 (MQ=255)
                                                        cgcgATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTa                                                                                     >  1:1231384/1‑140 (MQ=255)
                                                           gATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGa                                                                                  <  1:1266321/140‑1 (MQ=255)
                                                                         tGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGc                                                                    <  2:211736/140‑1 (MQ=255)
                                                                         tGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGc                                                                    <  2:804998/140‑1 (MQ=255)
                                                                         tGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGc                                                                    <  2:608030/140‑1 (MQ=255)
                                                                           gCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCt                                                                  <  1:867425/140‑1 (MQ=255)
                                                                                            tatCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTg                                                 >  1:1550390/1‑140 (MQ=255)
                                                                                             atCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTgg                                                <  1:198796/140‑1 (MQ=255)
                                                                                                   attatGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTa                                          >  2:43901/1‑140 (MQ=255)
                                                                                                   attatGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGACACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTa                                          >  2:44079/1‑140 (MQ=255)
                                                                                                     tatGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTAcc                                        >  1:663587/1‑140 (MQ=255)
                                                                                                       tGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCgg                                      >  1:126971/1‑140 (MQ=255)
                                                                                                       tGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCgg                                      >  2:1358806/1‑140 (MQ=255)
                                                                                                       tGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCgg                                      >  2:21313/1‑140 (MQ=255)
                                                                                                         aTAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCt                                    <  1:373891/140‑1 (MQ=255)
                                                                                                            aGTTGGATAGTAAATCACATGTGTGAACCCTCGCGATAATCCTATGTAAATTTGTGCTGACTAGTTCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACGAAATCTCCGTTTGGTCGTTACCGGCTTTa                                 <  2:1207485/140‑1 (MQ=255)
                                                                                                            aGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTa                                 <  1:388294/140‑1 (MQ=255)
                                                                                                            aGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTa                                 >  2:1079620/1‑140 (MQ=255)
                                                                                                            aGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTa                                 >  1:1091205/1‑140 (MQ=255)
                                                                                                            aGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTa                                 >  1:1478744/1‑140 (MQ=255)
                                                                                                            aGTTGGATAGTAAATAACAGGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTa                                 >  1:1476449/1‑140 (MQ=255)
                                                                                                              ttGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGc                               <  2:305028/140‑1 (MQ=255)
                                                                                                              ttGGATAGTAAATAACATGTGTGAACCCTAGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGc                               <  1:1052315/140‑1 (MQ=255)
                                                                                                              ttGGATAGTAAATAACATGTGTGAACCCTAGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGc                               <  1:1051751/140‑1 (MQ=255)
                                                                                                                ggATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCaa                             <  1:1120696/140‑1 (MQ=255)
                                                                                                                      tAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCt                       >  2:965433/1‑140 (MQ=255)
                                                                                                                      tAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCt                       <  2:1622745/140‑1 (MQ=255)
                                                                                                                      tAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCt                       <  2:1371143/140‑1 (MQ=255)
                                                                                                                      tAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCt                       <  2:1371126/140‑1 (MQ=255)
                                                                                                                      tAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCt                       <  2:302790/140‑1 (MQ=255)
                                                                                                                      tAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCt                       >  2:965427/1‑140 (MQ=255)
                                                                                                                      tAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCt                       <  1:1298577/140‑1 (MQ=255)
                                                                                                                      tAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCt                       <  1:1358806/140‑1 (MQ=255)
                                                                                                                       aaaTAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTc                      <  1:596711/140‑1 (MQ=255)
                                                                                                                        aaTAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTca                     >  1:291174/1‑140 (MQ=255)
                                                                                                                           aaCATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAg                  >  1:1573293/1‑140 (MQ=255)
                                                                                                                           aaCATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAg                  >  1:1193610/1‑140 (MQ=255)
                                                                                                                           aaCATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAg                  <  2:357867/140‑1 (MQ=255)
                                                                                                                           aaCATGTGTGAACCCTCGCGAGAAGCGTATTTAAATTTTTGCAGTATAGAGCGCAGTCACGTTCTGTTTAGGATGAAGTGGTTTCTGAGCCTGTCTAAATATGCGTTGGGGCGTTAACGGGTTTAGGAATTACCtaagag                  >  2:387715/1‑135 (MQ=255)
                                                                                                                                gtgtgAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAGTGAat             >  1:1174411/1‑140 (MQ=255)
                                                                                                                                gtgtgAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAGTGAat             >  1:1605426/1‑140 (MQ=255)
                                                                                                                                gtgtgAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAGTGAat             >  2:309667/1‑140 (MQ=255)
                                                                                                                                gtgtgAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAGTGAat             >  2:1510024/1‑140 (MQ=255)
                                                                                                                                     aaCCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAGTGAATATTgg        <  1:1553965/140‑1 (MQ=255)
                                                                                                                                     aaCCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAGTGAATATTgg        <  2:1424699/140‑1 (MQ=255)
                                                                                                                                     aaCCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAGTGAATATTgg        <  2:661523/140‑1 (MQ=255)
                                                                                                                                           cgcgATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAGTGAATATTGGCTGAtt  >  1:1248958/1‑140 (MQ=255)
                                                                                                                                           cgcgATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAGTGAATATTGGCTGAtt  >  1:1246349/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TGAAGGTGGAAGACGAAGACCAGAATGACCAGACCGATACCGCCTAATAAACCCAGCGCGATACCACCCTTTCTGGCACCATAAAACAGACATATCAGTATTATGATAAGTTGGATAGTAAATAACATGTGTGAACCCTCGCGATAATCCTATTTAAATTTTTGCTGAATAGATCACAGTCACGTTCTGTTTTGTATGAACTGTTTTCAGAGCCTGACTAAATATCCGTTTGGTCGTTACCGGCTTTAGCAAATACCTCACAGTGAATATTGGCTGATT  >  NC_000913/4348617‑4348895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: