BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,504,861	0	T	A	21.9%	519.6 / 11.3	267	intergenic (+78/+74)	frlR/yhfS	putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand): ref base T (31/158); new base A (50/3); total (106/161)
Fisher's exact test for biased strand distribution p-value = 1.47e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 303 total aligned reads displayed.

GTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATT  >  NC_000913/3504721‑3504994

gTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCT‑GCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  2:20591/140‑1 (MQ=255)

 tctcCGACCTCGACTGCCGCGCCAATCGCAACACCATAACTCTTGATCATAAAAGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATATTACCTCCCGTACGTTCCGCt                                                                                                                                        <  2:1150918/140‑1 (MQ=37)

 tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  2:1550780/140‑1 (MQ=255)

 tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  1:836586/140‑1 (MQ=255)

 tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  1:961140/140‑1 (MQ=255)

 tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  1:478438/140‑1 (MQ=255)

 tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  2:1369366/140‑1 (MQ=255)

 tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  1:169942/140‑1 (MQ=255)

 tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  2:162048/140‑1 (MQ=255)

 tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  1:1533726/140‑1 (MQ=255)

 tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  1:1422607/140‑1 (MQ=255)

 tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  2:818405/140‑1 (MQ=255)

 tctcCGAACTCTTCTGCAGCGACTATCGCATCACCTTAACTTTTGATAATAATAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  2:1798797/140‑1 (MQ=39)

 tctcCGAACTCTTCAGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  2:1477359/140‑1 (MQ=255)

     cGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACAcc                                                                                                                                    >  2:1406732/1‑140 (MQ=255)

      gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAccc                                                                                                                                   <  2:1127738/140‑1 (MQ=255)

       aaCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCt                                                                                                                                  <  2:642549/140‑1 (MQ=255)

        aCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTa                                                                                                                                 <  2:1719090/140‑1 (MQ=255)

           cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                              <  2:512456/140‑1 (MQ=255)

            ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGTTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCAACCAAAccc                                                                                                                             >  1:1748960/1‑140 (MQ=255)

            ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  1:639384/129‑1 (MQ=255)

            ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAccc                                                                                                                             <  2:956811/140‑1 (MQ=255)

            ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAccc                                                                                                                             <  2:1180455/140‑1 (MQ=255)

            ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCGAAacc                                                                                                                             >  1:585347/1‑140 (MQ=255)

            ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAccc                                                                                                                             >  1:319948/1‑140 (MQ=255)

            ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCGAAacc                                                                                                                             >  2:1509728/1‑140 (MQ=255)

            ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAccc                                                                                                                             >  1:502253/1‑140 (MQ=255)

             tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                            <  1:119487/140‑1 (MQ=255)

             tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                            <  1:791502/140‑1 (MQ=255)

                    cgcCAATCGCATCACCTTAACTATTGATAATAAAGGACATTAACCGTAGGCCGGATAAGATGCGCCAGCAGCGCATCCGGCGATGCGGGCGCGTTGAATGTGAGATCCCGTACGTTCACCGCACCCTAACCCTCTCCCCa                                                                                                                     >  2:169158/1‑140 (MQ=38)

                    cgcCAATCGCAGCACCTTAACTAGTGATCATTCAAGACATTAACCGGAGGCCGGAGAAGATGCGCCAGCATCGCATCCGTCGATGCTGGCGCGGGGGAGTTTACATCGCGTGCGGGCCCCTCCCCCTATCCCTCTccccc                                                                                                                     >  2:1467160/1‑139 (MQ=255)

                      ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                   <  1:1291992/140‑1 (MQ=255)

                      ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACACCCCcaa                                                                                                                   >  1:684167/1‑140 (MQ=255)

                          tCGCATCACCTTAACTCTGGATAATCAAAGACATGACCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCCCCCTAACCCTCGCCCCCCAgggg                                                                                                               <  2:93695/140‑1 (MQ=37)

                             cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGa                                                                                                            <  2:776630/140‑1 (MQ=255)

                             cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                            <  2:1051021/140‑1 (MQ=255)

                              aTCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        <  1:1129472/111‑1 (MQ=255)

                              aTCACCTTAACTATTGATAATAAAAGACATTAACCGGAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCGGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                        >  2:1129472/1‑111 (MQ=255)

                                  ccTTAACTATTGATAATAACAGACCTTAACCGTAGGCCGGAGAACATGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAAATTTACATCCCGTACGTTCCCCTCACCCTAACCCGCTCCCCCCAGGGGCGAGGGGa                                                                                                       <  2:726740/140‑1 (MQ=255)

                                  ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                       <  1:717197/140‑1 (MQ=255)

                                     tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCTCACCCTAAACCTCTCCCCAAAGGGGGGTGGGGTCCg                                                                                                    >  2:1457076/1‑140 (MQ=39)

                                       actactGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                  <  2:848596/140‑1 (MQ=255)

                                       acgaTTGATAATAAAAGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                  <  2:1643425/137‑1 (MQ=255)

                                       aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                  <  2:1287458/140‑1 (MQ=255)

                                       aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                  <  1:1249882/140‑1 (MQ=255)

                                       aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCTAAACCACTCCCCCAAGGGGCGAGGGGACCGtt                                                                                                  >  2:1162528/1‑140 (MQ=39)

                                           ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGTATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCTCCCCAAAGGGGCGAGGGGTCCGGTCAGt                                                                                              >  2:548969/1‑140 (MQ=255)

                                           ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCCACCCAAACCCAATCCCCCAAGGGGCCAGGGGccccgtcact                                                                                              >  2:1061798/1‑133 (MQ=37)

                                            tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTa                                                                                             <  1:1583888/140‑1 (MQ=255)

                                            tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                             <  1:215136/140‑1 (MQ=255)

                                            tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                             <  1:849701/140‑1 (MQ=255)

                                            tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAACCAAACCCAAAACCCAAACGGGCGAGGGGGCCGTTCAGTa                                                                                             >  1:252266/1‑140 (MQ=37)

                                             gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCGAACCCACTACCCCAAGGGGCGAGGGGACCCTTCAGTA‑c                                                                                           >  2:386664/1‑140 (MQ=38)

                                             gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACTACCCAAAAGGGCGAGGGGACCGGTAAGTA‑c                                                                                           >  1:980893/1‑140 (MQ=37)

                                             gATAATAAAAGACATTAACCGAAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCAAACCCACAACCCAAAGCGGCCCGGGGACCGGTCACTA‑c                                                                                           >  2:1484391/1‑140 (MQ=37)

                                                 ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGaa                                                                                       <  2:1261498/140‑1 (MQ=255)

                                                   aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAAtt                                                                                     <  1:677826/140‑1 (MQ=255)

                                                     aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCCAAACCACACCCCAAAGGGGCGAGGGGCCCCGTCAGAA‑CCGAAATTa                                                                                   >  1:187667/1‑140 (MQ=37)

                                                     aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAAACCTCAACCCCAAGGGGCGAGGGGACCGGTCAGTA‑CGGAAtttt                                                                                   >  1:862138/1‑139 (MQ=38)

                                                           ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGa                                                                             <  2:530694/140‑1 (MQ=255)

                                                           tgaaCCGTCGGCCGGATTAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTAGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGa                                                                             <  2:251347/138‑1 (MQ=255)

                                                            tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCACAACCCAAAGGGGCGAGGGGGCCGGTGAGTA‑AGGTATTTATCtttaa                                                                            >  1:204990/1‑137 (MQ=25)

                                                             aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACg                                                                           <  1:1302310/140‑1 (MQ=255)

                                                                cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTg                                                                        <  2:299450/140‑1 (MQ=255)

                                                                cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTg                                                                        <  1:1320131/140‑1 (MQ=255)

                                                                  tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCGAAACCTCACCCCAAATGGGCGGGGGGGCCCGTGAGTT‑AGGTATTTATCATGTCGGGGCt                                                                      >  1:1657187/1‑140 (MQ=21)

                                                                  tAGGCCGGATAAGATGCGCCAGCATAGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCACAACCCAAAAGGGGGAGGGGCCCCGTCAGTA‑AGGAATTTAGCATGTAGGTggt                                                                      >  1:1160987/1‑138 (MQ=25)

                                                                    ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCACACCCCAAAAGGGCCAGGGGACCGTTCACTA‑AGGAAATTAACATGAAGGTGCTaa                                                                    >  1:1782487/1‑139 (MQ=25)

                                                                    ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCACAACCCAAAGGGGCGAGGGGACCGGTCAGTA‑CGGAATTTAGCTTGACGGTGCTAt                                                                    >  2:264909/1‑140 (MQ=39)

                                                                    ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAAACCTCACCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTTACGGTGCTAt                                                                    >  2:1577002/1‑140 (MQ=255)

                                                                     gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATc                                                                   <  1:820773/140‑1 (MQ=255)

                                                                     gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATc                                                                   <  1:430723/140‑1 (MQ=255)

                                                                     gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATc                                                                   <  1:1457076/140‑1 (MQ=255)

                                                                     gCCGGATAACATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCTTACGTTCCCCACACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTT‑CGGAATTTAGCTTGACGGTGCTATc                                                                   <  2:664580/140‑1 (MQ=39)

                                                                      ccGGATCAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCACTA‑CGGAATTTAGCTTGACGGTGCTATcc                                                                  <  1:1007013/140‑1 (MQ=255)

                                                                      ccGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTTCTTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATcc                                                                  <  2:226311/140‑1 (MQ=255)

                                                                       cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGTCGGTGCTATCCa                                                                 <  1:928509/140‑1 (MQ=255)

                                                                         gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTACCCAAAGGGGCGAGGGGACCGGTCAGTA‑CGGAATTTAGCTTGACGGTGGTATCCAGc                                                               >  2:1674186/1‑140 (MQ=255)

                                                                          aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCCACCCCAACCCCCACCCCCAAGGGGCGGGGGGGCCCGTCAGTACCGGAATTT‑TCTTTAAGGGGCTATACAGCa                                                              >  1:103480/1‑140 (MQ=255)

                                                                          aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCa                                                              >  2:888761/1‑140 (MQ=255)

                                                                          aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAAACCACTACCCAAATGGGCGAGGGGACCGTTGAGTT‑CGGAATTTAGCGTGACGGTGCGATCCAGCa                                                              >  2:60145/1‑140 (MQ=255)

                                                                          aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAAACACAACCCAAATGGGGGAGGGGGCCCGTCATTA‑AGGAAAATATATTTAAGGTGCTATAAAGCa                                                              >  1:350753/1‑140 (MQ=255)

                                                                           tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCAt                                                             <  2:1296370/140‑1 (MQ=255)

                                                                            aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACAGCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                            <  2:1004733/140‑1 (MQ=255)

                                                                                 gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCACAACCCAAAGGGGCGAGGGGGCCGTTCAGTA‑AGGAAATTATCTTGTAGGTGGTATCCAGCaataaata                                                       >  1:871291/1‑140 (MQ=255)

                                                                                 gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAAACCAAACCCCAAAGGGGCGAGGGGACCGGTCAGTA‑CGGTATTTAGCTTGACGGGGCTATACAGCCATAAATa                                                       >  1:899315/1‑140 (MQ=255)

                                                                                               tcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCACTCCCCAAAGGGGCGGGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaaatctcgc                                         >  2:684332/1‑133 (MQ=255)

                                                                                                 cGGCGATGCTGGCGCGTTGACTTTTACATCCCGTACGTTCCCCGCACCCTAAACCACTCCCCAAAGGGGCGAGGGGGCCGGTCAGTA‑AGGCATTTATCATGAAGGTGCTAACCAGCATTAAATAAAGGCAAGacacacac                                       >  2:421761/1‑140 (MQ=255)

                                                                                                   gCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGc                                     >  2:1769427/1‑140 (MQ=255)

                                                                                                      aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                  <  2:1355892/140‑1 (MQ=255)

                                                                                                           ggCGCGATGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                             <  1:779343/140‑1 (MQ=255)

                                                                                                                 ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                       <  1:942902/140‑1 (MQ=255)

                                                                                                                 ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                       <  1:1642399/140‑1 (MQ=255)

                                                                                                                 ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                       <  2:745839/140‑1 (MQ=255)

                                                                                                                          aCATCCCGTACGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGGTCCGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACAGGCAAAACTCTCACGCAAAATCCGCAGCACCGTGACTTc              >  2:1754238/1‑140 (MQ=38)

                                                                                                                                cGTACGTTCCCCTCACCCAAAACCAAAACCCAAAGGGGCGAGGGGCCCCTTCAGTA‑CCGAAATTAGCGTGACGGTGCTATCCAGCAATAAAAACTGGCAAAACTCTAACGCAAAAAACCCAGCACCGTCTCATAACCGCt        >  2:1423951/1‑140 (MQ=255)

                                                                                                                                     gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt   <  1:1054096/140‑1 (MQ=255)

                                                                                                                                     gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt   <  2:1194757/140‑1 (MQ=255)

                                                                                                                                      ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt  <  2:631247/140‑1 (MQ=255)

                                                                                                                                      ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt  <  1:1478108/140‑1 (MQ=255)

GTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTA‑CGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATT  >  NC_000913/3504721‑3504994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`