BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,504,873	0	T	A	24.5%	436.1 / 41.8	247	intergenic (+90/+62)	frlR/yhfS	putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand): ref base T (44/138); new base A (57/2); total (106/141)
Fisher's exact test for biased strand distribution p-value = 8.06e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 264 total aligned reads displayed.

TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NC_000913/3504734‑3505012

tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                             <  1:845593/140‑1 (MQ=255)

tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                             <  1:787498/140‑1 (MQ=255)

tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                             <  1:345724/140‑1 (MQ=255)

tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                             <  1:1648774/140‑1 (MQ=255)

tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCACTCACCCTAACCct                                                                                                                                             <  1:1622163/140‑1 (MQ=255)

tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGAGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                             <  2:1506631/140‑1 (MQ=255)

       cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACACCCCa                                                                                                                                      >  1:1508136/1‑140 (MQ=39)

       cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCACAccccc                                                                                                                                      >  1:979692/1‑139 (MQ=38)

       cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTGCCCCACACCCAAAACCACACCCCa                                                                                                                                      >  1:871910/1‑140 (MQ=38)

             tCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgggg                                                                                                                                <  2:1707854/140‑1 (MQ=255)

                cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGa                                                                                                                             <  2:776630/140‑1 (MQ=255)

                cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                             <  2:264803/140‑1 (MQ=255)

                cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                             <  2:1051021/140‑1 (MQ=255)

                cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                             <  2:1079181/140‑1 (MQ=255)

                     ccTTCACTCTTGAGAATCAAAGACCGTCCCCGTCGGCCGGACAAGAGGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGa                                                                                                                        <  2:1537832/140‑1 (MQ=37)

                     ccTTACCTCTTGAGACTACAAGCCATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTGACATCCCGTACGGTCCCCTCACCCTCACCCTCTCCCCCCCGGGGCGAGGGGa                                                                                                                        <  1:996263/140‑1 (MQ=37)

                     ccTTACCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGa                                                                                                                        <  1:1434254/140‑1 (MQ=255)

                     ccTTAACTCTTGATAATCAAAGACCGTAACCGTAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGACTTTTACATCCCGTACGTTCCCCACACCCTAACCCTCGCCCCAAAGGGGCGAGGGGa                                                                                                                        <  2:1329332/140‑1 (MQ=38)

                     ccTTAACTCTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                        <  1:192805/140‑1 (MQ=255)

                     ccTTAACTATTGATAATAACAGACCTTAACCGTAGGCCGGAGAACATGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAAATTTACATCCCGTACGTTCCCCTCACCCTAACCCGCTCCCCCCAGGGGCGAGGGGa                                                                                                                        <  2:726740/140‑1 (MQ=255)

                     ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                        <  1:717197/140‑1 (MQ=255)

                          actactGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                   <  2:848596/140‑1 (MQ=255)

                          actactCATACTAACCGACAATAACCGTAGGCCCGATAACATGCGCCCGCCTCGCACCCGGCGATGCGGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCACCCCACCGGGGCGAGGGGACCGtt                                                                                                                   <  2:80052/140‑1 (MQ=25)

                          acgaTTGATAATAAAAGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                   <  2:1643425/137‑1 (MQ=255)

                          aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                   <  2:101266/140‑1 (MQ=255)

                            tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCa                                                                                                                 <  1:654193/140‑1 (MQ=255)

                              ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGTATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCTCCCCAAAGGGGCGAGGGGTCCGGTCAGt                                                                                                               >  2:548969/1‑140 (MQ=255)

                               tGATAATAAAAGACATTAACCGTAGGCCGGATAATATGCGCCAGCATCGCATCCGGCGAAGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTCCCCAAAGTGGCGAGGTGACCGTACATTa                                                                                                              >  2:1777108/1‑140 (MQ=38)

                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                              <  1:1582548/140‑1 (MQ=255)

                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                              <  1:215136/140‑1 (MQ=255)

                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                              <  1:849701/140‑1 (MQ=255)

                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                              <  1:415752/140‑1 (MQ=255)

                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAACCAAACCCAAAACCCAAACGGGCGAGGGGGCCGTTCAGTa                                                                                                              >  1:252266/1‑140 (MQ=37)

                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGTCCGTTCAGTAc                                                                                                             >  2:894666/1‑140 (MQ=255)

                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTACCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                             >  2:1682911/1‑140 (MQ=255)

                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACCCCCAAACCCACAACCCCAAAGGGCGAGGGGACCGTTAAGTaa                                                                                                             >  1:1296472/1‑139 (MQ=37)

                                  taataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACgg                                                                                                           <  1:864869/140‑1 (MQ=255)

                                    ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                                         <  2:944615/140‑1 (MQ=255)

                                    ataaAAGACATTAACCGAAGGCCGGATAAGATGCGCCAGCATCGCATCCCGCGATGCTGGCGCCAAGAATTTTACATCCCGAACCTTCCCCTCACCCTAACCCACACCCCAAACCGGCGAGGGGACCGTTCAGTACGGaa                                                                                                         <  2:1672382/140‑1 (MQ=37)

                                      aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTATCCCTCTACCCAAAGGGGCGAGGGGACCGTTCAGTAAGGaaat                                                                                                       >  2:1357502/1‑138 (MQ=39)

                                        aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCCAAACCACACCCCAAAGGGGCGAGGGGCCCCGTCAGAACCGAAATTa                                                                                                     >  1:187667/1‑140 (MQ=37)

                                            cATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCtt                                                                                                 <  2:1060142/140‑1 (MQ=255)

                                             aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCACTACCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGcatt                                                                                                >  1:679468/1‑137 (MQ=39)

                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:530694/140‑1 (MQ=255)

                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:1139870/140‑1 (MQ=255)

                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:1738477/140‑1 (MQ=255)

                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:1480427/140‑1 (MQ=255)

                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:1702381/140‑1 (MQ=255)

                                              tgaaCCGTCGGCCGGATTAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTAGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:251347/138‑1 (MQ=255)

                                               tAACCGTAGGCCGGTTAAGATGCGCCAGCATCGCATCCGGGGATGCTGGCGCGTTGGATTTTACATCCCGTACGGTCCCCGCACCCCTAACCTCTCCCCCTAGGGGCGCGGGGGCCGTGCAGTACGGAATTTAGCTTGAc                                                                                              >  2:387864/1‑140 (MQ=37)

                                                aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACg                                                                                             <  1:1304026/140‑1 (MQ=255)

                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  2:1294030/140‑1 (MQ=255)

                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  2:445775/140‑1 (MQ=255)

                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  2:182843/140‑1 (MQ=255)

                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  1:1200945/140‑1 (MQ=255)

                                                     tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCACACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGca                                                                                        >  2:287035/1‑139 (MQ=255)

                                                     tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGCCCCCCAACCCACTCCCCAAACGGGCGGGGGGACCCGTCAGTACGGAAATTAGCGTGGCGCTGcg                                                                                        >  1:804394/1‑139 (MQ=25)

                                                     tAGGCCGGATAAGATGCGCCAGCATAGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCACAACCCAAAAGGGGGAGGGGCCCCGTCAGTAAGGAATTTAGCATGTAGGTggt                                                                                        >  1:1160987/1‑138 (MQ=25)

                                                       ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCACACCCCAAAAGGGCCAGGGGACCGTTCACTAAGGAAATTAACATGAAGGTGCTaa                                                                                      >  1:1782487/1‑139 (MQ=25)

                                                       ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAAACCTCACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTTACGGTGCTAt                                                                                      >  2:1577002/1‑140 (MQ=255)

                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                     <  2:470638/140‑1 (MQ=255)

                                                        gCCGGATAACATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCTTACGTTCCCCACACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTTCGGAATTTAGCTTGACGGTGCTATc                                                                                     <  2:664580/140‑1 (MQ=39)

                                                         ccGGATCAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCACTACGGAATTTAGCTTGACGGTGCTATcc                                                                                    <  1:1007013/140‑1 (MQ=255)

                                                          cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGTCGGTGCTATCCa                                                                                   <  1:928509/140‑1 (MQ=255)

                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACGGTGCGATCCAGCa                                                                                >  2:718258/1‑140 (MQ=255)

                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCAAAGGGGCGAGGGGCCCGGTCAGTAAGGAATTTATCATGACGGTGCTTACCAGCa                                                                                >  2:1285022/1‑140 (MQ=255)

                                                                    gcgcCAGCTTCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTCCGTTCCCCACAACCTAAACCTCTCCCCAAAGGGGCGAGGGGACCCGTCATTACCGATGTTAGCTTGTCGGTGCTATCCAGCATTaaaaa                                                                         >  2:1475902/1‑138 (MQ=255)

                                                                     cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                        <  1:607328/140‑1 (MQ=255)

                                                                      gcCCGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACGCCCGAACCCGCTCCCCCAACGGGCGCGGGGTCCGGGCGCGAAGGTAATTAGCGTGACGGGGCGATCCCGCCTTAAATACt                                                                       >  2:1464573/1‑140 (MQ=255)

                                                                      gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                                       <  1:103194/140‑1 (MQ=255)

                                                                        cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                     <  1:703651/140‑1 (MQ=255)

                                                                           caacgcatcCGGCGATGCTGGCGCGTTGACATTTAAATCCCGTACGTTGCCCGCACCCTAACCCAGACCCCCAAGTGGCGCTGGGGACGTTGAGTACGGAATATAGAAATACGGTTCAATCCAGCATTAAATAATgtgaa                                                                  >  2:1157698/4‑136 (MQ=255)

                                                                                catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGTGGGGACCGGTCCGTACGGAATTTATCTTGTAGGTGGTATCCAGCATTAAAAACTGGCCATAcgc                                                             >  2:159987/1‑138 (MQ=37)

                                                                                  tcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCTCACCCCCAAGGGGCGAGGGGGCCGTTCAGTACGGAATTTAGGTTGTCGGTGCTATACAGCAATTAATACTTGCAATAAtctc                                                           >  2:220848/1‑140 (MQ=255)

                                                                                         aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                    <  1:770130/140‑1 (MQ=255)

                                                                                              ggCGCGATGCATTTTACATCCCGTACGTGCCCCTCAGCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                               <  1:465274/140‑1 (MQ=39)

                                                                                               gcgcGTTGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgc                                              <  2:1188308/140‑1 (MQ=255)

                                                                                                  cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagc                                           <  2:583910/140‑1 (MQ=255)

                                                                                                    ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                         <  2:745839/140‑1 (MQ=255)

                                                                                                    ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                         <  1:942902/140‑1 (MQ=255)

                                                                                                             aCATCCCGTACGTTCCCCTCACCCTAACCCTCTACCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGACGGTGCTATCCAGCATTAAATACAGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCATc                                >  2:1754269/1‑140 (MQ=255)

                                                                                                                   cGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCt                          <  2:1424983/140‑1 (MQ=255)

                                                                                                                   cGTACGTTCCCCACACCCAAAACCACAACCCAAATGGGCGATGGGACCGTTCAGTACGGAAATTATCTTTACGGTGCTAAACAGCAATAAAAACAGGCAAAACTCTAACGCAAAATCACCAGCACCGTCTCTTCAACGCt                          >  2:61036/1‑140 (MQ=255)

                                                                                                                   cGTACGTTACCCTCACCCCAAACCACTCCCCAAATGGGCGGGGGGACCCGTCAGTACGGAATTTAGCTTGAACGTGCTAACCAGCCATAAATAATTGCAAAACACTCAAGCCAAAAACGCCGCACCGTCTCTTCAccccc                          >  2:1423509/1‑137 (MQ=255)

                                                                                                                        gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt                     <  2:1194757/140‑1 (MQ=255)

                                                                                                                        gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt                     <  1:1274464/140‑1 (MQ=255)

                                                                                                                         ttCCCCTCACCCTCACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt                    <  2:631321/140‑1 (MQ=255)

                                                                                                                         gtCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt                    <  1:1555451/139‑1 (MQ=255)

                                                                                                                              caaaCCCAAAACCACACCCCAAAGGGGCGAGGGGACCCTTCAGTACCGAATTTAGCTTGACGGTGCTAACCAGCATTAAATACTGGCAATACTCTCACCCAAAAAACGCAGCACCGACACTTCACCGCTGCGATTCGGGt               >  2:1521083/4‑140 (MQ=255)

                                                                                                                                  cccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt           <  1:375073/140‑1 (MQ=255)

                                                                                                                                      aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  2:345216/1‑140 (MQ=255)

                                                                                                                                      aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:1464254/1‑140 (MQ=255)

                                                                                                                                      aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:13758/1‑140 (MQ=255)

                                                                                                                                      aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:1049333/1‑140 (MQ=255)

                                                                                                                                      aaCCCTCTCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:109105/1‑140 (MQ=255)

                                                                                                                                       aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa      >  1:1676206/1‑140 (MQ=255)

                                                                                                                                       aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa      >  2:1164090/1‑140 (MQ=255)

                                                                                                                                       aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa      <  1:1107221/140‑1 (MQ=255)

                                                                                                                                       aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa      >  2:929902/1‑140 (MQ=255)

                                                                                                                                           tctcCCCAAAGGGGCGAGGGGACCGTGCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCTCCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  2:303864/1‑140 (MQ=255)

TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NC_000913/3504734‑3505012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`