Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,633,657 C→T C30Y (TGC→TAC)  yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,633,6570CT98.8% 277.1 / ‑4.3 84C30Y (TGC→TAC) yjjXnon‑canonical purine NTP phosphatase, ITPase/XTPase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (42/41);  total (43/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAA  >  NC_000913/4633530‑4633793
                                                                                                                               |                                                                                                                                        
ccAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGc                                                                                                                              <  2:157370/140‑1 (MQ=255)
     aaTCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAg                                                                                                                         <  1:1317445/140‑1 (MQ=255)
         aGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGAtct                                                                                                                     <  1:1252804/140‑1 (MQ=255)
         aGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGAtct                                                                                                                     <  1:1577346/140‑1 (MQ=255)
          gCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCACTATGGTAGGATCCTTCGCCGAAGAtctc                                                                                                                    >  2:800745/1‑140 (MQ=255)
          gCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGAtctc                                                                                                                    >  2:126725/1‑140 (MQ=255)
          gCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGAtctc                                                                                                                    >  2:274101/1‑140 (MQ=255)
          gCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGAtctc                                                                                                                    >  2:800351/1‑140 (MQ=255)
          gCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGAtctc                                                                                                                    >  2:751475/1‑140 (MQ=255)
          gCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGAtctc                                                                                                                    >  1:1719696/1‑140 (MQ=255)
              ctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGa                                                                                                                <  1:232959/140‑1 (MQ=255)
              ctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGa                                                                                                                <  1:230240/140‑1 (MQ=255)
              ctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGa                                                                                                                <  2:1283686/140‑1 (MQ=255)
                 gAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAAt                                                                                                             <  2:217821/140‑1 (MQ=255)
                 gAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAAt                                                                                                             <  2:1215509/140‑1 (MQ=255)
                 gAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAAt                                                                                                             <  2:1605921/140‑1 (MQ=255)
                       aaCGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGc                                                                                                       >  2:1412026/1‑140 (MQ=255)
                       aaCGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCa                                                                                                      >  2:1412338/1‑140 (MQ=255)
                                         cccGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTa                                                                                     >  2:1324449/1‑140 (MQ=255)
                                          ccGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAg                                                                                    <  2:848651/140‑1 (MQ=255)
                                             aTTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCgg                                                                                 >  2:533598/1‑140 (MQ=255)
                                               ttCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGa                                                                               >  1:503189/1‑140 (MQ=255)
                                               ttCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGa                                                                               >  1:53971/1‑140 (MQ=255)
                                               ttCGTGCGCCAGCGCGCGTTACCTCACTGCCAAAGGGCTGTTCCGGAACACCGCTCTCGACGGCAACGGAAGCAATAAGGTAGGATCCTTCGCCGAAGAACTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGa                                                                               >  1:54558/1‑140 (MQ=255)
                                                   tGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtgg                                                                           <  2:1443471/140‑1 (MQ=255)
                                                   tGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtgg                                                                           <  2:1443420/140‑1 (MQ=255)
                                                         aGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCg                                                                     <  1:937211/140‑1 (MQ=255)
                                                         aGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCg                                                                     <  2:1516400/140‑1 (MQ=255)
                                                         aGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCg                                                                     <  2:1407926/140‑1 (MQ=255)
                                                         aGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCg                                                                     <  2:279909/140‑1 (MQ=255)
                                                            gcgcgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGcac                                                                  <  1:357837/140‑1 (MQ=255)
                                                             cgcgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGcaca                                                                 <  1:1238583/140‑1 (MQ=255)
                                                               cgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGa                                                               <  1:1655179/140‑1 (MQ=255)
                                                                    ccTCACTGCCAAAGGGCTGTTCCGTTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACt                                                          <  2:634448/140‑1 (MQ=255)
                                                                    ccTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACt                                                          <  2:1173362/140‑1 (MQ=255)
                                                                    ccTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACt                                                          <  2:1173376/140‑1 (MQ=255)
                                                                     cTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACtt                                                         <  2:1584215/140‑1 (MQ=255)
                                                                          tGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGc                                                    >  1:390400/1‑140 (MQ=255)
                                                                          tGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGc                                                    >  2:22029/1‑140 (MQ=255)
                                                                          tGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGc                                                    >  1:1099325/1‑140 (MQ=255)
                                                                            ccAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCAt                                                  >  2:1127961/1‑140 (MQ=255)
                                                                               aaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCACTATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAAt                                               <  1:1646844/140‑1 (MQ=255)
                                                                               aaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAAt                                               <  1:56636/140‑1 (MQ=255)
                                                                               aaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAAt                                               <  1:1357505/140‑1 (MQ=255)
                                                                               aaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAAt                                               <  1:1241434/140‑1 (MQ=255)
                                                                               aaGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAAt                                               <  1:1222427/140‑1 (MQ=255)
                                                                                       ttCCGGTACACCGCTCTCGACGGCAACGGAAGCAAGATGGTAGGATCCTTCGACGAAGATCTCGTGACATCCCGGCATAATGGCCTGAATTTTATCGGGATTGGTGGTCGCACAGACACCTTGGTCCATAATCAGCagaa                                       >  2:901253/1‑137 (MQ=255)
                                                                                           ggTACACCGCTCTCGACGGCAACGGATGCAATATTCTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCa                                   <  2:683572/140‑1 (MQ=255)
                                                                                           ggTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCa                                   <  2:591844/140‑1 (MQ=255)
                                                                                            gTACACCGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCAGAAGATCTCTTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTAGAACATACAACTTGGTGCATAATCAGCATTACTCAg                                  >  1:1178032/1‑140 (MQ=255)
                                                                                                 ccGCTCTCGACGGCAACGGATGCACTATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCAGCAGATTGGCCGGAATTTAAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAt                             >  2:1079460/1‑140 (MQ=255)
                                                                                                 ccGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGTAAAt                             >  2:1675853/1‑140 (MQ=255)
                                                                                                 ccGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAt                             >  1:1225394/1‑140 (MQ=255)
                                                                                                 ccGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAt                             >  2:578932/1‑140 (MQ=255)
                                                                                                 ccGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAt                             >  2:569917/1‑140 (MQ=255)
                                                                                                 ccGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCAGAATTTTAGCGGGATTGGTGTGCGCACAGACAACTTGGAGCATAATCAGCATTACTCAGAAAAt                             >  2:1532890/1‑140 (MQ=255)
                                                                                                 ccGCTCTCGACGGCAACGGAAGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGTCTTTTAGCGGGATTGGTGGTCGCAAAGACTACTTGGTGCATAATCAGCTTTACTCAGAAAAt                             >  2:1675985/1‑140 (MQ=255)
                                                                                                  cGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAtt                            >  1:1142465/1‑140 (MQ=255)
                                                                                                  cGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAtt                            >  2:1441730/1‑140 (MQ=255)
                                                                                                  cGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAtt                            >  2:160064/1‑140 (MQ=255)
                                                                                                  cGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAtt                            <  2:516588/140‑1 (MQ=255)
                                                                                                  cGCTCTCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAtt                            >  2:255391/1‑140 (MQ=255)
                                                                                                  cGCTCTCGACGGCAACGGATGCAATAGGGTAGGATCCTTCGCCGAAGATCTCGTGAAAAGCCTGCAGAATGGCCTGAATTTTAGCGGGAGTGGTGGTCGCACCGACACCTTGGTGCAGTATCAGCTTTCCGCAGaagagt                            >  1:1142461/1‑136 (MQ=255)
                                                                                                    ctctctACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACtt                                                         >  1:1177726/1‑109 (MQ=255)
                                                                                                    ctctctACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACtt                                                         <  2:1177726/109‑1 (MQ=255)
                                                                                                    ctctCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGATATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTaa                          <  2:1229541/140‑1 (MQ=255)
                                                                                                    ctctCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTaa                          >  1:519082/1‑140 (MQ=255)
                                                                                                    ctctCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTaa                          >  2:471170/1‑140 (MQ=255)
                                                                                                    ctctCGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTaa                          >  1:689434/1‑140 (MQ=255)
                                                                                                    ctctCGACGGCAACGGATGCAAGATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTGAGCGGGAGTGGAGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTaa                          >  2:811222/1‑140 (MQ=255)
                                                                                                       tcGACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGTTTGGTGGTCGCACAGAGAACTTGGTGCTTAATCAGCATTACTCAGAAAATTAACGt                       <  2:1545067/140‑1 (MQ=255)
                                                                                                         gACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTa                     >  1:632589/1‑140 (MQ=255)
                                                                                                         gACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTa                     <  2:1359014/140‑1 (MQ=255)
                                                                                                         gACGGCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTa                     <  2:452852/140‑1 (MQ=255)
                                                                                                         gACGGCAACGGATGCAATATGGTAGGATCCTTCGACGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGTTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATGAACGTTa                     >  1:630230/1‑140 (MQ=255)
                                                                                                            ggCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcag                  <  1:1298624/140‑1 (MQ=255)
                                                                                                            ggCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcag                  <  2:1380100/140‑1 (MQ=255)
                                                                                                             gCAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcagc                 <  1:385502/140‑1 (MQ=255)
                                                                                                              cAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcagca                >  2:16346/1‑140 (MQ=255)
                                                                                                              cAACGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcagca                <  2:183656/140‑1 (MQ=255)
                                                                                                                 cGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGta             >  1:549514/1‑140 (MQ=255)
                                                                                                                 cGGATGCAATATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGta             >  1:1277265/1‑140 (MQ=255)
                                                                                                                        aaTATGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACGCAGAAAATTAACGTTACAGCAGTATACGGaa      >  2:783041/1‑140 (MQ=255)
                                                                                                                            tGGTAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGaaaaaa  >  1:1554469/1‑140 (MQ=255)
                                                                                                                               |                                                                                                                                        
CCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGCAGTATACGGAAAAAA  >  NC_000913/4633530‑4633793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: