Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,463,322	A→C	I400L (ATT→CTT)	paaK →	phenylacetyl‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,463,322	0	A	C	92.5%	269.7 / 6.4	81	I400L (ATT→CTT)	paaK	phenylacetyl‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/6);  new base C (33/41);  total (33/48)
Fisher's exact test for biased strand distribution p-value = 3.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGG  >  NC_000913/1463185‑1463459                                                                                                                                          |                                                                                                                                          tATCGGCGCATTACCAACTGGAGGTGAACCGCCGTGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTTAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCtt                                                                                                                                         <  2:458747/140‑1 (MQ=255)        gcATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGa                                                                                                                                  >  2:1261947/1‑140 (MQ=255)        gcATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGa                                                                                                                                  >  2:1574707/1‑140 (MQ=255)        gcATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGa                                                                                                                                  <  1:1225346/140‑1 (MQ=255)        gcATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGa                                                                                                                                  <  1:1222046/140‑1 (MQ=255)             aCCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGATAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGGTTAAGTCGAtggtg                                                                                                                             <  2:270754/140‑1 (MQ=255)             aCCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGAtggtg                                                                                                                             <  1:236775/140‑1 (MQ=255)               cAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTggg                                                                                                                           <  2:676421/140‑1 (MQ=255)                 aCTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGa                                                                                                                         >  1:583644/1‑140 (MQ=255)                  cTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCAGCGCCATCGGCTTAAGTCGATGGTGGGGAt                                                                                                                        <  1:144643/140‑1 (MQ=255)                       ggTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTa                                                                                                                   <  1:1547524/140‑1 (MQ=255)                        gTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGTAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTAc                                                                                                                  >  1:1118443/1‑140 (MQ=255)                        gTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTAc                                                                                                                  >  1:1119004/1‑140 (MQ=255)                        gTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTAc                                                                                                                  >  1:29047/1‑140 (MQ=255)                          gAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCg                                                                                                                >  1:1555088/1‑140 (MQ=255)                          gAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCg                                                                                                                >  1:841930/1‑140 (MQ=255)                           aaCCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGa                                                                                                               <  2:1195751/140‑1 (MQ=255)                             ccgccgGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGAtg                                                                                                             >  1:1080365/1‑140 (MQ=255)                                ccgGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGtga                                                                                                          <  1:1419715/140‑1 (MQ=255)                                ccgGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGtga                                                                                                          <  1:453519/140‑1 (MQ=255)                                ccgGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGtga                                                                                                          <  1:1217522/140‑1 (MQ=255)                                 cgGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGtgat                                                                                                         <  2:1641038/140‑1 (MQ=255)                                     ggCATCTTGATTCACTTTCTGTGAAATTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTGAAGTCGATGGTGGGGATCTCTACCGATGTGATGATc                                                                                                     >  2:1008053/1‑140 (MQ=255)                                      gCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCg                                                                                                    <  2:1042239/140‑1 (MQ=255)                                      gCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCg                                                                                                    <  2:1043714/140‑1 (MQ=255)                                        aTCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGtt                                                                                                  <  1:521057/140‑1 (MQ=255)                                        aTCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGtt                                                                                                  <  1:685379/140‑1 (MQ=255)                                        aTCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGtt                                                                                                  <  1:866506/140‑1 (MQ=255)                                        aTCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGtt                                                                                                  <  1:1480204/140‑1 (MQ=255)                                        aTCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGtt                                                                                                  <  1:1455267/140‑1 (MQ=255)                                        aTCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGtt                                                                                                  <  1:1408877/140‑1 (MQ=255)                                        aTCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGtt                                                                                                  <  2:368565/140‑1 (MQ=255)                                        aTCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGtt                                                                                                  <  2:517175/140‑1 (MQ=255)                                          cTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTaa                                                                                                >  1:832278/1‑140 (MQ=255)                                             gATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACtg                                                                                             <  1:1071625/140‑1 (MQ=255)                                             gATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACtg                                                                                             <  1:504620/140‑1 (MQ=255)                                                  aCTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTTACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCAACCGAGGTGATCATCGTTAACTGTGTCa                                                                                        >  1:1061107/1‑140 (MQ=255)                                                  aCTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGAGATGATCGTTAACTGTGGCa                                                                                        >  1:1061090/1‑140 (MQ=255)                                                              aGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGtt                                                                            >  1:481286/1‑140 (MQ=255)                                                                tGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCa                                                                          >  1:908041/1‑140 (MQ=255)                                                                 ggAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAg                                                                         <  2:180602/140‑1 (MQ=255)                                                                            aaagTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAATGCg                                                              >  2:1437568/1‑140 (MQ=255)                                                                               gTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCgtgt                                                           >  2:983820/1‑140 (MQ=255)                                                                               gTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCgtgt                                                           >  1:1737570/1‑140 (MQ=255)                                                                                 aGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCg                                                         <  1:866733/140‑1 (MQ=255)                                                                                   ccTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCggg                                                       >  1:1166283/1‑140 (MQ=255)                                                                                       aCATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGgtggt                                                   >  1:1355367/1‑138 (MQ=255)                                                                                           tGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGAt                                               <  1:517141/140‑1 (MQ=255)                                                                                           tGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGAt                                               <  1:860698/140‑1 (MQ=255)                                                                                           tGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGAt                                               <  2:1273505/140‑1 (MQ=255)                                                                                            gACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATc                                              >  2:687994/1‑140 (MQ=255)                                                                                                cATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTgcg                                          <  1:91873/140‑1 (MQ=255)                                                                                                cATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTgcg                                          <  1:890658/140‑1 (MQ=255)                                                                                                cATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTgcg                                          <  1:61633/140‑1 (MQ=255)                                                                                                cATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTgcg                                          <  1:43232/140‑1 (MQ=255)                                                                                                 aTGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTgcgc                                         <  1:94192/140‑1 (MQ=255)                                                                                                          gTTGCTAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATAttgtt                                <  1:1050376/140‑1 (MQ=255)                                                                                                          gTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATAttgtt                                <  1:1050459/140‑1 (MQ=255)                                                                                                          gTTGCCAGGTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATAttgtt                                <  2:240146/140‑1 (MQ=255)                                                                                                          gTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATAttgtt                                <  2:783547/140‑1 (MQ=255)                                                                                                                 ggTATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCt                         <  1:301527/140‑1 (MQ=255)                                                                                                                   tATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGtga                                                                                                          >  2:1577928/1‑57 (MQ=255)                                                                                                                   tATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGtga                                                                                                          <  1:1577928/57‑1 (MQ=255)                                                                                                                   tATGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGa                       >  1:1692294/1‑140 (MQ=255)                                                                                                                     tGCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACg                     >  1:137108/1‑140 (MQ=255)                                                                                                                      gCCACCAGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGt                    >  1:1497846/1‑140 (MQ=255)                                                                                                                       ccaccaGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTa                   >  2:1646630/1‑140 (MQ=255)                                                                                                                       ccaccaGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTa                   >  2:1646878/1‑140 (MQ=255)                                                                                                                       ccaccaGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTa                   <  1:1271557/140‑1 (MQ=255)                                                                                                                       ccaccaGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTa                   <  1:1271519/140‑1 (MQ=255)                                                                                                                        caccaGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTAt                  >  2:342996/1‑140 (MQ=255)                                                                                                                            aGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTc              >  2:1452965/1‑140 (MQ=255)                                                                                                                            aGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTc              <  1:1446560/140‑1 (MQ=255)                                                                                                                            aGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTc              >  2:1202367/1‑140 (MQ=255)                                                                                                                            aGCTGCGCCATCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTc              >  2:935682/1‑140 (MQ=255)                                                                                                                                  gcCATCGGCTTAAGTCGATGGAGGGGATCTCTACCGATGTGATGATCGTTACCTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGccc        <  2:1762920/140‑1 (MQ=255)                                                                                                                                      tCGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCtggt    <  1:412441/140‑1 (MQ=255)                                                                                                                                       cGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCtggtg   >  1:597458/1‑140 (MQ=255)                                                                                                                                       cGGCTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCtggtg   >  1:273770/1‑140 (MQ=255)                                                                                                                                        ggATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCtggtgg  <  1:104854/140‑1 (MQ=255)                                                                                                                                        ggATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCtggtgg  <  1:104721/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                          TATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGG  >  NC_000913/1463185‑1463459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑