Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 222,958 Δ1 bp 100% coding (126/576 nt) gmhB → D,D‑heptose 1,7‑bisphosphate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913222,9580A.99.3% 1479.8 / 1.3 302coding (126/576 nt)gmhBD,D‑heptose 1,7‑bisphosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base . (133/167);  total (135/167)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 320 total aligned reads displayed.

CCTTATTAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTATCTA  >  NC_000913/222811‑223101
                                                                                                                                                   |                                                                                                                                               
ccTTATTAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaa                                                                                                                                               <  2:1331020/150‑1 (MQ=255)
  ttattaAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaa                                                                                                                                             <  2:1454768/150‑1 (MQ=255)
    attaAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaaaa                                                                                                                                           >  2:4816324/1‑150 (MQ=255)
     ttaAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaaaat                                                                                                                                          <  2:2199379/150‑2 (MQ=255)
     ttaAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaaaat                                                                                                                                          <  2:4665301/150‑2 (MQ=255)
     ataAGGAGCTATAACAGGCGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTAGGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGAAGCCATGCGCGAGCTaaaaaaat                                                                                                                                          <  2:4762651/149‑2 (MQ=255)
        aGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaaaatggg                                                                                                                                       <  1:251649/150‑5 (MQ=255)
            gCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCttt                                                                                                                                  >  2:2000812/1‑150 (MQ=255)
               ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTgcg                                                                                                                               >  1:4805270/1‑150 (MQ=255)
                taAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTgcgc                                                                                                                              >  2:1894981/1‑150 (MQ=255)
                  aaaGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCtg                                                                                                                            <  1:1029879/150‑1 (MQ=255)
                    aGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCtggt                                                                                                                          >  2:3553939/1‑150 (MQ=255)
                       tGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTggtggt                                                                                                                       >  2:2430039/1‑150 (MQ=255)
                       tGGCGAAGAGCGTACCCGCAATTTTTCTAGACCGTGAAGACACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTAATCGACGGTGATATTGACGCCATGCGCGAGCTAAAAAAAAGGGCTGTGCGCTggtggt                                                                                                                       >  2:2964731/1‑150 (MQ=255)
                        ggCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTCTCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGgta                                                                                                                      <  2:3369502/150‑1 (MQ=255)
                        ggCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGgta                                                                                                                      <  1:1705751/150‑1 (MQ=255)
                          cGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGgtagt                                                                                                                    <  1:2792434/150‑1 (MQ=255)
                          cGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGgtagt                                                                                                                    <  2:1010822/150‑1 (MQ=255)
                           gAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGgtagta                                                                                                                   >  2:4741908/1‑150 (MQ=255)
                           gAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGgtagta                                                                                                                   >  2:3162663/1‑150 (MQ=255)
                            aaGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTaa                                                                                                                  <  1:4937889/150‑1 (MQ=255)
                             agagCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGTTCACGGCTATGTCCATGAGATCGACAACTTTTAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAATAATGGGCTTTGCGCTGGTGGTAGTaac                                                                                                                 <  1:3790639/150‑1 (MQ=255)
                                gCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTaaccaa                                                                                                              <  1:3144241/150‑1 (MQ=255)
                                gCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTaaccaa                                                                                                              <  1:1629262/150‑1 (MQ=255)
                                gCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTaaccaa                                                                                                              <  2:1231849/150‑1 (MQ=255)
                                gCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTaaccaa                                                                                                              <  2:1155708/150‑1 (MQ=255)
                                 cGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCTTGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTaaccaac                                                                                                             <  2:4912580/150‑1 (MQ=255)
                                   tACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTTACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAaccaacca                                                                                                           >  2:1792388/1‑150 (MQ=255)
                                   tACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAaccaacca                                                                                                           >  2:3998749/1‑150 (MQ=255)
                                   tACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAaccaacca                                                                                                           >  1:150757/1‑150 (MQ=255)
                                    aCCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAg                                                                                                          >  1:5112948/1‑150 (MQ=255)
                                       cGCACTTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCGTTGCGCTGGTGGTCGTAACCCCCCCGTCt                                                                                                       >  2:887059/1‑150 (MQ=255)
                                        gCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTg                                                                                                      >  1:5083319/1‑150 (MQ=255)
                                         cAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGTGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTgg                                                                                                     >  1:4456146/1‑150 (MQ=255)
                                                  ttGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTcg                                                                                            >  2:2860580/1‑150 (MQ=255)
                                                     aCCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCgg                                                                                         >  2:2759829/1‑150 (MQ=255)
                                                     aCCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCgg                                                                                         <  1:588447/150‑1 (MQ=255)
                                                       cGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTa                                                                                       >  1:4327471/1‑150 (MQ=255)
                                                         tGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTaaa                                                                                     <  2:5115660/150‑1 (MQ=255)
                                                         tGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTaaa                                                                                     <  1:3460621/150‑1 (MQ=255)
                                                             ggCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTa                                                                                 >  1:1105396/1‑150 (MQ=255)
                                                              gCACCATTACTGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTAc                                                                                <  1:2000279/150‑1 (MQ=255)
                                                                aCCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCg                                                                              <  1:3089651/150‑1 (MQ=255)
                                                                 ccATTATTGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGa                                                                             <  2:3318397/150‑1 (MQ=255)
                                                                  cATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGaa                                                                            <  1:4752411/150‑1 (MQ=255)
                                                                   aTTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAg                                                                           <  1:4840218/150‑1 (MQ=255)
                                                                        tGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAg                                                                      >  2:4053763/1‑150 (MQ=255)
                                                                         gTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGt                                                                     <  1:962799/150‑1 (MQ=255)
                                                                           cGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGACGCACAGttg                                                                   >  1:4384005/1‑149 (MQ=255)
                                                                            gATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTg                                                                  <  1:2209312/150‑1 (MQ=255)
                                                                             aTCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGa                                                                 >  1:1246896/1‑150 (MQ=255)
                                                                               cACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGaaa                                                               <  2:2695565/150‑1 (MQ=255)
                                                                                 cGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTTCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGAAAATTTACCGAAGCACAGTTTGAAACg                                                             >  2:42502/1‑150 (MQ=255)
                                                                                 cGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACg                                                             >  2:2614433/1‑150 (MQ=255)
                                                                                  ggCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGc                                                            <  2:635853/150‑1 (MQ=255)
                                                                                   gCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCt                                                           <  2:4535243/150‑1 (MQ=255)
                                                                                   gCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCt                                                           <  1:887059/150‑1 (MQ=255)
                                                                                        gTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCg                                                      >  2:3460632/1‑150 (MQ=255)
                                                                                          ccATGAGATCGACAACTTTGAATTTATCGCCGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAg                                                    <  2:4949227/150‑1 (MQ=255)
                                                                                           cATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGt                                                   >  1:1917049/1‑150 (MQ=255)
                                                                                            aTGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtg                                                  >  2:2311076/1‑150 (MQ=255)
                                                                                            aTGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtg                                                  <  2:752245/150‑1 (MQ=255)
                                                                                             tGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtgg                                                 <  1:4150084/150‑1 (MQ=255)
                                                                                             tGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtgg                                                 >  2:2034291/1‑150 (MQ=255)
                                                                                             tGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtgg                                                 >  1:2716529/1‑150 (MQ=255)
                                                                                             tGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtgg                                                 <  1:3686538/150‑1 (MQ=255)
                                                                                               agaTCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTTAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtggat                                               <  2:3860205/150‑1 (MQ=255)
                                                                                               agaTCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtggat                                               >  2:4795842/1‑150 (MQ=255)
                                                                                                 aTCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtggatgg                                             >  1:1826361/1‑150 (MQ=255)
                                                                                                    gACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACt                                          >  2:4603217/1‑150 (MQ=255)
                                                                                                    gACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACt                                          <  1:1586952/150‑1 (MQ=255)
                                                                                                    gACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACt                                          <  1:738153/150‑1 (MQ=255)
                                                                                                          tttGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGc                                    >  1:404296/1‑150 (MQ=255)
                                                                                                          tttGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGc                                    >  1:141190/1‑150 (MQ=255)
                                                                                                          tttGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGc                                    >  1:558503/1‑150 (MQ=255)
                                                                                                            tGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTg                                  <  1:4706867/150‑1 (MQ=255)
                                                                                                              aaTTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGc                                >  2:803208/1‑150 (MQ=255)
                                                                                                               aTTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCg                               <  1:3004399/150‑1 (MQ=255)
                                                                                                                 ttATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGa                             >  1:3580526/1‑150 (MQ=255)
                                                                                                                  tATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGAc                            <  2:1674866/150‑1 (MQ=255)
                                                                                                                  tATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGAc                            >  2:1856035/1‑150 (MQ=255)
                                                                                                                   ctCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGAcc                           <  1:4012026/149‑1 (MQ=255)
                                                                                                                   aTCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGAcc                           <  1:4130498/150‑1 (MQ=255)
                                                                                                                     cGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCga                         <  1:803208/150‑1 (MQ=255)
                                                                                                                     cGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCga                         <  2:672707/150‑1 (MQ=255)
                                                                                                                     cGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCga                         <  1:1454259/150‑1 (MQ=255)
                                                                                                                     cGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCga                         <  2:1164290/150‑1 (MQ=255)
                                                                                                                           tgtTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTc                   <  2:1588068/150‑1 (MQ=255)
                                                                                                                           tgtTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTc                   >  1:2328764/1‑150 (MQ=255)
                                                                                                                                ttGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCt              >  1:2634200/1‑150 (MQ=255)
                                                                                                                                   aCGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGa           <  1:3256283/150‑1 (MQ=255)
                                                                                                                                   aCGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGa           <  1:2071563/150‑1 (MQ=255)
                                                                                                                                   aCGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGa           >  1:2130437/1‑150 (MQ=255)
                                                                                                                                   aCGCCATGAGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGATCGCGGTAAATTTACCGAAGCACAGTTTGAAAAGCTGACAGAGTGGATGTACTGGTCTCTGGCGGACCGAGATGTCGATCTGGa           >  2:3007790/1‑150 (MQ=255)
                                                                                                                                       cATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGt       <  2:4769420/150‑1 (MQ=255)
                                                                                                                                        aTGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTa      <  2:4373498/150‑1 (MQ=255)
                                                                                                                                        aTGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTa      <  1:2951376/150‑1 (MQ=255)
                                                                                                                                           cgcgAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTATCt   >  2:4565045/1‑150 (MQ=255)
                                                                                                                                            gcgAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTATCta  <  2:2265831/150‑1 (MQ=255)
                                                                                                                                            gcgAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTATCta  >  2:373443/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CCTTATTAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTATCTA  >  NC_000913/222811‑223101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: