Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 777,208 A→C 8.9% K289N (AAA→AAC)  tolA → membrane anchored protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913777,2080AC 8.9% 620.2 / 6.8 247K289N (AAA→AAC) tolAmembrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base A (140/78);  new base C (2/27);  total (142/105)
Fisher's exact test for biased strand distribution p-value = 1.83e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

AGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAA  >  NC_000913/777061‑777357
                                                                                                                                                   |                                                                                                                                                     
agagAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAACGc                                                                                                                                                     >  1:3309443/1‑150 (MQ=255)
agagAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGc                                                                                                                                                     >  1:2980814/1‑150 (MQ=255)
 gagaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCg                                                                                                                                                    >  1:1936744/1‑150 (MQ=255)
     aaGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCCGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCGGAAAAAGCCGCTGCAGCCACAGCGGCCg                                                                                                                                                >  2:5046635/1‑150 (MQ=255)
        cTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCag                                                                                                                                             <  2:3394115/150‑1 (MQ=255)
        cTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCag                                                                                                                                             >  2:4600969/1‑150 (MQ=255)
         tGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCaga                                                                                                                                            >  1:2176011/1‑150 (MQ=255)
           cAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAgg                                                                                                                                          >  1:1786438/1‑150 (MQ=255)
           cAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAgg                                                                                                                                          >  1:4517303/1‑150 (MQ=255)
               cGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAga                                                                                                                                      >  2:1097423/1‑150 (MQ=255)
                gACAAAAACGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCATAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgat                                                                                                                                     <  2:3691631/150‑1 (MQ=255)
                gACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGTCAGAAGCAGATAAGAAAGCAGCGGAAGAAAAAGCCGCCGCAGAAAAAAAAGCGGCAGCGGCAAAAGATGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCAGCAGAGGCAgat                                                                                                                                     >  2:1372795/1‑150 (MQ=255)
                   aaaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGTCGCTGCAGCAAAAGCGGCCGCAGAGGCAgatgat                                                                                                                                  >  1:3573269/1‑150 (MQ=255)
                    aaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGata                                                                                                                                 >  1:4565517/1‑150 (MQ=255)
                    aaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGata                                                                                                                                 >  1:255448/1‑150 (MQ=255)
                     aaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGatat                                                                                                                                >  2:1213085/1‑150 (MQ=255)
                     aaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGAGGCAGAAAAAGACGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGatat                                                                                                                                >  2:3578240/1‑150 (MQ=255)
                      aaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATAtt                                                                                                                               >  2:2414194/1‑150 (MQ=255)
                             cagAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTg                                                                                                                        >  2:596363/1‑150 (MQ=255)
                              agAAAACGCGGCTGCTGAAACGGCAGAAGCTTAAAAGACCGCCGCGGCAGAAACAGCCGCCGCAGACAAAAAACCGGCAGCGGAAAACGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGa                                                                                                                       <  2:3964535/150‑1 (MQ=255)
                              agAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGACAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGa                                                                                                                       <  2:2956852/150‑1 (MQ=255)
                                aaaaaGCGGCTGATGAAAAGGCAGCAGATGATAATAAAGCATCGGAAGAAAAAGACGCCGCAGACAAAAAAGCGGCAGAGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGc                                                                                                                     >  2:3169392/1‑150 (MQ=255)
                                 aacaGCGGCTGCTGAACAGGCCGCTGGTGTTAAGAAAGCAGCGGAATAAAAAGCCGCCGCACACAAAAAAGCGGCAGCGGCAAACGCTGCAGCTGAAAACGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCt                                                                                                                    <  2:915960/147‑1 (MQ=255)
                                    cgCGGCTGCTGAAATGGCAGCTGCTGATAAGACCGCAGCGGAATAAAACGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAg                                                                                                                 <  2:478895/149‑1 (MQ=255)
                                         ctgctgAAACGGCAGCAGCTGATAACAAACCAGCGGCAGAAAACGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGATGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTg                                                                                                            <  2:958138/150‑1 (MQ=255)
                                          tgctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTgg                                                                                                           <  2:1007781/150‑1 (MQ=255)
                                           gctgAAACGCCAGCCGCTCATAAGACAGCCGCGCCACAAAACGCCGCCGCAGAAAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGt                                                                                                          <  2:624876/150‑1 (MQ=255)
                                           gctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGt                                                                                                          >  1:3994405/1‑150 (MQ=255)
                                            ctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTa                                                                                                         >  2:833701/1‑150 (MQ=255)
                                            ctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTa                                                                                                         >  2:3702062/1‑150 (MQ=255)
                                             tgAACAGGCAGCAGCTGATAAGAACGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTaa                                                                                                        <  2:3860708/150‑1 (MQ=255)
                                             tgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTaa                                                                                                        <  2:2564557/150‑1 (MQ=255)
                                              gAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAg                                                                                                       >  2:5131039/1‑150 (MQ=255)
                                                aacggCAGCAGCTGATAAGAAAGCAGCGGCAGAAAACGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAACGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGaa                                                                                                     <  2:4483792/147‑1 (MQ=255)
                                                aacgccagcagCTGATAACAACCCCGCGGCACAAAACGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAACGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGaa                                                                                                     <  2:1926385/145‑1 (MQ=255)
                                                aaaGCCAGAAGCTTATAACAAACCAGCGTAAGAAAAAGCCGCCGCAGACAAAAAACCGCCAGCGTCAAAAGCTCCAGCTGAAAAACCCTCTGCACAAAAAGCGGAAGCAGAGGCAGATGATATTTTCGGTGAGATAATCTCTGGTAAGaa                                                                                                     <  2:4991766/150‑1 (MQ=255)
                                                 acggCCGCAGCTTATAATAACGCTGGGTCTGAAAACCCCGCCGAACACAAAAACGCGGCTGCGTCAAAAGCTGCAGCTGAAAAAGCCGCTGCATCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAAt                                                                                                    <  1:3358962/148‑1 (MQ=255)
                                                    gcagcaGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCa                                                                                                 >  2:3430902/1‑150 (MQ=255)
                                                      agcagcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAATCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCAcc                                                                                               >  2:4727785/1‑150 (MQ=255)
                                                      agcagcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCAcc                                                                                               <  2:795480/150‑1 (MQ=255)
                                                         agcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACCAAAAAGCGCCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGAGGATATTTTCGGTGAGCTAAGCTCTGGTAAGACTGCACCGaa                                                                                            >  2:2826916/1‑150 (MQ=255)
                                                           cttaTAAGAAAGCTGCGCAACAAAAAGCCGCCGCAGAAAAAAAAGCGGCAGCGGAAAACGCTCCAGCTTAAAAAGCCGCTGCACAAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaaaa                                                                                          <  1:761878/147‑1 (MQ=255)
                                                             gATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCCGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACg                                                                                        >  1:924408/1‑150 (MQ=255)
                                                               tAAGAACGCCGCGGCAGAAAAAGCCGCCGCAGCCAAAAACGCGGCAGCGGCAACCGCTGCAGCTGAAAACGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACggg                                                                                      <  2:4289993/150‑1 (MQ=255)
                                                               tAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACggg                                                                                      >  1:3132046/1‑150 (MQ=255)
                                                                atgAACGCAGGGGAATAAAAAGCCGCCGCAGACAAAAAAGCGGCTGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggg                                                                                     <  2:1808437/148‑1 (MQ=255)
                                                                aaGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggg                                                                                     >  2:1366679/1‑150 (MQ=255)
                                                                aaGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggg                                                                                     <  2:1711357/150‑1 (MQ=255)
                                                                  gAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggggg                                                                                   >  1:2775011/1‑150 (MQ=255)
                                                                   aaaGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGa                                                                                  >  1:481919/1‑150 (MQ=255)
                                                                   aaaGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGa                                                                                  >  2:4112555/1‑150 (MQ=255)
                                                                   aaaGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGa                                                                                  >  1:2727158/1‑150 (MQ=255)
                                                                   aaaGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGa                                                                                  >  1:1194073/1‑150 (MQ=255)
                                                                    aaGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAg                                                                                 >  2:2471910/1‑150 (MQ=255)
                                                                           ggCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCCACAGCGGCCGCAGAGGCAGCTGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCgcc                                                                          >  2:2107903/1‑148 (MQ=255)
                                                                              aGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAgg                                                                       >  1:3041827/1‑150 (MQ=255)
                                                                               gAAAAAGCAGCCTCAGAAAAAAAACCGTCAGCGGCAAAAGCTGCAGCTGAAAAAGACGCTGCAGCAAAACCGGCCGCATAGGCAGATTATATTTACGGTGAGCTAACCACTGCTAAGAATGCACCGAAAACGGGGGGATGGGCGAAAggt                                                                      >  1:4279230/1‑149 (MQ=255)
                                                                                aaaaaGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGa                                                                     >  2:3150161/1‑150 (MQ=255)
                                                                                 aaaaCCCGCCCAACAAAAAAAACCGGCAGCGGAAAAAGCTGCAGCTTAAAAAGCCGCTGCAGAAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGaa                                                                    <  2:96136/150‑1 (MQ=255)
                                                                                    aGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACaa                                                                 <  2:2459787/150‑1 (MQ=255)
                                                                                       cgccgcAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGc                                                              >  2:1579382/1‑150 (MQ=255)
                                                                                        gccgcAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCt                                                             >  2:2166883/1‑150 (MQ=255)
                                                                                        gccgcAGAAAAAAAAGCGGCAGCGCCAAACGCTGCAGCTGAAACCGCCGCTGCAGCAAAAGCGGCCGCAGCGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCt                                                             <  2:1046115/150‑1 (MQ=255)
                                                                                         ccgcAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCACAAGCGGCCGCAGAGGCAGAGGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCtt                                                            >  2:4396213/1‑150 (MQ=255)
                                                                                         ccgcAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTCAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACCATGCtt                                                            >  2:2662303/1‑150 (MQ=255)
                                                                                         ccgcAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGACCAATGCtt                                                            >  2:2662272/1‑150 (MQ=255)
                                                                                            cAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGc                                                         >  1:4210553/1‑150 (MQ=255)
                                                                                              caaaaaaatGCGGCAGCGGAAAACGCTGAAGCTTAAAACGCCGGTGCACAAAACGCGGCCGCAGAGGCATTTTATTTTTTCGGTGTGCTAAGATGTGGTATGATTGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCt                                                       <  1:700531/147‑1 (MQ=255)
                                                                                                 aaaaaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                    >  1:3089628/1‑150 (MQ=255)
                                                                                                 aaaaaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                    >  2:4232506/1‑150 (MQ=255)
                                                                                                    aaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCggg                                                 >  1:3761052/1‑150 (MQ=255)
                                                                                                     aaGCGGCAGCGGCAAACGCTGCAGCTGAAAAAGCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGa                                                <  2:4067079/150‑1 (MQ=255)
                                                                                                      agcggcagcggaAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAg                                               <  2:521153/150‑1 (MQ=255)
                                                                                                      agcgcccgcggaAAACGCGCCAGCTCAAAACCCCGCTGCACAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAATAATCCACCTAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAg                                               <  1:4664962/150‑1 (MQ=255)
                                                                                                        cggccggggcaAACGCTGCAGTTTAAAACGCCGCGGCACAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTg                                             <  2:2214800/150‑1 (MQ=255)
                                                                                                        cggcagcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTg                                             <  2:431725/150‑1 (MQ=255)
                                                                                                         ggcagcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTgg                                            >  1:12986/1‑150 (MQ=255)
                                                                                                           cagcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTa                                          >  1:3047778/1‑150 (MQ=255)
                                                                                                                gcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACt                                     <  2:4517303/150‑1 (MQ=255)
                                                                                                                gcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGACCAATGCTTCGCCTGCCGGGAGTGGTAATACt                                     >  2:3582867/1‑150 (MQ=255)
                                                                                                                gcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACt                                     >  2:2601867/1‑150 (MQ=255)
                                                                                                                   aacgctgctTCTCAAAACGCCGCTGCAGAAAACGCGGCCGCAGCGGCAGATGATATTTGGGGTGTGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaa                                  <  1:2414194/147‑1 (MQ=255)
                                                                                                                   aacgCTGCAGCTTAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaa                                  <  2:4883803/147‑1 (MQ=255)
                                                                                                                   aaaGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaa                                  >  1:500929/1‑150 (MQ=255)
                                                                                                                     cgctgctgctGAAAAAGCCGCTGCAGAAAACGCGGCCGCAGCGGCATATGATATTTTCGGTTAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaaaa                                <  1:1126834/149‑1 (MQ=255)
                                                                                                                     cgcggctgCTGAAAACCCCGCTGAAGAAAACGCGGCCGCAGTGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaaaa                                <  2:2390499/143‑1 (MQ=255)
                                                                                                                     aGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaaaa                                >  2:1585057/1‑150 (MQ=255)
                                                                                                                      gCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCAGGTAAGAATGCACCGAAAACGGGGGGAGCGGCGAAAGGGAACAATCCTTCGCCTCCCGCGAGACGAAAAACTATAAAc                               >  2:2775022/1‑150 (MQ=255)
                                                                                                                        tGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACaa                             >  1:346355/1‑150 (MQ=255)
                                                                                                                           aGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATgg                          <  2:739152/150‑1 (MQ=255)
                                                                                                                            gCTTAAAAAGCCGCTGCACAAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgc                         <  1:2280313/150‑1 (MQ=255)
                                                                                                                            gCTGACAACCCCGCGGCAGAAACCGCGGCCGCTGAGGCAGATTATATTTTCGGTGCGCTAAGCTCGGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGACCAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgc                         <  1:4416944/150‑1 (MQ=255)
                                                                                                                             cTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgcg                        >  2:2343282/1‑150 (MQ=255)
                                                                                                                               gAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTaa                                         <  2:3270352/131‑1 (MQ=255)
                                                                                                                                    aGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAgg                 >  1:3086132/1‑150 (MQ=255)
                                                                                                                                        gCTGCAGCAAACGCGGCCGCAGAGGCAGATGTTATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGcc             <  1:1463532/150‑1 (MQ=255)
                                                                                                                                        gCTGCAGCAAAAGCGGCCGCAGAGGCAGTTGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGcc             <  1:1327176/150‑1 (MQ=255)
                                                                                                                                          tGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTCAGAATGCACCGAAAACGGGGGGCGGGGCGAAAGGGCACAATGCTTCGCCGGCCGGGAGTGGTAAGACGAAAAACAATGGCGCATCAGGGGCCGa           >  1:1564351/1‑150 (MQ=255)
                                                                                                                                               caAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCa      >  1:573414/1‑150 (MQ=255)
                                                                                                                                                   aGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATaa  <  2:2028136/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAA  >  NC_000913/777061‑777357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑