Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,152 T→C 5.4% noncoding (85/171 nt)
T9A (ACT→GCT) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1520TC 5.4% 518.1 / 10.4 185noncoding (85/171 nt)
T9A (ACT→GCT) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base T (90/80);  new base C (4/6);  total (96/89)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

CCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287003‑1287298
                                                                                                                                                     |                                                                                                                                                  
    gCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGaa                                                                                                                                                <  2:1931565/150‑1 (MQ=255)
     cATGGGCAGAACATTCGCTTGCGGACGCGCTTGAGAGTTCAGCGACGTTTGAAAGTGATGGTGGTGGGGGTAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAc                                                                                                                                               <  1:1807515/150‑1 (MQ=14)
       tGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCt                                                                                                                                             <  1:2953332/150‑1 (MQ=255)
       tGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCt                                                                                                                                             <  1:2953573/150‑1 (MQ=255)
         ggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGGGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTg                                                                                                                                           >  1:916427/1‑150 (MQ=255)
          gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCGTCGGGTCGTTGCCTGAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGacaaatgg                                                                                                                                          >  2:4516219/1‑143 (MQ=255)
           cAGAATATTTGTTTGAGGATTCGCTTGAGATTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGTGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                         <  2:2865559/150‑1 (MQ=14)
           cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                         <  1:1896510/150‑1 (MQ=255)
           cAGAATATTTGATTGCGGATTCGATTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                         <  2:311410/150‑1 (MQ=255)
            aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGTTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGGCTGGGGCAAAGCTGTCTAGGTGGGGGTCGGGTGGTACCTTGGtg                                                                                                                                        >  1:1993186/1‑149 (MQ=14)
            aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCACGAGTCGAACCGTGGTc                                                                                                                                        >  2:1267716/1‑150 (MQ=255)
                   ttGATTGCGGATTCGCTTGAGATTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCtt                                                                                                                                 <  2:3456627/150‑1 (MQ=255)
                   ttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAACCTCTCAAGCGGGCGCTCGAGTCGAAACATGGTCGAACCtt                                                                                                                                 >  1:1551401/1‑150 (MQ=14)
                    tGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtc                                                                                                                                <  2:930476/150‑1 (MQ=255)
                        tGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCACACAAGCTGGCGCGCCCGTCGCACCAAGGTCGAAGcgtaacaa                                                                                                                            >  1:2270533/1‑143 (MQ=14)
                         gCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCCCGCTCTCTCGCTGGCGCTCGAGGCGAACCTTTGTCGAATCGTCTCagc                                                                                                                           >  1:523055/1‑148 (MQ=14)
                           ggATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCACTCTCGCTGGCGCTCGAGTAGAACCTTGGTCGAAGCTTCTCATCCt                                                                                                                         >  1:3816880/1‑150 (MQ=255)
                            gATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCTCTTCGCCCTTCGGGTCGTTGCCGGCGGCAAAGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCGCaaactt                                                                                                                        >  1:3420849/1‑145 (MQ=14)
                               tCGCTTGAGAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGATGCTTCTCATCCTTccc                                                                                                                     <  2:4521209/150‑1 (MQ=255)
                               tCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCACTCTCGCATGCGCTCGAGTCGAACCGTGGTCGAATCTTCTCAACCATccc                                                                                                                     >  1:1081066/1‑150 (MQ=14)
                                 gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTTCGAGCTTCGCCCTTCTGGTCGATGCCTGCCGCAACGCTCTCTCGCTGGCGCACGAGTCGAACCTTCGTCGAAGCATCTCATCCTTCCCCg                                                                                                                   >  2:2144749/1‑150 (MQ=14)
                                 gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCg                                                                                                                   >  2:2170708/1‑150 (MQ=255)
                                      agagTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                                              <  2:1890137/150‑1 (MQ=255)
                                             agtgACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAat                                                                                                       <  1:2550716/147‑1 (MQ=38)
                                              gggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCGTCTCATCCTTCCCCGCGTGGGCCGActa                                                                                                      >  2:4887090/1‑150 (MQ=14)
                                              gggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCGAGTCGAACCTTGGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGaaaa                                                                                                      >  2:955406/1‑148 (MQ=255)
                                               ggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCACTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAatat                                                                                                     >  2:4967053/1‑150 (MQ=255)
                                                 gcTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                                   >  2:3756871/2‑150 (MQ=14)
                                                   ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCGAGTCGAACCATGGTCGAAGCTTCTCAACCGTCCCCCCAGGGGGCGAATATTTaa                                                                                                 >  1:5006487/1‑150 (MQ=11)
                                                   tattGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGACTTCGGCAAAGCTCACAAGCTGGCGCACGACTAGAACCATTGTCGAATATTAAAAAACATCACCCCATGGGCAGAataataaa                                                                                                 >  1:2774808/3‑145 (MQ=2)
                                                    tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCCACGCTCTCTCGCAGGCGCTCGAGTCGAACCTTTGTCGAAACGTATCAAACTTACCCCCAGTGGCAGAAAATTTAAt                                                                                                >  1:3645407/1‑150 (MQ=11)
                                                    tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGGAGTATATTTAAt                                                                                                >  2:1099721/1‑150 (MQ=255)
                                                    atctgaaGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAt                                                                                                <  1:1406283/145‑1 (MQ=16)
                                                             gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCACACTCGCTGGCGCACGAGTCGAAACATGGTCGAAGCTTCACAAACATACCCCCAAGGGCCGAAAATTTAAATGCGGAATc                                                                                       >  2:2286251/1‑150 (MQ=11)
                                                                          gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTGGGTGGCGCTCGATTGGAACCTTTGTCGAAGCTTCTCATGCTTCCCCGCTTGGGGAGTATTTTTTATTTGGGATTGGTTGGGGAGttta                                                                          >  2:1048590/1‑148 (MQ=11)
                                                                            aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                                                                        <  1:2355917/150‑1 (MQ=255)
                                                                             ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGt                                                                                                                                                    >  2:765076/1‑73 (MQ=34)
                                                                              gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCTCTCGCTGGCGCTCGAGGCGAACCGTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGCAGAAGATTTAAATGCGGATTCGTGGGGAAGTTCAGGGa                                                                      >  2:46869/1‑150 (MQ=14)
                                                                                   cgcAGCGGTGCGCGCTGCGGCCGTCGGGTGGGTGCCTGCGGGGTGGCTGTCGGGCTGGCGGGGGAGCCGCCGCTCGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTGGGGAAGTTCAGGGACtttt                                                                 <  1:4035958/148‑1 (MQ=9)
                                                                                    tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGGGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAGCCTTCCCCGCCGGGGGCCAACATTTAATTGCGGATGCGGGTGGAAGTTCCGGGACtggtg                                                                >  2:1081197/1‑146 (MQ=14)
                                                                                    tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAatat                                                                                                     >  2:3785951/1‑113 (MQ=35)
                                                                                    tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCCGAAGGTTTAATTGCGCAGGCGGGGGGACGTTCgggggcgagtg                                                                >  2:1707519/1‑143 (MQ=11)
                                                                                      aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGACGTTCAGGGACTTTTGaa                                                              >  2:4275344/1‑150 (MQ=255)
                                                                                      aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                              >  2:3354882/1‑150 (MQ=255)
                                                                                      aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                              >  2:789680/1‑150 (MQ=255)
                                                                                      aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                              >  2:2020051/1‑150 (MQ=255)
                                                                                      aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGAATATTTAAGTGCGGATTCGTTGGGAAGTTCCGGGACGTGTGaa                                                              >  2:1297648/1‑150 (MQ=14)
                                                                                       gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                             <  1:118074/150‑1 (MQ=255)
                                                                                         gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGt                                                           <  1:2262167/150‑1 (MQ=255)
                                                                                           tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                         <  1:790011/150‑1 (MQ=255)
                                                                                           tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAAGATTTAATTGCGGGTTCGGTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                         >  2:3552601/1‑150 (MQ=14)
                                                                                           tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                         <  1:3756871/150‑1 (MQ=255)
                                                                                                   tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGTGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                                 <  1:4067649/150‑1 (MQ=255)
                                                                                                      cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                                              >  2:407469/1‑150 (MQ=255)
                                                                                                        cTTCGGGTCGTTACCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                                 >  2:3048879/1‑97 (MQ=12)
                                                                                                         ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           <  2:2270533/150‑1 (MQ=255)
                                                                                                         ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           <  2:1163289/150‑1 (MQ=255)
                                                                                                         cccGGGCCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTATTCGTTCGGAGCATCGTCTTTATATTTAACTTCGGATTCGCTGGCCAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           <  1:1146572/148‑1 (MQ=14)
                                                                                                          tCGGGTCTTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg                                          <  1:3092134/150‑1 (MQ=255)
                                                                                                            agagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCATCCCCGCATGGGCAGAATATTTAATTTCGGATTCGTTGGGAAGTTAAGGGACTTTTTAAAGTGATGGTGGTGGGGGAAGGa                                        >  1:2376168/5‑150 (MQ=17)
                                                                                                              gTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGATTCGAACCTTTGTCGAAGCTTCTCAACCTTCCCCGCATGGGCAGAAAATTTAAAATCGGAATCGTTGGGAAGTTCAGGGACTGTTGAAAGTGATGGTGGTGGGGGAAGGagt                                      >  2:507678/1‑148 (MQ=14)
                                                                                                              gTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAc                                                                                                                                               >  2:4999152/1‑45 (MQ=38)
                                                                                                               tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                                     <  2:3817217/150‑1 (MQ=255)
                                                                                                               tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                                     <  1:4281729/150‑1 (MQ=255)
                                                                                                                  ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                  <  1:46869/150‑1 (MQ=255)
                                                                                                                  ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                  >  2:4773425/1‑150 (MQ=255)
                                                                                                                  ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                  <  1:3783655/150‑1 (MQ=255)
                                                                                                                  ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           <  1:2073586/141‑1 (MQ=255)
                                                                                                                  ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           >  2:2073586/1‑141 (MQ=255)
                                                                                                                     ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCt                               >  2:2565323/1‑150 (MQ=255)
                                                                                                                      cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                             <  1:1923808/87‑1 (MQ=255)
                                                                                                                       tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCtt                                                                                                                                 >  2:4169107/1‑50 (MQ=38)
                                                                                                                       tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                             <  2:1551401/150‑1 (MQ=255)
                                                                                                                          ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                                     <  1:4884790/139‑1 (MQ=255)
                                                                                                                          ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa                          <  1:2326775/150‑1 (MQ=255)
                                                                                                                           gCAACGCTCTCTCGGTGGCGCTCGAGTCGAACCTTGGTCGATGCTTCTAAGCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAg                         <  2:540647/150‑1 (MQ=17)
                                                                                                                           gCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAg                         <  2:1476640/150‑1 (MQ=255)
                                                                                                                            cAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCTCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGt                        <  1:2105528/150‑1 (MQ=255)
                                                                                                                            cAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGt                        >  2:2647287/1‑150 (MQ=255)
                                                                                                                             aaCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTc                       <  1:3041771/150‑1 (MQ=255)
                                                                                                                               cgcgCTCTCGCGGGCGCGCGAGCCGCCCCGTGGGCGTAGCTTCTCAGCCGTCGCCGCATGGGCTGTATATTTTTGTGCGGAGTCGTTGGGTTGTGCCGGGCGTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGa                     <  1:3510636/150‑1 (MQ=12)
                                                                                                                               cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGa                     <  2:874350/150‑1 (MQ=255)
                                                                                                                                gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCg                                                                                                                                       <  1:4399227/35‑1 (MQ=40)
                                                                                                                                gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                         >  1:1967047/1‑113 (MQ=255)
                                                                                                                                gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                         <  2:1967047/113‑1 (MQ=255)
                                                                                                                                gCTCTCTAGCTGGCTCTCGAGTCGAACCTTGGTCGAAGCTTCTGATCCGTGCCCGCATGTGTAGTATTCTTATCTGCGGATTCGTTTGTTAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                    <  1:4568760/150‑1 (MQ=17)
                                                                                                                                  tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                  >  2:1400888/1‑150 (MQ=255)
                                                                                                                                  tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGCAGAAGATTGAAATGCGGAGTCGTGGGGCAGGACAGGGACTGTGGAAAGTGGTGGTCGTGGGGGAGGGATTCGAACCTTCGAAGTCGATgg                  >  2:1847648/1‑149 (MQ=18)
                                                                                                                                    tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACg                >  2:2935358/1‑150 (MQ=255)
                                                                                                                                     ctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCCCAAGGGCAGAATATATAATTTCGGATTCGTTGGGAAGTTAAGGGACATTTGAAAGTGATTGTGGTGGGGGAAGGATTCGAACCATCGAAGTAGATGAAgg               >  1:4193786/1‑150 (MQ=18)
                                                                                                                                        gCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAg            <  1:3104731/150‑1 (MQ=255)
                                                                                                                                        gCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTAGAAGTCGATGACGGCAg            >  1:1227674/1‑150 (MQ=255)
                                                                                                                                              gcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGGTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAGAACCTTCTAAGACGATCACCGCAGATTTAc      >  1:693819/1‑150 (MQ=14)
                                                                                                                                               cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa     <  1:4168382/150‑1 (MQ=255)
                                                                                                                                               cTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGCAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa     <  1:4822544/150‑1 (MQ=255)
                                                                                                                                                tcaaGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg                                                                                            <  1:269857/59‑1 (MQ=25)
                                                                                                                                                tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAtt                                                                                                    <  2:4742043/54‑1 (MQ=255)
                                                                                                                                                tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAg    >  2:4446732/1‑150 (MQ=255)
                                                                                                                                                 cGAGTCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGt   <  2:2408428/150‑1 (MQ=255)
                                                                                                                                                  gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc  <  1:1340170/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287003‑1287298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: