Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 164,581 | A→C | 20.7% | intergenic (+47/‑149) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 164,581 | 0 | A | C | 20.7% | 192.1 / 15.3 | 123 | intergenic (+47/‑149) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Reads supporting (aligned to +/- strand): ref base A (32/63); new base C (6/22); total (38/85) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.52e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 125 total aligned reads displayed.
CCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA > NC_000913/164432‑164730 | cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt > 1:451544/1‑150 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt > 2:1200040/1‑150 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt > 2:3088161/1‑150 (MQ=255) gTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATc > 1:480864/1‑150 (MQ=255) cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGTCGGGGTGAGGGCATCAGCGCGCa > 2:4836622/1‑150 (MQ=255) aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCAc > 2:3332490/1‑150 (MQ=255) aaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACg > 2:874066/1‑150 (MQ=255) aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGt > 2:4038042/1‑150 (MQ=255) cgaTGAACGGGCGTTATCCCAACCATGTCTGGCCGGACGCCCCGGCAAATACGGCACCGACGCGACGGACGAAAAAGTTTGCTTAAGGTTGTTTTTCTCCCTCTCCCTGTGGTTGAGGGCCGGGTTGAGGGCATCAGCGCGCACGTtcac < 2:4649209/149‑1 (MQ=255) taTGAACGGGCGTTACCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGCCGGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacc < 2:4954851/149‑1 (MQ=38) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccc > 1:4637082/1‑150 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTtcaccctc > 1:3843570/1‑150 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctc > 1:4512819/1‑150 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacccac > 1:4283554/1‑148 (MQ=255) aaGGGCGTTACCCCAACCAGGCCGGGCCGGACGACCCGGCAAATCCTGCCCCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCGCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcaccctcac < 2:1174453/150‑1 (MQ=37) gggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcaccctcaccc < 2:1187937/150‑1 (MQ=255) gggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGAACCGACGCGACGGAGGAAAATGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcaccc < 2:1187996/150‑1 (MQ=38) ggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcacccacacccc > 2:3030252/1‑149 (MQ=255) cGTTATCCCAACCATGTCTGGCCGGACGACCCGGCAAACACTGCACCGACGCGCCGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTaa < 2:3288066/150‑1 (MQ=255) gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGGTTTTCTCCCTCGCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAc < 2:1975511/150‑1 (MQ=255) gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGCCCCACACCCAAAc > 2:3993118/1‑150 (MQ=38) gTTATCCCAAACATGTCGGGCCGGACGACCCGGAAAATACGGCACCGACGCGACGGACTAAAAAGTATTCTTAAGTTTGGTTTGCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAc < 2:711541/150‑1 (MQ=38) gTTAGCCCAACCAGGTCGGGCCGGACGACCCGGCAAATCCTGCACCGACGCGCCGGACGAAAAAGTATTCGTAAGGGTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAc < 2:711507/150‑1 (MQ=37) ttttCCCAAACTTTTGTGGCCGGACGACCCGGCAAAGACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:834657/147‑1 (MQ=38) ttttCCCAAACTTTTCTGGCCGGACGACCCGGAAAATACGGCACCGCCGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCGTGTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:834817/147‑1 (MQ=38) ttATCCCACAGCTGTGGGGCCGGACGCCCCGGCAAATACTGCACCGACGCGCCGGAGGAAAATGTTTGGGTATGGTTGGTTTTCGCCCTCGCCGGGGGGGTGAGGGCGGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:4534264/150‑1 (MQ=255) ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGCCGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:834651/150‑1 (MQ=255) ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCAGGTTCACCCGCACCCGAAcc > 2:3269308/1‑150 (MQ=39) ttATCCCAAACATGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGTTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:1237082/150‑1 (MQ=255) cccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATGCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGCGCCCGCACCCAAGcccaca > 1:3248782/1‑147 (MQ=37) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGCACCCGCCCCCTAAaccaaaa > 1:2030030/1‑146 (MQ=38) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCAACCAAAcccacac > 1:3695009/1‑146 (MQ=38) aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCGCACCCCAACCCCCGccc > 2:4890369/1‑150 (MQ=38) aaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGGGCCCCCCCCCCCCAACCCCCAcccc > 1:4156677/1‑149 (MQ=37) atttgtGGCCGGACGACCCGGAAAATACTGCACCTACGCGACGTACTAAAATTTATTCTTATGGTGGGTTTTCTCCCTCCCCGTGTGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:1103105/145‑1 (MQ=255) aggTCGGGCCGGACGACCCGGCAAATCCTCCCCCGACGCGCCGGACTAAACCGTATTCGTATGGTGGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:3300421/148‑1 (MQ=25) aTGTCTGGCGGTACGCCCCGGCAAATACTGCACCGAGGCGACGGACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:1699713/150‑1 (MQ=38) tgacTGGCCGCACGACCCGGAAAATACTCCACCGACGCGACGCACCAAAAACTATACGTAAGGTTGTTTTTCTCCCTCACCCCGTGGGAGAGGCCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:2545958/147‑1 (MQ=37) tGTCTGGCCGGACGACCCGGCAAATCCGGCACCCCCGCGCCGGACCAAAATTTTTTCGAACGGTTGGTTTTCCCCCTCCCCCGTTGGGAGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:3506668/150‑1 (MQ=255) gTCGGGCCGGACCACCCGCCAAACACGCCCCCGCCGCGCCGGCCGAAAACGTTTTCTTATGGTTTGTTTTCCCCCTCGCCGTGTGGGAGAGGGCCGGGGTTTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg < 2:3034367/150‑1 (MQ=255) ccGCACGACCCGCCAAATCCGGCACCGACGCGACGGACGAAAATTTATTCTTACGGTTGGTTTGCCCCCCCCCCCGGTGGGAGTGGGCCGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 2:2399012/150‑1 (MQ=255) ggACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGTGGCCCCACACCCGAACCCACCCCCCCACgggggggggg > 2:4931599/1‑150 (MQ=25) ggACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACACCCGAAAACACTCCCACAAaggcgggtgg > 2:4931555/1‑143 (MQ=25) gacgccCCGGCAAATACGGCACCGACGCGACGGACGACAAAGTATTCGTAAGGTTGGTTTTCTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCCACAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 2:3351156/150‑1 (MQ=39) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 2:5081377/150‑1 (MQ=255) gacgacCCGGCAAACACGCCCCCCCCGCGACGTACGAAAATGTATTCTTATGGTGGGTTTTCACCCCCTCCCTGTGGTTGCGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:4932994/150‑1 (MQ=255) ccccccccGGAAAATACTGCACCGCCCCGACGGACTAAAAATTATTCTTACGGTTGTTTTTCTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:3355653/145‑1 (MQ=255) acgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCACCCCGACCCGCGCCCCCACGGGGGAgggggg > 1:989918/1‑148 (MQ=25) gacCCGGCAAATACTGCACCGACGCGACGTACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTTAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCg < 2:2906863/150‑1 (MQ=255) gacCCGCAAAACACTGCACCGCCGCGCCGGAAGAAAATGTTTTCGTATGGTTGTTTTCCTCCCTCTCCGTGTGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCg < 1:3214391/150‑1 (MQ=255) ccGCCAAATACGGCACCGACGCGCCGGACTAAAAAGTATTCGTAAGGTTGGTTTCCTCCCACTCCCTGTGGGAGAGGGCGGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc < 2:122378/150‑1 (MQ=255) cGGCAAATACTGCACCGACGCGACGGACTAAAATTTTTTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCg < 2:4501213/150‑1 (MQ=38) cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCACAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCCCACCCAAACCCTCGCCCGCAAGGGCGAGGGGGCCgcgcg > 1:4236200/1‑146 (MQ=37) gtaaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCACACCCAAACCCACACCCACCACGGGGAGGGGGCCCATCGac > 2:1907412/3‑149 (MQ=25) aCTGCACCTACGCGACGGACTAAAAAGTATTCGTAAGTTTGGTTTTCTCCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACaaa < 2:3760663/150‑1 (MQ=255) cACCGCGGCGCCGGACTAAAATTTATTCGTTTGGTTGTTTTACCCCCACTCCGTGGGGGAGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:2555015/150‑1 (MQ=255) cgtacgcgactGACTAAAATTTATTCTTATGGTTGTTTTTCTCCCTCTCCCTTTGGTTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:796438/147‑1 (MQ=255) ccgccgCGCCGGCCGAAACTGTATGCGTATGGTGGGATTCCGCCCACTCCCTGTGGGCGAGGGCCGGGGTGTGCGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGTGGACCGATCGAGCACAAATTTTga < 2:3091221/150‑1 (MQ=255) ccgccgCGACGGACTAAAAAGTATTGGTACGGTTGTTTTTCTCCCTCTCCCGGGGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:3685889/150‑1 (MQ=38) ccGACGCGACTTACTAAATTTTATTGGTTTGTTTGGTTGTCGCCCTCCCCGTGTGGTTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:2023905/150‑1 (MQ=255) ccGACGCGACGGACGAAAATGTATTGGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:2591858/150‑1 (MQ=255) ccGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:2734210/150‑1 (MQ=255) gccgcgacgGCCGAAAATGTTTTGGTATGGTTGTTTTGCCCCCTCGCCGGGGGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga < 2:525303/148‑1 (MQ=255) gacgcgacgGACGACCACGTATTCGTATGGTTGGTTTTCCCCCACTCCCTGTGGGTGCGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga < 2:2712852/150‑1 (MQ=255) gacgcgacgGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga < 2:2713088/150‑1 (MQ=255) gacgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGTGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga < 2:4250193/150‑1 (MQ=255) acggactAAAATTTATTCTTTTGTTTGGTTTTCTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTATCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc < 1:2985512/150‑1 (MQ=37) gacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttc < 2:4700384/150‑1 (MQ=255) cgAAAAAGTTTTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt < 2:2669266/150‑1 (MQ=255) cgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCCAACCAACTCCCACAAGGGTGAGGGGAGCGATAGAGCCCAAATATTTGGAGATAtattaat > 2:3232253/1‑147 (MQ=25) gAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCAAAACCACACCCACAAAGGAGAGGGGACCGAACGAGCACAAAATTTGATAGAAAtattaatc > 2:3410738/1‑146 (MQ=25) aaaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttct < 2:5104183/150‑1 (MQ=255) gTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 1:3823951/150‑1 (MQ=255) gTATTCGTAAGGTTGGTTTTCTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 1:3824204/150‑1 (MQ=255) gTATTCGTAAGGTGGGTTTTCCCCCTCGCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:4199474/150‑1 (MQ=255) tttCGTACGGTTGTTTTTCTCCCTCTCCGTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt < 1:5139795/149‑1 (MQ=39) gTATGGTTGGTTGCCTCCCTCGCCGTGTGGGTGCGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGACTTGTAACa < 1:2538153/150‑1 (MQ=255) aGGTGGGTTTCCCCCCTCTCCCTGTGGGTGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 2:4970512/150‑1 (MQ=255) gtttggttTTCTCCCTCTCCCTGTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaag < 1:2938907/148‑1 (MQ=255) gttggttTTCTCCCTCTCCCTGTGGGAGAGGGTGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga < 1:4836622/150‑1 (MQ=255) gtttttCCCCCTCCCCGGGTGGGTGTGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa < 1:3935136/148‑1 (MQ=255) ccggccctctcccaCAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCCAACCCGCTCCCGCAAGGGAGTGGGGGCCGATCGAGCCCAAAATTTGGGAGATATATTATTATGTCTTGTAACAGGCGAACAGaaa > 2:823657/5‑150 (MQ=255) tccctctccctGTGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 1:929950/150‑1 (MQ=255) ccctctccctGGGGGCGTGGGCCGGGGTGAGGGCATCCGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAGCg < 2:4555377/150‑1 (MQ=255) cccacgccctGTGGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg < 2:1242200/144‑1 (MQ=255) ccctGTGGTTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt < 1:1854698/150‑1 (MQ=255) gggggTGAGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcg < 1:1945056/148‑1 (MQ=255) ggtgagGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 1:448302/147‑1 (MQ=255) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTg < 2:3843570/150‑1 (MQ=255) agagGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCACAACCGAACCCACACCCGCCACGGCGCGGGGACCGCACGCGCACAAATATTGAGAGATATAATCTTCTGTCGTGTTACACAAGTCCAGGAAAAAGGGCGTTTGCGccggt > 2:2134344/1‑147 (MQ=255) ggCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCCCACCCAAAACCACACCCACACGGGGGAGGGGCCCCAACGAGCACAAAAATTGAGAGGTATCATATTATGTCTTGTAACAGAAGAAAAGAAAAAACGGCTTTTTCGCCGGAaaat > 2:597083/1‑150 (MQ=255) gCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATAtt < 2:2135134/150‑1 (MQ=255) ggggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:1365235/150‑1 (MQ=255) ggggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:2944150/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:1141669/150‑1 (MQ=255) gggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 2:5094941/150‑1 (MQ=255) ggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 1:1200040/150‑1 (MQ=255) gccATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 2:488085/148‑1 (MQ=255) cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGc < 1:128893/150‑1 (MQ=255) aTCAGCGCGCACGTTCACCCGAAACCCAACACACTCCCACAAGGGAGAGGGGACCGCTAGAGCACAAATTTTGATAGATATTTTATTCTGTATTTTAACAGAAGAACAGCAAAAACGGCTTTTGCGCCAGAAAAATGCGGAGAAAAAGCa > 2:3868344/1‑150 (MQ=255) | CCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA > NC_000913/164432‑164730 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |