BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,818,049	T→A	8.5%	intergenic (‑189/+10)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,049	0	T	A	8.5%	703.7 / 38.3	214	intergenic (‑189/+10)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (105/90); new base A (12/7); total (117/97)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

ACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGA  >  NC_000913/2817900‑2818197

actaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGACTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAttt                                                                                                                                                      >  1:2641816/1‑150 (MQ=255)

  taAAGCCATATCTATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                    <  2:798222/150‑1 (MQ=255)

   aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCgg                                                                                                                                                   <  1:732799/150‑1 (MQ=255)

    aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                                  >  1:4857747/1‑150 (MQ=255)

       ccATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa                                                                                                                                               >  2:105398/1‑150 (MQ=255)

       ccATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa                                                                                                                                               >  2:4909936/1‑150 (MQ=255)

       ccATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa                                                                                                                                               >  2:4716581/1‑150 (MQ=255)

       ccATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa                                                                                                                                               >  2:4410961/1‑150 (MQ=255)

          tatCAATTAAGAGATGGTGCATCCGGGAGGATTACACGCCGAGCGGCTCGCCCTTCGGGCCGTTGCAAAAGCACCGTTATCATCCCTAGAGCCAGCGGTTAATTCAGAGACCTCTTCGGTACCTTCATTGCGGAAGATGTCGGAGAatct                                                                                                                                            >  1:229888/1‑148 (MQ=17)

           atCAATTAAGAGCTGGTGCATCCGGGAGGTGTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTATAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg                                                                                                                                           <  1:3491526/150‑1 (MQ=18)

           atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg                                                                                                                                           >  1:3440782/1‑150 (MQ=255)

           atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg                                                                                                                                           <  1:1654214/150‑1 (MQ=255)

            tCAATTAAGAGGTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGATATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg                                                                                                                                          >  2:1750174/1‑150 (MQ=255)

             cAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGt                                                                                                                                         <  1:284535/150‑1 (MQ=255)

              aaTTAAGAGATGGTGCATCCGGGAGGTGTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTg                                                                                                                                        <  1:3327898/150‑1 (MQ=255)

                   agagATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATcc                                                                                                                                   >  1:4350515/1‑150 (MQ=255)

                   agagATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCATTGCTAAAGCAACGTTATCATCCGTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGATTATGGTGCATcc                                                                                                                                   >  2:3700869/1‑150 (MQ=255)

                      gaTGGTGCATCCGGGAGGATTACTCGCCTAGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTAGATACCATCATTGCTGAAGATATCGGAGAATATGGTGCATCCggg                                                                                                                                >  1:4540892/1‑150 (MQ=255)

                       aTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGgga                                                                                                                               >  1:4084796/1‑150 (MQ=255)

                       aTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGgga                                                                                                                               <  2:3631686/150‑1 (MQ=255)

                          gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAATCAACGTTATAATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTAGGAGAATATGGTGCATCCGggagga                                                                                                                            >  1:917679/1‑150 (MQ=255)

                           tGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAt                                                                                                                           <  1:5097001/150‑1 (MQ=255)

                            gCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAtt                                                                                                                          >  1:4867999/1‑150 (MQ=255)

                            gCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAtt                                                                                                                          >  2:1023484/1‑150 (MQ=255)

                             cATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTc                                                                                                                         >  2:4029843/1‑150 (MQ=255)

                              acccGGGAGGACCACTCGCCTTGCGGCTCGCCCTACGGGCCGTTGCTAAAGCAACGTTCGCCTCCCTAGTGCCATCGGTTAATTCCGCTACCTCTTCGATACCCGCCTCGCTGAAGATCTCGGAGAATATGGTGCATCCGGGAGGATTCg                                                                                                                        <  2:2273671/148‑1 (MQ=17)

                                 cGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAc                                                                                                                     <  2:4867420/150‑1 (MQ=255)

                                 cGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAc                                                                                                                     <  2:3401782/150‑1 (MQ=255)

                                   ggaggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCt                                                                                                                   >  2:2093619/1‑150 (MQ=255)

                                   ggaggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCt                                                                                                                   <  2:869437/150‑1 (MQ=255)

                                     aggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTcc                                                                                                                 <  2:3446680/150‑1 (MQ=255)

                                     aggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTcc                                                                                                                 >  1:1692207/1‑150 (MQ=255)

                                       gcttACCCGCCACGCGGCTCGCCCTCCGGGCCGTTGCTAAAGCACCGTTATCAGCCCTAGGGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAAGATGGTGCATCCGGGAGGATTCGAACCTCCGa                                                                                                               <  1:4008909/148‑1 (MQ=18)

                                       gaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGa                                                                                                               >  1:3949826/1‑150 (MQ=255)

                                        aTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGAc                                                                                                              <  1:1382541/150‑1 (MQ=255)

                                            cTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                          >  1:502965/1‑150 (MQ=255)

                                             tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                                                         >  2:3774343/1‑150 (MQ=255)

                                              cGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCg                                                                                                        <  1:1425562/150‑1 (MQ=255)

                                              cGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAATCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCg                                                                                                        >  1:735703/1‑150 (MQ=255)

                                               gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCgg                                                                                                       >  2:2029196/1‑150 (MQ=255)

                                                      ggCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg                                                                                                >  2:5112082/1‑150 (MQ=255)

                                                          cGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCAGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                                            >  1:1165021/1‑150 (MQ=255)

                                                           gCCCTTCGGGCCGTTTCTAAAGCAACCTTATCATCCCTAGTGCCAGCGGTTAATTCCGCTACCACTTCGATACCAAAATTGCTGAAGATGTAGGAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTTGCCggg                                                                                           >  1:4845109/1‑148 (MQ=17)

                                                            cccTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGGTCGAAGCCGAGt                                                                                          >  2:1894490/1‑150 (MQ=255)

                                                             ccTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa                                                                                         >  2:3952368/1‑150 (MQ=255)

                                                                 cGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActct                                                                                     <  2:4965617/150‑1 (MQ=255)

                                                                   taacGTTGCTTTAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAt                                                                                   >  1:1790119/4‑150 (MQ=255)

                                                                    gCCGTTGCTAAAGCAACGTTATCATACCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                                                  >  2:1281112/1‑150 (MQ=255)

                                                                         tGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                             <  2:2641816/150‑1 (MQ=255)

                                                                           cTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                                           <  1:3427757/150‑1 (MQ=255)

                                                                            tAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                          >  1:869891/1‑150 (MQ=255)

                                                                               aGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACATCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                                                                       >  1:369077/1‑150 (MQ=255)

                                                                                gCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAc                                                                      >  1:4307026/1‑150 (MQ=255)

                                                                                 cAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                                                     >  1:744810/1‑150 (MQ=255)

                                                                                     gTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                                 >  1:4792195/1‑150 (MQ=255)

                                                                                     gTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                                 >  1:3629788/1‑150 (MQ=255)

                                                                                     gTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCACTTCGATACCTTCATTGCTGAAGATTTCGCAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGCTTCGTAGCCGAGTACTCTATCCAGCAGAGCTACGGAtc                                                                 >  1:3089638/1‑149 (MQ=255)

                                                                                     gTTATCATCCCTAGTGCCTGCGGTTAATTCCGCAACCTCTTCGATACCTTCATAGCTGAAGATGAAGGAGAAAATGTAGCATCCGGGAGGATTCGAAACACCGACCTCTCGGTACGTAGCCGAGTACTCAATCCAGCACAGCTACGGATg                                                                 >  2:3430174/1‑150 (MQ=255)

                                                                                      ttATCCTTCCTTGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGc                                                                <  1:1291307/150‑1 (MQ=255)

                                                                                        atcatcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAt                                                              >  2:2617740/1‑150 (MQ=255)

                                                                                         tcatcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATc                                                             >  1:2445221/1‑150 (MQ=255)

                                                                                          catcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                            <  1:225356/150‑1 (MQ=255)

                                                                                              ccTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaa                                                        >  2:273387/1‑150 (MQ=255)

                                                                                                 aGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatt                                                     <  2:4084796/150‑1 (MQ=255)

                                                                                                 aGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatt                                                     <  2:1676956/150‑1 (MQ=255)

                                                                                                  gTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAattt                                                    <  2:4350515/150‑1 (MQ=255)

                                                                                                   tGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAactta                                                   <  1:1326037/150‑1 (MQ=255)

                                                                                                   tGCCTGCGGTTAATACCGCTACCTCTTCGATACCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAattta                                                   <  1:1602332/150‑1 (MQ=25)

                                                                                                    gCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttat                                                  <  1:2339946/150‑1 (MQ=255)

                                                                                                        tcGGTTAATTCCGCGACCTCTTCGATACCTTCCTTGCTGCAGATGTCGGAGCATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTa                                              <  2:2403960/149‑1 (MQ=14)

                                                                                                            ttAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCCGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgc                                          >  2:151870/1‑150 (MQ=255)

                                                                                                            ttAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgc                                          <  1:651843/150‑1 (MQ=255)

                                                                                                               ataccGCTATCTCTTCGATACCTTCATTGCTGAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                       <  1:1970663/147‑1 (MQ=16)

                                                                                                               aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                       <  2:735703/150‑1 (MQ=255)

                                                                                                                     cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGa                                 <  2:736155/150‑1 (MQ=255)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                            >  2:4468794/1‑150 (MQ=255)

                                                                                                                           ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGc                           >  2:4235210/1‑150 (MQ=255)

                                                                                                                            ttCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                       <  2:1376277/137‑1 (MQ=18)

                                                                                                                            ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTg                                                                                                                                        <  2:5054041/40‑1 (MQ=255)

                                                                                                                              cGATTCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGctac                        <  1:558499/150‑1 (MQ=255)

                                                                                                                              cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGctac                        <  1:1023484/150‑1 (MQ=255)

                                                                                                                                aTACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTa                                              <  1:2002162/126‑1 (MQ=17)

                                                                                                                                  aCCTTCAGTTCTGAAGTTTTCTGTGATTATTGTGGTTCGGGGATGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCACCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa                    <  1:1627311/150‑1 (MQ=14)

                                                                                                                                   ccTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAg                   <  2:2046411/150‑1 (MQ=25)

                                                                                                                                    cTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCGGATATTGATACCGCTACTAAAGc                  >  1:3527213/1‑150 (MQ=16)

                                                                                                                                     ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGcc                 >  1:663132/1‑150 (MQ=18)

                                                                                                                                     ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTCCTGCTGATATTGATACAGCTACTAAAGcc                 >  1:2693645/1‑150 (MQ=255)

                                                                                                                                     ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGcc                 >  2:3479506/1‑150 (MQ=255)

                                                                                                                                     ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTACTGCTGATATTGATAAAGCTACTAAAgac                 >  2:4817028/1‑148 (MQ=255)

                                                                                                                                      tCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCa                >  1:107801/1‑150 (MQ=18)

                                                                                                                                      tCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCa                <  1:1367588/150‑1 (MQ=255)

                                                                                                                                        aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                    <  1:4471991/64‑1 (MQ=40)

                                                                                                                                        aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCata              >  2:4777878/1‑150 (MQ=255)

                                                                                                                                          tGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                                           >  2:1141481/1‑87 (MQ=38)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg         <  2:2872756/150‑1 (MQ=255)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg         <  2:2837374/150‑1 (MQ=255)

                                                                                                                                              gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTATTTTACTGCTGATATTTATACCGCTACTAAAGCCATATCAAGt        >  2:3315087/1‑150 (MQ=255)

                                                                                                                                                    ttCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGt                                                                                                  >  2:3626309/1‑54 (MQ=33)

                                                                                                                                                    ttCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGAACGCTCGGTTCGTAGCCGAGTACTCTATCAAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGTTACCGCTACTAAAGCCATGTCTAGTAagagg  >  1:4316951/1‑149 (MQ=14)

                                                                                                                                                    ttCGGAGAATATGGTGCATCCGGGAGGATTCGAAACTCCGACAGCACGGTTAGTAGACGAGTAAAATATCCAGATGAGCTAAGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAGgaga  >  1:1758324/1‑150 (MQ=14)

ACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGA  >  NC_000913/2817900‑2818197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`