Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,867 T→A 14.8% intergenic (+84/+68) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8670TA14.8% 543.0 / 13.1 223intergenic (+84/+68)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (39/137);  new base A (35/2);  total (84/139)
Fisher's exact test for biased strand distribution p-value = 4.13e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

CACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAA  >  NC_000913/3504718‑3505016
                                                                                                                                                     |                                                                                                                                                      
cACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCt                                                                                                                                                        >  1:2573893/1‑150 (MQ=255)
   gTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGTTGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATACCGTACGTTACCCACACCCAAAc                                                                                                                                                    >  2:2297848/1‑150 (MQ=39)
   gTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGACTGTTACATCCCGTACGGTCCCCACCCCCCAAc                                                                                                                                                    >  1:4027497/1‑150 (MQ=38)
   gTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGAATTTTACATCCCGTACGTTCCCCTCACCCaaaa                                                                                                                                                    >  1:4564865/1‑149 (MQ=255)
      tcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                                 <  2:3723663/150‑1 (MQ=255)
      tcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAacca                                                                                                                                                 >  1:2122812/1‑149 (MQ=255)
       ccGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTTCATCCCGTACGTTGCCCTCACCCTAAaccac                                                                                                                                                >  1:4691175/1‑148 (MQ=255)
        cGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCctct                                                                                                                                               >  1:4625173/1‑150 (MQ=255)
        cGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCCACCCAAAcccaca                                                                                                                                               >  1:1881717/1‑147 (MQ=255)
         gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCtctc                                                                                                                                              <  2:5071866/150‑1 (MQ=255)
         gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCtctc                                                                                                                                              <  2:4431197/150‑1 (MQ=255)
          aaCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTcc                                                                                                                                             <  2:173994/150‑1 (MQ=255)
               ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                        <  1:3343303/150‑1 (MQ=255)
               ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                        <  2:2116045/150‑1 (MQ=255)
               ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                        <  2:885955/150‑1 (MQ=255)
               ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCTCTCCCCaaa                                                                                                                                        >  1:5126450/1‑150 (MQ=255)
               ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCcaa                                                                                                                                        >  2:352605/1‑150 (MQ=255)
                tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                                       <  1:2249680/150‑1 (MQ=255)
                 ctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGATATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCTCTCCCCAAAgg                                                                                                                                      >  2:1699164/1‑150 (MQ=255)
                  tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAggg                                                                                                                                     <  1:3865421/150‑1 (MQ=255)
                  tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAggg                                                                                                                                     <  2:1814374/150‑1 (MQ=255)
                   gCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgggg                                                                                                                                    <  1:3685961/150‑1 (MQ=255)
                   gCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCAACTACCCACAgggg                                                                                                                                    >  1:2293927/1‑150 (MQ=39)
                    ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGCATAAGATGCGCCAGCAACGCAGCCGGCGATGCTGGCGCGTAGAATTTTACATCCCGTACGATCACCTCACCCAAACACTCACCCCAAAGGGGc                                                                                                                                   >  1:3764822/1‑150 (MQ=255)
                      gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                                 <  2:1659321/150‑1 (MQ=255)
                      gcgcCAATCGCATCACCGGCCCTAGTGAGAATAAAAGACCGGCACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTCACCCTCTCCCCCCCGGGGCGa                                                                                                                                 <  2:1252847/150‑1 (MQ=25)
                        gcCAATCGCATCACCTTAACTATTGATAATAAAAGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgg                                                                                                                               <  2:3675685/150‑1 (MQ=39)
                         ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                              <  2:59571/150‑1 (MQ=255)
                         ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAggg                                                                                                                              <  1:3701869/150‑1 (MQ=39)
                          cAATCGCATCACCTTAACAATTGATAATAAAAGACATTAACAGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCACACCCCAAAGGGGCGAgggg                                                                                                                             >  2:2864007/1‑150 (MQ=39)
                           aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCGAACCCACAACCCCAAAGGGCGCggggg                                                                                                                            >  1:1876898/1‑149 (MQ=38)
                           aaTCGCATCACCTTAAATATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGTTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                            <  1:1658293/150‑1 (MQ=255)
                            aTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAgggtta                                                                                                                           <  2:3154067/150‑4 (MQ=255)
                             tCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAcc                                                                                                                          <  2:2745484/150‑1 (MQ=255)
                                cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCGAACCCACACCCCCAAGGGGCGAGGGGACCGtt                                                                                                                       >  1:4983826/1‑150 (MQ=39)
                                 aTCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCGAACCCACAACCCCAAGGGGCGAGGGGACCGTTc                                                                                                                      >  1:1153671/1‑150 (MQ=38)
                                  tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCa                                                                                                                     <  2:3481659/150‑1 (MQ=255)
                                      cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                                 <  2:4585854/150‑1 (MQ=255)
                                        tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCCAAAGGGCCAGGGGACCGTGCAATACgg                                                                                                               >  1:1112763/1‑150 (MQ=38)
                                        tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCACACCCCAACCCACAACCCAAAGGGGCGAGGGGGCCGGTCAGTAccg                                                                                                               >  1:2625768/1‑148 (MQ=38)
                                          aCTATGGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                                             <  1:4587362/150‑1 (MQ=255)
                                            tATTGATAATAAAAGACATTAACCGGAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCCCCCGAACCCACACCCCCCAGGGGCGCGGGGCCCGGTCAGTACggtctt                                                                                                           >  2:4078573/1‑146 (MQ=37)
                                              ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACAACCCCAAGGGGCGAGGGGCCCGGTCAGTACGGAAATTa                                                                                                         >  1:1945990/1‑150 (MQ=38)
                                                 ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                                      <  1:48657/150‑1 (MQ=255)
                                                  taataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCAACCAAAACCACAACCCAAAGGGGCGAGGGGACCGGTCAGTAAGGAAATTatcat                                                                                                     >  1:2738933/1‑146 (MQ=37)
                                                      aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGAATTTTACATCCCGTACGTTGCCCGCACCCAAAACCACACCCCCAAGGGGCGGGGGGGCCCGTGAGTGAGGAAGTTAGCGTGTCg                                                                                                 >  1:973418/1‑150 (MQ=21)
                                                       aaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCTCAACCCAAAGGGGCGGGGGGACCGTTCAGTACGGAAATTAGCTTGACgg                                                                                                >  2:673196/1‑150 (MQ=39)
                                                       aaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCTCTCCCCAAACGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACgg                                                                                                >  2:4141818/1‑150 (MQ=255)
                                                       aaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAACCAAAACCACAACCCCAAGGGGCGAGGGGCCCCTTCAGTAACGAAATTAGcattaagg                                                                                                >  1:2795873/1‑143 (MQ=25)
                                                       aaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTAAACCAAAACCAATACCCAAAGGGGCCAGGGGACCGGTCAGAACGGAAATTAGCtataccg                                                                                                >  2:673500/1‑144 (MQ=25)
                                                         aGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                              <  2:2363858/150‑1 (MQ=255)
                                                          gACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                              <  2:299416/150‑1 (MQ=255)
                                                          gACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGc                                                                                             <  2:2057266/150‑1 (MQ=255)
                                                          gACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGAATTTTACATCCCGTACGGTCCCCACACCCAAACCCACACCCCCAACGGGCCCGGGGCCCGGTCAGTAAGGCAAGTAGCTTGACGGTGc                                                                                             >  1:3392928/1‑150 (MQ=25)
                                                               tAACCGTCGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTTCGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATcc                                                                                        <  1:716191/150‑1 (MQ=255)
                                                                  ccGTTGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGc                                                                                     <  1:1308698/150‑1 (MQ=255)
                                                                     tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGTATTTTAAATCCCGTACGGTACCCCCACCCGAAACCAAAACCCAAAGGGGGGAGGGGGCCGGTGCGGACGGAAATTTTCTTGTAGGGGCTATCCAGccat                                                                                  >  1:4714468/1‑147 (MQ=16)
                                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                               <  2:2305820/150‑1 (MQ=255)
                                                                           ggATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAAACCAATCCCCCAAGGGGCGCGGGGACCGGTCAGTACCGAATTTAGCTTGACGGTGCTATCCAGCATTaaaaaa                                                                            >  2:742891/1‑147 (MQ=255)
                                                                            gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                                           <  2:2680708/150‑1 (MQ=255)
                                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                                          <  2:4376738/150‑1 (MQ=255)
                                                                              tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                         <  2:810866/150‑1 (MQ=255)
                                                                              tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                         <  2:3366028/150‑1 (MQ=255)
                                                                              tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                         <  2:2177355/150‑1 (MQ=255)
                                                                                aGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCa                                                                       <  2:3005036/150‑1 (MQ=255)
                                                                                    gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGTATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATAc                                                                   <  1:1568498/150‑1 (MQ=255)
                                                                                          gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                             <  1:4438183/150‑1 (MQ=255)
                                                                                          gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                             <  2:4625173/150‑1 (MQ=255)
                                                                                            atcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGc                                                           <  2:2530335/150‑1 (MQ=255)
                                                                                            atcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGc                                                           <  2:4755806/150‑1 (MQ=255)
                                                                                            atcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGc                                                           <  2:3344308/150‑1 (MQ=255)
                                                                                                     ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgc                                                  <  1:2123575/150‑1 (MQ=255)
                                                                                                          tGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                             <  1:2959408/150‑1 (MQ=255)
                                                                                                           gCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                            <  1:2765327/150‑1 (MQ=255)
                                                                                                               gcgcGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACACCCCAAAGGGGCGGGGGGACCGTTCAGTACGGCAATTAGCGTGTCGGTGCTATCCAGCAGTAAATAATGGCAATACTCACACGCAAAATACGCACCACCctct                                        >  2:120763/1‑150 (MQ=255)
                                                                                                                    ttGAATTTTACATCCCGTACGTTCCCCACACCCTAAACCTATACCCAAAGGGGCGAGGGGACCGTTAAGTACCGAAATTATCATTACGGTGCTATCCAGCAATAAAAAAAGGCAATAATAAAAACCAAAAAAACCAGCACCCTCTCTTCa                                   >  2:4472431/1‑150 (MQ=255)
                                                                                                                     tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                                  <  2:3769876/150‑1 (MQ=255)
                                                                                                                       aaTTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCg                                <  2:4417009/150‑1 (MQ=255)
                                                                                                                         ttttGCATCCCGTACGTTCCCCTCACCCTAACTCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                         <  2:404618/107‑1 (MQ=255)
                                                                                                                         ttttACATCCCGTACGTTCCCCTAACCCTAACCCTCTCCCCAAACGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACACTCACGCAAAATACGCAGCACCGTCTCTTCAACGCt                              >  2:391031/1‑150 (MQ=39)
                                                                                                                          tttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTg                             <  1:4179052/150‑1 (MQ=255)
                                                                                                                          ggtACATCCGGTACGTTCGGCTCCCGCTCACGCTCGCCCCTCAGGGGCGAGGGGACCGTTCAGTCCGGTATTTCGCTTGACGGTGCTATCGAGCAGTAAATACTGGCTATTCTCTCACGCATAATCCGGTGCTCCGTGTCTTCACAGCtt                             <  2:3645289/148‑2 (MQ=255)
                                                                                                                           ttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGc                            <  1:3367103/150‑1 (MQ=255)
                                                                                                                                tCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTc                       <  1:1365027/150‑1 (MQ=255)
                                                                                                                                  ccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg                     <  1:8496/150‑1 (MQ=255)
                                                                                                                                  ccGTACGTTCCCCTACCCCTAACCCGCTACCCAAAGGGGCGAGGGGACCCATCAGTACCGAATTTAGCATGACCGTGCTATCCACCATTAAATACTGGCAATACTCTCACGCCAAATCCGCCGCACCCTCTCCTCACCCCTGCGATTCgg                     >  2:2702226/1‑150 (MQ=255)
                                                                                                                                   cGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCggg                    >  2:3430764/1‑150 (MQ=255)
                                                                                                                                    gTACGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGAGGGGACCGTTAATTAAGGAATTTTTGTTTAAGGTTCTATAAATCATTAAATAATGTCAATAATATAACGCAAAATACGAAGCAACGTATATTAACCGCTGAGAttatggt                   >  2:1940161/1‑145 (MQ=12)
                                                                                                                                       cGTTCCCCTCCCCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTaa                <  2:3238800/150‑1 (MQ=255)
                                                                                                                                       cGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTAGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTaa                <  2:2804332/150‑1 (MQ=255)
                                                                                                                                       cGTTCCCCTCACCCTAACCCACTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTaa                <  2:328118/150‑1 (MQ=255)
                                                                                                                                        ggtCCCCTCACCCTCACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt               <  1:3186872/148‑1 (MQ=255)
                                                                                                                                        gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt               <  1:3509466/150‑1 (MQ=255)
                                                                                                                                             ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa          >  2:2391040/1‑150 (MQ=255)
                                                                                                                                               tCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCTCTGCGATTCGGGTTAATGCGAATc        >  1:2385775/1‑150 (MQ=255)
                                                                                                                                                 aCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc      >  2:4699457/1‑150 (MQ=255)
                                                                                                                                                 aCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc      >  2:1134002/1‑150 (MQ=255)
                                                                                                                                                   ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac    >  2:1275838/1‑150 (MQ=255)
                                                                                                                                                   ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac    >  2:2634677/1‑150 (MQ=255)
                                                                                                                                                   ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac    >  2:2434015/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAA  >  NC_000913/3504718‑3505016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: