Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,880 A→C 8.4% intergenic (+97/+55) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8800AC 8.4% 672.4 / 14.0 248intergenic (+97/+55)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (86/129);  new base C (19/6);  total (107/141)
Fisher's exact test for biased strand distribution p-value = 1.01e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

CTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTG  >  NC_000913/3504732‑3505029
                                                                                                                                                     |                                                                                                                                                     
 ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                                     <  1:2252210/150‑1 (MQ=255)
 ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAAGTTTACATCCCGTACGTTCCCCGCACCCTAACCCTCTCCCCcaa                                                                                                                                                     >  2:352745/1‑150 (MQ=255)
  tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                                                    <  1:2779306/150‑1 (MQ=255)
   ctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGATATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCTCTCCCCAAAgg                                                                                                                                                   >  2:1699164/1‑150 (MQ=255)
    tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAggg                                                                                                                                                  <  2:1814374/150‑1 (MQ=255)
        gcgcCAATCGCATCACCGGCCCTAGTGAGAATAAAAGACCGGCACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTCACCCTCTCCCCCCCGGGGCGa                                                                                                                                              <  2:1252847/150‑1 (MQ=25)
           ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                                           <  2:59571/150‑1 (MQ=255)
           ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                                           >  2:3873971/1‑150 (MQ=255)
           ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAggg                                                                                                                                           <  1:3701966/150‑1 (MQ=39)
           caaaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACGGTAGTCCGGATATGTTGCGCCAGCATCGAATCGGGCGTTGCTGGCGGGTTGATTTTTACATCCCGTTCGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                                           <  1:2648796/148‑1 (MQ=37)
            cAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACACCCCAAAGGGGCGAgggg                                                                                                                                          >  1:1657554/1‑150 (MQ=255)
            cAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCGCTACCCCAAGGGGCGAgggg                                                                                                                                          >  1:371974/1‑150 (MQ=255)
            cAATCGCATCACCTTAACAATTGATAATAAAAGACATTAACAGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCACACCCCAAAGGGGCGAgggg                                                                                                                                          >  2:2864007/1‑150 (MQ=39)
             aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGACCCCCAACCCACTCCCCCCAGGGGCggggggg                                                                                                                                         >  1:1877344/1‑149 (MQ=38)
             aaTCGCATCACCTGAACTATTGATAATAAAAGACATTAACCGTAGGCCGGCTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTGTACATCCCGTCCGGTCCCCGCCCCCAACCCCGCACCCCCCACGGGCGCggggc                                                                                                                                         >  1:2322607/1‑149 (MQ=25)
              aTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAgggtta                                                                                                                                        <  2:3154067/150‑4 (MQ=255)
              aTCGCATCACCTTAACGATTGATAATACAAGACATTCACCGGAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTGTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgggtta                                                                                                                                        <  2:2662309/150‑4 (MQ=38)
                  cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAAACCACTCCCCCAAGGGGCGAGGGGACCGtt                                                                                                                                    >  1:4983848/1‑150 (MQ=255)
                    tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCa                                                                                                                                  <  2:3481659/150‑1 (MQ=255)
                         ttcaCTCTTGATAATAAAAGACATGCACCGGAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                                                             <  2:4504823/147‑1 (MQ=38)
                          tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCCAAAGGGCCAGGGGACCGTGCAATACgg                                                                                                                            >  1:1112763/1‑150 (MQ=38)
                            aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                                                          <  1:4587470/150‑1 (MQ=255)
                              tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAtt                                                                                                                        <  2:3695332/150‑1 (MQ=255)
                                ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACAACCCCAAGGGGCGAGGGGCCCGGTCAGTACGGAAATTa                                                                                                                      >  1:1945990/1‑150 (MQ=38)
                                ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACAACCAAACCCACTACCCAAATGGGCGAGGGGACCCTTCAGTACGGAATTTa                                                                                                                      >  1:3864422/1‑150 (MQ=38)
                                        aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGAATTTTACATCCCGTACGTTGCCCGCACCCAAAACCACACCCCCAAGGGGCGGGGGGGCCCGTGAGTGAGGAAGTTAGCGTGTCg                                                                                                              >  1:973418/1‑150 (MQ=21)
                                         aaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCTCTCCCCAAACGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACgg                                                                                                             >  2:4141818/1‑150 (MQ=255)
                                         aaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCTACCCAAATGGGCGAGGGGACCGGTCAGTAACGAATTTAGCTTGACgg                                                                                                             >  2:673406/1‑150 (MQ=39)
                                           aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                                           <  2:2363891/150‑1 (MQ=255)
                                            gACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                                           <  2:299478/150‑1 (MQ=255)
                                            gACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGc                                                                                                          <  2:2057266/150‑1 (MQ=255)
                                            gACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGAATTTTACATCCCGTACGGTCCCCACACCCAAACCCACACCCCCAACGGGCCCGGGGCCCGGTCAGTAAGGCAAGTAGCTTGACGGTGc                                                                                                          >  1:3392928/1‑150 (MQ=25)
                                                 tAACCGTCGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTTCGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATcc                                                                                                     <  1:716191/150‑1 (MQ=255)
                                                    ccGTTGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGc                                                                                                  <  1:1308734/150‑1 (MQ=255)
                                                       tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGTATTTTAAATCCCGTACGGTACCCCCACCCGAAACCAAAACCCAAAGGGGGGAGGGGGCCGGTGCGGACGGAAATTTTCTTGTAGGGGCTATCCAGccat                                                                                               >  1:4714468/1‑147 (MQ=16)
                                                          gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                                            <  2:2305820/150‑1 (MQ=255)
                                                             ggATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAAACCAATCCCCCAAGGGGCGCGGGGACCGGTCAGTACCGAATTTAGCTTGACGGTGCTATCCAGCATTaaaaaa                                                                                         >  2:742891/1‑147 (MQ=255)
                                                              gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                                                        <  2:2680708/150‑1 (MQ=255)
                                                               attcaacgcgcCAGCATCGCCGGATGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCATGACGGTGCTATCCAGCCGTAAAAACTg                                                                                       >  2:4702036/8‑150 (MQ=25)
                                                                tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                                      <  2:899307/150‑1 (MQ=255)
                                                                tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                                      <  2:810866/150‑1 (MQ=255)
                                                                            gcatcgcctcCGGCGATGCTGGCGCGTTGAATTGTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                                          <  1:4951324/150‑1 (MQ=255)
                                                                            gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                                          <  1:4438183/150‑1 (MQ=255)
                                                                            gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                                          <  1:2502572/150‑1 (MQ=255)
                                                                            gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCACTCCCCAAACGGGCGAGGGGACCGGTCAGTACGGAAATTATCTTGACGGTGCGAACCAGCAATAAATACAGGCAATaaacgcac                                                                          >  2:1441137/1‑143 (MQ=255)
                                                                            gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                                          <  1:1468397/150‑1 (MQ=255)
                                                                              atcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGc                                                                        <  2:2530335/150‑1 (MQ=255)
                                                                              atcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGc                                                                        <  2:2709894/150‑1 (MQ=255)
                                                                              atcgcatcCGGCGATGCTGGCGCGTTGAATTGTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGc                                                                        <  2:3344356/150‑1 (MQ=255)
                                                                                  catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCACTCCCCAAAGGGGCGCGGGGCCCGGTCAGTACGGAATTTAGCGTGTCGGTGCTATCCAGCATTAAATACTGGCAATAATCTAACGCaaaa                                                                    >  2:4464342/1‑150 (MQ=255)
                                                                                       ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgc                                                               <  1:3063253/150‑1 (MQ=255)
                                                                                       ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAATAAATACTGGCAATACTCTCAAGCAAAATCCgc                                                               >  2:1182410/1‑150 (MQ=255)
                                                                                           aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagca                                                           <  1:273938/150‑1 (MQ=255)
                                                                                           aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagca                                                           <  2:5014854/150‑1 (MQ=255)
                                                                                            tGCTGGCGCGTTGAAATTTACATCCCGAACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACCCAAAATCCGCAGCAc                                                          <  1:1769217/150‑1 (MQ=255)
                                                                                               tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                                                       <  2:4598745/150‑1 (MQ=255)
                                                                                                    cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCtt                                                  <  2:5004548/150‑1 (MQ=255)
                                                                                                         aaTTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCg                                             <  2:4417009/150‑1 (MQ=255)
                                                                                                           ttttGCATCCCGTACGTTCCCCTCACCCTAACTCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                                      >  1:404618/1‑107 (MQ=255)
                                                                                                           ttttGCATCCCGTACGTTCCCCTCACCCTAACTCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                                                      <  2:404618/107‑1 (MQ=255)
                                                                                                            tttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTg                                          <  1:4179052/150‑1 (MQ=255)
                                                                                                            ggtACATCCGGTACGTTCGGCTCCCGCTCACGCTCGCCCCTCAGGGGCGAGGGGACCGTTCAGTCCGGTATTTCGCTTGACGGTGCTATCGAGCAGTAAATACTGGCTATTCTCTCACGCATAATCCGGTGCTCCGTGTCTTCACAGCtt                                          <  2:3645289/148‑2 (MQ=255)
                                                                                                             ttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGc                                         <  1:3367103/150‑1 (MQ=255)
                                                                                                                  tCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTc                                    <  1:1365027/150‑1 (MQ=255)
                                                                                                                    ccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg                                  <  1:8496/150‑1 (MQ=255)
                                                                                                                    ccGTACGTTCCCCACACCCTAAACCACAACCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACGGTGCTAACCAGCATTAAATAAAGGCAATACACTCACCCAAAATACGCAGCACCGTCTCTTCACCGCTGCGATTAgg                                  >  2:3212267/1‑150 (MQ=255)
                                                                                                                     cGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCggg                                 >  2:3430764/1‑150 (MQ=255)
                                                                                                                      gTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt                                <  2:2167444/150‑1 (MQ=255)
                                                                                                                      gTACGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGAGGGGACCGTTAATTAAGGAATTTTTGTTTAAGGTTCTATAAATCATTAAATAATGTCAATAATATAACGCAAAATACGAAGCAACGTATATTAACCGCTGAGAttatggt                                >  2:1940161/1‑145 (MQ=12)
                                                                                                                         cGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTAGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTaa                             <  2:2804332/150‑1 (MQ=255)
                                                                                                                         cGTTCCCCTCACCCTAACCCACTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTaa                             <  2:328118/150‑1 (MQ=255)
                                                                                                                          ggtCCCCTCACCCTCACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt                            <  1:3186872/148‑1 (MQ=255)
                                                                                                                          gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt                            <  1:3509466/150‑1 (MQ=255)
                                                                                                                            tCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGc                          >  2:4345216/1‑150 (MQ=255)
                                                                                                                               ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa                       >  2:2391040/1‑150 (MQ=255)
                                                                                                                                cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAAt                      >  1:1253578/1‑150 (MQ=255)
                                                                                                                                 tCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCTCTGCGATTCGGGTTAATGCGAATc                     >  1:2385775/1‑150 (MQ=255)
                                                                                                                                   aCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc                   >  2:1134002/1‑150 (MQ=255)
                                                                                                                                     ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac                 >  2:1275838/1‑150 (MQ=255)
                                                                                                                                     ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac                 >  2:3377491/1‑150 (MQ=255)
                                                                                                                                     ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac                 >  2:2434015/1‑150 (MQ=255)
                                                                                                                                         aCCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAACATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATg             <  2:708057/150‑1 (MQ=255)
                                                                                                                                            ccTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGtt           >  1:4630698/1‑150 (MQ=255)
                                                                                                                                             ctctCCCCATAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc          <  1:2649268/150‑1 (MQ=255)
                                                                                                                                             ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCTATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc          <  1:1017135/150‑1 (MQ=255)
                                                                                                                                             ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc          <  1:2952037/150‑1 (MQ=255)
                                                                                                                                             ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc          >  1:2110924/1‑150 (MQ=255)
                                                                                                                                             ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTGGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc          <  2:4118022/150‑1 (MQ=255)
                                                                                                                                              tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCt         >  2:1034794/1‑150 (MQ=255)
                                                                                                                                              tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCt         >  1:3564301/1‑150 (MQ=255)
                                                                                                                                                  cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGAtt     >  2:4661595/1‑150 (MQ=255)
                                                                                                                                                  cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGAtt     >  2:3794173/1‑150 (MQ=255)
                                                                                                                                                  cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGAtt     >  2:2782290/1‑150 (MQ=255)
                                                                                                                                                  cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGAtt     >  1:4535515/1‑150 (MQ=255)
                                                                                                                                                  cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGAtt     >  2:180096/1‑150 (MQ=255)
                                                                                                                                                   ccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGTTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtg    >  1:544053/1‑150 (MQ=255)
                                                                                                                                                   ccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtg    >  2:2211929/1‑150 (MQ=255)
                                                                                                                                                    cAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtgt   <  2:1657554/150‑1 (MQ=255)
                                                                                                                                                    cAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtgt   >  1:219311/1‑150 (MQ=255)
                                                                                                                                                     aaaGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtgtg  >  2:407510/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGTG  >  NC_000913/3504732‑3505029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: