Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,481 C→G 6.9% intergenic (+36/+51) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,4810CG 6.9% 961.5 / 50.6 277intergenic (+36/+51)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base C (101/155);  new base G (8/11);  total (111/166)
Fisher's exact test for biased strand distribution p-value = 8.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 292 total aligned reads displayed.

ACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAA  >  NC_000913/3959333‑3959630
                                                                                                                                                    |                                                                                                                                                     
aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                                                      <  2:1408090/150‑1 (MQ=255)
aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                                                      <  1:4883879/150‑1 (MQ=255)
aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                                                      <  2:1310985/150‑1 (MQ=255)
aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                                                      <  2:522267/150‑1 (MQ=255)
 cGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACag                                                                                                                                                     <  2:179070/150‑1 (MQ=255)
 cGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACag                                                                                                                                                     <  1:1931791/150‑1 (MQ=255)
  gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagg                                                                                                                                                    <  1:2415458/150‑1 (MQ=255)
      aaCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggaga                                                                                                                                                <  1:3042782/150‑1 (MQ=255)
      aaCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggaga                                                                                                                                                <  1:323712/150‑1 (MQ=255)
       aCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagag                                                                                                                                               <  2:3081792/150‑1 (MQ=255)
       aCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagag                                                                                                                                               <  1:3157899/150‑1 (MQ=255)
        cTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagagg                                                                                                                                              <  2:1491705/150‑1 (MQ=255)
        cTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagagg                                                                                                                                              <  2:185484/150‑1 (MQ=255)
         tGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCTCACCCCCcgggagaggg                                                                                                                                             >  1:3584945/1‑150 (MQ=255)
         tGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGATATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCTCTCCCACagggagaggg                                                                                                                                             <  1:4144386/150‑1 (MQ=255)
          gCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggga                                                                                                                                            <  1:3331392/150‑1 (MQ=255)
             tGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagagggtcgg                                                                                                                                         <  2:2870619/150‑5 (MQ=255)
              gATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagagggtcggg                                                                                                                                        <  2:3766352/150‑6 (MQ=255)
              gATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGaaa                                                                                                                                        <  2:558698/150‑1 (MQ=255)
               aTGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGaaaa                                                                                                                                       <  2:3215140/150‑1 (MQ=255)
                   gAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagagggt                                                                                                                                            >  1:4541318/1‑140 (MQ=255)
                    aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTc                                                                                                                                  >  2:1247379/1‑150 (MQ=255)
                    aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGGAAACACTc                                                                                                                                  >  2:5009764/1‑150 (MQ=255)
                    aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGCTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCTCTCCCACACGGGGCGGGGGTAAacacgg                                                                                                                                  >  1:912808/1‑148 (MQ=255)
                      cGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCaa                                                                                                                                <  1:4069159/150‑1 (MQ=255)
                      cGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCaa                                                                                                                                <  2:4113997/150‑1 (MQ=255)
                       gAAGCGATTCGCAGCCATGCGATTGATCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATTCCCTCACCCCGACCCTCTCCCACAGCGAGAGGGAGAAAACACACAAg                                                                                                                               >  1:4297716/1‑150 (MQ=255)
                       gAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGTGAGGGAGAAAACACTCAAg                                                                                                                               <  1:4493678/150‑1 (MQ=255)
                                       aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                                               <  1:2543103/150‑1 (MQ=255)
                                       aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                                               <  1:1007256/150‑1 (MQ=255)
                                        tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCCACCCACACACACACGGCGAGGGCGTAAAAAAACAAAGCCGTCTCCgggtgggg                                                                                                              >  1:1336215/1‑145 (MQ=255)
                                         gCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACAACCCCACCCACTCCCCCCGGGAGAGGGAGAAAAAACACAAGGCCATATACGGGAGAAgg                                                                                                             >  2:1628779/1‑150 (MQ=255)
                                           gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGTTGGCCTAAACCCGTCCCTCTCCCGCAGGGGGTGGGAGTAAACACTGAATGGCTTCTCCTGGGGAAgggg                                                                                                           >  2:3401233/1‑148 (MQ=255)
                                            aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATCTGACAGATAGGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt                                                                                                          <  2:4699709/150‑1 (MQ=255)
                                            aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt                                                                                                          <  1:1144866/150‑1 (MQ=255)
                                            aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACGCAAGGCCTTCTCCAGGAGAAGGCCt                                                                                                          <  1:4985636/150‑1 (MQ=255)
                                             ttGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCtt                                                                                                         <  2:38134/150‑1 (MQ=255)
                                                 gCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTa                                                                                                     <  1:2620614/150‑1 (MQ=255)
                                                 gCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTa                                                                                                     <  1:15601/150‑1 (MQ=255)
                                                   aggtaggtAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCTCACAGGGAGAGGGAGAATACACTCAAGGCCTTATCCTGGAGAAGGCCTTGCTAtt                                                                                                   <  1:4737179/150‑1 (MQ=255)
                                                     gtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCTGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAg                                                                                                 >  1:1979978/1‑150 (MQ=255)
                                                        ggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTg                                                                                              <  1:3936116/150‑1 (MQ=255)
                                                          taAGAAACTGCGCGGCTATATGACAGATATGAACCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                                                                                            <  2:2011629/150‑1 (MQ=255)
                                                           aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                                                           <  1:4777417/150‑1 (MQ=255)
                                                            aGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCACTCCCACAGGGAGTGGGAGAAAACACAAAATGCCTTCTCCTGGAGAATGCCATGCTATTATTTGCGGt                                                                                          >  1:1675226/1‑150 (MQ=255)
                                                              aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCAAACCCACAGGGCGATGGCGAAAACAAGAAACGCCGTCTCCAGGCGAAGGCCGGGCAATTAGTTGCGGGAc                                                                                        >  1:5112490/1‑150 (MQ=255)
                                                                  tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGGTAAGTGCGCGCTGAGGCCCGCACCCCGACCCACTCCCCCAGGGCGAGGGCGAAAACACACAACGCCTTCACCAGGCGAACGCCTTGCTAGTAGTTGCGGGACAGcc                                                                                    >  2:4229138/1‑149 (MQ=255)
                                                                   gcgcGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAc                                                                                   <  1:3464807/150‑1 (MQ=255)
                                                                     gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCt                                                                                 <  1:1052053/150‑1 (MQ=255)
                                                                       ggCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGGTGCTGTACCGCAACtaa                                                                               <  1:180710/150‑3 (MQ=255)
                                                                         cTATATGACAGATATGAAACGTATTGCTGTAGCGGGTTAAGTGCGCGCTGATGCCCGCAAACCCACCCACACACACACGGTGTGGGCTAAAACAAACAATGCCATCTACTTGAGAACGCCATGATCATAATTGAGTTACAGCAccaaaaa                                                                             >  1:5065593/1‑145 (MQ=255)
                                                                           atatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatga                                                                           <  1:4469129/150‑1 (MQ=255)
                                                                              tGACAGATATGACACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                        <  1:2290303/150‑1 (MQ=255)
                                                                              tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCACGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                        <  2:2548613/150‑1 (MQ=255)
                                                                               gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgtg                                                                       <  2:3474248/150‑1 (MQ=255)
                                                                               gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACACAAGGCCTTCTCCTGGGGAAGGCCTTGCTATTATTTGCGGTACAGCACCTTAATGTtgtg                                                                       >  2:3669385/1‑150 (MQ=255)
                                                                                tccgATCTGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                                                      <  1:1622220/147‑1 (MQ=255)
                                                                                  aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTACCACAGGGGGGGGGCGAAAACACGCAAGGCCTTCTCCGGGAGGAGGCCGTGCTATTAGTTGCGGGTCAGCACCATAATGTTGTGgta                                                                    >  2:3713116/1‑150 (MQ=37)
                                                                                  aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCACCCACAACCCCAGGGGGAGGGAGAAAACAAAAAAGGCCATCTACGGGAGAAGGCCGTGCTATTATTTTAGGTAAACCACCATAATGATTTtata                                                                    >  2:3894979/1‑150 (MQ=255)
                                                                                    atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                                  <  2:3093187/150‑1 (MQ=255)
                                                                                    atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                                  <  1:1962433/150‑1 (MQ=255)
                                                                                        gAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCACACCCACACGGCGCGGGAGCAAACACACAAGGCCGTCACCGGGAGGAGGCCGTGCTATTAGTTTCGGTACAGCACCAGAATTATGTGATATGCGaa                                                              >  1:5009304/1‑150 (MQ=255)
                                                                                         aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCACACCCCCACGGGGAGGGGGAAAACACAAAAGGCCTTCACCTGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCATAATGATGTGATATACGAAc                                                             >  2:1052312/1‑150 (MQ=255)
                                                                                         aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCTCACCCACAGGGCGAGGGAGAAAACACGAAAGGCCATATCCTGGAGAAGGCCGTGCTATTATTTGCGGTAAAGCCCCATAATGAAGTGATAAACGAAc                                                             >  2:1171574/1‑150 (MQ=255)
                                                                                          aaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCACCCACTCCCACACGGCGAGGGAGCAAACACACAATGCCGTCACCAGGAGGACGCCGTGCTATTAGTTTCGGGACCCCACCATAAAGAAGTGATAACCCAAcg                                                            >  2:1563998/1‑149 (MQ=255)
                                                                                           aCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTACCACAGGGAGAGGGAGAAAACACACAAGGCCTTCTCCTGGAGAACGCCTTGCAATTAGTTGCGGTACAGCACCATAATGATGTGATATACGaaaag                                                           >  2:2795941/1‑147 (MQ=38)
                                                                                            cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                          <  1:3872074/150‑1 (MQ=255)
                                                                                            cGTATTGCTGTTGCGGGTTAAGGGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                          <  1:1307937/150‑1 (MQ=255)
                                                                                                ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCTCACCCACAGGGAGAGGGAGAAAACACACAAGGCCGTATCCTGGAGAAGGCCATGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATACGAACGGGgtgt                                                      >  2:4176163/1‑150 (MQ=38)
                                                                                                  gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGc                                                    <  2:3511781/150‑1 (MQ=255)
                                                                                                      ttGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACAACCACAGGGCGAGGGCGAAAAAACACAAGGCCAACTACAGGGGAAGGCCATGGGAATATTTTCGGTACAGCCCCGAAAATAAGTGATAAACGAACAGGGGGTTCAGCg                                                >  2:2276264/1‑150 (MQ=255)
                                                                                                         cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc                                             <  2:1616777/150‑1 (MQ=255)
                                                                                                             ttAAGTGCGCGCTGATGCCCTCACCCCCACCCACACCCACACGGAGAGGGAGAAAACACACAAGGCCATCTCCAGGAGAACGCCGTGCTAATAGTTGCGGGACAGCACCATAAAGAAGTGCTATACGAACAGGGTGTGCCGCGGGCCggg                                         >  2:3996535/1‑149 (MQ=25)
                                                                                                             ttAAGTGCGCGCTGATGCCCACACCCCCACCCACACCCACAGGGAGAGGGGGTAAACAAACAAGGCCTTATCCGGGAGAAGGCCGTGGTATTAGTTGCGGTTCACCACCATAATGATGTGATATCCGGACAGGGTGTGCAGCGGGcccgg                                         >  2:1006352/1‑147 (MQ=255)
                                                                                                             ttAAGTGCGCGCTGAGGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt                                         <  1:4685678/150‑1 (MQ=255)
                                                                                                                aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                      <  1:2758771/150‑1 (MQ=255)
                                                                                                                aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                      <  1:759860/150‑1 (MQ=255)
                                                                                                                     gcgcTGATGCTCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                 <  1:845065/150‑1 (MQ=255)
                                                                                                                     gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                 <  2:2724095/150‑1 (MQ=255)
                                                                                                                     gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                 <  1:291757/150‑1 (MQ=255)
                                                                                                                     gcgcTGATGCCCCCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCACGGCCTTCGCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                 <  2:4864737/150‑1 (MQ=255)
                                                                                                                          gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                            <  1:4382248/150‑1 (MQ=255)
                                                                                                                             gCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGa                         <  2:4347457/150‑1 (MQ=255)
                                                                                                                             gCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGa                         <  1:2707727/150‑1 (MQ=255)
                                                                                                                                gtCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGTCGGCCTGGCTATTAGTTGCGGTACAGCACCTTCATGATGTGATATCCGAACGGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGAcca                      >  2:249133/2‑148 (MQ=255)
                                                                                                                                  cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga                    <  1:327778/150‑1 (MQ=255)
                                                                                                                                  cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga                    <  2:1727929/150‑1 (MQ=255)
                                                                                                                                    ccccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAgaga                  <  2:4965090/150‑1 (MQ=255)
                                                                                                                                     cccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCACGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaa                 <  2:4523300/150‑1 (MQ=255)
                                                                                                                                     cccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGACCCGGACAAGAGaa                 >  1:77726/1‑150 (MQ=255)
                                                                                                                                      ccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaa                <  1:1700910/150‑1 (MQ=255)
                                                                                                                                       cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa               >  2:4571457/1‑150 (MQ=255)
                                                                                                                                         tcccTCTCCCTGTGGGAGAGGGTGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAAcc             >  1:3520120/2‑150 (MQ=255)
                                                                                                                                          cccTCTCCCTGTGGGAGAGGGTGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGGACCGGACAAGAGAAAACCa            >  1:2864737/1‑150 (MQ=255)
                                                                                                                                           ccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc           >  2:2222133/1‑150 (MQ=255)
                                                                                                                                             tctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACtt         >  2:237483/1‑150 (MQ=255)
                                                                                                                                                 ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTGTATc     >  1:595441/1‑150 (MQ=255)
                                                                                                                                                   acaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGa   >  1:2363461/1‑150 (MQ=255)
                                                                                                                                                   acaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGa   >  1:2243201/1‑150 (MQ=255)
                                                                                                                                                    caGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGaa  <  2:902397/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAA  >  NC_000913/3959333‑3959630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: