Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,336,988 A→T 5.0% V15D (GTC→GAC)  adiC ← arginine:agmatine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,336,9880AT 5.0% 964.5 / 27.2 269V15D (GTC→GAC) adiCarginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base A (136/119);  new base T (5/9);  total (141/128)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 271 total aligned reads displayed.

CGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTT  >  NC_000913/4336840‑4337137
                                                                                                                                                    |                                                                                                                                                     
cGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGAc                                                                                                                                                      >  1:4132639/1‑150 (MQ=255)
   cATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGTCACCCTGATGGTGTCggg                                                                                                                                                   <  2:5084159/150‑1 (MQ=255)
   cATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGTCACCCTGATGGTGTCggg                                                                                                                                                   <  1:2338299/150‑1 (MQ=255)
   cATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACggg                                                                                                                                                   >  2:1207784/1‑150 (MQ=255)
     ttttGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGa                                                                                                                                                 <  1:875568/150‑1 (MQ=255)
       ttGGCGGATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCACCCATCCATAACTGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGAtt                                                                                                                                               <  1:1385761/150‑1 (MQ=255)
          gCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAg                                                                                                                                            <  2:1635365/150‑1 (MQ=255)
          gCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAg                                                                                                                                            <  2:1904552/150‑1 (MQ=255)
          gCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAg                                                                                                                                            >  2:605780/1‑150 (MQ=255)
          gCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAg                                                                                                                                            >  2:3433514/1‑150 (MQ=255)
           cGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGc                                                                                                                                           <  2:409970/150‑1 (MQ=255)
              ataCCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCa                                                                                                                                        >  1:3423116/1‑150 (MQ=255)
               taCCATCGAGAGCCCCAGTGCACCGATACTCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGTCACCCTGATGGTGTCGGGGAATattatggg                                                                                                                                       <  2:2726172/150‑8 (MQ=38)
                  cATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACttt                                                                                                                                    >  1:33387/1‑150 (MQ=255)
                  cATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACttt                                                                                                                                    >  2:2466285/1‑150 (MQ=255)
                  cATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACttt                                                                                                                                    >  2:3798522/1‑150 (MQ=255)
                   aTCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTtg                                                                                                                                   <  2:3757501/150‑1 (MQ=255)
                    tCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTtgt                                                                                                                                  <  2:2022892/150‑1 (MQ=255)
                      gagaGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGa                                                                                                                                <  1:1098952/150‑1 (MQ=255)
                      gagaGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGa                                                                                                                                <  1:5083484/150‑1 (MQ=255)
                        gagCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagc                                                                                                                              <  1:2882212/150‑1 (MQ=255)
                           ccccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatc                                                                                                                           >  1:2925623/1‑150 (MQ=255)
                           ccccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatc                                                                                                                           >  1:662891/1‑150 (MQ=255)
                           ccccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATGAAGCCCACTTTGTGagcatc                                                                                                                           >  2:1961059/1‑150 (MQ=255)
                            cccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatca                                                                                                                          <  1:1479033/150‑1 (MQ=255)
                              cAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagc                                                                                                                        >  1:1539807/1‑150 (MQ=255)
                              cAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagc                                                                                                                        >  1:4544300/1‑150 (MQ=255)
                              cAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagc                                                                                                                        <  1:4766654/150‑1 (MQ=255)
                              cAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagc                                                                                                                        <  1:1043613/150‑1 (MQ=255)
                                     ccGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGaa                                                                                                                 <  2:2158553/150‑1 (MQ=255)
                                       gATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGa                                                                                                               <  1:3815723/150‑1 (MQ=255)
                                               tCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTa                                                                                                       <  1:3576368/150‑1 (MQ=255)
                                               tCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTa                                                                                                       >  2:3298095/1‑150 (MQ=255)
                                                cACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaa                                                                                                      >  2:1045346/1‑150 (MQ=255)
                                                 accaaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaaa                                                                                                     <  1:1837427/150‑1 (MQ=255)
                                                 accaaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaaa                                                                                                     <  2:3979513/150‑1 (MQ=255)
                                                 accaaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaaa                                                                                                     <  1:3978692/150‑1 (MQ=255)
                                                  ccaaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAAc                                                                                                    >  2:4024956/1‑150 (MQ=255)
                                                  ccaaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAAc                                                                                                    <  1:1207784/150‑1 (MQ=255)
                                                   caaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAg                                                                                                                <  2:1181989/137‑1 (MQ=255)
                                                    aaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTc                                                                                                  >  1:2977125/1‑150 (MQ=255)
                                                    aaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTc                                                                                                  <  2:1775592/150‑1 (MQ=255)
                                                     accaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTcc                                                                                                 <  2:2669151/150‑1 (MQ=255)
                                                           catAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGaa                                                                                           <  2:4651436/150‑1 (MQ=255)
                                                           catAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGaa                                                                                           >  2:3570528/1‑150 (MQ=255)
                                                           catAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGaa                                                                                           >  1:3267969/1‑150 (MQ=255)
                                                           catAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCACACTTTGTGAGCATCAGCATAAGAAGACATAATTAAACTCCTGCGaa                                                                                           >  1:316890/1‑150 (MQ=255)
                                                            atAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAg                                                                                          <  1:2805136/150‑1 (MQ=255)
                                                            atAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAg                                                                                          >  1:3141191/1‑150 (MQ=255)
                                                              aaaTGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGc                                                                                        >  1:4966033/1‑150 (MQ=255)
                                                                  ggCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGc                                                                                    <  2:3711695/150‑1 (MQ=255)
                                                                   gCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCt                                                                                   >  2:4513517/1‑150 (MQ=255)
                                                                     aaTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTc                                                                                 <  2:109495/150‑1 (MQ=255)
                                                                     aaTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTc                                                                                 <  1:1525615/150‑1 (MQ=255)
                                                                      aTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCg                                                                                >  2:1286782/1‑150 (MQ=255)
                                                                      aTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCg                                                                                >  1:2054169/1‑150 (MQ=255)
                                                                       tCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGt                                                                               <  2:4768029/150‑1 (MQ=255)
                                                                         ccGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGa                                                                             >  1:4080050/1‑150 (MQ=255)
                                                                         ccGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGa                                                                             <  1:4370661/150‑1 (MQ=255)
                                                                          cGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGACGGCGAGCTTCGTGAc                                                                            >  2:3489808/1‑150 (MQ=255)
                                                                          cGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAc                                                                            >  2:2679835/1‑150 (MQ=255)
                                                                          cGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATAAGGGTGACGGGGATTAAGCCCACGTTGTGAGCATCAGCATCCGACGACATAATTAAACTCCTGCGAAGCCGAGATTCGTGAc                                                                            >  2:4909061/1‑150 (MQ=255)
                                                                            ccAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACaa                                                                          <  2:2989171/150‑1 (MQ=255)
                                                                            ccAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACaa                                                                          <  2:1539807/150‑1 (MQ=255)
                                                                                  gagGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAAtt                                                                    <  2:1556276/150‑1 (MQ=255)
                                                                                   agGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTa                                                                   >  1:907430/1‑150 (MQ=255)
                                                                                     gCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATaa                                                                                                          <  2:2310959/109‑1 (MQ=255)
                                                                                     gCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATaa                                                                                                          >  1:2310959/1‑109 (MQ=255)
                                                                                     gCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACg                                                                 >  1:551040/1‑150 (MQ=255)
                                                                                     gCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACg                                                                 >  2:4732323/1‑150 (MQ=255)
                                                                                     gCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACg                                                                 >  2:3810773/1‑150 (MQ=255)
                                                                                                aGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATAc                                                      >  1:1005553/1‑150 (MQ=255)
                                                                                                 ggTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACg                                                     >  1:4493331/1‑150 (MQ=255)
                                                                                                  gTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGc                                                    <  2:2811322/150‑1 (MQ=255)
                                                                                                       aGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCa                                               >  1:2406029/1‑150 (MQ=255)
                                                                                                       aGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCa                                               >  2:1331874/1‑150 (MQ=255)
                                                                                                       aGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCa                                               >  1:3318992/1‑150 (MQ=255)
                                                                                                       aGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTGTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCa                                               >  2:291114/1‑150 (MQ=255)
                                                                                                        gAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCAt                                              >  2:354471/1‑150 (MQ=255)
                                                                                                         aaaaaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCAtt                                             >  2:3919818/1‑150 (MQ=255)
                                                                                                            aaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa                                          >  2:4209281/1‑150 (MQ=255)
                                                                                                               acCTGACCCCATAATATTCCCCGACACCCTCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAg                                       <  1:3665093/150‑1 (MQ=255)
                                                                                                               acCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAg                                       <  1:1877798/150‑1 (MQ=255)
                                                                                                                ccTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGt                                      <  2:1015839/150‑1 (MQ=255)
                                                                                                                 cTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGtt                                     >  2:878002/1‑150 (MQ=255)
                                                                                                                   gACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGa                                   >  1:804588/1‑150 (MQ=255)
                                                                                                                      cccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatt                                >  1:3913704/1‑150 (MQ=255)
                                                                                                                      cccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatt                                >  1:1822342/1‑150 (MQ=255)
                                                                                                                      cccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatt                                >  1:1645533/1‑150 (MQ=255)
                                                                                                                       ccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatta                               >  1:2998708/1‑150 (MQ=255)
                                                                                                                           aataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATtattat                           >  2:1260336/1‑150 (MQ=255)
                                                                                                                            tattcccccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATc                          <  2:3600263/145‑1 (MQ=255)
                                                                                                                               ttCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAc                       >  1:4914612/1‑150 (MQ=255)
                                                                                                                                tCCCCGTCACCCTGATGGTGTCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAcc                      >  1:3485146/1‑150 (MQ=255)
                                                                                                                                tCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGAGAGTTGAATTATTATCGAcc                      >  1:2740078/1‑150 (MQ=255)
                                                                                                                                 ccccGTCACCCTGATGGTGTCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCg                     >  2:4304025/1‑150 (MQ=255)
                                                                                                                                 ccccGTCACCCTGATGGTGTCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCgg                    >  2:523960/1‑150 (MQ=255)
                                                                                                                                  cccGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCgg                    >  2:3342102/1‑150 (MQ=255)
                                                                                                                                        acCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATc              >  2:2053832/1‑150 (MQ=255)
                                                                                                                                                 gTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGcc     >  2:136518/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTT  >  NC_000913/4336840‑4337137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: