Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 281,946 A→T 7.2% M13K (ATG→AAG)  yagA ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913281,9460AT 7.2% 881.6 / 3.3 289M13K (ATG→AAG) yagACP4‑6 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (167/86);  new base T (3/28);  total (171/118)
Fisher's exact test for biased strand distribution p-value = 1.16e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

GGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCT  >  NC_000913/281797‑282095
                                                                                                                                                     |                                                                                                                                                     
gccggTCTTCAGGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACa                                                                                                                                                       >  2:3693595/3‑150 (MQ=255)
 gcggTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACAt                                                                                                                                                      <  2:573900/150‑1 (MQ=255)
  cggTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATg                                                                                                                                                     <  2:1486752/150‑1 (MQ=255)
   ggTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATgg                                                                                                                                                    >  2:4656785/1‑150 (MQ=255)
    gTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGt                                                                                                                                                   <  1:1012465/150‑1 (MQ=255)
     tCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTa                                                                                                                                                  <  2:413648/150‑1 (MQ=255)
     tCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTa                                                                                                                                                  >  1:449385/1‑150 (MQ=255)
     tCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTa                                                                                                                                                  >  1:4781176/1‑150 (MQ=255)
        tGACGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTAtct                                                                                                                                               <  2:4232088/150‑1 (MQ=255)
        tGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTAtct                                                                                                                                               >  1:1474087/1‑150 (MQ=255)
        tGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTAtct                                                                                                                                               >  1:4725612/1‑150 (MQ=255)
           aGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTAtctctc                                                                                                                                            <  1:1555919/150‑1 (MQ=255)
            gACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCg                                                                                                                                           >  1:196449/1‑150 (MQ=255)
            gACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCg                                                                                                                                           <  2:5325715/150‑1 (MQ=255)
             aCCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGc                                                                                                                                          >  1:173791/1‑150 (MQ=255)
             aCCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGc                                                                                                                                          >  2:5273331/1‑150 (MQ=255)
               cggcggCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCAt                                                                                                                                        >  1:5242248/1‑150 (MQ=255)
                  cggcACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATccc                                                                                                                                     >  2:3397917/1‑150 (MQ=255)
                   ggcACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCa                                                                                                                                    <  1:283797/150‑1 (MQ=255)
                   ggcACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGAGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCTCATCCCa                                                                                                                                    <  1:959126/150‑1 (MQ=255)
                    gcACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAg                                                                                                                                   >  1:3510760/1‑150 (MQ=255)
                     cACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAgg                                                                                                                                  <  1:3064514/150‑1 (MQ=255)
                     cACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAgg                                                                                                                                  >  1:5049396/1‑150 (MQ=255)
                      aCCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAggg                                                                                                                                 <  1:2533479/150‑1 (MQ=255)
                      aCCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAggg                                                                                                                                 >  2:717411/1‑150 (MQ=255)
                        cTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCa                                                                                                                               >  2:2616927/1‑150 (MQ=255)
                        cTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCa                                                                                                                               >  1:5434900/1‑150 (MQ=255)
                         ttCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCTTCCCAGGGCAt                                                                                                                              >  1:3391773/1‑150 (MQ=255)
                         ttCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGGATCGCTCGCATCCCAGGGCAt                                                                                                                              >  1:2435/1‑150 (MQ=255)
                          tCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATa                                                                                                                             >  1:3329689/1‑150 (MQ=255)
                          tCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATa                                                                                                                             >  1:4846484/1‑150 (MQ=255)
                          tCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATa                                                                                                                             <  1:3783157/150‑1 (MQ=255)
                                gCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGAc                                                                                                                       <  2:3544756/150‑1 (MQ=255)
                                gCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGAc                                                                                                                       >  1:2928360/1‑150 (MQ=255)
                                    aGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCa                                                                                                                   >  2:1395603/1‑150 (MQ=255)
                                    aGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCa                                                                                                                   >  2:2295945/1‑150 (MQ=255)
                                    aGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCa                                                                                                                   >  1:2699665/1‑150 (MQ=255)
                                       gcTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATaa                                                                                                                >  2:2280113/1‑150 (MQ=255)
                                         tGGAGCCACTTGTAGCCGGTGGCTGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCTAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAAc                                                                                                              <  2:4964701/150‑1 (MQ=255)
                                              ccACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGtt                                                                                                         >  1:3196839/1‑150 (MQ=255)
                                                aCTTGTTGCCGGTGGCTGGTGTATTGCGGTAGCGACGGCAGTGGGAACGTTTGTTCGCCCCGTCCTGCTAGGCGAACAAAACATACTCAGTACGTAAGGACATGGTATCTCTCGCTTCCGTGGGATTAAGCGACTCCATAAACGGGTTCt                                                                                                       <  2:3192147/150‑1 (MQ=255)
                                                  ttgttgCCGGTGGCTGGTTAAATGCCTAAGCGACGGCAGAGGTAACGGATGTTCGCCCCGTCCTGCGAGGCGAAAAAAAAAAACTCAGTACGTAATGACTTGTTATCTCTCGCATCCCTGGGCATAAGCGACTCCATAAACGGGTTCTTa                                                                                                     <  2:4836644/150‑1 (MQ=255)
                                                  ttGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGTGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTa                                                                                                     >  2:4717776/1‑150 (MQ=255)
                                                  ttGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAAAAAAACAAAATCAGTACGTAATGACATGGTATATCTCTCATCCCAGGGCAAAATCGACTCCATAAACGGGTTCTTa                                                                                                     >  2:5422809/1‑150 (MQ=255)
                                                    gTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATg                                                                                                   >  1:2038903/1‑150 (MQ=255)
                                                     tAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGc                                                                                                  >  2:818632/1‑150 (MQ=255)
                                                        ccGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCtt                                                                                               >  2:2895241/1‑150 (MQ=255)
                                                         cGGTGGCTGGTTAAATGCCGACGCGACGGCAGAGGGAACGGATTTTCGCCCCGTCCTGCGAGGCTAACAAAACAAACTCAGTACTTAATGACTTGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTa                                                                                              <  2:555139/150‑1 (MQ=255)
                                                                  gTGAAAGGCCGACGCGACGGCAGTGGGAACGGATGTTCGCCCCGTCCTGCGCGGCGAACAAAACAAACTCAGTACGTAATGACTTGGTTTCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAgt                                                                                     <  2:4482571/150‑1 (MQ=255)
                                                                      aaTGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCt                                                                                 >  1:3621371/1‑150 (MQ=255)
                                                                        ggcctatgCGAGGGCTGCGGTAAGGGCTGTTCGCCCCGTCGGGCGTGGCTAAAAAAAAAAACTCATTACTTATTGACTTGGTATCTCCCGCACCCCTGGGAATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTAc                                                                               <  1:4385321/143‑1 (MQ=255)
                                                                         gCCGAAGCGAGGGCTGTGGGAACGTTTGTTCGCCCCGTCCTGCGTGGCTAACAAAAAAAAATCAGTACTTATTGACATGTTATCTCTGGAATCCCCGGGAATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTAcc                                                                              <  2:1544006/150‑1 (MQ=255)
                                                                          ccGAAGCGACGGCAGAGGGACCGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTGAGTTGTACGTGTCTACCa                                                                             >  2:3107162/1‑150 (MQ=255)
                                                                            gAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATg                                                                           >  2:4757363/1‑150 (MQ=255)
                                                                                cGACGGCAGAGGGAAGGGTTGTTCGCCCCGTCCTGCGTGGCTAAAAAAACAAACTCAGTACGTATTGACTTGTTATCTCTCGCAGCCCTGGCAATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTccc                                                                       <  1:3715048/150‑1 (MQ=255)
                                                                                cGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACTTGGTATCTCTCGCATCCCCGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTccc                                                                       <  2:3975840/150‑1 (MQ=255)
                                                                                     gCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACTTAAGGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAAc                                                                  <  2:5367326/150‑1 (MQ=255)
                                                                                       agtgggAACGGATGTTCGCCCCGTCCTGCGTGGCGAACAAAACAAACTCAGTACGTAATGACAGGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACaa                                                                <  1:348868/147‑1 (MQ=255)
                                                                                       agagGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACaa                                                                >  1:1819374/1‑150 (MQ=255)
                                                                                       agagGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACaa                                                                >  1:762986/1‑150 (MQ=255)
                                                                                          gggAACGGATGTTCGCCCCGTTCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAgtg                                                             >  1:3092672/1‑150 (MQ=255)
                                                                                           ggAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATGAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAgtgt                                                            >  2:2545240/1‑150 (MQ=255)
                                                                                           ggAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAgtgt                                                            >  1:3595109/1‑150 (MQ=255)
                                                                                              aCGTATGGTCGCCCCGTCCTGCGAGGCTAACAAAAAAAACTCATTACTTAATGACTTGGTATCTCCCGCAGCCCTGGGAATACGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCa                                                         <  1:867555/150‑1 (MQ=255)
                                                                                              aCGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCTGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCa                                                         <  2:2948437/150‑1 (MQ=255)
                                                                                                ggATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACt                                                       >  2:1364568/1‑150 (MQ=255)
                                                                                                ggATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCGTAGTTGTAAGTGTCGACCATGTCCCCGAACAAGTGTTCACt                                                       >  2:634250/1‑150 (MQ=255)
                                                                                                 tttgttcgcctcgcaggacggggcgaacaAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTa                                                      >  1:4313617‑M2/30‑150 (MQ=255)
                                                                                                   tgttcgcctcgcaggacggggcgaacaAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGc                                                                                                                                <  1:5167885‑M2/47‑1 (MQ=255)
                                                                                                     ttcgcctcgcaggacggggcgaacaAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTc                                                  >  2:3758115‑M2/26‑150 (MQ=255)
                                                                                                     ttcgcctcgcaggacggggcgaacaAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTc                                                  >  2:3834360‑M2/26‑150 (MQ=255)
                                                                                                     ttcgcctcgcaggacggggcgaacaAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGGTCTGATGCCTTAGTTGTAAGTGTCTACCCTGTCCCCGAACAAGTGTTCACTCTGTc                                                  >  1:1262617‑M2/26‑150 (MQ=255)
                                                                                                     TTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTCTCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTc                                                  <  1:5220524‑M1/150‑1 (MQ=255)
                                                                                                     TTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTc                                                  <  2:396354‑M1/150‑1 (MQ=255)
                                                                                                     TTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTc                                                  <  1:2354056‑M1/150‑1 (MQ=255)
                                                                                                      tcgcctcgcaggacggggcgaacaAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTcc                                                 >  1:2213758‑M2/25‑150 (MQ=255)
                                                                                                         CCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCg                                              <  2:1628061‑M1/150‑1 (MQ=255)
                                                                                                           ccGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGa                                            <  2:3435874/150‑1 (MQ=255)
                                                                                                                  gCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTaca                                     >  1:3880498/1‑150 (MQ=255)
                                                                                                                   cgcggcgaacaAAACAACCTCAGTCCGTAATGACCTGGTATCGCTCGCATCCCGGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTacac                                    <  1:706937‑M2/139‑1 (MQ=255)
                                                                                                                     tggctaacaAAACAAACTCAGTACTTAATGACATGGTATCTCTCGAAACCCTGGGCATACGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACAccc                                  <  1:991203‑M2/141‑1 (MQ=255)
                                                                                                                     cggcgaacaAAACAAACACAGTACGTAATGACATGGTATCTCGCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACAccc                                  <  2:2097575‑M2/141‑1 (MQ=255)
                                                                                                                       gcgaacaAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCa                                >  2:5116265‑M2/8‑150 (MQ=255)
                                                                                                                       gcgaacaAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCa                                >  2:867523‑M2/8‑150 (MQ=255)
                                                                                                                          aacaAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAg                             >  1:3765366‑M2/5‑150 (MQ=255)
                                                                                                                           acaAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAgg                            >  2:491985‑M2/4‑150 (MQ=255)
                                                                                                                           acaAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAgg                            >  2:1840278‑M2/4‑150 (MQ=255)
                                                                                                                           acaAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAgg                            >  2:5167920‑M2/4‑150 (MQ=255)
                                                                                                                           acaAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCATACACCCCAAAgg                            >  1:1589563‑M2/4‑150 (MQ=255)
                                                                                                                             aAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAgggg                          >  2:3509618‑M2/2‑150 (MQ=255)
                                                                                                                             aAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAgggg                          >  2:775417‑M2/2‑150 (MQ=255)
                                                                                                                                  aaacTCATTCCGTAATGACATGGTATCTCCCGCAGCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAgg                     <  1:3489982/150‑1 (MQ=255)
                                                                                                                                   aacTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAggg                    >  1:1896293/1‑150 (MQ=255)
                                                                                                                                      tCATTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGAc                 <  1:2605307/150‑1 (MQ=255)
                                                                                                                                          tACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCa             >  2:13338/1‑150 (MQ=255)
                                                                                                                                           aCGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCAc            >  2:4309854/1‑150 (MQ=255)
                                                                                                                                            cGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAATTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCAcc           <  1:4064837/150‑1 (MQ=255)
                                                                                                                                                 tGACATGGTATCTCTCCCATCCCCGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGcc      <  2:1106061/150‑1 (MQ=255)
                                                                                                                                                     aTGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCt  >  1:5319406/1‑150 (MQ=255)
                                                                                                                                                     aTGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCt  >  1:1040258/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCGGTCCTGAAGACCGGCGGCACCTTCCTGAGCCCAGCGCTGGAGCCACTTGTAGCCGGTGGCAGGTGAAATGCCGAAGCGACGGCAGAGGGAACGGATGTTCGCCCCGTCCTGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCT  >  NC_000913/281797‑282095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: